Biological Peculiarities of Landscape Design in Cottage Gardens of the Right-Bank Part of Zaporizhzhya

The choice of architectural planning, selection of plants, small architectural forms, materials, decor and other elements of landscaping has a certain dependence on the region. Therefore, identifying certain features of the design of gardens in a particular region can be useful for landscape designers who work on that location. The article is devoted to the analysis of design solutions of 5 cottage gardens on the right bank of Zaporozhye. The study was conducted using the method of comparative analysis of master plans for landscaping and landscaping of selected cottages. It is established that all surveyed areas have a flat relief, which eliminates the need for vertical planning. Regularity prevails in the architectural planning of gardens. The planning of the surveyed estates has two directions namely, decorative and decorative-utilitarian. Features of the decorative direction are the variety of ornamental vegetation, the presence of ponds, exquisite flower beds, the use of various garden equipment and decor. For gardens of decorative and utilitarian orientation is characterized by the presence of farm buildings, gardens, fruit and berry plantations, which are formed taking into account the decorative and nutritional qualities. The common features of all surveyed cottage gardens can be considered the emphasized design style, restraint and conciseness in planning and economy due to the successful selection of unpretentious plants and equipment. Landscape designers of the region can be asked at the first stage of planning to do functional zoning of the garden; the range of vegetation to select from local species and well-adapted exotics of small size; from flower beds to choose the easiest to create and care for; reservoirs, vertical and container landscaping are limited

Collapse arrest and soliton stabilization in nonlocal nonlinear media

We investigate the properties of localized waves in systems governed by nonlocal nonlinear Schrodinger type equations. We prove rigorously by bounding the Hamiltonian that nonlocality of the nonlinearity prevents collapse in, e.g., Bose-Einstein condensates and optical Kerr media in all physical dimensions. The nonlocal nonlinear response must be symmetric, but can be of completely arbitrary shape. We use variational techniques to find the soliton solutions and illustrate the stabilizing effect of nonlocality.Comment: 4 pages with 3 figure

Ribonucleolytic activity of mycoplasmas

Mycoplasmas are incapable of de novo synthesis of nucleotides and must therefore secrete nucleases in order to replenish the pool of nucleic acid precursors. The nucleolytic activity of mycoplasmas is an important factor in their pathogenicity. Bacterial ribonucleases (RNases) may produce a broad spectrum of biological effects, including antiviral and antitumor activity. Mycoplasma RNases are therefore of interest. In the present work, the capacity of Acholeplasma laidlawii and Mycoplasma hominis for RNase synthesis and secretion was studied. During the stationary growth phase, these organisms were found to synthesize Mg2+-dependent RNases, with their highest activity detected outside the cells. Localization of A. laidlawii RNases was determined: almost 90% of the RNase activity was found to be associated with the membrane vesicles. Bioinformational analysis revealed homology between the nucleotide sequences of 14 Bacillus subtilis genes encoding the products with RNase activity and the genes of the mycoplasmas under study. Amino acid sequences of 4 A. laidlawii proteins with ribonuclease activity and the Bsn RNase were also established. © 2014 Pleiades Publishing, Ltd

Modulational instability, solitons and beam propagation in spatially nonlocal nonlinear media

We present an overview of recent advances in the understanding of optical beams in nonlinear media with a spatially nonlocal nonlinear response. We discuss the impact of nonlocality on the modulational instability of plane waves, the collapse of finite-size beams, and the formation and interaction of spatial solitons.Comment: Review article, will be published in Journal of Optics B, special issue on Optical Solitons, 6 figure

STUDY OF MINIMAL RESIDUAL DISEASE BY MULTICOLOR FLOW CYTOMETRY IN MULTIPLE MYELOMA AFTER AUTOLOGOUS HEMATOPOIETIC STEM CELL TRANSPLANTATION

The frequency of achieving complete remission, as well as overall and disease-free survival, in multiple myeloma (MM) had increased due to introduction in MM treatment regimens of high-dose chemotherapy with following autologous hematopoietic stem cell transplantation (ASCT). However the number of relapses remains high, caused by persistence of residual tumor cells, i.e., the presence of minimal residual disease (MRD). One of the methods for MRD study is multicolor flow cytometry (MFC) where abnormal expression of surface antigens on myeloma plasma cells (PC) is determined. The aim of our study was to investigate the MRD by MFC before and after ASCT, the frequency of MRD-negative status achievement in complete remission (CR) patients at +100 days after ASCT and the frequency of abnormal expressed antigens on myeloma plasma cells. The study included40 MMpatients in CR at +100 days after ASCT and showed that the most common aberrations of PC were: abnormal absence of CD19 and/or CD27, decreased expression of CD38 and abnormal presence of CD56. The proportion of myeloma PCs from all bone marrow cells decreased significantly after ASCT: 20 % of patients acquired MRD-negative status, 10 % had a decrease in the number of abnormal PCs by one fold. Analysis of probability of immunochemical relapse showed that the worst prognosis was in patients with MRD-positive status before and after ASCT. During the MRD monitoring within 3-18 months, MRD-relapses were detected with the subsequent development of immunochemical relapse. The detection MRD in the dynamics is more informative than the study at only one step of therapy. It may help to select more adequate treatment for patient with multiple myeloma in each specific case

