Определение концентрации холестерина и триглицеридов в экстраклеточных везикулах сыворотки крови с помощью коммерческих наборов

Exosomes and microvesicles, collectively referred to as small extracellular vesicles (sEV) are vesicles with an average size of about 100-150 nm. Currently, the role of sEV in various aspects of signaling in the body is being actively investigated; in addition, sEV can often serve as markers of various pathologies. The active study of the sEV composition is continuing. In this study we have demonstrated that in sEV it is possible to determine cholesterol and triglycerides concentration by using commercial kits designed for serum. The technique was tested on sEV from the blood of patients diagnosed with depression and on healthy volunteers. No differences were found in the concentration of cholesterol and triglycerides in mEV from the blood serum of depressed patients and the control group. The concentration of cholesterol and triglycerides in the samples is several times higher than the sensitivity threshold of the methods set by the manufacturer of the kits.Экзосомы и микровезикулы, совместно называемые малые экстраклеточные везикулы (мЭВ), представляют собой везикулы со средним размером около 100-150 нм. В настоящее время активно исследуется роль мЭВ в самых разных аспектах сигналинга в организме, кроме того, часто мЭВ могут служить маркерами разных патологий. Продолжается активное изучение состава мЭВ. В данной работе мы показали, что в мЭВ можно определить концентрацию холестерина и триглицеридов с помощью коммерческих наборов, предназначенных для сыворотки крови. Методика была опробована на мЭВ из крови пациентов с диагнозом депрессия и на здоровых добровольцах. Различий в концентрации холестерина и триглицеридов в мЭВ из сыворотки крови пациентов с депрессией и контрольной группы найдено не было. Концентрация холестерина и триглицеридов в образцах в несколько раз превосходит порог чувствительности методов, установленный производителем наборов

Binding Energy of Charged Excitons in ZnSe-based Quantum Wells

Excitons and charged excitons (trions) are investigated in ZnSe-based quantum well structures with (Zn,Be,Mg)Se and (Zn,Mg)(S,Se) barriers by means of magneto-optical spectroscopy. Binding energies of negatively () and positively (X+) charged excitons are measured as functions of quantum well width, free carrier density and in external magnetic fields up to 47 T. The binding energy of shows a strong increase from 1.4 to 8.9 meV with decreasing quantum well width from 190 to 29 A. The binding energies of X+ are about 25% smaller than the binding energy in the same structures. The magnetic field behavior of and X+ binding energies differ qualitatively. With growing magnetic field strength, increases its binding energy by 35-150%, while for X+ it decreases by 25%. Zeeman spin splittings and oscillator strengths of excitons and trions are measured and discussed

Youth physical education and sports: visions of future, problems and prospects

Objective of the study was to identify psychological and pedagogical features of perception of the future in adolescents during their physical education and sports activities. Methods and structure of the study. The study involved the 17-20 year-old full-time students of Smolensk Academy of Professional Education (Regional State Budget Professional Educational Institution SmolAPE), who were engaged in various sports (n=47). Creativity as a personality trait was tested in the 15-16 year-old male (n=18) and female (n=17) schoolchildren (tenth-graders) of Smolensk, who were engaged in team sports. Results of the study. We determined the overall level of meaning in life, total life satisfaction and irrational attitudes of university students, as well as the correlation between the scales of irrational attitudes and levels of life satisfaction index. Conclusion. The study showed that in the 10th grade, it was the hyperthymic type of accentuation of personality traits that prevailed in the students (34%). 23% of sporting school graduates belonged to the cyclothymic type of accentuation, and 20% - to the emotive one

Two-loop Anomalous Dimensions of Heavy Baryon Currents in Heavy Quark Effective Theory

We present results on the two-loop anomalous dimensions of the heavy baryon HQET currents $J=(q^TC\Gamma\tau q)\Gamma'Q$ with arbitrary Dirac matrices $\Gamma$ and $\Gamma'$. From our general result we obtain the two-loop anomalous dimensions for currents with quantum numbers of the ground state heavy baryons $\Lambda_Q$, $\Sigma_Q$ and $\Sigma_Q^*$. As a by-product of our calculation and as an additional check we rederive the known two-loop anomalous dimensions of mesonic scalar, pseudoscalar, vector, axial vector and tensor currents $(J=\bar q\Gamma q)$ in massless QCD as well as in HQET.Comment: 21 pages, LaTeX, 2 figures are included in PostScript forma

