The effects of medical ozone in rat heart exposed to ischemia-reperfusion injury: Experimental study

Objective: In this study, it was intended to investigate the effects of preconditioning with medical ozone on possible reperfusion injury, in which patients undergoing the operation due to coronary artery disease. Material and Methods: 37 female mature rats were categorized into 4 groups either as sham-control (Group I), ischemia (Group II), ischemic preconditioning by oxygen (Group III) or by medical ozone (Group IV). Through left thoracotomy, rats were subjected to 25 min of ischemia on left anterior descending (LAD) coronary artery and after that, reperfusion was provided for 75 min. At the end of this period, serum sampling for creatinine-kinase (CK)-MB, Troponin-I, superoxide dismutase (SOD) and malondialdehyde (MDA) and tissue sampling for histopathological examinations were performed. The results of pathological examination were divided into 3 groups according to cellular damage. Results: Statistically, there were no significant differences between the groups in case of CK-MB levels and SOD enzymatic activity (p>0.05). Nevertheless, Troponin-I was higher in Group III and MDA was meaningfully lower in Group IV in respect to control group (p=0.005). Moreover, MDA levels were significantly lower in Group IV, when compared to Group III. With these results, no significant differences were detected between ischemia and ozone group. Although it is important to detect lower MDA levels in ozone group when compared to oxygen group, it is hard to talk about protective effects of ozone just depending on this data. In histopathological examination, medical ozone had beneficial effects on cellular protection when compared to ischemic group; but when the groups were examined, no significant differences could be found between oxygen and ozone groups. Conclusion: Although the role of medical ozone on the prevention of reperfusion injury could not be demonstrated clearly, it might have some beneficial effects after biochemical and histopathological evaluation. To be able to show these effects precisely, more studies are needed. Copyright © 2012 by Türkiye Klinikleri

A child with psoriasis, hypogammaglobulinemia, and monosomy 7-positive myelodysplastic syndrome [Miyelodisplastik sendrom, psöriazis, hipogamaglobulinemi ve monozomi 7’si olan bir Çocuk miyelodisplastik sendrom olgusu]

[No abstract available

Effect of Topically Applied Charged Particles on Healing of Colonic Anastomoses

Hypothesis: Various forms of electrical stimulation can improve wound healing in different tissues, but their application to gastrointestinal tract healing has not been investigated. We assumed that positively charged diethylaminoethyl cross-linked dextran bead (diethylaminoethyl Sephadex [DEAE-S]) particles would have a beneficial effect on the healing of colonic anastomoses. Design: Experimental animal study. Setting: Animal research laboratory of a university hospital. Animals: Forty female Wistar albino rats. Interventions: Right colonic transection and anastomosis was performed in 5 animal groups. The control group received no treatment; the placebo group, methylcellulose gel; and the DEAE-S group, DEAE-S in methyl cellulose gel applied topically around the anastomoses. The fecal peritonitis (FP) group underwent cecal ligation and perforation simultaneously with the anastomosis to cause FP; the FP + DEAE-S group also received DEAE-S applied around the anastomoses. Main Outcome Measures: After the completion of postoperative day 4, all rats were killed. Anastomotic bursting pressures and hydroxyproline concentrations in perianastomotic tissue were measured and compared. Results: Mean bursting pressures were 115.1 mm Hg in the control group, 113.6 mm Hg in the placebo group, 159.4 mm Hg in the DEAE-S group, 62.8 mm Hg in the FP group, and 121.1 mm Hg in the FP + DEAE-S group (P=.001, 1-way analysis of variance [ANOVA]). The differences between the control vs DEAE-S groups, placebo vs DEAE-S groups, and FP vs FP + DEAE-S groups were significant (P<.05, t test). Mean hydroxyproline concentrations were 5.2 mug/mg in the control group, 4.9 mug/mg in the placebo group, 5.6 mug/mg in the DEAE-S group, 4.5 mug/mg in the FP group, and 5.4 mug/mg in the FP + DEAE-S group (P=.09, 1-way ANOVA). The difference between the FP and FP + DEAE-S groups was significant (P=.04, t test). Conclusions: A positively charged particle, DEAE-S, improves healing of colonic anastomoses in healthy rats and in rats with FP. This inexpensive, nontoxic material is easily applied and deserves further evaluation in gastrointestinal tract healing.WoSScopu