Frontal EEG asymmetry as a possible indicator of determining psychophysiological development of infants

The article is described the attempt of using a specific electroencephalogram pattern - frontal EEG asymmetry as possible marker to identify risk groups of formation of mental disorders in children at a later age. Case-control study was in groups of full-term infants and infants born preterm. Preterm infants are strongly characterized by a «right» type of asymmetry and higher absolute values of this pattern.В статье рассмотрено использование специфического электроэнцефалографического показателя, так называемого паттерна фронтальной ЭЭГ асимметрии в качестве одного из возможных методов выделения группы риска формирования психических расстройств у детей в более старшем возрасте. Исследование типа «случай-контроль» было проведено в двух группах детей: родившихся доношенными и недоношенными. Дети, родившиеся недоношенными, характеризуются преимущественно правым типом асимметрии, а также имеют более высокие абсолютные значения данного показателя

Diagnostics and treatment challenges of Ph-like acute lymphoblastic leukemia: a description of 3 clinical cases

B-cell acute lymphoblastic leukemia (B-ALL) is a diverse group of malignant blood disorders both with regard to the biological properties of the tumor and to therapeutic approaches. Immunophenotyping, molecular genetic techniques, whole-genome sequencing characterize B-ALL as a very diverse group for sensitivity to chemotherapy and prognosis. We present three clinical cases of patients with B-ALL and expected good response to standard therapy, in whom standard protocol treatment failured: refractoriness, persistence of minimal residual disease (MRD), and progression (MRD increase). The remission in these patients was achieved after chemotherapy change to immunological targeted therapy. Nowadays a unified therapeutic approach to all primary patients of the B-ALL is considered generally outdated. Great efforts are carrying out to develop molecular genetic classifications. The molecular dissection of subtypes of B-ALL goes on, and new protocols for selective treatment with targeting are clearly outlined for each subtype of B-ALL

ЗАВИСИМОСТЬ УРОВНЯ ИНФИЦИРОВАННОСТИ САЛЬМОНЕЛЛАМИ В ПОПУЛЯЦИЯХ КУР ОТ АНТАГОНИСТИЧЕСКОЙ АКТИВНОСТИ LACTOBACILLACEAE И ENTEROCOCCACEAE В ОТНОШЕНИИ SALMONELLA ENTERICA

The antagonistic activity of lactobacilli in the intestine in relation to various enteropathogenic microorganisms can vary within wide limits, including depending on the species composition of the lactobiota of the intestine. The purpose of this work was to determine the antagonistic activity of representatives of the order Lactobacillales isolated from chickens in poultry farms with different levels of Salmonella infection. The test object was the chickens of the parent herd and broiler chickens of crosses Ross 308 and Hubbard F - 15 from five poultry farms. Three poultry farms were characterized by a low level of salmonella infection in birds (less than 5% for cloacal swabs in PCR and the absence of salmonella isolation from food products). Two poultry farms were characterized by a high level of Salmonella infection (poultry infection by cloacal swabs of more than 10% and official salmonellosis disadvantage due to isolation of Salmonella cultures in food products). The level of infection was evaluated by real-time PCR after preliminary subculture of cloacal swabs on Shadler’s broth. The antagonistic activity of lactobacilli and related bacterial species isolated from the same chickens was carried out in co-cultivation tests on the Shadler broth with subsequent identification of salmonella on the RVS broth. Poultry farms with low Salmonella infection were characterized by the presence of L. reuteri as a major component of intestinal lactobiota and had a higher antagonistic activity against more Salmonella cultures (odds ratio (OR) 17.33 (CI 95 = 5.99-50.07776))Антагонистическая активность лактобактерий в кишечнике в отношении различных энтеропатогенных микроорганизмов может варьировать в широких пределах, в т. ч. в зависимости от видового состава лактобиоты кишечника. Цель настоящей работы заключалась в определении антагонистической активности представителей отряда Lactobacillales, выделенных от кур на птицефабриках с разными уровнями инфицированности сальмонеллами. Тест-объектом являлись куры родительского стада и цыплята-бройлеры кроссов Ross 308 и Hubbard F-15 с пяти птицефабрик. Три птицефабрики характеризовались низким уровнем инфицированности птицы сальмонеллами (менее 5 % по клоакальным смывам в ПЦР и отсутствие выделения сальмонелл из пищевой продукции). Две птицефабрики отличались высоким уровнем инфицированности сальмонеллами (инфицированность птицы по клоакальным смывам более 10 % и официальное неблагополучие по сальмонеллезу ввиду выделения культур сальмонелл в пищевой продукции). Уровень инфицированности оценивали методом ПЦР в режиме реального времени после предварительного субкультивирования клоакальных смывов на бульоне Шэдлера. Антагонистическую активность лактобактерий и родственных видов бактерий, выделенных от этих же кур, проводили в тестах по сокультивировнию на бульоне Шэдлера с последующим выявлением сальмонелл на RVS-бульоне. Птицефабрики с низким уровнем инфицированности сальмонеллами характеризовались наличием L. reuteri в качестве мажорного компонента лактобиоты кишечника и обладали более высокой антагонистической активностью в отношении большего числа культур сальмонелл (отношение шансов (OR) 17,33 (СI 95= 5,990–50,077))

