160 research outputs found
Are plant growth and photosynthesis limited by pre-drought following rewatering in grass?
Although the relationship between grassland productivity and soil water status has been extensively researched, the responses of plant growth and photosynthetic physiological processes to long-term drought and rewatering are not fully understood. Here, the perennial grass (Leymus chinensis), predominantly distributed in the Euro-Asia steppe, was used as an experimental plant for an irrigation manipulation experiment involving five soil moisture levels [75–80, 60–75, 50–60, 35–50, and 25–35% of soil relative water content (SRWC), i.e. the ratio between present soil moisture and field capacity] to examine the effects of soil drought and rewatering on plant biomass, relative growth rate (RGR), and photosynthetic potential. The recovery of plant biomass following rewatering was lower for the plants that had experienced previous drought compared with the controls; the extent of recovery was proportional to the intensity of soil drought. However, the plant RGR, leaf photosynthesis, and light use potential were markedly stimulated by the previous drought, depending on drought intensity, whereas stomatal conductance (gs) achieved only partial recovery. The results indicated that gs may be responsible for regulating actual photosynthetic efficiency. It is assumed that the new plant growth and photosynthetic potential enhanced by pre-drought following rewatering may try to overcompensate the great loss of the plant's net primary production due to the pre-drought effect. The present results highlight the episodic effects of drought on grass growth and photosynthesis. This study will assist in understanding how degraded ecosystems can potentially cope with climate change
A hydrothermal route to water-stable luminescent carbon dots as nanosensors for pH and temperature
Carbon dots (CDs) as a class of heavy-metal-free fluorescent nanomaterials has drawn increasing attention in recent years due to their high optical absorptivity, chemical stability, biocompatibility, and low toxicity. Herein, we report a facile method to prepare stable CDs by hydrothermal treatment of glucose (glc) in the presence of glutathione (GSH). With this approach, the formation and the surface passivation of CDs are carried out simultaneously, resulting in intrinsic fluorescence emission. The influence of reaction temperature, reaction time and feed ratio of GSH/glc on the photoluminescence property of CDs is studied. The as-prepared CDs are characterized by UV–Vis, photoluminescence, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and transmission electron microscope, from which their structural information and property are interpreted. These CDs may be useful as pH sensors or as versatile nanothermometry devices based on the pronounced temperature dependence of their steady-state fluorescence emission spectra, which changes considerably over the physiological temperature range (15–60 °C).This work was supported by the National Natural Science Foundation of China (No. 50925207), the Natural Science Foundation of Jiangsu Province, China (BK20140157), Programme of Introducing Talents of Discipline to Universities (111 Project B13025), and the Fundamental Research Funds for the Central Universities (JUSRP11418)
Loss to Follow-Up from HIV Screening to ART Initiation in Rural China.
BackgroundPatients who are newly screened HIV positive by EIA are lost to follow-up due to complicated HIV testing procedures. Because this is the first step in care, it affects the entire continuum of care. This is a particular concern in rural China.Objective(s)To assess the routine HIV testing completeness and treatment initiation rates at 18 county-level general hospitals in rural Guangxi.MethodsWe reviewed original hospital HIV screening records. Investigators also engaged with hospital leaders and key personnel involved in HIV prevention activities to characterize in detail the routine care practices in place at each county.Results699 newly screened HIV-positive patients between January 1 and June 30, 2013 across the 18 hospitals were included in the study. The proportion of confirmatory testing across the 18 hospitals ranged from 14% to 87% (mean of 43%), and the proportion of newly diagnosed individuals successfully initiated antiretroviral treatment across the hospitals ranged from 3% to 67% (mean of 23%). The average interval within hospitals for individuals to receive the Western Blot (WB) and CD4 test results from HIV positive screening (i.e. achieving testing completion) ranged from 14-116 days (mean of 41.7 days) across the hospitals. The shortest interval from receiving a positive EIA screening test result to receiving WB and CD4 testing and counseling was 0 day and the longest was 260 days.ConclusionThe proportion of patients newly screened HIV positive that completed the necessary testing procedures for HIV confirmation and received ART was very low. Interventions are urgently needed to remove barriers so that HIV patients can have timely access to HIV/AIDS treatment and care in rural China
