921 research outputs found
Crocin from Crocus sativus possesses significant antiproliferation effects on human colorectal cancer cells
Aim: To investigate the anti-proliferative effects of Crocus sativus extract and its major constituent, crocin, on three colorectal cancer cell lines (HCT-116, SW-480, and HT-29). The cell growth inhibition effect was compared to that of non-small cell lung cancer (NSCLC) cells. In addition, Crocus sativus’ effect on non-cancer cells was evaluated. Methods: Using high performance liquid chromatography (HPLC), the purity of crocin and the content of crocin extract were determined. Anti-proliferative effects of Crocus sativus extract and crocin on test cells was evaluated by MTS assay. Results: The purity of crocin was found to be 95.9% and the content of crocin in the extract was 22.9%. Significant concentration-related inhibition effects of the extract on all three colorectal cancer cell lines were observed (P < 0.01). The proliferation was reduced most significantly in HCT-116 cells, to 45.5% at 1.0 mg/ml and to 6.8 % at 3.0 mg/ml. Crocin at 1.0 mM, significantly reduced HCT-116, SW-480, and HT-29 cell proliferation to 2.8%, 52%, and 16.8%, respectively (P < 0.01). Since 3.0 mg/ml Crocus sativus extract contained approximately 0.6 mM crocin, the observed effects suggest that crocin is a major responsible constituent in the extract. Significant anti-proliferative effects were also observed in non-small cell lung cancer cells. However, Crocus sativus extract did not significantly affect the growth of non-cancer young adult mouse colon cells. Conclusion: Data from this study demonstrated that Crocus sativus extract and its major constituent, crocin, significantly inhibited the growth of colorectal cancer cells while not affecting normal cells. Crocus sativus extract should be investigated further as a viable option in the treatment of colorectal cancer.Цель: исследовать антипролиферативный эффект экстракта Crocus sativus и его главного действующего вещества, кроцина,
на три клеточные линии колоректального рака человека (HCT-116, SW-480 и HT-29). Эффект ингибирования роста
клеток оценивали по отношению к таковому для клеток немелкоклеточного рака легкого (НМРЛ) и немалигнизированных
клеток. Методы: для очистки кроцина и составляющих экстракта C. sativus применяли высокоэффективную жидкостную
хроматографию (ВЭЖХ). Антипролиферативный эффект экстракта и кроцина по отношению к клеткам оценивали при
помощи MTS-теста. Результаты: степень очистки кроцина составляла 95,9%, а содержание кроцина в экстракте –22,9%.
Отмечали существенное дозозависимое угнетение пролиферации клеток трех линий экстрактом C. sativus (P < 0.01). Наиболее
выраженное снижение пролиферативной активности оотмечали для клеток HCT-116 (дo 45,5 % при 1,0 мг/мл и до 6,8 % при
3,0 мг/мл). Кроцин в концентрации 1,0 мM, значительно ингибирует пролиферацию клеток HCT-116, SW-480 и HT-29 (до 2,8,
52 и 16,8% соответственно, P < 0,01). Поскольку 3,0 мг/мл экстракта C. sativus содержат приблизительно 0,6 мM кроцина,
отмеченное действие первого подтверждает тот факт, что кроцин — главное действующее начало экстракта. Также показано
существенное антипролиферативное действие по отношению к клеткам НМРЛ. Однако C. sativus не оказывал выраженного
действия на рост немалигнизированных клеток толстого кишечника молодых мышей. Выводы: данные представленного
исследования показывают, что экстракт Crocus sativus и его главный действующий компонент кроцин значительно ингибируют
рост клеточных линий колоректального рака и в то же время не влияют на рост нормальных клеток. В дальнейшем следует
изучить возможность применения Crocus sativus для лечения больных колоректальным раком
Morphological measurements in computed tomography correlate with airflow obstruction in chronic obstructive pulmonary disease:systematic review and meta-analysis
To determine the correlation between CT measurements of emphysema or peripheral airways and airflow obstruction in chronic obstructive pulmonary disease (COPD).PubMed, Embase and Web of Knowledge were searched from 1976 to 2011. Two reviewers independently screened 1,763 citations to identify articles that correlated CT measurements to airflow obstruction parameters of the pulmonary function test in COPD patients, rated study quality and extracted information. Three CT measurements were accessed: lung attenuation area percentage <-950 Hounsfield units, mean lung density and airway wall area percentage. Two airflow obstruction parameters were accessed: forced expiratory volume in the first second as percentage from predicted (FEV1 %pred) and FEV1 divided by the forced volume vital capacity.Seventy-nine articles (9,559 participants) were included in the systematic review, demonstrating different methodologies, measurements and CT airflow obstruction correlations. There were 15 high-quality articles (2,095 participants) in the meta-analysis. The absolute pooled correlation coefficients ranged from 0.48 (95 % CI, 0.40 to 0.54) to 0.65 (0.58 to 0.71) for inspiratory CT and 0.64 (0.53 to 0.72) to 0.73 (0.63 to 0.80) for expiratory CT.CT measurements of emphysema or peripheral airways are significantly related to airflow obstruction in COPD patients. CT provides a morphological method to investigate airway obstruction in COPD.aEuro cent Computed tomography is widely performed in patients with chronic obstructive pulmonary disease (COPD)aEuro cent CT provides quantitative morphological methods to investigate airflow obstruction in COPDaEuro cent CT measurements correlate significantly with the degree of airflow obstruction in COPDaEuro cent Expiratory CT measurements correlate more strongly with airflow obstruction than inspiratory CTaEuro cent Low-dose CT decreases the radiation dose for diagnosis and quantitative emphysema evaluation.</p