КЛИНИЧЕСКИЙ ОПЫТ ПРИМЕНЕНИЯ ВНУТРИПРОСВЕТНОЙ БРАХИТЕРАПИИ НА АППАРАТЕ «MULTISOURCE» У БОЛЬНЫХ РАКОМ ЛЕГКИХ И ТРАХЕИ

Treatment of primary and recurrent cancer of the trachea and main bronchi is a complex clinical problem. The article presents own experience of contact intraluminal radiation therapy in patients of this group. Efficacy and toxicity of this treatment was evaluated.Лечение первичного и рецидивного рака трахеи и главных бронхов является сложной клинической задачей. В статье представлен собственный опыт применения внутрипросветной контактной лучевой терапии улиц данной группы. Дана оценка эффективности и токсичности данного вида лечения

Co-infection and ICU-acquired infection in COIVD-19 ICU patients: a secondary analysis of the UNITE-COVID data set

Background: The COVID-19 pandemic presented major challenges for critical care facilities worldwide. Infections which develop alongside or subsequent to viral pneumonitis are a challenge under sporadic and pandemic conditions; however, data have suggested that patterns of these differ between COVID-19 and other viral pneumonitides. This secondary analysis aimed to explore patterns of co-infection and intensive care unit-acquired infections (ICU-AI) and the relationship to use of corticosteroids in a large, international cohort of critically ill COVID-19 patients.Methods: This is a multicenter, international, observational study, including adult patients with PCR-confirmed COVID-19 diagnosis admitted to ICUs at the peak of wave one of COVID-19 (February 15th to May 15th, 2020). Data collected included investigator-assessed co-infection at ICU admission, infection acquired in ICU, infection with multi-drug resistant organisms (MDRO) and antibiotic use. Frequencies were compared by Pearson's Chi-squared and continuous variables by Mann-Whitney U test. Propensity score matching for variables associated with ICU-acquired infection was undertaken using R library MatchIT using the "full" matching method.Results: Data were available from 4994 patients. Bacterial co-infection at admission was detected in 716 patients (14%), whilst 85% of patients received antibiotics at that stage. ICU-AI developed in 2715 (54%). The most common ICU-AI was bacterial pneumonia (44% of infections), whilst 9% of patients developed fungal pneumonia; 25% of infections involved MDRO. Patients developing infections in ICU had greater antimicrobial exposure than those without such infections. Incident density (ICU-AI per 1000 ICU days) was in considerable excess of reports from pre-pandemic surveillance. Corticosteroid use was heterogenous between ICUs. In univariate analysis, 58% of patients receiving corticosteroids and 43% of those not receiving steroids developed ICU-AI. Adjusting for potential confounders in the propensity-matched cohort, 71% of patients receiving corticosteroids developed ICU-AI vs 52% of those not receiving corticosteroids. Duration of corticosteroid therapy was also associated with development of ICU-AI and infection with an MDRO.Conclusions: In patients with severe COVID-19 in the first wave, co-infection at admission to ICU was relatively rare but antibiotic use was in substantial excess to that indication. ICU-AI were common and were significantly associated with use of corticosteroids