Tunable Carbon-Dot-Based Dual-Emission Fluorescent Nanohybrids for Ratiometric Optical Thermometry in Living Cells
The use of carbon-dot-based dual-emission fluorescent nanohybrids (DEFNs) as versatile nanothermometry devices for spatially resolved temperature measurements in living cells is demonstrated. The carbon dots (CDs) are prepared in the organic phase and display tunable photoluminescence (PL) across a wide visible range by adjusting the excitation wavelengths and extend of N-doping. DEFNs are formed in a straightforward fashion from CDs (emitting blue PL) and gold nanoclusters (AuNCs, emitting red PL). The DEFNs display ideal single-excitation, dual-emission with two well-resolved, intensity-comparable fluorescence peaks, and function in optical thermometry with high reliability and accuracy by exploiting the temperature sensitivity of their fluorescence intensity ratio (blue/red). Furthermore, the DEFNs have been introduced into cells, exhibiting good biocompatibility, and have facilitated physiological temperature measurements in the range of 25-45 °C; the DEFNs can therefore function as "non-contact" tools for the accurate measurement of temperature and its gradient inside a living cell.This work was supported by the National Natural Science
Foundation of China (Nos. 50925207, 51432006, and
51503085), the Ministry of Science and Technology of China
for the International Science Linkages Program (No.
2011DFG52970), the Natural Science Foundation of Jiangsu
Province, China (No. BK20140157), the Changjiang Innovation Research Team (IRT14R23), the Programme of Introducing Talents of Discipline to Universities (111 Project
B13025). M.G.H. and C.Z. thank the Australian Research
Council (ARC) for support
Development of an incoherent broad-band cavity-enhanced aerosol extinction spectrometer and its application to measurement of aerosol optical hygroscopicity
We report on the development of a blue light-emitting-diode-based incoherent broad-band cavity-enhanced absorption spectroscopy (IBBCEAS) instrument for the measurement of the aerosol extinction coefficient at \u1d706=461 nm. With an effective absorption path length of 2.8 km, an optimum detection limit of 0.05 Mm−1 (5×10−10 cm−1) was achieved with an averaging time of 84 s. The baseline drift of the developed spectrometer was about ±0.3 Mm−1 over 2.5 h (1\u1d70e standard deviation). The performance of the system was evaluated with laboratory-generated monodispersed polystyrene latex (PSL) spheres. The retrieved complex refractive index of PSL agreed well with previously reported values. The relative humidity (RH) dependence of the aerosol extinction coefficient was measured using IBBCEAS. The measured extinction enhancement factor values for 200 nm dry ammonium sulphate particles at different RH were in good agreement with the modeled values. Field performance of the aerosol extinction spectrometer was demonstrated at the Hefei Radiation Observatory site
Environmental influences on the stable carbon isotopic composition of Devonian and Early Carboniferous land plants
Systematic analysis of the stable carbon isotopic composition of fossil land plants (δ13Cp) has the potential to offer new insights regarding paleoclimate variation and plant-environment interactions in early terrestrial ecosystems. δ13Cp was measured for 190 fossil plant specimens belonging to 10 genera of Early to Late Devonian age (Archaeopteris, Drepanophycus, Haskinsia, Leclercqia, Pertica, Psilophyton, Rhacophyton, Sawdonia, Tetraxylopteris, and Wattieza) and 2 genera of Early Carboniferous age (Genselia and Rhodeopteridium) collected from sites located mainly in the Appalachian Basin (22–30°S paleolatitude). For the full carbon-isotopic dataset (n=309), δ13Cp ranges from −20.3‰ to −30.5‰ with a mean of −25.5‰, similar to values for modern C3 land plants. In addition to a secular trend, δ13Cp exhibits both intra- and intergeneric variation. Intrageneric variation is expressed as a small (mean 0.45‰) 13C-enrichment of leaves and spines relative to stems that may reflect differential compound-specific compositions. Intergeneric variation is expressed as a much larger (to ~5‰) spread in the mean δ13Cp values of coeval plant genera that was probably controlled by taxon-specific habitat preferences and local environmental humidity. Among Early Devonian taxa, Sawdonia yielded the most 13C-depleted values (−27.1 ± 1.7‰), reflecting lower water-use efficiency that was probably related to growth in wetter habitats, and Leclercqia, Haskinsia, and Psilophyton yielded the most 13C-enriched values (−23.0 ± 1.6‰, −22.3 ± 1.3‰, and −24.8 ± 1.6‰, respectively), reflecting higher water-use efficiency probably related to growth in drier habitats
