Ribosomal protein synthesis is not regulated at the translational level in Saccharomyces cerevisiae: balanced accumulation of ribosomal proteins L16 and rp59 is mediated by turnover of excess protein.

We have investigated the mechanisms whereby equimolar quantities of ribosomal proteins accumulate and assemble into ribosomes of the yeast Saccharomyces cerevisiae. Extra copies of the cry1 or RPL16 genes encoding ribosomal proteins rp59 or L16 were introduced into yeast by transformation. Excess cry1 or RPL16 mRNA accumulated in polyribosomes in these cells and was translated at wild-type rates into rp59 or L16 proteins. These excess proteins were degraded until their levels reached those of other ribosomal proteins. Identical results were obtained when the transcription of RPL16A was rapidly induced using GAL1-RPL16A promoter fusions, including a construct in which the entire RPL16A 5\u27-noncoding region was replaced with the GAL1 leader sequence. Our results indicate that posttranscriptional expression of the cry1 and RPL16 genes is regulated by turnover of excess proteins rather than autogenous regulation of mRNA splicing or translation. The turnover of excess rp59 or L16 is not affected directly by mutations that inactivate vacuolar hydrolases

Program Repair Suggestions from Graphical State-Transition Specifications

Abstract. In software engineering, graphical formalisms, like state-transition tables and automata, are very often indispensable parts of the specifications. Such a formalism usually leads to specification re-finement that maintains the simulation/bisimulation relation between an implementation and a specification. We investigate how to use formal techniques to generate suggestions for repairing a program that breaks the bisimulation relation with a graphical specification. We use state graphs as a unified representation of the program models and specifica-tions. We propose a technique that may evaluate the cost of a repair. We present a PTIME heuristic algorithm that suggests how to repair a model state graph. We then explain how to derive repair suggestions for programs from the repair for state graphs. Finally, we report our experi-ment that checks the performance of our repair algorithms and the costs of our repairs. Key words: state graph, state transition relation, repair, graph theory, cost, evaluation, equivalence, bisimulation

Deep Wide-Field Spectrophotometry of the Open Cluster M67

We present nine color CCD intermediate-band spectrophotometry of a two square degree field centered on the old open cluster M67, from 3890$\rm \AA$ to nearly 1$\mu$. These observations are taken as a part of the BATC (Beijing-Arizona-Taipei-Connecticut) Color Survey of the Sky, for both scientific and calibration reasons. With these data we show that the BATC survey can reach its goal of obtaining spectrophotometry to a zero point accuracy of 0.01 mag, and down to V = 21 with 0.3 mag random error. We fit the color-magnitude diagrams (CMDs) with Worthey's theoretical models. The net result is the excellent fit of the 4.0 Gyr, [Fe/H] = $-0.10$ model to our data, including a good fit to the main sequence (MS) turn-off. Our data are consistent with a toy model with 50\% of the stars in M67 being binaries and a random distribution of binary mass-ratios, although other models with different mass-ratio distributions cannot be ruled out. The spatial distribution and mass function (MF) of stars in M67 show marked effects of dynamical evolution and evaporation of stars from the cluster. Blue stragglers and binary stars are the most condensed within the cluster, with degree of condensation depending on mass.We find M67 to have an elongated shape, oriented at an angle of $15^{\circ}$ relative to the galactic plane. Within its tidal radius, the observed MF of M67 between 1.2 $\rm M_\odot$ and $\rm 0.8 M_\odot$ has a Salpeter slope $\rm \eta = -1.93 \pm 0.66$. For stars of mass below 0.8 $\rm M_\odot$, $\rm \eta \sim 0$. It is plausible that the leveling-off of the MF at lower masses is a result of evaporation of lower mass stars in this mass range at a rate of one every $\sim 10^7$ years. If so, it is plausible that the IMF of M67 has the canonical field value of $\rm \eta = -2.0$.Comment: 74 pages, including 19 ps figures. Accepted for publication in AJ, Aug, 199

Simulation Subsumption in Ramsey-Based Büchi Automata Universality and Inclusion Testing

International audienc

Dichotomy in the NRT Gene Families of Dicots and Grass Species

A large proportion of the nitrate (NO3−) acquired by plants from soil is actively transported via members of the NRT families of NO3− transporters. In Arabidopsis, the NRT1 family has eight functionally characterised members and predominantly comprises low-affinity transporters; the NRT2 family contains seven members which appear to be high-affinity transporters; and there are two NRT3 (NAR2) family members which are known to participate in high-affinity transport. A modified reciprocal best hit (RBH) approach was used to identify putative orthologues of the Arabidopsis NRT genes in the four fully sequenced grass genomes (maize, rice, sorghum, Brachypodium). We also included the poplar genome in our analysis to establish whether differences between Arabidopsis and the grasses may be generally applicable to monocots and dicots. Our analysis reveals fundamental differences between Arabidopsis and the grass species in the gene number and family structure of all three families of NRT transporters. All grass species possessed additional NRT1.1 orthologues and appear to lack NRT1.6/NRT1.7 orthologues. There is significant separation in the NRT2 phylogenetic tree between NRT2 genes from dicots and grass species. This indicates that determination of function of NRT2 genes in grass species will not be possible in cereals based simply on sequence homology to functionally characterised Arabidopsis NRT2 genes and that proper functional analysis will be required. Arabidopsis has a unique NRT3.2 gene which may be a fusion of the NRT3.1 and NRT3.2 genes present in all other species examined here. This work provides a framework for future analysis of NO3− transporters and NO3− transport in grass crop species

Analysis of urinary oligosaccharides in lysosomal storage disorders by capillary high-performance anion-exchange chromatography–mass spectrometry

Many lysosomal storage diseases are characterized by an increased urinary excretion of glycoconjugates and oligosaccharides that are characteristic for the underlying enzymatic defect. Here, we have used capillary high-performance anion-exchange chromatography (HPAEC) hyphenated to mass spectrometry to analyze free oligosaccharides from urine samples of patients suffering from the lysosomal storage disorders fucosidosis, α-mannosidosis, GM1-gangliosidosis, GM2-gangliosidosis, and sialidosis. Glycan fingerprints were registered, and the patterns of accumulated oligosaccharides were found to reflect the specific blockages of the catabolic pathway. Our analytical approach allowed structural analysis of the excreted oligosaccharides and revealed several previously unpublished oligosaccharides. In conclusion, using online coupling of HPAEC with mass spectrometric detection, our study provides characteristic urinary oligosaccharide fingerprints with diagnostic potential for lysosomal storage disorders