The contamination of Vibrio spp. of seashell products in 5 cities of Guangxi in 2017

Objective To determine the contamination of Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio alginolyticus and Vibrio cholera in seashell products. Methods Seashell samples were collected from three coastal cities and two inland cities of Guangxi in 2017. Results There were 800 samples were collected. The total positive rate of Vibrio spp. was 76.5% (612/800). The positive rate of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholera were 73.9% (591/800), 18.4% (147/800) and 0.1% (1/800), respectively. There was no Vibrio alginolyticus detected. For Vibrio parahaemolyticus, the positive rate was related to the samples source, samples status and the species of seashell. The positive rate in coastal areas was higher than inland areas, while the quantity was lower. Both the positive rate and quantity of Vibrio spp. in the live products were higher than fresh/chilled products. The positive rate of razor fish, mud clam, oyster and short necked clam were the highest and all above 75.0%. The positive rate of scallop and mussel was relatively low but the quantity was the highest. Around 1.0% (6/591) of the Vibrio parahaemolyticus positive samples was detected virulence genes. For the Vibrio vulnificus, the positive rate in rural areas was higher than urban areas, and coastal areas was higher than inland areas. The positive rate of razor fish and mud clam was the highest and both over 35.0%. Conclusion Vibrio parahaemolyticus and Vibrio vulnificus were highly contaminated in seashell products in Guangxi. It is necessary to strengthen the health education of food safety and the surveillance of Vibrio vulnificus in coastal rural areas

Analysis of monitoring results of foodborne pathogens in infant food on the market of Guangxi Zhuang Autonomous Region in 2011-2016

Objective To understand the contamination status and trend of foodborne pathogens in infant food in Guangxi Zhuang Autonomous Region, and provide reference to prevent and control the foodborne disease effectively. Methods Collecting infant food from 14 cities of Guangxi Zhuang Autonomous Region in 2011-2016. Four kinds of pathogenic bacteria were tested according to the food safety standard microbiological test (GB 4789) and the national food pollution and hazardous factors risk monitoring manual (2014). Results A total of 7 956 samples were detected. The positive rate of supplementary cereal food for infants and young children was 6.1% (249/4 084), and that of infant formula was 4.7% (183/3 872). The positive rates of Enterobacter sakazakii, Bacillus cereus (not in 2011 and 2015) and Staphylococcus aureus were 2.7%-5.6%, 2.3%-8.5% and 0.0%-0.4% respectively in supplementary cereal food for infants and young children. In the infant formula, the positive rate of Enterobacter sakazakii was from 0.2% to 1.8%. Bacillus cereus was 10.2%-12.4% (not in 2011 and 2015), and Staphylococcus aureus was 0.0%-0.3%. Salmonella was detected in one sample, and the origin was Inner Mongolia Autonomous Region. The positive rate of supplementary cereal food for infants and young children was 6.1% (248/4 077) in domestic products, while there was 1 positive in 7 foreign samples. In formula food, the positive rate was 4.7% (177/3 758) in domestic products and 5.3% (6/114) in imported products. Conclusion The contamination of Enterobacter sakazakii and Bacillus cereus in infant food sold in Guangxi Zhuang Autonomous Region posed a threat to infants. Therefore, it was necessary to strengthen the health supervision of domestic production enterprises

Potential drug-drug interaction of olverembatinib (HQP1351) using physiologically based pharmacokinetic models

