Régénération assistée du karité (Vitellaria paradoxa C. F. Gaertn.) dans les parcs agroforestiers au Burkina Faso

Le karité (Vitellaria paradoxa C. F. Gaertn.) présente une importance sociale, culturelle, économique et alimentaire pour le Burkina Faso où il occupe la quatrième place dans les produits d’exportation. La pérennité de cette espèce dont les populations rurales sont tributaires, est compromise par de multiples phénomènes dont le manque de régénération, les pratiques culturales et la coupe abusive du bois pour satisfaire les besoins énergétiques. L’objectif de cette étude est de proposer des méthodes adéquates pour rajeunir les parcs à karité au Burkina Faso. Cinq sites de recherche ont été choisis suivant un gradient phytogéographique : Sobaka, Noumoudara et Kakoumana (secteur sud soudanien), Gonsé (secteur nord soudanien) et Bouria (secteur sub sahélien). Les essais effectués dans chaque parcelle choisie, ont comporté trois répétitions et 7 traitements, représentés par les différentes techniques de régénération (plantation, transplantation, semis direct libre, semis dans les buissons, régénération naturelle assistéeRNA-, induction de drageon, induction de pousse adventive). Les résultats indiquent que le taux de survie de la régénération naturelle assistée est audessus de 70% après deux années de suivi. Les plantations et les semis directs dans les buissons sont aussi des techniques efficaces pour la régénération/restauration des parcs à karité, avec respectivement des taux de survie de 13,33% et 6,67% dans le secteur sub sahélien, 12,22% et 6% dans le nord soudanien, et 55,56% et 25,33% dans le sud soudanien après la troisième année de suivi. En conclusion, la régénération assistée est la technique la plus efficiente pour restaurer les parcs à karité. The Shea (Vitellaria paradoxa C. F. Gaertn.) is of enormous social, cultural, economic and nutritional importance for Burkina Faso where it ranks fourth in exports. The sustainability of this species on which rural populations depend is compromised by multiple phenomena, including the lack of regeneration of shea tree and the abusive cutting of wood to meet energy needs. This work aimed to propose adequate methods for rejuvenating shea parkland in Burkina Faso. Five sites distributed along a phytogeographic gradient were selected for tests : Sobaka, Noumoudara and Kakoumana (South-Sudanian phytogeographic zone), Gonsé (NorthSudanian phytogeographic zone) and Bouria (sub-Sahelian phytogeographic zone). The field trials included three repetitions by regeneration technique (planting, transplanting, direct sowing free, sowing in bushes and assisted natural regeneration -ANR-, sucker induction, induction of adventitious growth). The results indicate that the survival rate of assisted natural regeneration is above 70% after two years of follow-up. Planting and direct seeding in bushes are also effective techniques for the regeneration / restoration of shea tree parks with respectively survival rates of 13.33% and 6.67% in the sub sahelian sector, 12.22% and 6% in north sudanian, and 55.56% and 25.33% in south sudanian, after the third year of monitoring. In conclusion, in order to restore the shea parks, all seedlings regardless of their origins, will need to be assisted i

Effect of seasonal malaria chemoprevention plus azithromycin on Plasmodium falciparum transmission: gametocyte infectivity and mosquito fitness.

BACKGROUND: Seasonal malaria chemoprevention (SMC) consists of administration of sulfadoxine-pyrimethamine (SP) + amodiaquine (AQ) at monthly intervals to children during the malaria transmission period. Whether the addition of azithromycin (AZ) to SMC could potentiate the benefit of the intervention was tested through a double-blind, randomized, placebo-controlled trial. The effect of SMC and the addition of AZ, on malaria transmission and on the life history traits of Anopheles gambiae mosquitoes have been investigated. METHODS: The study included 438 children randomly selected from among participants in the SMC + AZ trial and 198 children from the same area who did not receive chemoprevention. For each participant in the SMC + AZ trial, blood was collected 14 to 21 days post treatment, examined for the presence of malaria sexual and asexual stages and provided as a blood meal to An. gambiae females using a direct membrane-feeding assay. RESULTS: The SMC treatment, with or without AZ, significantly reduced the prevalence of asexual Plasmodium falciparum (LRT X22 = 69, P < 0.0001) and the gametocyte prevalence (LRT X22 = 54, P < 0.0001). In addition, the proportion of infectious feeds (LRT X22 = 61, P < 0.0001) and the prevalence of oocysts among exposed mosquitoes (LRT X22 = 22.8, P < 0.001) was reduced when mosquitoes were fed on blood from treated children compared to untreated controls. The addition of AZ to SPAQ was associated with an increased proportion of infectious feeds (LRT X21 = 5.2, P = 0.02), suggesting a significant effect of AZ on gametocyte infectivity. There was a slight negative effect of SPAQ and SPAQ + AZ on mosquito survival compared to mosquitoes fed with blood from control children (LRTX22 = 330, P < 0.0001). CONCLUSION: This study demonstrates that SMC may contribute to a reduction in human to mosquito transmission of P. falciparum, and the reduced mosquito longevity observed for females fed on treated blood may increase the benefit of this intervention in control of malaria. The addition of AZ to SPAQ in SMC appeared to enhance the infectivity of gametocytes providing further evidence that this combination is not an appropriate intervention

Nationwide Trends in Bacterial Meningitis before the Introduction of 13-Valent Pneumococcal Conjugate Vaccine-Burkina Faso, 2011-2013.