An Asymmetric Cone Model for Halo Coronal Mass Ejections
Due to projection effects, coronagraphic observations cannot uniquely
determine parameters relevant to the geoeffectiveness of CMEs, such as the true
propagation speed, width, or source location. The Cone Model for Coronal Mass
Ejections (CMEs) has been studied in this respect and it could be used to
obtain these parameters. There are evidences that some CMEs initiate from a
flux-rope topology. It seems that these CMEs should be elongated along the
flux-rope axis and the cross section of the cone base should be rather
elliptical than circular. In the present paper we applied an asymmetric cone
model to get the real space parameters of frontsided halo CMEs (HCMEs) recorded
by SOHO/LASCO coronagraphs in 2002. The cone model parameters are generated
through a fitting procedure to the projected speeds measured at different
position angles on the plane of the sky. We consider models with the apex of
the cone located at the center and surface of the Sun. The results are compared
to the standard symmetric cone model
Hidden degree of freedom and critical states in a two-dimensional electron gas in the presence of a random magnetic field
We establish the existence of a hidden degree of freedom and the critical
states of a spinless electron system in a spatially-correlated random magnetic
field with vanishing mean. Whereas the critical states are carried by the
zero-field contours of the field landscape, the hidden degree of freedom is
recognized as being associated with the formation of vortices in these special
contours. It is argued that, as opposed to the coherent backscattering
mechanism of weak localization, a new type of scattering processes in the
contours controls the underlying physics of localization in the random magnetic
field system. In addition, we investigate the role of vortices in governing the
metal-insulator transition and propose a renormalization-group diagram for the
system under study.Comment: 17 pages, 16 figures; Figs. 1, 7, 9, and 10 have been reduced in
quality for e-submissio
Ganoderma lucidum extract inhibits proliferation of SW 480 human colorectal cancer cells
Aim: Ganoderma lucidum is a commonly used Chinese herb and an important ingredient in traditional Chinese medicine herbal formulations for immune dysfunction related illnesses. The effects of this medicinal mushroom on human colorectal cancer cells have not yet been evaluated. In this study, we investigated the effects of Ganoderma lucidum extract using SW 480 human colorectal cancer cell line. Materials and Methods: Two different fractions of Ganoderma lucidum extract, i.e., a fraction containing mainly polysaccharides (GLE-1), and a triterpenoid fraction without polysaccharides (GLE-2) were analyzed. Their antiproliferative activity was evaluated by cell proliferation assay and 3H-thymidine incorporation assay. Scavenging effects of DPPH radical were assessed using ESR-spectroscopy. Results: Our data showed that both GLE-1 and GLE-2 significantly inhibited the proliferation of SW 480 cells. The inhibitory effect of GLE-2 was much stronger than that of GLE-1. GLE-1 inhibited DNA synthesis in the cells and reduced the formation of DPPH radicals. Conclusion: Ganoderma lucidum extract inhibits proliferation of human colorectal cancer cells and possesses antioxidant properties.Цель: для лечения заболеваний, связанных с нарушениями функционирования иммунной системы, в традиционной китайской
медицине часто используют препараты на основе Ganoderma lucidum. В данной работе было изучено влияние экстракта Ganoderma
lucidum на клетки колоректального рака человека линии SW 480. Материалы и методы: изучали две фракции экстракта
Ganoderma lucidum: фракцию GLE-1, содержащую полисахариды, и тритерпеноидную фракцию GLE-2. Их рост-ингибирующую
активность определяли с помощью анализа клеточной пролиферации и по включению 3
H-тимидина. Для оценки антиоксидантной
активности использовали ЕСР-спектроскопию. Результаты: обе фракции Ganoderma lucidum подавляли пролиферацию клеток
SW 480. Рост-ингибирующий эффект GLE-2 был более выраженным, чем фракции GLE-1. Фракция GLE-1 подавляла синтез ДНК
в клетках линии SW 480 и понижала уровень формирования DPPH радикалов. Выводы: экстракт Ganoderma lucidum подавляет
пролиферацию клеток колоректального рака человека и обладает антиоксидантными свойствами
How Many CMEs Have Flux Ropes? Deciphering the Signatures of Shocks, Flux Ropes, and Prominences in Coronagraph Observations of CMEs
We intend to provide a comprehensive answer to the question on whether all
Coronal Mass Ejections (CMEs) have flux rope structure. To achieve this, we
present a synthesis of the LASCO CME observations over the last sixteen years,
assisted by 3D MHD simulations of the breakout model, EUV and coronagraphic
observations from STEREO and SDO, and statistics from a revised LASCO CME
database. We argue that the bright loop often seen as the CME leading edge is
the result of pileup at the boundary of the erupting flux rope irrespective of
whether a cavity or, more generally, a 3-part CME can be identified. Based on
our previous work on white light shock detection and supported by the MHD
simulations, we identify a new type of morphology, the `two-front' morphology.