Olverembatinib (HQP1351) is a third-generation BCR-ABL tyrosine kinase inhibitor for the treatment of chronic myeloid leukemia (CML) (including T315I-mutant disease), exhibits drug-drug interaction (DDI) potential through cytochrome P450 (CYP) enzymes CYP3A4, CYP2C9, CYP2C19, CYP1A2, and CYP2B6. A physiologically-based pharmacokinetic (PBPK) model was constructed based on physicochemical and in vitro parameters, as well as clinical data to predict 1) potential DDIs between olverembatinib and CYP3A4 and CYP2C9 inhibitors or inducers 2), effects of olverembatinib on the exposure of CYP1A2, CYP2B6, CYP2C9, CYP2C19, and CYP3A4 substrates, and 3) pharmacokinetics in patients with liver function injury. The PBPK model successfully described observed plasma concentrations of olverembatinib from healthy subjects and patients with CML after a single administration, and predicted olverembatinib exposure increases when co-administered with itraconazole (strong CYP3A4 inhibitor) and decreases with rifampicin (strong CYP3A4 inducer), which were validated by observed data. The predicted results suggest that 1) strong, moderate, and mild CYP3A4 inhibitors (which have some overlap with CYP2C9 inhibitors) may increase olverembatinib exposure by approximately 2.39-, 1.80- to 2.39-, and 1.08-fold, respectively; strong, and moderate CYP3A4 inducers may decrease olverembatinib exposure by approximately 0.29-, and 0.35- to 0.56-fold, respectively 2); olverembatinib, as a “perpetrator,” would have no or limited impact on CYP1A2, CYP2B6, CYP2C9, CYP2C19, and CYP3A4 enzyme activity 3); systemic exposure of olverembatinib in liver function injury with Child-Pugh A, B, C may increase by 1.22-, 1.79-, and 2.13-fold, respectively. These simulations inform DDI risk for olverembatinib as either a “victim” or “perpetrator”

Facile Synthesis of Uniform Virus-like Mesoporous Silica Nanoparticles for Enhanced Cellular Internalization

The low-efficiency cellular uptake property of current nanoparticles greatly restricts their application in the biomedical field. Herein, we demonstrate that novel virus-like mesoporous silica nanoparticles can easily be synthesized, showing greatly superior cellular uptake property. The unique virus-like mesoporous silica nanoparticles with a spiky tubular rough surface have been successfully synthesized via a novel single-micelle epitaxial growth approach in a low-concentration-surfactant oil/water biphase system. The virus-like nanoparticles' rough surface morphology results mainly from the mesoporous silica nanotubes spontaneously grown via an epitaxial growth process. The obtained nanoparticles show uniform particle size and excellent monodispersity. The structural parameters of the nanoparticles can be well tuned with controllable core diameter (60-160 nm), tubular length (6-70 nm), and outer diameter (6-10 nm). Thanks to the biomimetic morphology, the virus-like nanoparticles show greatly superior cellular uptake property (invading living cells in large quantities within few minutes, <5 min), unique internalization pathways, and extended blood circulation duration (t1/2 = 2.16 h), which is much longer than that of conventional mesoporous silica nanoparticles (0.45 h). Furthermore, our epitaxial growth strategy can be applied to fabricate various virus-like mesoporous core-shell structures, paving the way toward designed synthesis of virus-like nanocomposites for biomedicine applications. 2017 American Chemical Society.The work was supported by China National Key Basic Research Program (973 Project) (Nos. 2013CB934100 and 2012CB224805), NSFC (Grant Nos. 21322508 and 21210004), Shanghai Shuguang Program, China Postdoctoral Science Foundation (2015M570327). The authors extend their appreciation to the International Scientific Partnership Program ISPP at King Saud University for funding this research work through ISPP# 0018.Scopu

The deubiquitinase USP6 affects memory and synaptic plasticity through modulating NMDA receptor stability

