167 research outputs found
Cbl-b Positively Regulates Btk-mediated Activation of Phospholipase C-γ2 in B Cells
Genetic studies have revealed that Cbl-b plays a negative role in the antigen receptor–mediated proliferation of lymphocytes. However, we show that Cbl-b–deficient DT40 B cells display reduced phospholipase C (PLC)-γ2 activation and Ca2+ mobilization upon B cell receptor (BCR) stimulation. In addition, the overexpression of Cbl-b in WEHI-231 mouse B cells resulted in the augmentation of BCR-induced Ca2+ mobilization. Cbl-b interacted with PLC-γ2 and helped the association of PLC-γ2 with Bruton's tyrosine kinase (Btk), as well as B cell linker protein (BLNK). Cbl-b was indispensable for Btk-dependent sustained increase in intracellular Ca2+. Both NH2-terminal tyrosine kinase-binding domain and COOH-terminal half region of Cbl-b were essential for its association with PLC-γ2 and the regulation of Ca2+ mobilization. These results demonstrate that Cbl-b positively regulates BCR-mediated Ca2+ signaling, most likely by influencing the Btk/BLNK/PLC-γ2 complex formation
Current status of Japanese detectors
Current status of TAMA and CLIO detectors in Japan is reported in this
article. These two interferometric gravitational-wave detectors are being
developed for the large cryogenic gravitational wave telescope (LCGT) which is
a future plan for detecting gravitational wave signals at least once per year.
TAMA300 is being upgraded to improve the sensitivity in low frequency region
after the last observation experiment in 2004. To reduce the seismic noises, we
are installing new seismic isolation system, which is called TAMA Seismic
Attenuation System, for the four test masses. We confirmed stable mass locks of
a cavity and improvements of length and angular fluctuations by using two SASs.
We are currently optimizing the performance of the third and fourth SASs. We
continue TAMA300 operation and R&D studies for LCGT. Next data taking in the
summer of 2007 is planned.
CLIO is a 100-m baseline length prototype detector for LCGT to investigate
interferometer performance in cryogenic condition. The key features of CLIO are
that it locates Kamioka underground site for low seismic noise level, and
adopts cryogenic Sapphire mirrors for low thermal noise level. The first
operation of the cryogenic interferometer was successfully demonstrated in
February of 2006. Current sensitivity at room temperature is close to the
target sensitivity within a factor of 4. Several observation experiments at
room temperature have been done. Once the displacement noise reaches at thermal
noise level of room temperature, its improvement by cooling test mass mirrors
should be demonstrated.Comment: 6 pages, 5 figures, Proceedings of GWDAW-1
Apical Cl-/HCO3- exchanger stoichiometry in the modeling of HCO3- transport by pancreatic duct epithelium
Pancreatic duct cells secrete a HCO3--rich (~140 mM) fluid. Using a computer model of the pancreatic duct, Sohma, et al. have demonstrated that the activity of a Cl-/ HCO3- exchanger with a 1 : 1 stoichiometry at the apical membrane would have to be suppressed in order to achieve such a HCO3--rich secretion. Recently the apical exchanger in pancreatic ducts has been identified as SLC26A6 and this probably mediates most of Cl--dependent HCO3- secretion across the apical membrane. SLC26A6 is reported to mediate electrogenic Cl-/2HCO3- exchange when expressed in Xenopus oocytes. To assess the implications of this 1 : 2 stoichiometry for HCO3- secretion, we have reconstructed the Sohma model using MATLAB/Simulink. To do this we have formulated an expression for the turnover rate of Cl-/2HCO3- exchange using network thermodynamics and we have estimated the constants from published experimental data. Preliminary data suggest that the 1 : 2 stoichiometry of SLC26A6 would favor HCO3- secretion at higher concentrations
Zigzag-Elongated Fused π-Electronic Core: A Molecular Design Strategy to Maximize Charge-Carrier Mobility
Printed and flexible electronics requires solution‐processable organic semiconductors with a carrier mobility (μ) of ≈10 cm2 V−1 s−1 as well as high chemical and thermal durability. In this study, chryseno[2,1‐b:8,7‐b′]dithiophene (ChDT) and its derivatives, which have a zigzag‐elongated fused π‐electronic core (π‐core) and a peculiar highest occupied molecular orbital (HOMO) configuration, are reported as materials with conceptually new semiconducting π‐cores. ChDT and its derivatives are prepared by a versatile synthetic procedure. A comprehensive investigation reveals that the ChDT π‐core exhibits increasing structural stability in the bulk crystal phase, and that it is unaffected by a variation of the transfer integral, induced by the perpetual molecular motion of organic materials owing to the combination of its molecular shape and its particular HOMO configuration. Notably, ChDT derivatives exhibit excellent chemical and thermal stability, high charge‐carrier mobility under ambient conditions (μ ≤ 10 cm2 V−1 s−1), and a crystal phase that is highly stable, even at temperatures above 250 °C
Anelastic strain recovery reveals extension across SW Japan subduction zone
Sediment dominated convergent margins typically
record substantial horizontal shortening often associated
with great earthquakes. The convergent margin south of
Japan is arguably one of the most extensively investigated
margins and previous studies have documented extensive
evidence for accretion and horizontal shortening. Here, we
show results from anelastic strains recovered from three
partially lithified sediment samples (40~ porosities)
across the southwest Japan accretionary prism and
propose that the margin is dominated by horizontal
extension rather than compression. The anelastic strain
results are also consistent with stress directions interpreted
from two independent techniques - bore hole breakout
orientations and core-scale fault data. We interpret this
unexpected result to reflect geologically recent underthrusting
of a thick sediment package and concomitant
weakening of the decollement
Coincidence analysis to search for inspiraling compact binaries using TAMA300 and LISM data
Japanese laser interferometric gravitational wave detectors, TAMA300 and
LISM, performed a coincident observation during 2001. We perform a coincidence
analysis to search for inspiraling compact binaries. The length of data used
for the coincidence analysis is 275 hours when both TAMA300 and LISM detectors
are operated simultaneously. TAMA300 and LISM data are analyzed by matched
filtering, and candidates for gravitational wave events are obtained. If there
is a true gravitational wave signal, it should appear in both data of detectors
with consistent waveforms characterized by masses of stars, amplitude of the
signal, the coalescence time and so on. We introduce a set of coincidence
conditions of the parameters, and search for coincident events. This procedure
reduces the number of fake events considerably, by a factor
compared with the number of fake events in single detector analysis. We find
that the number of events after imposing the coincidence conditions is
consistent with the number of accidental coincidences produced purely by noise.
We thus find no evidence of gravitational wave signals. We obtain an upper
limit of 0.046 /hours (CL ) to the Galactic event rate within 1kpc from
the Earth. The method used in this paper can be applied straightforwardly to
the case of coincidence observations with more than two detectors with
arbitrary arm directions.Comment: 28 pages, 17 figures, Replaced with the version to be published in
Physical Review
Results of the search for inspiraling compact star binaries from TAMA300's observation in 2000-2004
We analyze the data of TAMA300 detector to search for gravitational waves
from inspiraling compact star binaries with masses of the component stars in
the range 1-3Msolar. In this analysis, 2705 hours of data, taken during the
years 2000-2004, are used for the event search. We combine the results of
different observation runs, and obtained a single upper limit on the rate of
the coalescence of compact binaries in our Galaxy of 20 per year at a 90%
confidence level. In this upper limit, the effect of various systematic errors
such like the uncertainty of the background estimation and the calibration of
the detector's sensitivity are included.Comment: 8 pages, 4 Postscript figures, uses revtex4.sty The author list was
correcte
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