Evaluation of cardiac functions by tissue doppler echocardiography in the long term follow-up of patients with childhood Hodgkin lymphoma [Çocukluk çagı Hodgkin lenfoma hastalarının uzun dönem izlemlerinde kalp işlevlerinin doku doppler ekokardiyografi yöntemi ile degerlendirilmesi]

Aim: Treatment-associated cardiomyopathy which is one of the side effects of treatment, can be asymptomatic in Hodgkin lymphoma patients. The aim of this study was to find out whether tissue Doppler echocardiography was superior to conventional echocardiography in determining potential cardiac problems in the patients diagnosed with Hodgkin lymphoma. Material and Method: A total of 17 Hodgkin lymphoma patients (12 males, 5 females), and whose treatment had been stopped for at least 4 years were being followed-up with disease-free status and without cardiac symptoms and a control group of 14 healthy persons (8 males, 6 females) were included in the study. The cardiac functions of the patients were evaluated by M-mode, 2 dimentional, colour Doppler, CW Doppler and pulse Doppler techniques. Data analyzes were evaluated by program of Statistical Package for Social Science for Windows 11.5, and Shapiro Wilk, Student's t testi, Mann Whitney U, Fisher's exact chi square tests, respectively. Hospital ethic committee consent was received for the study (07.05.2007/no:5639). Results: There were no statistical differences between the two groups (p=0.302 and p=0.860 respectively) when both groups were evaluated in terms of ejection fraction and fraction shortening by conventional echocardiography technique. Evaluation of both groups for the left ventricle isovolumic contraction time revealed a statistically significant longer time in the patient group compared with the control group (p: 0.038). The results were found longer in the patient group compared with the control group when both groups were evaluated in terms of myocardial performance index parameters and the left ventricle isovolumic contraction time parameters (p=0.029 and p=0.049, respectively). Conclusions: We concluded that the left ventricle isovolumic contraction time, the left ventricle isovolumic acceleration time and myocardial performance index tests are important parameters for the early detection of cardiac pathologies that may develop in relation to chemotherapy

Economic and psychosocial problems experienced by pediatric with cancer patients and their families during the treatment and follow-up process [Çocukluk çagi kanser hastalari ile ailelerinin tedavi ve izlem sürecinde yasadigi ekonomik ve psikososyal sorunlar]

Aim: To identify the psychosocial and economic problems of the pediatric patients with cancer who were treated at the Dr. Sami Ulus Obstetrics and Gynecology and Child Health and Diseases Training and Research Hospital’s Pediatric Oncology Department and their relatives during this process. Material and Methods: We interviewed a total of 100 patients who were treated at Dr. Sami Ulus Obstetrics and Gynecology and Child Health and Diseases Hospital’s Pediatric Oncology Department between 1996 and 2015, and were now followed up without treatment and their relatives using survey questions on the psychosocial and economic problems they experienced. Results: Most of patients were from provinces outside Ankara. The average monthly income was below the level that would meet the family needs in 80% of the families and only 16% had extra income. Additional economic support had been received by 93% of the families in the patient group. Twenty-five families (25%) had been forced to sell property during the treatment. Forty-nine (49%) families had borrowed money from acquaintances and relatives or had taken out a bank loan. Serious psychological problems were experienced during and after the treatment by 46% of the mothers; 41% of the families had used religious procedures more commonly during the treatment period to cope with the psychological problems. Education was subject to a 1-2 year pause in 83% of the patients. Conclusion: Childhood-age patients with cancer and their families experience significant psychosocial and economic problems during and after the treatment process. Providing medical treatment and psychosocial support in harmony is an important factor that increases the success of cancer treatment. The patient and the family will require psychosocial support mechanisms throughout life, starting from the moment they face the disease. Developing national social support programs and legal regulations to form a basis for such programs are required in our country. © 2019 by Turkish Pediatric Association