人类与其他动物相比的重要区别在于人类拥有高等认知能力，这种能力集中体现在学习记忆和语言表达方面。厦门大学医学院神经科学研究所王鑫教授团队发现人科动物特异性基因USP6作为一个新的NMDA受体调控因子，可通过去泛素化途径调节NMDA型谷氨酸受体的降解和稳定性，进而调控突触可塑性和学习记忆能力。 本研究工作由王鑫教授指导完成，博士生曾凡伟、马学海与硕士生朱琳为共同第一作者，王鑫教授为通讯作者。Ubiquitin-specific protease (USP) 6 is a hominoid deubiquitinating enzyme previously implicated in intellectual disability and autism spectrum disorder. Although these findings link USP6 to higher brain function, potential roles for USP6 in cognition have not been investigated. Here, we report that USP6 is highly expressed in induced human neurons and that neuron-specific expression of USP6 enhances learning and memory in a transgenic mouse model. Similarly, USP6 expression regulates N-methyl-D-aspartate-type glutamate receptor (NMDAR)-dependent long-term potentiation and long-term depression in USP6 transgenic mouse hippocampi. Proteomic characterization of transgenic USP6 mouse cortex reveals attenuated NMDAR ubiquitination, with concomitant elevation in NMDAR expression, stability, and cell surface distribution with USP6 overexpression. USP6 positively modulates GluN1 expression in transfected cells, and USP6 down-regulation impedes focal GluN1 distribution at postsynaptic densities and impairs synaptic function in neurons derived from human embryonic stem cells. Together, these results indicate that USP6 enhances NMDAR stability to promote synaptic function and cognition.This work was partially supported by the National Natural Science Foundation of China (31871077, 81822014, 81571176 to XW; 81701349 to Hongfeng Z.; 81701130 to QZ; and 81471160 to HS), the National Key R&D Program of China (2016YFC1305900 to XW and HS), the Natural Science Foundation of Fujian Province of China (2017J06021 to XW), the Fundamental Research Funds for the Chinese Central Universities (20720150061 to XW and 20720180040 to ZS), Open Research Fund of State Key Laboratory of Cellular Stress Biology, Xiamen University (SKLCSB2019KF012 to QZ), and China Postdoctoral Science Foundation (2017M612130 to QZ).该研究得到了国家自然科学基金面上项目和优秀青年基金项目的支持

Development and validation of an ultra?performance liquid chromatography quadrupole time of flight mass spectrometry method for rapid quantification of free amino acids in human urine

An ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-qTOFMS)method using hydrophilic interaction liquid chromatography was developed and validated for simultaneous quantification of 18 free amino acids in urine with a total acquisition time including the column re-equilibration of less than 18 min per sample. This method involves simple sample preparation steps which consisted of 15 times dilution with acetonitrile to give a final composition of 25 % aqueous and 75 % acetonitrile without the need of any derivatization. The dynamic range for our calibration curve is approximately two orders of magnitude (120-fold from the lowest calibration curve point) with good linearity (r2 ? 0.995 for all amino acids). Good separation of all amino acids as well as good intra- and inter-day accuracy (<15 %) and precision (<15 %) were observed using three quality control samples at a concentration of low, medium and high range of the calibration curve. The limits of detection (LOD) and lower limit of quantification of our method were ranging from approximately 1–300 nM and 0.01–0.5 µM, respectively. The stability of amino acids in the prepared urine samples was found to be stable for 72 h at 4 °C, after one freeze thaw cycle and for up to 4 weeks at ?80 °C. We have applied this method to quantify the content of 18 free amino acids in 646 urine samples from a dietary intervention study. We were able to quantify all 18 free amino acids in these urine samples, if they were present at a level above the LOD. We found our method to be reproducible (accuracy and precision were typically <10 % for QCL, QCM and QCH) and the relatively high sample throughput nature of this method potentially makes it a suitable alternative for the analysis of urine samples in clinical setting

Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking

The potential of the diverse chemistries present in natural products (NP) for biotechnology and medicine remains untapped because NP databases are not searchable with raw data and the NP community has no way to share data other than in published papers. Although mass spectrometry techniques are well-suited to high-throughput characterization of natural products, there is a pressing need for an infrastructure to enable sharing and curation of data. We present Global Natural Products Social molecular networking (GNPS, http://gnps.ucsd.edu), an open-access knowledge base for community wide organization and sharing of raw, processed or identified tandem mass (MS/MS) spectrometry data. In GNPS crowdsourced curation of freely available community-wide reference MS libraries will underpin improved annotations. Data-driven social-networking should facilitate identification of spectra and foster collaborations. We also introduce the concept of ‘living data’ through continuous reanalysis of deposited data

Different Adsorption Behaviors and Mechanisms of Anionic Azo Dyes on Polydopamine–Polyethyleneimine Modified Thermoplastic Polyurethane Nanofiber Membranes

