671 research outputs found
Emerging Strategies in TCR-Engineered T Cells
Immunotherapy of cancer has made tremendous progress in recent years, as demonstrated by the remarkable clinical responses obtained from adoptive cell transfer (ACT) of patient-derived tumor infiltrating lymphocytes, chimeric antigen receptor (CAR)-modified T cells (CAR-T) and T cell receptor (TCR)-engineered T cells (TCR-T). TCR-T uses specific TCRS optimized for tumor engagement and can recognize epitopes derived from both cell-surface and intracellular targets, including tumor-associated antigens, cancer germline antigens, viral oncoproteins, and tumor-specific neoantigens (neoAgs) that are largely sequestered in the cytoplasm and nucleus of tumor cells. Moreover, as TCRS are naturally developed for sensitive antigen detection, they are able to recognize epitopes at far lower concentrations than required for CAR-T activation. Therefore, TCR-T holds great promise for the treatment of human cancers. In this focused review, we summarize basic, translational, and clinical insights into the challenges and opportunities of TCR-T. We review emerging strategies used in current ACT, point out limitations, and propose possible solutions. We highlight the importance of targeting tumor-specific neoAgs and outline a strategy of combining neoAg vaccines, checkpoint blockade therapy, and adoptive transfer of neoAg-specific TCR-T to produce a truly tumor-specific therapy, which is able to penetrate into solid tumors and resist the immunosuppressive tumor microenvironment. We believe such a combination approach should lead to a significant improvement in cancer immunotherapies, especially for solid tumors, and may provide a general strategy for the eradication of multiple cancers
Earthquake Forecast via Neutrino Tomography
We discuss the possibility of forecasting earthquakes by means of
(anti)neutrino tomography. Antineutrinos emitted from reactors are used as a
probe. As the antineutrinos traverse through a region prone to earthquakes,
observable variations in the matter effect on the antineutrino oscillation
would provide a tomography of the vicinity of the region. In this preliminary
work, we adopt a simplified model for the geometrical profile and matter
density in a fault zone. We calculate the survival probability of electron
antineutrinos for cases without and with an anomalous accumulation of electrons
which can be considered as a clear signal of the coming earthquake, at the
geological region with a fault zone, and find that the variation may reach as
much as 3% for emitted from a reactor. The case for a beam
from a neutrino factory is also investigated, and it is noted that, because of
the typically high energy associated with such neutrinos, the oscillation
length is too large and the resultant variation is not practically observable.
Our conclusion is that with the present reactor facilities and detection
techniques, it is still a difficult task to make an earthquake forecast using
such a scheme, though it seems to be possible from a theoretical point of view
while ignoring some uncertainties. However, with the development of the
geology, especially the knowledge about the fault zone, and with the
improvement of the detection techniques, etc., there is hope that a medium-term
earthquake forecast would be feasible.Comment: 6 pages, 4 figures, 1 tabl
Monoclonal T-Cell Receptors: New Reagents for Cancer Therapy
Adoptive transfer of antigen-specific T lymphocytes is an effective form of immunotherapy for persistent virus infections and cancer. A major limitation of adoptive therapy is the inability to isolate antigen-specific T lymphocytes reproducibly. The demonstration that cloned T-cell receptor (TCR) genes can be used to produce T lymphocyte populations of desired specificity offers new opportunities for antigen-specific T-cell therapy. TCR gene-modified lymphocytes display antigen-specific function in vitro, and were shown to protect against virus infection and tumor growth in animal models. A recent trial in humans demonstrated that TCR gene-modified T cells persisted in all and reduced melanoma burden in 2/15 patients. In future trials, it may be possible to use TCR gene transfer to equip helper and cytotoxic T cells with new antigen-specificity, allowing both T-cell subsets to cooperate in achieving improved clinical responses. Sequence modifications of TCR genes are being explored to enhance TCR surface expression, while minimizing the risk of pairing between introduced and endogenous TCR chains. Current T-cell transduction protocols that trigger T-cell differentiation need to be modified to generate âundifferentiatedâ T cells, which, upon adoptive transfer, display improved in vivo expansion and survival. Both, expression of only the introduced TCR chains and the production of naĂŻve T cells may be possible in the future by TCR gene transfer into stem cells
The Berry-Esseen bounds of wavelet estimator for regression model whose errors form a linear process with a Ï-mixing
Metabolomics demonstrates divergent responses of two Eucalyptus species to water stress
Past studies of water stress in Eucalyptus spp. generally highlighted the role of fewer than five âimportantâ metabolites, whereas recent metabolomic studies on other genera have shown tens of compounds are affected. There are currently no metabolite profiling data for responses of stress-tolerant species to water stress. We used GCâMS metabolite profiling to examine the response of leaf metabolites to a long (2 month) and severe (Κpredawn < â2 MPa) water stress in two species of the perennial tree genus Eucalyptus (the mesic Eucalyptus pauciflora and the semi-arid Eucalyptus dumosa). Polar metabolites in leaves were analysed by GCâMS and inorganic ions by capillary electrophoresis. Pressureâvolume curves and metabolite measurements showed that water stress led to more negative osmotic potential and increased total osmotically active solutes in leaves of both species. Water stress affected around 30â40% of measured metabolites in E. dumosa and 10â15% in E. pauciflora. There were many metabolites that were affected in E. dumosa but not E. pauciflora, and some that had opposite responses in the two species. For example, in E. dumosa there were increases in five acyclic sugar alcohols and four low-abundance carbohydrates that were unaffected by water stress in E. pauciflora. Re-watering increased osmotic potential and decreased total osmotically active solutes in E. pauciflora, whereas in E. dumosa re-watering led to further decreases in osmotic potential and increases in total osmotically active solutes. This experiment has added several extra dimensions to previous targeted analyses of water stress responses in Eucalyptus, and highlights that even species that are closely related (e.g. congeners) may respond differently to water stress and re-waterin
Synchrotron Radiation Dominates the Extremely Bright GRB 221009A
The brightest Gamma-ray burst, GRB 221009A, has spurred numerous theoretical
investigations, with particular attention paid to the origins of ultra-high
energy TeV photons during the prompt phase. However, analyzing the mechanism of
radiation of photons in the MeV range has been difficult because the high
flux causes pile-up and saturation effects in most GRB detectors. In this
letter, we present systematic modeling of the time-resolved spectra of the GRB
using unsaturated data obtained from Fermi/GBM (precursor) and
SATech-01/GECAM-C (main emission and flare). Our approach incorporates the
synchrotron radiation model, which assumes an expanding emission region with
relativistic speed and a global magnetic field that decays with radius, and
successfully fits such a model to the observational data. Our results indicate
that the spectra of the burst are fully in accordance with a synchrotron origin
from relativistic electrons accelerated at a large emission radius. The lack of
thermal emission in the prompt emission spectra supports a
Poynting-flux-dominated jet composition.Comment: 12 pages, 6 figures, 2 tables. Accepted for publication in ApJ
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