Oropharyngeal tularemia mimicking tumoral relapse in a patient with Hodgkin lymphoma in remission

Tularemia is a zoonotic disease caused by Francisella tularensis. The clinical forms mostly depend on the port of entry into humans. Ingestion typically results in the oropharyngeal form and is associated with symptoms such as fever, pharyngitis, cervical lymphadenitis, and suppuration. In this report, we describe a child treated for Hodgkin's disease presenting six years later with a left cervical lymphadenopathy mimicking a relapse

Serum Potassium-Lowering Effects of Insulin Plus Dextrose and Adrenalin Treatment that Enhance Intracellular Potassium Transitions in Hyperkalemic Diarrheic Calves

The study examined the serum potassium-lowering effects of different treatment options for hyperkalemia in diarrheic calves (n=18). Calves were allocated into three treatment groups. All groups received isotonic sodium bicarbonate solution for a period of 90 minutes. The animals in group 1 received no further treatment, those in group 2 received a further dose of 50% dextrose and insulin, and those in group 3 received a further dose of adrenaline. High serum K levels in all groups significantly decreased after treatment (AT) than before treatment (BT). Serum potassium levels AT in groups 2 and 3 were found to be significantly lower than those in group 1. Increase in pH, bicarbonate and total carbon dioxide values were observed AT in all groups. However, bicarbonate and total carbon dioxide levels in the AT were not statistically significantly different from those in the adrenaline group compared with the groups 1. These parameters were significantly lower in the group 2. Moreover, the concentration of glucose in the groups 2 in contrast to the adrenalin group was higher than that in both the BT and group 1 AT. The insulin + dextrose combination and adrenaline applications in hyperkalemic calves had similar effects. However, the administration of adrenaline had a lower negative effect on metabolic acidosis treatment. (C) 2015 PVJ. All rights reservedYuzuncu Yil University Directorate of Scientific Research ProjectsYuzuncu Yil University [2007-VF-B05]This study is part of the project supported by Yuzuncu Yil University Directorate of Scientific Research Projects (Project No: 2007-VF-B05), and we thank them for their support

Clinical and epidemiological characteristics of children with germ cell tumors: A single center experience in a developing country

Germ cell tumor (GCT) is a rare malignancy accounting for 2-3% of all pediatric tumors. The overall survival rate of children and adolescents with GCT is more than 80% after adopting combined therapy. The aim of this study is to review clinical presentation, management, and outcome in a single-center series with extracranial GCT. Clinical characteristics, pathologic presentations, and survival outcomes of 101 children with GCT, treated at our hospital from 1988 to 2011, were analyzed. Sixty-two of patients were female and 39 of them were male. Fifty-eight (57%) patients had gonadal tumor (24 testicular, 34 ovarian), 43 (43%) extragonadal. Histologically, teratomas were found most frequently (26 mature, 10 immature), followed by yolk sac tumors (n: 33), mixed malignant tumors (n: 13), embryonal carcinoma (n: 10), disgerminoma (n: 8) and seminoma (n: 1). Twenty-six patients were diagnosed as mature teratoma and we excluded them in the evaluation of staging and survival. Five-year overall and relaps-free survival were 80.3% (mean follow-up time: 215.8 months) and 73.4% (mean follow-up time: 176.2 months), respectively. Five-year survival rates were 93.2% and 90.2% in malign GCTs diagnosed after 1999. © 2017, Turkish Journal of Pediatrics. All rights reserved