Anticancer bioactive peptide-3 inhibits human gastric cancer growth by targeting miR-338-5p

Additional file 1. Additional data

Research on Trust Propagation Models in Reputation Management Systems

Feedback based reputation systems continue to gain popularity in eCommerce and social media systems today and reputation management in large social networks needs to manage cold start and sparseness in terms of feedback. Trust propagation has been widely recognized as an effective mechanism to handle these problems. In this paper we study the characterization of trust propagation models in the context of attack resilience. We characterize trust propagation models along three dimensions: (i) uniform propagation and conditional propagation, (ii) jump strategies for breaking unwanted cliques, and (iii) decay factors for differentiating recent trust history from remote past history. We formally and experimentally show that feedback similarity is a critical measure for countering colluding attacks in reputation systems. Without feedback similarity guided control, trust propagations are vulnerable to different types of colluding attacks

An integrated geophysical approach for investigating hydro-geological characteristics of a debris landslide in the Wenchuan Earthquake area

Debris landslides are one of the most widely distributed types of landslides in the Wenchuan earthquake area. The hydro-geological structure characteristics are the fundamental basis for stability evaluation, performing protection and administration of a landslide. The rock and soil mass of a debris landslide was highly non-uniform and preferential seepage paths were normally developed in it. Therefore, in situ identification of the underground water seepage system became particularly important. Recently, investigations on the seepage paths of underground water in debris landslides were restricted to indoor model testing and site observation, which were far from meeting the actual demand for landslide prevention and mitigation. To locate the seepage paths, we conducted survey work on a debris landslide seated in the Xishan Village, Li County, Sichuan Province, China, by combing four different geophysical methods. They were multichannel analysis of surface wave (MASW), electrical resistivity tomography (ERT), ground penetrating radar (GPR) and microtremor survey method (MSM). The geophysical interpretation was verified with field engineering surveys and monitoring data. The results suggested that a dendritic pipe-network seepage system usually developed in debris landslides. Varisized infiltration pipes showed the characteristics of inhomogeneity and concentration of the seepage. This work highlighted that geophysical parameters (shear wave velocity Vs, dielectric constant ε and resistivity value ρ) could provide reliable qualitative and quantitative information about the colluvial layer, bedrock interface, potential sliding surface and underground water seepage system of a landslide. The optimum combination of geophysical methods was suitable to survey the hydro-geological characteristics of debris landslides in the Wenchuan earthquake area

Effect of HDAC-6 on PD cell induced by lactacystin

AbstractObjectiveTo explore the effects of histone deacetylase 6(HDAC-6) on the PD cell model induced by proteasome inhibitor lactacystin.MethodsHuman neuroblastoma SK-N-SH cells were cultured. The wild type pcDNA3.1-alpha-synuclein eukaryotic expression plasmid was transferred into the cells which then were divided into control group, group L, group T and group T+L. The cells of group L were added with 5 μmol/L lactacystin dissolved indimethylsulfoxide (DMSO) to induce PD cell model with abnormal protein aggregation, the cells of control group were treated with 5 μmol/L DMSO, the cells of group T were treated with 5 μmol/L selective HDAC-6 inhibitor tubacin dissolved in DMSO, and the cells of group T+L were treated with 5 μmol/L lactacystin and 10 μmol/L tubacin dissolved in DMSO. The expression levels of alpha-synuclein oligomers, HSP-27 and HSP-70 were detected by Western blot and the cell survival rate of all the groups was detected by MTT colorimetric assay, and compared 24 h after the cells were treated.ResultsThe expression levels of alpha-synuclein oligomers, HSP-27 and HSP-70 of the cells of group L were significantly higher than the control group, and the cell survival rate was significantly lower (P < 0.05); the expression level of alpha-synuclein oligomers of the cells of group T+L was significantly higher than group L, but the expression level of HSP-27 and HSP-70 were significantly lower, and so as the cell survival rate (P < 0.05); the differences of the expression level of alpha-synuclein oligomers, HSP-27 and HSP-70 and the cell survival rate of the cells of group T and the control group were not statistically significant (P > 0.05).ConclusionsThe expression level of alpha-synuclein oligomers can be improved and the cell survival rate can be reduced by the PD cell model induced by lactacystin and treated with selective HDAC-6 inhibitor tubacin, which means that alpha-synuclein oligomers of the PD cell model induced by lactacystin can be inhibited and the cell survival rate can be improved by HDAC-6, and the mechanism may be related to the increased of HSP-27 and HSP-70

Role of K Ca 3.1 Channels in Proliferation and Migration of Vascular Smooth Muscle Cells by Diabetic Rat Serum

Abstract Proliferation and migration of vascular smooth muscle cells (VSMCs) are important events in the development of diabetic atherosclerosis. Previous studies have suggested that K Ca 3.1 channels participate in atherosclerosis and coronary artery restenosis. In the present study, we attempted to clarify the roles of K Ca 3.1 channels in the proliferation and migration of VSMCs using experimental type-2 diabetes rat serum and aortic smooth muscle cells (SMC) prepared from non-diabetic rats. mRNA and protein levels and current density of K Ca 3.1 channels were greatly enhanced in cultured VSMCs treated with diabetic serum. In addition, diabetic serum promoted cell proliferation and migration in cultured VSMCs, and the effects were fully reversed in the cells treated with the K Ca 3.1 channels blocker TRAM-34. In conclusion, serum from diabetic rats increases the expression of K Ca 3.1 channels and promotes proliferation and migration of VSMCs to possibly participate in vascular remodeling in diabetes

Plug-and-Play of Grid-Forming Units in DC Microgrids Assisted with Power Buffers

Grid-forming units (GFUs) are fundamental devices in DC microgrids for DC bus voltage regulation. Droop control is widely used for GFU control due to its Plug-and-Play feature. However, it may fail to meet some desired DC microgrid control features and performances, such as zero steady-state DC bus voltage deviation, high system inertia, and fast DC bus voltage regulation. To achieve these desired control performances, some advanced control strategies have been proposed, but the introduction of time-dependent terms or the centralized controller in these control strategies leads to the loss of the key Plug-and-Play feature for GFUs. To maintain the Plug-and-Play feature of GFUs while meeting the desired microgrid control features and performances, this paper proposes a power buffer based control framework. The power buffer is a new device combing a capacitor and a bidirectional DC-DC converter as an interface between GFUs and the DC bus. It enables the development of a novel decoupled control strategy where GFUs are regulated by the droop control while advanced control strategies are applied to the power buffer. Thus, the Plug-and-Play feature of GFUs is maintained, while specific DC microgrid performances are also achieved simultaneously. Supported by this power buffer based control framework, GFUs can be plugged in/out DC microgrids seamlessly at any time without introducing DC bus voltage fluctuations. This allows GFUs, especially mobile GFUs, to achieve high DC bus voltage regulation performance. The hardware-in-loop (HIL) test and real circuit experimental results demonstrate its efficacy