The cytotoxic evaluation of mineral trioxide aggregate and bioaggregate in the subcutaneous connective tissue of rats

Objectives: The purpose of this study was to evaluate and compare the cytotoxic effects of ProRoot MTA and DiaRoot BA, a bioceramic nanoparticulate cement, on subcutaneous rat tissue. Study D esign: Fifty Sprouge Dawley rats were used in this study. Polyethylene tubes filled with ProRoot MTA and DiaRoot BioAggregate, along with a control group of empty, were implanted into dorsal connective tissue of rats for 7, 15, 30, 60, and 90 days. After estimated time intervals the rats were sacrificed. The specimens were fixed, stained with hematoxylin and eosin, and then evaluated under a light microscope for inflammatory reactions and mineralization. Results: All groups evoked a severe to moderate chronic inflammatory reaction at 7 and 15 days, which decreased with time. Both the MTA and BioAggregate groups showed similar inflammatory reactions, except at 90 days when MTA showed statistically significant greater inflammation (p>0.05). The MTA group showed foreign body reaction at all times. Compared to BioAggregate, MTA showed significantly more foreign body reaction at 60 and 90 days (p<0.0001). After 30 days foreign body reaction of BioAggregate decreased significantly. Both MTA and BioAggregate groups showed similar necrosis at 7 and 15 days (p=0.094 and p=0.186 respectively). No necrosis was observed after 15 days. Similarly there was no fibrosis after 30 days for both MTA and BioAggregate groups (p>0.05). Conclusions: Since DiaRoot BioAggregate showed significantly better results than MTA, we can conclude that it is more biocompatible. However, further studies are required to confirm this result

Prevalence, classification and dental treatment requirements of dens invaginatus by cone-beam computed tomography

Background. This study aimed the evaluation of the prevalence, characteristics, types of dens invaginatus (DI) and co-observed dental anomalies to understand dental treatment requirements in anterior teeth that are susceptible to developmental anomalies by using cone-beam computed tomography (CBCT). Methods. In this retrospective study, the anterior teeth of 958 patients were evaluated by using CBCT for the presence of DI. The demographic features, types of DI and treatment requirements were also recorded. The association between sex and the presence of DI was evaluated using chi-squared test. Results. Seventy-three DI anomalies were detected in the anterior teeth of 49 patients (18 females, 31 males). The frequency of DI was 5.11% and the most frequently involved teeth were lateral (57.53%). Forty-six teeth were classified as Type I (63.01%), 24 as Type II (32.87%), and three as Type III (4.10%). Apical pathosis was found to be 20.54% in all DIs detected and accounted for all Type III and one-third of Type II. Conclusions. CBCT imaging can be effective in the detection of dental anomalies such as DI and planning for root canal therapy and surgical treatments. Prophylactic interventions might be possible to prevent apical pathosis with the data obtained from CBCT images

The role of covid-19 anxiety and intolerance of uncertainty in predicting resilience

Resilience is one of the protective factors for mental health. The aim of this study is to reveal the role of COVID-19 anxiety and intolerance of uncertainty in predicting resilience in adulthood. To this end, data were collected from 533 adults (359 women-174 men). A hierarchical regression analysis was performed, controlling for the effects of gender and presence of a chronic disease on the prediction of resilience. Results showed that intolerance of uncertainty and COVID-19 anxiety were statistically significant predictors of resilience. Gender and presence of a chronic disease were not statistically significant in predicting resilience. These results indicate that intolerance of uncertainty plays an important role in explaining resilience

Endodontic and Surgical Combined Treatment of Case with Root Resorption: Case Report

This report presents a case treated both endodontically and surgically of a mandibular left canine tooth with two seperate inflammatory resorptive defects, one perforating the apical one-third. Following obturation with hybrid tecnique, mucoperiostal flap was performed and MTA was applied into the perforated resorptive cavity. At 1-year follow-up, the tooth was free of symptoms and complete healing was observed around the root in radiographic examination. At 2-year follow-up, the tooth was asymptomatic in clinically and radiologically. For postoperatif results of the treatment, sealing of perforation site with material such as MTA which has biocompatibility and good sealing properties may result in good clinical prognosis

A high-throughput microfluidic rare cell enrichment system based on dielectrophoresis and filtering

In this study, a MEMS-based microfluidic device combining DEP-based cell manipulation with size-based filtration for the enrichment of CTCs from blood with high-throughput was developed. Positive-DEP (pDEP) force and the hydrodynamic force have been used to fine-tune the cell movement over the planar electrodes (sliding) at a high flow rate (30 mu l/min). While smaller sized RBCs passed through the gaps and were directed to the waste channels, cancer cells (K562 cells) were filtered and directed to the cancer cell outlet. A cell enrichment factor and depletion rate of RBCs were calculated as 1.45 and 60%, respectively

