Caractérisation physico-chimique de l’eau des noix matures de nouveaux hybrides améliorés de cocotiers (Cocos nucifera L.) grands

Des hybrides améliorés de cocotiers grands ont été créés par la Station de recherche Marc Delorme en vue de satisfaire les préférences des grosses noix des acteurs de la filière cocotier. Cependant ils demeurent encore peu étudiés. Le présent travail évalue les caractéristiques physicochimiques de l’eau des noix matures de ces nouveaux hybrides en vue d’en proposer des voies de valorisation. Les travaux portent sur des noix âgées de treize mois (rang 25) d’hybrides de cocotiers grands GPY+ x GOA+, GPY+ x GRL+ et GRL+ x GOA+ en comparaison avec l’hybride PB121+, pris comme témoin. La masse des noix entières et de l’eau de coco ont été déterminés moins de 24 h après la récolte des noix de coco. Puis des échantillons d’eau de coco ont été prélevés et conservés à -15 °C avant leurs caractérisations. Les résultats ont montré que l’eau de coco mature est plus abondante et plus acide chez les hybrides améliorés de cocotiers grands que chez le PB121+. Parmi les trois hybrides grands améliorés, GRL+ x GOA+ fournit l’eau de coco mature la plus pourvue en cendres (0,49%). Les hybrides GPY+ x GOA+ et GPY+ x GRL+ renferment plus de sucres (29,37 et 28,09 mg/ml) et de polyphénols (58,11 et 65,49 ppm). Il ressort de ces études que l’eau des noix matures de ces hybrides pourrait être utilisée pour la production d’alcool éthylique et de vinaigre.Mots clés : Valorisation, eau de coco, caractéristiques, vinaigre, alcool, Côte d’Ivoir

Molecular characterization of intestinal protozoan parasites from children facing diarrheal disease and associated risk factors in Yamoussoukro, Côte d’Ivoire

Diarrheal diseases are very common in children under 5 years and may lead to a delay of physical and mental development. Despite this knowledge, data on diarrheal diseases and socioeconomic determinants are still scarce in Côte d’Ivoire. This study is then conducted with the objective to fill part of this gap and specifically assess link between infant diarrhea occurrence and some major socioenvironmental factors. Stool samples were collected from children less than five suffering from diarrhea at Yamoussoukro Regional Hospital in central Côte d’Ivoire. Molecular species specific diagnosis was used to detect Cryptosporidium parvum, Giardia intestinalis and Entamoeba histolytica, three major protozoan parasites which cause diarrhea. Out of 306 stool samples examined, 62.75% were detected as positive at least for one of the protozoan parasite studied. Species specific prevalence was 36.93% for C. parvum, 20.92% for G. intestinalis and 22.55% for E. histolytica. Infection was more prevalent in children whose mothers were not educated although the difference was not statistically significant. No link was found between gender and infection while sanitation infrastructures, mother and children ages and water sources were found significantly associated with diarrhea occurrence. Awareness is then needed for women on lack of hygiene rules that could lead to diarrheal diseases burden.Key words: Diarrheal diseases, children development, parasitic protozoan, molecular characterization, socioenvironmental factors

From coconut to cassava: the coconut lethal yellowing phytoplasma is worsening the threat to food security in C\uf4te d\u2019Ivoire

Background. Cassava (Manihot esculenta Crantz) is a major staple food in the developing countries whose total world production reached approximately 278 million metric tonnes in 2017. C\uf4te d'Ivoire produces around three million tonnes of cassava every year and reached 4.54 metric tonnes in 2017. It is typically consumed as 'attiek\ue9', which is currently exported on regional and international markets. Cassava crop is now threaten by the C\uf4te d'Ivoire lethal yellowing disease (CILY) first reported and associated with a phytoplasma in Grand-Lahou in 2013. CILY destroyed over 400 ha of coconut groves in smallholder coconut farms where women farmers started planting cassava as an alternative food and cash crop in coconut lands devastated by the disease. Methods. Symptoms of leaf mosaic, curling and yellowing were observed in cassava orchards intwo coconut-growing villages located in the south coastal littoral of Grand-Lahou. Leaf samples were collected from symptom-bearing and symptomless cassava plants and subjected to total DNA extraction. PCR with phytoplasma universal 16S rRNA primers, and group-specific primers for subgroup 16SrXXII-B, \u2018Candidatus Phytoplasma palmicola\u2019-related strains; and with specific primers for African/Eastern cassava mosaic viruses (ACMV, EACMV). Amplicons were purified, cloned and sequenced. Sequences were compared to those of reference in NCBI (https://www.ncbi.nlm.nih.gov) and used for phylogeny analysis of phytoplasma and virus strains, respectively. Results. Phytoplasma DNA was amplified from six out of 12 symptom-bearing samples, five of which co-amplified virus DNA. Phytoplasma sequences showed 99% identity to those of 16SrXXII-B phytoplasmas as confirmed through phylogeny analysis. One cassava plant was co-infected with ACMV, closely related to the Angola strain, while the other four showed co-infection with both the ACMV (Angola) and an EACMV strain from Madagascar. All cassava varieties were phytoplasma-begomovirus co-infected, except the Yac\ue9 variety. Conclusions. Cassava plants in Grand-Lahou orchards were found infected by CILY phytoplasma (group 16SrXXII-B) and ACMV/EACMV virus strains. Results indicate that cassava may be an alternative host for the CILY phytoplasma, which may play a role spreading and worsening CILY epidemic. Prompt actions are required while waiting for a suitable resistant coconut cultivar. Short-term solutions may include replanting cassava yards with newly developed cassava varieties that enhance plant resilience against the coconut phytoplasma and ACMV/EACMV viruses to help supporting food production and improve livelihoods of smallholder coconut farmers in Grand-Lahou