Environmental influences on the stable carbon isotopic composition of Devonian and Early Carboniferous land plants
Systematic analysis of the stable carbon isotopic composition of fossil land plants (δ13Cp) has the potential to offer new insights regarding paleoclimate variation and plant-environment interactions in early terrestrial ecosystems. δ13Cp was measured for 190 fossil plant specimens belonging to 10 genera of Early to Late Devonian age (Archaeopteris, Drepanophycus, Haskinsia, Leclercqia, Pertica, Psilophyton, Rhacophyton, Sawdonia, Tetraxylopteris, and Wattieza) and 2 genera of Early Carboniferous age (Genselia and Rhodeopteridium) collected from sites located mainly in the Appalachian Basin (22–30°S paleolatitude). For the full carbon-isotopic dataset (n = 309), δ13Cp ranges from −20.3‰ to −30.5‰ with a mean of −25.5‰, similar to values for modern C3 land plants. In addition to a secular trend, δ13Cp exhibits both intra- and intergeneric variation. Intrageneric variation is expressed as a small (mean 0.45‰) 13C-enrichment of leaves and spines relative to stems that may reflect differential compound-specific compositions. Intergeneric variation is expressed as a much larger (to ~5‰) spread in the mean δ13Cp values of coeval plant genera that was probably controlled by taxon-specific habitat preferences and local environmental humidity. Among Early Devonian taxa, Sawdonia yielded the most 13C-depleted values (−27.1 ± 1.7‰), reflecting lower water-use efficiency that was probably related to growth in wetter habitats, and Leclercqia, Haskinsia, and Psilophyton yielded the most 13C-enriched values (−23.0 ± 1.6‰, −22.3 ± 1.3‰, and −24.8 ± 1.6‰, respectively), reflecting higher water-use efficiency probably related to growth in drier habitats
A nomogram based on CT intratumoral and peritumoral radiomics features preoperatively predicts poorly differentiated invasive pulmonary adenocarcinoma manifesting as subsolid or solid lesions: a double-center study
BackgroundThe novel International Association for the Study of Lung Cancer (IASLC) grading system suggests that poorly differentiated invasive pulmonary adenocarcinoma (IPA) has a worse prognosis. Therefore, prediction of poorly differentiated IPA before treatment can provide an essential reference for therapeutic modality and personalized follow-up strategy. This study intended to train a nomogram based on CT intratumoral and peritumoral radiomics features combined with clinical semantic features, which predicted poorly differentiated IPA and was tested in independent data cohorts regarding models’ generalization ability.MethodsWe retrospectively recruited 480 patients with IPA appearing as subsolid or solid lesions, confirmed by surgical pathology from two medical centers and collected their CT images and clinical information. Patients from the first center (n =363) were randomly assigned to the development cohort (n = 254) and internal testing cohort (n = 109) in a 7:3 ratio; patients (n = 117) from the second center served as the external testing cohort. Feature selection was performed by univariate analysis, multivariate analysis, Spearman correlation analysis, minimum redundancy maximum relevance, and least absolute shrinkage and selection operator. The area under the receiver operating characteristic curve (AUC) was calculated to evaluate the model performance.ResultsThe AUCs of the combined model based on intratumoral and peritumoral radiomics signatures in internal testing cohort and external testing cohort were 0.906 and 0.886, respectively. The AUCs of the nomogram that integrated clinical semantic features and combined radiomics signatures in internal testing cohort and external testing cohort were 0.921 and 0.887, respectively. The Delong test showed that the AUCs of the nomogram were significantly higher than that of the clinical semantic model in both the internal testing cohort(0.921 vs 0.789, p< 0.05) and external testing cohort(0.887 vs 0.829, p< 0.05).ConclusionThe nomogram based on CT intratumoral and peritumoral radiomics signatures with clinical semantic features has the potential to predict poorly differentiated IPA manifesting as subsolid or solid lesions preoperatively
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