Psychological-pedagogical and medical-biological peculiarities of sports selection in cyclic sports

In the system of sports training, assessment of the psychological, pedagogical and medical-biological characteristics of schoolchildren and students is necessary, when the body-movement characteristics and hereditary effects on the organism are corroborated by the relationship between the achievements of each subject in the same test shown in different years.В системе спортивной тренировки необходима оценка психолого-педагогических и медико-биологических особенностей школьников и студентов, когда телесно-двигательные характеристики и наследственные влияния на организм подтверждаются взаимосвязью между достижениями каждого испытуемого в одном и том же тесте, показанном в разные годы

Physical and sports activity under self–sufficiency and profitability

In the article, the authors reveal the characteristics of physical education and sports activity in the frame of pedagogical process, services rendered under multi-level education system. The issues of physical education, which becomes increasingly interesting for the commercial sector of economics, are covered. The conditions of using sports facilities for preparing sports reserve which are connected with the problems of selecting children for chosen sports and using effective means and methods of training at all stages of long-term preparation are highlighted. The basic selection criteria for young volleyball players and forecasting their potential opportunities based on morphological indicators and speed-strength qualities are given. The characteristics of the rates of biological maturation that affect physical development, level of physical fitness and young athletes’ sports achievements are given.В данной статье авторы раскрывают особенности физкультурно-спортивной деятельности с позиций педагогического процесса, сервисных услуг в процессе функционирования многоуровневой системы образования. Освещены вопросы физического воспитания, которое все больше входит в круг интересов коммерческого сектора экономики. Рассмотрены условия использования спортивных сооружений для подготовки спортивного резерва, что отражает проблемы отбора детей для занятий избранным видом спорта и применение эффективных средств и методов тренировки на всех этапах многолетней подготовки. Приведены основные критерии отбора юных волейболистов и прогнозирования их потенциальных возможностей на основе морфологических показателей и скоростно-силовых качеств. Дана характеристика темпам биологического созревания, которые влияют на физическое развитие, уровень физической подготовленности и спортивные достижения юных спортсменов

Calculations of binding energies and masses of heavy quarkonia using renormalon cancellation

We use various methods of Borel integration to calculate the binding ground energies and masses of b-bbar and t-tbar quarkonia. The methods take into account the leading infrared renormalon structure of the hard+soft part of the binding energies E(s), and of the corresponding quark pole masses m_q, where the contributions of these singularities in M(s) = 2 m_q + E(s) cancel. Beforehand, we carry out the separation of the binding energy into its hard+soft and ultrasoft parts. The resummation formalisms are applied to expansions of m_q and E(s) in terms of quantities which do not involve renormalon ambiguity, such as MSbar quark mass, and alpha_s. The renormalization scales are different in calculations of m_q, E(s) and E(us). The MSbar mass of b quark is extracted, and the binding energies of t-tbar and the peak (resonance) energies for (t+tbar) production are obtained.Comment: 23 pages, 8 double figures, revtex4; the version to appear in Phys.Rev.D; extended discussion between Eqs.(25) and (26); the paragraph between Eqs.(32) and (33) is new and explains the numerical dependence of the residue parameter on the factorization scale; several new references were added; acknowledgments were modified; the numerical results are unchange