BACKGROUND: Following introduction of Haemophilus influenzae type b vaccine in 2006 and serogroup A meningococcal conjugate vaccine in 2010, Streptococcus pneumoniae (Sp) became the leading cause of bacterial meningitis in Burkina Faso. We describe bacterial meningitis epidemiology, focusing on pneumococcal meningitis, before 13-valent pneumococcal conjugate vaccine (PCV13) introduction in the pediatric routine immunization program in October 2013. METHODS: Nationwide population-based meningitis surveillance collects case-level demographic and clinical information and cerebrospinal fluid (CSF) laboratory results. Sp infections are confirmed by culture, real-time polymerase chain reaction (rt-PCR), or latex agglutination, and CSF serotyped using real-time and conventional PCR. We calculated incidence rates in cases per 100,000 persons, adjusting for age and proportion of cases with CSF tested at national reference laboratories, and case fatality ratios (CFR). RESULTS: During 2011-2013, 1,528 pneumococcal meningitis cases were reported. Average annual adjusted incidence rates were 26.9 (<1 year), 5.4 (1-4 years), 7.2 (5-14 years), and 3.0 (≥15 years). Overall CFR was 23% and highest among children aged <1 year (32%) and adults ≥30 years (30%). Of 1,528 cases, 1,036 (68%) were serotyped: 71% were PCV13-associated serotypes, 14% were non-PCV13-associated serotypes, and 15% were non-typeable by PCR. Serotypes 1 (45%) and 12F/12A/12B/44/46 (8%) were most common. Among children aged <1 year, serotypes 5 (15%), 6A/6B (13%) and 1 (12%) predominated. CONCLUSIONS: In Burkina Faso, the highest morbidity and mortality due to pneumococcal meningitis occurred among children aged <1 year. The majority of cases were due to PCV13-associated serotypes; introduction of PCV13 should substantially decrease this burden

Initial validation of a simulation model for estimating the impact of serogroup A Neisseria meningitidis vaccination in the African meningitis belt.

We previously developed a mathematical simulation of serogroup A Neisseria meningitidis (NmA) transmission in Burkina Faso, with the goal of forecasting the relative benefit of different vaccination programs. Here, we revisit key structural assumptions of the model by comparing how accurately the different assumptions reproduce observed NmA trends following vaccine introduction. A priori, we updated several of the model's parameters based on recently published studies. We simulated NmA disease under different assumptions about duration of vaccine-induced protection (including the possibility that vaccine-induced protection may last longer than natural immunity). We compared simulated and observed case counts from 2011-2017. We then used the best-fit model to forecast the impact of different vaccination strategies. Our updated model, with the assumption that vaccine-induced immunity lasts longer than immunity following NmA colonization, was able to reproduce observed trends in NmA disease. The updated model predicts that, following a mass campaign among persons 1-29 years of age, either routine immunization of 9 month-old children or periodic mini-campaigns among children 1-4 years of age will lead to sustained control of epidemic NmA in Burkina Faso. This validated model can help public health officials set policies for meningococcal vaccination in Africa

Dengue Fever in Burkina Faso, 2016

We report 1,327 probable cases of dengue in Burkina Faso in 2016. Of 35 serum samples tested by a trioplex test, 19 were confirmed dengue virus (DENV)‒positive: 11 DENV-2, 6 DENV-3, 2 nontypeable, and 1 DENV-2/DENV-3 co-infection. Molecular testing should be conducted to correctly identify causative agents in this complex infectious disease landscape

Flow diagram of the national case-based meningitis surveillance system, Burkina Faso, 2011–2013.

<p>Abbreviation: rt-PCR, real-time polymerase chain reaction. *Specimens can be tested by multiple methods.</p

Epidemic curve of all suspected, probable, and confirmed meningitis cases by month, Burkina Faso, 2011–2013.

<p>Epidemic curve of all suspected, probable, and confirmed meningitis cases by month, Burkina Faso, 2011–2013.</p

Annual incidence (cases per 100,000 persons) of suspected, probable, and confirmed meningitis, Burkina Faso, 2011–2013.

<p>Annual incidence (cases per 100,000 persons) of suspected, probable, and confirmed meningitis, Burkina Faso, 2011–2013.</p

Cases reported through national meningitis surveillance by age group and etiology, Burkina Faso, 2011–2013.

<p>Cases reported through national meningitis surveillance by age group and etiology, Burkina Faso, 2011–2013.</p