Transcriptomic profiling comparison of YAP over-expression and conditional knockout mouse tooth germs

AbstractTo identify the downstream target genes of YAP, we used RNA-Seq technology to compare the transcriptomic profilings of Yap conditional knockout (Yap CKO) and YAP over-expression mouse tooth germs. Our results showed that some Hox, Wnt and Laminin family genes had concurrent changes with YAP transcripts, indicating that the expression of these genes may be regulated by YAP. Here, we provide the detailed experimental procedure for the transcriptomic profiling results (NCBI GEO accession number GSE65524). The associated study on the regulation of Hoxa1 and Hoxc13 genes by YAP was published in Molecular Cellular Biology in 2015 [Liu et al., 2015]

Molecular docking studies on rocaglamide, a traditional Chinese medicine for periodontitis

Purpose: To undertake an in silico assessment of rocaglamide as a potential drug therapy forperiodontitis (dental arthritis).Method: Lamarckian algorithm-based automated docking approach using AutoDock4.2 tool wasapplied for calculating the best possible binding mode of rocaglamide to IL-23p19 and IL-17, the targets of anti-inflammatory drugs in periodontal disease.Results: The top two interactions of rocaglamide with IL-17 (ΔG = -5.45 and -4.83 kcal/mol) were more spontaneous, and the physical interactions (two hydrogen bonds and one π-πbond) generated in the two IL-17- rocaglamide complexes were higher in number than in IL-23p14-rocaglamide complexes.Conclusion: In silico analysis of rocaglamide, a known antimicrobial and anti-inflammatory agent, is a promising natural candidate for periodontitis therapy, and should be further subjected to in vitro and in vivo anti-periodontitis investigations.Keywords: Periodontitis, Inflammation, Rocaglamide, Molecular docking, Lamarckian algorithm, IL- 23p19, IL-1

改良穴位图谱在中医护理操作中运用效果评价

Objective To find an effective study method to master the nursing procedures of acupoints that will be applied in the clinical nursing work. Methods Based on the Chinese medicine hospital of traditional Chinese medicine nursing guidelines (trial version) published by the bureau of traditional Chinese medicine and given full expression to the advantages of the Traditional Chinese Medicine, acupoints map was improved and was applied in clinical work in August 2011 in our hospital. The nursing operation workload and learning effect in the continuity two years were compared by retrospective investigation combined with questionnaire survey. Results 1, The operation workload increases obviously and the overall growth rate in five work targets is 88.66%, after improving acupoints map of TCM nursing. 2, There are significant differences (P < 0.001) on learning interest, learning degree, memory speed, knowledge of diseas, method of operation and clinical application in our comparison.Conclusion The improved acupoints map can inspire the learning interest of the nursing stuff, help them to master common nursing acupoints of traditional Chinese medicine quickly and apply it to clinical disease. There are good social and economic benefits of this method, so it is worth promoting.目的 寻找一种有效掌握中医护理穴位的学习方法并运用于临床护理工作中。 方法 以中医药局印发《中医医院中医护理工作指南(试行)》文件的内容为指导思想，全面体现中医特色和优势。本院积极开展中医护理操作，于2011年8月设计改良穴位图谱并运用于临床。采用回顾性调查并结合问卷式调查，比较实施前后一年期间中医护理操作工作量及学习效果。结果 （1）2011年8月采用改良穴位图谱后中医护理操作工作量明显增长，五项工作量的总体增长率达88.66%；（2）采用改良穴位图谱前后护士在学习兴趣、易学程度、记忆速度、疾病腧穴知识、操作方法、临床应用等方面比较均有显著性差异（P＜0.001）。 结论 采取改良穴位图谱学习方法能提高护理人员对穴位的学习兴趣，快速掌握中医护理疾病的常用腧穴并应用于临床，赢得了较好的社会效益和经济效益，值得推广

Study on Smoke Control of Wuhan CBD Urban Traffic Link Tunnel

AbstractAn Urban Traffic Link Tunnel (UTLT) is a novel type of underground transportation system consisting of a main tunnel in a loop shape and several linked tunnels. It has a higher level of fire risk compared to other common road tunnels. In this study, numerical and experimental study has been conducted to investigate the smoke control of Wuhan Central Business District (CBD) UTLT. The transient distributions of the smoke spread have been analyzed, and the optimal smoke control strategy for the Wuhan CBD UTLT has been put forward

Adaptive Dynamic Surface Control for Generator Excitation Control System

For the generator excitation control system which is equipped with static var compensator (SVC) and unknown parameters, a novel adaptive dynamic surface control scheme is proposed based on neural network and tracking error transformed function with the following features: (1) the transformation of the excitation generator model to the linear systems is omitted; (2) the prespecified performance of the tracking error can be guaranteed by combining with the tracking error transformed function; (3) the computational burden is greatly reduced by estimating the norm of the weighted vector of neural network instead of the weighted vector itself; therefore, it is more suitable for the real time control; and (4) the explosion of complicity problem inherent in the backstepping control can be eliminated. It is proved that the new scheme can make the system semiglobally uniformly ultimately bounded. Simulation results show the effectiveness of this control scheme

The Study of Dynamic Monitor of Rice Drought in Jiangxi Province with Remote Sensing

AbstractMODIS sensor has a high temporal resolution, spectral resolution, and moderate spatial resolution. The data products are widely used in Large-scale and long period dynamic monitoring of drought. This paper focuses on characters of farming rice dominated in Jiangxi province, and analysis on remote sensing monitoring model and drought monitoring indicators which selection the MODIS data products and drought information related from 2000-2008. Established standard for classification of drought in Jiangxi province, and verified instances of 2003 drought in Jiangxi. This study may have a certain significance to agricultural drought monitoring based on rice planting in southern China, because the correspondencematch between the selected TD indices and cloud cover with precipitation and daily maximum temperature evolution curve