СОВРЕМЕННЫЕ ТЕХНОЛОГИИ В ХИРУРГИЧЕСКОМ ЛЕЧЕНИИ ПОСЛЕДСТВИЙ ТРАВМЫ ЧЕРЕПА И ГОЛОВНОГО МОЗГА

The paper presents main types of surgically relevant posttraumatic lesions in 4136 patients with skull vault as well as skull base defects, craniofacial deformities, recurrent CSF leaks, arterio-venous fistulas, aneurysms and pseudoaneurysms etc. Classification of TBI sequelae and complications as well as its clinical course grading is presented. The use of modern neuroimaging techniques for studying pathophysiologic mechanisms and complications of TBI has been demonstrated. Special emphasis was given to minimally invasive and reconstructive surgery; computer modeling with subsequent full-copy stereolitographic laser implant setup was shown which is of great importance in cases of large and complex skull base and craniofacial deformities. Patient selection for transcranial and endonasal CSF leak closure techniques was justified. Treatment of post-traumatic vascular injuries using Serbinenko balloon-catheters as well as modern techniques such as stents, coils and embolization has been demonstrated.  В сообщении (созданном по материалам доклада, представленного на Сессии общего собрания РАМН, Санкт-Петербург, 14−15 июня 2012 г.) представлены основные хирургически значимые формы посттравматической патологии у 4136 пациентов с дефектами и деформациями костей свода, основания черепа и лицевого скелета, рецидивирующей ликвореей, артериосинусными соустьями, истинными и ложными аневризмами и др. Разработана классификация последствий и осложнений черепно-мозговой травмы, а также периодизация ее клинического течения. Раскрыты возможности современных нейровизуализационных технологий распознавания и исследования патогенеза последствий и осложнений черепно-мозговой травмы. Особое внимание уделено реконструктивной и минимально инвазивной хирургии, детально описан метод компьютерного моделирования и последующего стереолитографического лазерного воспроизведения полномасштабных копий черепа, его дефектов и имплантатов,  что особенно значимо при обширных и сложных краниобазальных и краниофациальных повреждениях. Обосновано дифференцированное применение интракраниальных и эндоназальных доступов для закрытия хронических ликворных фистул. Вместе с впервые созданной в НИИ нейрохирургии им. Н.Н. Бурденко методикой эндоваскулярной реконструкции магистральных сосудов с помощью баллонов-катетеров Ф.А. Сербиненко представлены новые подходы, опирающиеся на современные технологии с использованием стентов, микроспиралей и эмболизирующих композиций.

7 GHz pulsed magnicon amplifier

The work on creation of high-power microwave amplifiers with circular deflection of an electron beam has been carried out at INP since 1967. In the latest set of experiments on 7 GHz magnicon almost all design parameters have been achieved: the output power of 55 MW, efficiency of 56 %, and gain of 72 dB. Experimental data are in an excellent agreement with simulation results obtained by the special magnicon computer codes. Achieved results allow us to consider a magnicon as an alternative power source for linear colliders applications

Physical and sports activity in the context of transformation of valuable understanding of the phenomenon of corporation under the influence of external factors

The article discusses the features of the functioning of physical culture and sports activities under the influence of external factors, when there is a transformation of the value understanding of the phenomenon of human corporality in the system of physical education. Philosophical, sociological aspects of human corporeality have been considered for more than one millennium, finding new theoretical and methodological justifications. At the same time, the positions of related sciences correlated with the theory of physical culture are not sufficiently disclosed, so in relation to the global problems of humanity today, the modern challenges of the scientific and technological phase of the era are clearly visible. Information development is accompanied by an accelerating change in the living conditions of people, and leads to the drama of modern civilization: a “dangerous” increase in the gap between the level of technological, economic development and the level of spirituality.В статье рассматриваются особенности функционирования физкультурно-спортивной деятельности в условиях влияния внешних факторов, когда происходит трансформация ценностного понимания феномена телесности человека в системе физического воспитания. Философские, социологические аспекты телесности человека рассматривались не одно тысячелетие, находя новые теоретико-методические обоснования. Вместе с тем недостаточно раскрываются позиции смежных наук, соотносимых с теорией физической культуры, так в соотношении с глобальными проблемами человечества сегодняшнего дня, отчетливо просматриваются современные вызовы научно-технологической фазы эпохи. Информационное развитие, сопровождается ускоряющимся изменением условий жизнедеятельности людей, и приводит к драме современной цивилизации: “опасное” увеличение разрыва между уровнем технологического, экономического развития и уровнем духовности