It consists of a faint front followed by diffuse emission and the bright
loop-like CME leading edge. We show that the faint front is caused by density
compression at a wave (or possibly shock) front driven by the CME. We also
present high-detailed multi-wavelength EUV observations that clarify the
relative positioning of the prominence at the bottom of a coronal cavity with
clear flux rope structure. Finally, we visually check the full LASCO CME
database for flux rope structures. In the process, we classify the events into
two clear flux rope classes (`3-part', `Loop'), jets and outflows (no clear
structure). We find that at least 40% of the observed CMEs have clear flux rope
structures. We propose a new definition for flux rope CMEs (FR-CMEs) as a
coherent magnetic, twist-carrying coronal structure with angular width of at
least 40 deg and able to reach beyond 10 Rsun which erupts on a time scale of a
few minutes to several hours. We conclude that flux ropes are a common
occurrence in CMEs and pose a challenge for future studies to identify CMEs
that are clearly not FR-CMEs.Comment: 26 pages, 9 figs, to be published in Solar Physics Topical Issue
"Flux Rope Structure of CMEs
Event Reconstruction in the PHENIX Central Arm Spectrometers
The central arm spectrometers for the PHENIX experiment at the Relativistic
Heavy Ion Collider have been designed for the optimization of particle
identification in relativistic heavy ion collisions. The spectrometers present
a challenging environment for event reconstruction due to a very high track
multiplicity in a complicated, focusing, magnetic field. In order to meet this
challenge, nine distinct detector types are integrated for charged particle
tracking, momentum reconstruction, and particle identification. The techniques
which have been developed for the task of event reconstruction are described.Comment: Accepted for publication in Nucl. Instrum. A. 34 pages, 23 figure
Recommended from our members
Geomechanical/ Geochemical Modeling Studies onducted Within the International DECOVALEX Project
The DECOVALEX project is an international cooperative project initiated by SKI, the Swedish Nuclear Power Inspectorate, with participation of about 10 international organizations. The general goal of this project is to encourage multidisciplinary interactive and cooperative research on modeling coupled thermo-hydro-mechanical-chemical (THMC) processes in geologic formations in support of the performance assessment for underground storage of radioactive waste. One of the research tasks, initiated in 2004 by the U.S. Department of Energy (DOE), addresses the long-term impact of geomechanical and geochemical processes on the flow conditions near waste emplacement tunnels. Within this task, four international research teams conduct predictive analysis of the coupled processes in two generic repositories, using multiple approaches and different computer codes. Below, we give an overview of the research task and report its current status
Magnetic Flux of EUV Arcade and Dimming Regions as a Relevant Parameter for Early Diagnostics of Solar Eruptions - Sources of Non-Recurrent Geomagnetic Storms and Forbush Decreases
This study aims at the early diagnostics of geoeffectiveness of coronal mass
ejections (CMEs) from quantitative parameters of the accompanying EUV dimming
and arcade events. We study events of the 23th solar cycle, in which major
non-recurrent geomagnetic storms (GMS) with Dst <-100 nT are sufficiently
reliably identified with their solar sources in the central part of the disk.
Using the SOHO/EIT 195 A images and MDI magnetograms, we select significant
dimming and arcade areas and calculate summarized unsigned magnetic fluxes in
these regions at the photospheric level. The high relevance of this eruption
parameter is displayed by its pronounced correlation with the Forbush decrease
(FD) magnitude, which, unlike GMSs, does not depend on the sign of the Bz
component but is determined by global characteristics of ICMEs. Correlations
with the same magnetic flux in the solar source region are found for the GMS
intensity (at the first step, without taking into account factors determining
the Bz component near the Earth), as well as for the temporal intervals between
the solar eruptions and the GMS onset and peak times. The larger the magnetic
flux, the stronger the FD and GMS intensities are and the shorter the ICME
transit time is. The revealed correlations indicate that the main quantitative
characteristics of major non-recurrent space weather disturbances are largely
determined by measurable parameters of solar eruptions, in particular, by the
magnetic flux in dimming areas and arcades, and can be tentatively estimated in
advance with a lead time from 1 to 4 days. For GMS intensity, the revealed
dependencies allow one to estimate a possible value, which can be expected if
the Bz component is negative.Comment: 27 pages, 5 figures. Accepted for publication in Solar Physic
Partial Wave Analysis of
BES data on are presented. The
contribution peaks strongly near threshold. It is fitted with a
broad resonance with mass MeV, width MeV. A broad resonance peaking at 2020 MeV is also required
with width MeV. There is further evidence for a component
peaking at 2.55 GeV. The non- contribution is close to phase
space; it peaks at 2.6 GeV and is very different from .Comment: 15 pages, 6 figures, 1 table, Submitted to PL
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