Phytochrome Mediated Responses in Agrobacterium fabrum: Growth, Motility and Plant Infection

The soil bacterium and plant pathogen Agrobacterium fabrum C58 has two phytochrome photoreceptors, Agp1 and Agp2. We found that plant infection and tumor induction by A. fabrum is down-regulated by light and that phytochrome knockout mutants of A. fabrum have diminished infection rates. The regulation pattern of infection matches with that of bacterial conjugation reported earlier, suggesting similar regulatory mechanisms. In the regulation of conjugation and plant infection, phytochromes are active in darkness. This is a major difference to plant phytochromes, which are typically active after irradiation. We also found that propagation and motility were affected in agp1− and agp2− knockout mutants, although propagation was not always affected by light. The regulatory patterns can partially but not completely be explained by modulated histidine kinase activities of Agp1 and Agp2. In a mass spectrometry-based proteomic study, 24 proteins were different between light and dark grown A. fabrum, whereas 382 proteins differed between wild type and phytochrome knockout mutants, pointing again to light independent roles of Agp1 and Agp2

The phylogeny of Galerucinae (Coleoptera: Chrysomelidae) and the performance of mitochondrial genomes in phylogenetic inference compared to nuclear rRNA genes

With efficient sequencing techniques, full mitochondrial genomes are rapidly replacing other widely used markers, such as the nuclear rRNA genes, for phylogenetic analysis but their power to resolve deep levels of the tree remains controversial. We studied phylogenetic relationships of leaf beetles (Chrysomelidae) in the tribes Galerucini and Alticini (root worms and flea beetles) based on full mitochondrial genomes (103 newly sequenced), and compared their performance to the widely sequenced nuclear rRNA genes (full 18S, partial 28S). Our results show that: (i) the mitogenome is phylogenetically informative from subtribe to family level, and the per-nucleotide contribution to nodal support is higher than that of rRNA genes, (ii) the Galerucini and Alticini are reciprocally monophyletic sister groups, if the classification is adjusted to accommodate several ‘problematic genera’ that do not fit the dichotomy of lineages based on the presence (Alticini) or absence (Galerucini) of the jumping apparatus, and (iii) the phylogenetic results suggest a new classification system of Galerucini with eight subtribes: Oidina, Galerucina, Hylaspina, Metacyclina, Luperina, Aulacophorina, Diabroticina and Monoleptina

On the existence and uniqueness of solutions to stochastic differential equations driven by G-Brownian motion with integral-Lipschitz coefficients

In this paper, we study the existence and uniqueness of solutions to stochastic differential equations driven by G-Brownian motion (GSDEs) with integral-Lipschitz conditions on their coefficients

The Disk Substructures at High Angular Resolution Project (DSHARP). IV. Characterizing Substructures and Interactions in Disks around Multiple Star Systems

To characterize the substructures induced in protoplanetary disks by the interaction between stars in multiple systems, we study the 1.25 mm continuum and the 12CO(J = 2–1) spectral line emission of the triple systems HT Lup and AS 205, at scales of ≈5 au, as part of the Disk Substructures at High Angular Resolution Project (DSHARP). In the continuum emission, we find two symmetric spiral arms in the disk around AS 205 N, with a pitch angle of 14°, while the southern component AS 205 S, itself a spectroscopic binary, is surrounded by a compact inner disk and a bright ring at a radius of 34 au. The 12CO line exhibits clear signatures of tidal interactions, with spiral arms, extended arc-like emission, and high velocity gas, possible evidence of a recent close encounter between the disks in the AS 205 system, as these features are predicted by hydrodynamic simulations of flyby encounters. In the HT Lup system, we detect continuum emission from all three components. The primary disk, HT Lup A, also shows a two-armed symmetric spiral structure with a pitch angle of 4°, while HT Lup B and C, located at 25 and 434 au in projected separation from HT Lup A, are barely resolved with ~5 and ~10 au in diameter, respectively. The gas kinematics for the closest pair indicates a different sense of rotation for each disk, which could be explained by either a counter rotation of the two disks in different, close to parallel, planes, or by a projection effect of these disks with a close to 90° misalignment between them

The Disk Substructures at High Angular Resolution Project (DSHARP). VI. Dust Trapping in Thin-ringed Protoplanetary Disks

A large fraction of the protoplanetary disks observed with ALMA display multiple well-defined and nearly perfectly circular rings in the continuum, in many cases with substantial peak-to-valley contrast. The DSHARP campaign shows that several of these rings are very narrow in radial extent. In this Letter we test the hypothesis that these dust rings are caused by dust trapping in radial pressure bumps, and if confirmed, put constraints on the physics of the dust trapping mechanism. We model this process analytically in 1D, assuming axisymmetry. By comparing this model to the data, we find that all rings are consistent with dust trapping. Based on a plausible model of the dust temperature we find that several rings are narrower than the pressure scale height, providing strong evidence for dust trapping. The rings have peak absorption optical depth in the range between 0.2 and 0.5. The dust masses stored in each of these rings is of the order of tens of Earth masses, though much ambiguity remains due to the uncertainty of the dust opacities. The dust rings are dense enough to potentially trigger the streaming instability, but our analysis cannot give proof of this mechanism actually operating. Our results show, however, that the combination of very low and very large grains can be excluded by the data for all the rings studied in this Letter