Análisis morfológico y molecular de los blenios de agua dulce: una nueva especie del género Salaria Forsskål, 1775 (Actinopterygii, Blennidae) en Marruecos.

The North African freshwater fish fauna is scarce compared to other regions of the world, probably due to historical and climatic factors. The western part of North Africa is more diverse than central or eastern areas. Populations of freshwater blennids that have been traditionally ascribed to the species Salaria fluviatilis nowadays inhabit the Sebou basin in Morocco. In this study we demonstrated morphologically and molecularly that these Moroccan populations constitute a new species, for which we provide a formal description. The following diagnostic characters distinguish the newly described species from other freshwater blennids: head without diagonal rows of dark dots or blotches on cheeks; supra-ocular tentacle thick and simple (not branched); 13-15 teeth on the upper jaw and 14-16 on the lower jaw; 16-17 soft anal fin rays; short pre-orbital distance (cephalic index: 2.9-3.8); height of the anterior (spiny) part of the dorsal fin short (dorsal fin index: 0.8-1.3) and 34 vertebrae. Mitochondrial and nuclear genetic distances between the new Salaria species and either S. economidisi or S. fluviatilis are high, especially in the mitochondrial control region (18%), while these genetic distances range from 2 to 3% when the nuclear S7 marker is compared. These morphological and molecular characters differentiate the new species from the remaining freshwater species of the genus (Salaria economidisi and S. fluviatilis). The new species is confined to a small restricted area of the Sebou basin in Morocco and should be considered endangered (EN) according to the IUCN Red List.La fauna de peces de agua dulce del norte de África es escasa comparada con otras regiones del mundo debido fundamentalmente a factores históricos y climáticos. El oeste del norte de África es más diverso que las regiones del centro y del este. En la cuenca del río Sebou habitan actualmente poblaciones de blenios de agua dulce que han sido tradicionalmente adscritas a la especie Salaria fluviatilis. En este estudio demostramos morfológica y molecularmente que estas poblaciones marroquíes constituyen una nueva especie, para la cual proveemos una descripción formal. Los siguientes caracteres diagnósticos distinguen a la nueva especie descrita del resto de especies de blenios de agua dulce: cabeza sin una fila diagonal de puntos oscuros o manchas en las mejillas; tentáculo supraocular grueso y simple (no ramificado); 13-15 dientes en la mandíbula superior y 14-16 en la inferior; 16-17 radios blandos en la aleta anal; distancia preorbital corta (índice cefálico: 2.9-3.8); altura de la region anterior de la aleta dorsal corta (índice dorsal: 0.8-1.3) y 34 vértebras. Las distancias genéticas mitocondriales y nuclear entre la nueva especie de Salaria y las otras dos especies son altas, especialmente en la región control (18%), mientras que estas distancias genéticas variaron entre 2-3% para el gen nuclear S7. Estos caracteres morfológicos y moleculares diferencian a la nueva especie del resto de blenios de agua dulce del género (Salaria fluviatilis y S. economidisi). La nueva especie está confinada a la cuenca del río Sebou en Marruecos y debería ser considerada como En Peligro (EN) de acuerdo a las categorías de la lista roja

A novel scalable representative-based forecasting approach of service quality

© 2020, Springer-Verlag GmbH Austria, part of Springer Nature. Several approaches to forecast the service quality based on its quality of service (QoS) properties are reported in the literature. However, their main disadvantage resides in their limited scalability. In fact, they elaborate a forecasting model for each quality attribute per service, which cannot scale well for large or even medium size datasets of services. Accordingly, we propose a novel scalable representative-based forecasting approach of QoS. The QoS is modeled as a multivariate time series in which the values of service attributes are evaluated at each time instant and forecasted based on three stages. First, a data aggregation function is applied to the multivariate time series data. Then, principal component analysis (PCA) is applied to the quality attributes to determine the most relevant ones. The reduced data is then clustered, so that, a representative for each cluster is computed. Finally, a forecasting model is built for each cluster representative for the sake of deriving other services’ forecasting models. A set of extensive experiments are carried out to assess the efficiency and accuracy of the proposed approach on a dataset of real services. The experimental results show that the proposed approach is up to 75% more efficient than direct forecasting approaches using time measurements while increasing the number of forecasted services and that the elaborated forecasting models enjoy insignificant forecasting errors

Pathotypic diversity of Rhynchosporium secalis (Oudem) in Tunisia

Scald, caused by Rhynchosporium secalis (Oudem), is an important disease of barley in Tunisia particularly in northern, northwestern and central parts of the country where the climate is usually cold and wet during most of the barley growing season. Pathogenic variability of the barley scald pathogen in Tunisia was determined by testing the pathogenicity of 100 isolates from 5 different regions on 19 host differentials. Pathotypic diversity was high, with 93 R. secalis pathotypes identified on two differential sets (one comprising 9 and the other 10 barley lines) containing known resistance genes. A few pathotypes comprised 2% of the isolates; however, the majorities were represented by a single isolate. None of the differential lines was resistant to all isolates. The differential cultivar “Astrix” was the least compatible with the scald pathotypes followed by the differential cultivars “Atlas” and “Abyssinia”. Compatibility of the pathotypes on “Rihane” (69%) was close to that on “Osiris” (73%) and “La Mesita” (61%). None of the pathotypes was found in all the five regions of Tunisia surveyed. Some pathotypes were specific to a single region while others were found in several regions. The incidence of pathotypes varied considerably among regions, with region 3 (northwestern Tunisia) comprising the largest number of pathotypes. Virulent pathotypes were recovered in all regions but more pathotypic variability (44%) was observed in the semi-arid region 3. Differential cultivars allowed classification of R. secalis in four virulence groups. Canonical discriminant analysis showed no apparent association between virulence and geographical origin of the populations. Pathogenic variability in R. secalis in Tunisia was found not to be associated with geographical region, hence, the necessity for deployment of different resistance sources in major barley growing areas.Key words: Rhynchosporium secalis, barley, virulence groups, pathotypic variation

