Software-Defined Cloud Computing: Architectural Elements and Open Challenges

The variety of existing cloud services creates a challenge for service providers to enforce reasonable Software Level Agreements (SLA) stating the Quality of Service (QoS) and penalties in case QoS is not achieved. To avoid such penalties at the same time that the infrastructure operates with minimum energy and resource wastage, constant monitoring and adaptation of the infrastructure is needed. We refer to Software-Defined Cloud Computing, or simply Software-Defined Clouds (SDC), as an approach for automating the process of optimal cloud configuration by extending virtualization concept to all resources in a data center. An SDC enables easy reconfiguration and adaptation of physical resources in a cloud infrastructure, to better accommodate the demand on QoS through a software that can describe and manage various aspects comprising the cloud environment. In this paper, we present an architecture for SDCs on data centers with emphasis on mobile cloud applications. We present an evaluation, showcasing the potential of SDC in two use cases-QoS-aware bandwidth allocation and bandwidth-aware, energy-efficient VM placement-and discuss the research challenges and opportunities in this emerging area.Comment: Keynote Paper, 3rd International Conference on Advances in Computing, Communications and Informatics (ICACCI 2014), September 24-27, 2014, Delhi, Indi

Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor

Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing. Herein, we introduce two strategies to improve the editing efficiency using proximal dead sgRNA and chromatin-modulating peptides. We used enhanced prime-editing to generate Igf2 mutant mice with editing frequencies of up to 47% and observed germline transmission, no off-target effects, and a dwarf phenotype. This improved prime-editing method can be efficiently applied to cell research and to generate mouse models.This study was supported by the Chung Yang, Cha Young Sun, & Jang Hi Joo Memorial Fund. This study was also supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) of Korea (Korea Mouse Phenotyping Project, NRF-2013M3A9D5072550, NRF-2020M3A9D5A01082439, NRF2019R1A2C2087198, and NRF- 2019M3A9H1103792)

ABSORPTION LINE GRADIENTS IN THE SPECTRUM OF AN ELLIPTICAL GALAXY NGC 5864A

The archival long-slit spectra, covering the wavelength range 4050 ~ 5150 Å, have been used to investigate the radial behavior of absorption line fea-tures (G4300, Fe4383, Ca4455, Fe4531, and Hβ) of an elliptical galaxy NGC 5846A. The heliocentric recession velocity of NGC 5846 has been derived as 1949±87 km/s. Fe absorption lines of NGC 5846A show significant radial gradients with the mean slope of ∆/∆(r")=-0.863±0.202. There is also a significant radial gradient of G band with a slope of -1.109±0.098. On the other hand, no radial gradients has been detected on the Ca4455 and Hβ absorptions of NGC 5846A. A metallicity gradient, which is derived from the Fe line gradients, is similar to the abundance gradient predicted by Larson's (1975) dissipative models for the formation of elliptical galaxies. We also note that a galaxy-galaxy interaction could affect the line gradients of NGC 5864A

Isolation and Characterization of Novel Bacteriophages to Target Carbapenem-Resistant <i>Acinetobacter baumannii</i>

The spread of multidrug-resistant Acinetobacter baumannii in hospitals and nursing homes poses serious healthcare challenges. Therefore, we aimed to isolate and characterize lytic bacteriophages targeting carbapenem-resistant Acinetobacter baumannii (CRAB). Of the 21 isolated A. baumannii phages, 11 exhibited potent lytic activities against clinical isolates of CRAB. Based on host spectrum and RAPD-PCR results, 11 phages were categorized into four groups. Three phages (vB_AbaP_W8, vB_AbaSi_W9, and vB_AbaSt_W16) were further characterized owing to their antibacterial efficacy, morphology, and whole-genome sequence and were found to lyse 37.93%, 89.66%, and 37.93%, respectively, of the 29 tested CRAB isolates. The lytic spectrum of phages varied depending on the multilocus sequence type (MLST) of the CRAB isolates. The three phages contained linear double-stranded DNA genomes, with sizes of 41,326–166,741 bp and GC contents of 34.4–35.6%. Genome-wide phylogenetic analysis and single gene-based tree construction revealed no correlation among the three phages. Moreover, no genes were associated with lysogeny, antibiotic resistance, or bacterial toxins. Therefore, the three novel phages represent potential candidates for phage therapy against CRAB infections

Reduction of Specular Reflection Based on Linear Polarization Control for Fluorescence-Induced Diagnostic Evaluation

The primary goal of cancer surgery is to completely eliminate tumors. A real-time diagnostic method uses a fluorescence contrast agent and a surgical microscope to assess the status of tumor resection and the patient’s blood circulation. The biggest problem in imaging diagnostics using a microscope is the specular reflection phenomenon. While observing a lesion, the observation field may be obstructed due to specular reflection, making it difficult to obtain accurate results during the diagnostic process. Herein we propose a method to reduce specular reflection during tumor diagnosis by introducing a linearly polarized filter for a surgical microscope system. The method of angular direction adjustment of the filter ensures that only the horizontally polarized light passes through it, thereby obstructing the specular reflection. As a result of removing specular reflection, clear images were obtained at 90° and 270°. This experiment was conducted using phantoms and animals. Our results prove that the proposed method can be applied to imaging cameras used in internal medicine, surgery, and radiology for diagnosis