Les effets de la réouverture de l'embouchure du fleuve Comoé sur le végétation littorale lagunaire (Lagune Ebrié - Côte d'Ivoire)

The results of floristic data collected during two surveys in the eastern region of Ebrié lagoon in 1987 (before the reopening of the inlet) and in 1988 (after the reopening of the inlet) are discussed in this article. Salinity increase (from 4‰ to 30‰) as a result of the reopening in September 1987 of the channel of Comoé river, profoundly modified qualitatively and quantitatively, the plant population. Floating or fixed macrophytes have been completely destroyed. Mangrove forest being the hydrophytic belt of the lagunar rives

Evaluation in vitro de l’activité des écorces de tige de Anogeissus leiocarpus (DC) Guill. et Perr. (Combretaceae) sur des bactéries responsables de maladies courantes en Afrique et criblage phytochimique

Les bactéries sont à l’origine de réel problème de santé publique à cause de leur implication dans de nombreuses maladies. Leur résistance aux antibiotiques est devenue l’un des problèmes les plus importants dans la lutte contre les maladies infectieuses dans le monde. L’objectif de ce travail est d’évaluer l’activité antibactérienne des extraits hexanique, méthanolique 70% (v/v) et aqueux des écorces de tige de Anogeissus leiocarpus (DC) Guill. et Perr. (Combretaceae), une plante médicinale de la flore ivoirienne, contre les bactéries des genres Staphylococcus, Pseudomonas et les entérobactéries (Shigella sp., Salmonella Typhimurium, Escherichia coli et Proteus mirabilis) par la méthode de diffusion en milieu gélosé dans des cupules et la méthode de macro-dilution en milieu liquide . Les extraits aqueux et méthanolique 70% (v/v) ont été retenus pour la détermination des paramètres antibactériens (CMI et CMB), en raison de leurs activités sur l’ensemble des bactéries sélectionnées. Par ailleurs, une analyse qualitative par chromatographie sur couche mince (CCM), effectuée sur les différents extraits, a permis de détecter plusieurs groupes de composés chimiques parmi lesquels les saponosides, les tanins, les flavonoïdes, les polyphénols, les alcaloïdes et les sesquiterpènes dont des effets antibactériens sont connus. Les CMI obtenues, sont comprises entre 0,7 ± 0,0 et 12,5 ± 0,0 mg/ml. Quant aux CMB, elles varient de 3,1 ± 0,0 à 25,0 ± 0,0 mg/ml. Ce travail justifie l’utilisation traditionnelle de Anogeissus leiocarpus dans le traitement de diverses pathologies et plus particulièrement celles d’origine bactérienne.Mots clés : Antibactérienne, Anogeissus leiocarpus, flore ivoirienne, analyse qualitative

Phytochemical study and evaluation of the antiviral activity of aqueous extracts of three medicinal plants; Xylopia aethiopica, Gliricidia sepium and Ocimum gratissimum used in Cote d'Ivoire