Accident vasculaire cerebral ischemique post-chimiotherapie pour cancer du sein

IntroductionLa chimiothérapie anticancéreuse est pourvoyeuse d’un large spectre de complications neurologiques. Parmi ces complications les accidents vasculaires cérébraux ischémiques (AVCI) restent un évènement inhabituel dont l’incidence est de 0,14%.ObjectifRapporter et commenter l’observation d’une patiente ayant présenté un AVCI dans les suites d’une chimiothérapie pour cancer du sein.ObservationPatiente âgée de 46ans, traitée pour une épilepsie généralisée, et sans facteur de risque cardiovasculaire, est suivie pour un cancer du sein. Elle a présenté au décours de la chimiothérapie un accident vasculaire cérébral ischémique sylvien droit confirmé au scanner cérébral. Les explorations cardiaques sont normales, ainsi que le bilan biologique étiologique.DiscussionLe diagnostic d’AVC post-chimiothérapie a été retenu chez notre patiente sur la base de l’absence de facteur de risque vasculaire et la négativité du bilan étiologique, ainsi que l’usage du 5 Fluorouracile qui a été incriminé dans la survenue d’AVC ischémiques. La survenue de cette complication dépend du type de drogue utilisé, et de certaines associations thérapeutiques, et relèverait de plusieurs mécanismes pouvant être intriqués. Le bilan doit être le plus complet possible afin d’affirmer l’imputabilité de la chimiothérapie.ConclusionNotre observation souligne la sévérité des complications neurologiques de la chimiothérapie, ainsi que le dilemme thérapeutique posé, en l’absence de protocole de prise en charge codifié.Mots clés : Accident vasculaire cérébral, cancer du sein, chimiothérapie, toxicité, 5-Fluorouracil

Improved flux limits for neutrinos with energies above 1022^{22} eV from observations with the Westerbork Synthesis Radio Telescope

Particle cascades initiated by ultra-high energy (UHE) neutrinos in the lunar regolith will emit an electromagnetic pulse with a time duration of the order of nano seconds through a process known as the Askaryan effect. It has been shown that in an observing window around 150 MHz there is a maximum chance for detecting this radiation with radio telescopes commonly used in astronomy. In 50 hours of observation time with the Westerbork Synthesis Radio Telescope array we have set a new limit on the flux of neutrinos, summed over all flavors, with energies in excess of $4\times10^{22}$ eV.Comment: Submitted to Phys. Rev. Let

Two highly divergent alcohol dehydrogenases of melon exhibit fruit ripening-specific expression and distinct biochemical characteristics

Alcohol dehydrogenases (ADH) participate in the biosynthetic pathway of aroma volatiles in fruit by interconverting aldehydes to alcohols and providing substrates for the formation of esters. Two highly divergent ADH genes (15% identity at the amino acid level) of Cantaloupe Charentais melon (Cucumis melo var. Cantalupensis) have been isolated. Cm-ADH1 belongs to the medium-chain zinc-binding type of ADHs and is highly similar to all ADH genes expressed in fruit isolated so far. Cm-ADH2 belongs to the short-chain type of ADHs. The two encoded proteins are enzymatically active upon expression in yeast. Cm-ADH1 has strong preference for NAPDH as a co-factor, whereas Cm-ADH2 preferentially uses NADH. Both Cm-ADH proteins are much more active as reductases with Kms 10–20 times lower for the conversion of aldehydes to alcohols than for the dehydrogenation of alcohols to aldehydes. They both show strong preference for aliphatic aldehydes but Cm-ADH1 is capable of reducing branched aldehydes such as 3-methylbutyraldehyde, whereas Cm-ADH2 cannot. Both Cm-ADH genes are expressed specifically in fruit and up-regulated during ripening. Gene expression as well as total ADH activity are strongly inhibited in antisense ACC oxidase melons and in melon fruit treated with the ethylene antagonist 1-methylcyclopropene (1-MCP), indicating a positive regulation by ethylene. These data suggest that each of the Cm-ADH protein plays a specific role in the regulation of aroma biosynthesis in melon fruit

Experimental and modeling study of the autoignition of 1-hexene/iso-octane mixtures at low temperatures

Autoignition delay times have been measured in a rapid compression machine at Lille at temperatures after compression from 630 to 840 K, pressures from 8 to 14 bar, \Phi = 1 and for a iso octane/1 hexene mixture containing 82% iso-octane and 18% 1 hexene. Results have shown that this mixture is strongly more reactive than pure iso-octane, but less reactive than pure 1 hexene. It exhibits a classical low temperature behaviour, with the appearance of cool flame and a negative temperature coefficient region. The composition of the reactive mixture obtained after the cool flame has also been determined. A detailed kinetic model has been obtained by using the system EXGAS, developed in Nancy for the automatic generation of kinetic mechanisms, and an acceptable agreement with the experimental results has been obtained both for autoignition delay times and for the distribution of products. A flow rate analysis reveals that the crossed reactions between species coming from both reactants (like H-abstractions or combinations) are negligible in the main flow consumption of the studied hydrocarbons. The ways of formation of the main primary products observed and the most sensitive rate constants have been identified