Considering the notable mechanical properties of thermoplastic polyurethane (TPU), polydopamine–polyethyleneimine (PEI) -modified TPU nanofiber membranes (PDA/PEI-TPU NFMs) have been developed successfully for removal of anionic azo dyes. The adsorption capacity of PDA/PEI-TPU NFMs was evaluated using three anionic dyes: congo red (CR), sunset yellow (SY), and methyl orange (MO). Interestingly, it exhibited different adsorption behaviors and mechanisms of CR on PDA/PEI-TPU NFMs compared with SY and MO. With the decrease in pH, leading to more positive charges on the PDA/PEI-TPU NFMs, the adsorption capacity of SY and MO increased, indicating electrostatic interaction as a main mechanism for SY and MO adsorption. However, wide pH range adaptability and superior adsorption have been observed during the CR adsorption process compared to SY and MO, suggesting a synergistic effect of hydrogen bonding and electrostatic interaction, likely as a critical factor. The adsorption kinetics revealed that chemical interactions predominate in the CR adsorption process, and multiple stages control the adsorption process at the same time. According to the Langmuir model, the maximum adsorption capacity of CR, SY and MO were reached 263, 17 and 23 mg/g, respectively. After six iterations of adsorption–desorption, the adsorption performance of the PDA/PEI-TPU NFMs did not decrease significantly, which indicated that the PDA/PEI-TPU NFMs have a potential application for the removal of CR molecules by adsorption from wastewater

Magnetic Technologies and Green Solvents in Extraction and Separation of Bioactive Molecules Together with Biochemical Objects: Current Opportunities and Challenges

Currently, magnetic technology and green solvents are widely used in chemical engineering, environmental engineering and other fields as they are environmentally friendly, easy to operate and highly efficient. Moreover, a magnetic field has positive effect on many physicochemical processes. However, related new methods, materials, strategies and applications in separation science still need to be developed. In this review, a series of meaningful explorations of magnetic technologies for the separation of natural products and biologic objects, including magnetic ionic liquids and other magnetic solvents and fluids, magnetic nanoparticles and magnetic fields, and the development of magnetic separators were reviewed. Furthermore, the difficulties in the application and development of magnetic separation technology were discussed on the basis of comparison and data analysis, especially for the selection of magnetic materials and magnetic field sources. Finally, the progress in the development of magnetic separators was also elaborated for researchers, mainly including that of the new high-efficiency magnetic separator through multi-technology integration and the optimization of traditional magnetic separators, which help current techniques break through their bottleneck as a powerful driving force

Microfiltration Membranes for the Removal of Bisphenol A from Aqueous Solution: Adsorption Behavior and Mechanism

This study mainly investigated the adsorption behavior and mechanism of microfiltration membranes (MFMs) with different physiochemical properties (polyamide (PA), polyvinylidene fluoride (PVDF), nitrocellulose (NC), and polytetrafluoroethylene (PTFE)) for bisphenol A (BPA). According to the adsorption isotherm and kinetic, the maximum adsorption capacity of these MFMs was PA (161.29 mg/g) > PVDF (80.00 mg/g) > NC (18.02 mg/g) > PTFE (1.56 mg/g), and the adsorption rate was PVDF (K1 = 2.373 h−1) > PA (K1 = 1.739 h−1) > NC (K1 = 1.086 h−1). The site energy distribution analysis showed that PA MFMs had the greatest adsorption sites, followed by PVDF and NC MFMs. The study of the adsorption mechanism suggested that the hydrophilic microdomain and hydrophobic microdomain had a micro-separation for PA and PVDF, which resulted in a higher adsorption capacity of PA and PVDF MFMs. The hydrophilic microdomain providing hydrogen bonding sites and the hydrophobic microdomain providing hydrophobic interaction, play a synergetic role in improving the BPA adsorption. Due to the hydrogen bonding force being greater than the hydrophobic force, more hydrogen bonding sites on the hydrophobic surface resulted in a higher adsorption capacity, but the hydrophobic interaction contributed to improving the adsorption rate. Therefore, the distribution of the hydrophilic microdomain and hydrophobic microdomain on MFMs can influence the adsorption capacity and the adsorption rate for BPA or its analogues. These consequences provide a novel insight for better understanding the adsorption behavior and mechanism on MFMs