22 research outputs found
Cytoplasmic MTOCs control spindle orientation for asymmetric cell division in plants
Proper orientation of the cell division axis is critical for asymmetric cell divisions that underpin cell differentiation. In animals, centrosomes are the dominant microtubule organizing centers (MTOC) and play a pivotal role in axis determination by orienting the mitotic spindle. In land plants that lack centrosomes, a critical role of a microtubular ring structure, the preprophase band (PPB), has been observed in this process; the PPB is required for orienting (before prophase) and guiding (in telophase) the mitotic apparatus. However, plants must possess additional mechanisms to control the division axis, as certain cell types or mutants do not form PPBs. Here, using live imaging of the gametophore of the moss Physcomitrella patens, we identified acentrosomal MTOCs, which we termed "gametosomes," appearing de novo and transiently in the prophase cytoplasm independent of PPB formation. We show that gametosomes are dispensable for spindle formation but required for metaphase spindle orientation. In some cells, gametosomes appeared reminiscent of the bipolar MT "polar cap" structure that forms transiently around the prophase nucleus in angiosperms. Specific disruption of the polar caps in tobacco cells misoriented the metaphase spindles and frequently altered the final division plane, indicating that they are functionally analogous to the gametosomes. These results suggest a broad use of transient MTOC structures as the spindle orientation machinery in plants, compensating for the evolutionary loss of centrosomes, to secure the initial orientation of the spindle in a spatial window that allows subsequent fine-tuning of the division plane axis by the guidance machinery
MgB2 coated superconducting tapes with high critical current densities fabricated by hybrid physical-chemical vapor deposition
The MgB2 coated superconducting tapes have been fabricated on textured Cu (0
0 1) and polycrystalline Hastelloy tapes using coated conductor technique,
which has been developed for the second generation high temperature
superconducting wires. The MgB2/Cu tapes were fabricated over a wide
temperature range of 460-520 {\deg}C by using hybrid physical-chemical vapor
deposition (HPCVD) technique. The tapes exhibited the critical temperatures
(Tc) ranging between 36 and 38 K with superconducting transition width
(\DeltaTc) of about 0.3-0.6 K. The highest critical current density (Jc) of
1.34 \times 105 A/cm2 at 5 K under 3 T is obtained for the MgB2/Cu tape grown
at 460^{\circ}C. To further improve the flux pinning property of MgB2 tapes,
SiC is coated as an impurity layer on the Cu tape. In contrast to pure MgB2/Cu
tapes, the MgB2 on SiC-coated Cu tapes exhibited opposite trend in the
dependence of Jc with growth temperature. The improved flux pinning by the
additional defects created by SiC-impurity layer along with the MgB2 grain
boundaries lead to strong improvement in Jc for the MgB2/SiC/Cu tapes. The
MgB2/Hastelloy superconducting tapes fabricated at a temperature of 520 {\deg}C
showed the critical temperatures ranging between 38.5 and 39.6 K. We obtained
much higher Jc values over the wide field range for MgB2/Hastelloy tapes than
the previously reported data on other metallic substrates, such as Cu, SS, and
Nb. The Jc values of Jc(20 K, 0 T) \sim5.8 \times 106 A/cm2 and Jc(20 K, 1.5 T)
~2.4 \times 105 A/cm2 is obtained for the 2-\mum-thick MgB2/Hastelloy tape.
This paper will review the merits of coated conductor approach along with the
HPCVD technique to fabricate MgB2 conductors with high Tc and Jc values which
are useful for large scale applications.Comment: 17 pages, 12 figures, accepted for publication in Current Applied
Physics as a review articl
Gas chromatography flow rates for determining deuterium/hydrogen ratios of natural gas by gas chromatography/high-temperature conversion/isotope ratio mass spectrometry
Seismic structure of the source region of the 2007 Chuetsu-oki earthquake revealed by offshore-onshore seismic survey: Asperity zone of intraplate earthquake delimited by crustal inhomogeneity
http://www.godac.jamstec.go.jp/darwin/cruise/kairei/kr07-e01/