Background: The present work is part of the exploration of new antiviral molecules to combat antimicrobial resistance. In purpose, this study determined the phytochemical analysis, cytotoxicity and antiviral activity of extracts from three Ivorian medicinal plants; Gliricidia sepium, Ocimum gratissimum and Xylopia aethiopica against poliovirus 1, a non-enveloped RNA virus. Methodology: Aqueous extract of the three plants, which were identified at the herbarium of National Floristic Center Abidjan, was done using a previously described method. The precipitation or staining technique was used to highlight the chemical groups in the three extracts while the polyphenol content of each extract was assessed by the colorimetric method. Cytotoxicity and antiviral activity tests were performed in 96-well plates. Cytotoxicity of each extract on L20B (a genetically engineered mouse cell line) was determined by observation of the cell line carpet. Antiviral activity of three extracts against poliovirus type I was determined after 72 hours using an assay that measures inhibition of the cytopathic effect on cell culture. Results: The three plant extracts contain polyterpenes, sterols and polyphenols, flavonoids, catechetical tannins, saponosides and quinones but none of the extract contains gallic tannins. With the exception of O. gratissimum, alkaloids were found in extracts from the two other plants, and extract of G. sepium was richer in polyphenol than the other two extracts. The cell carpet of L20B after 72 hours contact period with three extracts remained intact at concentrations ranging from 2 to 1000 μg/ml. The aqueous extract of G. sepium showed higher antiviral activity on poliovirus 1 (74.569%) at 2µg/ml than the extracts of O. gratissimum (45.6112%) and X. aethiopica (44.5247%) after 72 hours of incubation. Conclusion: The extract of G. sepium showed potent antiviral activity against poliovirus 1 than that of O. gratissimum and X. aethiopica. This was justified by its higher polyphenol content than the two extracts

Effect of glycerol, peanut oil and soybean lecithin contents on the properties of biodegradable film of improved cassava starches from Côte d’Ivoire

— Edible films have been successfully used in the food packaging industry for several decades. Today natural polysaccharides, including cassava starch,are increasingly being used in the production of such biodegradable edible films and food packaging. In Côte d'Ivoire, there are improved cassava varieties whose starches have not yet been tested in the production of biodegradable films. In thisstudy, the optical and mechanical properties and the water solubility of starch-based composite films of four improved cassava varieties withadded glycerol, peanut oil and soy lecithin were determined. Starchwas obtained by cold water extraction from native cassava from the varieties Bocou 1, Bocou 2, Yavo and TMS. Films preparation was made bycasting methodwithcassava, glycerol (25-30 %), peanutoil (5-10 %) and soybean lecithin (0-5 %). Increasing the glycerol content, increased L*color valueand elongationat break and decreased a*, b*, colourdifference (ΔE*ab) and tensilestrength of the composite films. Also, increasing the oil content from 5 to 10%, increased the opacity, b*, ΔE*ab, water solubility, elongationat break but decreased L*, a* and tensilestrength. Similarly, increasing the soy lecithin content from 0 to 5%, increased the opacity, L*, b* and ΔE*ab, but decreased a*, of the starch-based composite films. The results suggest an ideal formulation of 4% starch/25% glycerol/5% oil/5% soy lecithinfor a film with optimum mechanical properties with low solubility

Illegals abortions and utero-digestives lesions: retrospective study of 12 cases in the Department of Gynecology and Obstetrics at the Treichville teaching hospital (Abidjan, Cote D’ivoire)

Background: Traumatic intestinal digestive damage after abortion by endo-uterine manoeuvres are not uncommon. The purpose of this study is to describe the diagnostic, therapeutic and prognostic aspects of these lesions.Methods: This is a retrospective study of 3 years on patients with a uterine lesion associated with a digestive traumatic injury during illegal abortions endo-uterine manoeuvres.Results: 12 patients with a median age of 23, 9 are included. The clinical manifestations are not specific: impairment of the general condition 33.3%; hyperthermia 83.3% (or 10 cases); digestive disorders such as diarrhoea 25%, vomiting 33.3%; abdominal pain 100%; occlusive syndrome 16.7%; acute abdominal syndrome 75%. The seat of traumatic injuries is variable. The lesions were for hail alone in 4 cases (33.3%), colon alone for 2 cases (16.7%), rectum 1 case and epiploon 2 cases. In these 3 cases, the lesions were associated, sitting on both the hail and the colon at a time. All these lesions were associated with uterine perforation of variable siege. The therapeutic management consisted of a small bowel resection with ileostomy in 5 cases or 41.7%; colon resection with colostomy 3 cases or 25%; suture lesions after beveling beiges 5 cases either 41, 7 in 2 cases, we performed haemostasis on the bleeding epiploon. Treatment of the uterine lesion was conservative 75% of the time. The evolution on the 10 patients was favorable, 83.3%. Two patients died early in the operative course after septic shock.Conclusions: The digestive lesions are a factor aggravating the prognosis of post-abortion uterine manoeuvres. Their management must be rapid and requires close collaboration between the digestive surgeon and the Gynecologist

Interrupting seasonal transmission of Schistosoma haematobium and control of soil-transmitted helminthiasis in northern and central Côte d’Ivoire: a SCORE study protocol

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