Exploring the relationship between cytoplasmic ion content variation and multidrug resistance in cancer cells via ion-release based impedance spectroscopy

\u3cp\u3eThis study aimed to measure and compare the cytoplasmic ion concentrations of the parental cells and their drug resistant progenies. High-level laboratory and clinically-relevant multidrug resistance (MDR) cell line models were developed and investigated for K562 and CCRF-CEM cells, respectively. Measurements were achieved using a modified version of ion release-based impedance spectroscopy (IRbIS), where the average total ion concentration per cell was determined by measuring the variations in solution conductance after the lysis of a population with a known initial cell count. Results showed that the average of cytoplasmic ion concentration for K562/imaR cells was almost 1.8 times higher than that of K562/wt cells with a standard error of mean (SEM) lower than 7%, having a significant difference at p = 0.05 level. CCRF-CEM/doxR cells had almost a 1.2-fold increase in the ion concentration compared with CCRF-CEM/wt cells with an SEM smaller than 11%. Unlike K562 cells, this was not a significant variation at p = 0.05 level. Having insignificant SEMs proved the validity of this modified implementation of IRbIS measurements. We showed that our new approach enables to identify variations in cytoplasmic ion concentration per cell and to understand the mechanisms driving the toxicity and the development of drug resistance to chemotherapeutic agents.\u3c/p\u3

ANALYSIS OF THE DIELECTROPHORETIC (DEP) SPECTRA OF BIOLOGICAL CELLS

This study presents an approach for analyzing the dielectrophoretic (DEP) spectra of biological cells without ascertaining their membrane and cytoplasmic properties. DEP spectrum device with reciprocal V-shaped planar-electrodes was utilized to obtain DEP spectra information of cells. Integrating electric field simulations based on a strong theory with the experimental data enables determination of the DEP spectrum at a range of frequencies, for the first in the literature. The proposed method is tested with MCF7 breast cancer cells and DEP spectra are obtained for 15 different frequencies in the range from 100 kHz to 50 MHz at 10 V-pp

Aripiprazole Augmentation of Clomipramine Therapy in Treatment-Resistant

: Vaka serisi Obsesif-kompulsif bozukluk (OKB) yineleyen, istenmeyen düşünceler ve törensel davranışlarla giden, belirgin sıkıntı ve işlev kaybına neden olan kronik bir hastalıktır. Yapılan çalışmalarda OKB nöropatogenezinde serotonerjik ve dopaminerjik mekanizmaların rol oynadığına ilişkin çeşitli kanıtlar gösterilmiştir. Tedavisinde ilk tercih ilaç olarak Seçici Serotonin Geri Alım İnhibitörleri (SSGİ) kabul edilmektedir, ancak buna karşın SSGİ ile tedavi edilen hastaların yaklaşık %40-60'ında tedavi direnci görülebilmektedir. Tedaviye dirençli OKB'de antidepresanların atipik antipsikotiklerle augmentasyonu önemli bir tedavi seçeneğidir. Biz de bu yazımızda; tedaviye dirençli 5 OKB hastasının klomipramin tedavisine aripiprazolün 10-30mg/gün dozlarının eklenmesiyle tedavide olumlu yanıtlar elde ettiğimiz olgularını sunmayı amaçladıkObsessive-compulsive disorder (OCD) is a chronic disorder characterized by recurrent intrusive thoughts and repetitive rituals, causing significant distress and functional loss. Studies show evidence about serotonergic and dopaminergic mechanisms in neuropathogenesis of OCD. Selective serotonin re-uptake inhibitors (SSRI) are considered first line treatment in OCDs, but treatment resistance may occur in 40-60% of cases treated with SSRIs. Augmentation of antidepressants with atypical antipsychotics is an important treatment option in treatment-resistant patients with OCD. In this article, we aimed to present five OCD cases with treatment-resistance in which we obtained good outcomes, with addition of aripiprazole 10-30mg per day to clomipramine therap

Evaluation of Virulence Factors and Antifungal Susceptibility in Yeast Isolates from Postmortem Specimens

Invasive fungal infections are a leading cause of morbidity and mortality in immunocompromised patients, especially in cases requiring a prolonged stay in the intensive care unit. A total of 99 yeast strains were isolated from 42 postmortem cases. In this study, virulence factors and antifungal susceptibility of these species were evaluated. The isolates were identified as Candida albicans (54), C. tropicalis (15), C. glabrata (12), C. parapsilosis (6), C. lipolytica (3), C. utilis (3), C. krusei (2), C. kefyr (1), and Cryptococcus neoformans (3). The most commonly isolated species was C. albicans, and no resistant species were determined. Despite the equal number of specimens, no secretion of significant virulence factors was associated with the postmortem specimen in the Candida species. Postmortem fungal investigations in forensic autopsies are useful in explaining cause of death in such cases, also may lead to protocols for the treatment of fungal infections and contribute to fungal pathogenesis and epidemiological data