A STUDY ON THE EXTRACTION AND PURIFICATION PROCESS OF LILY POLYSACCHARIDE AND ITS ANTI-TUMOR EFFECT

The objective of this paper was to extract and purify lily polysaccharide and to study its anti-H22 hepatoma effect in mice. Orthogonal experimental method was used to analyze the factors influencing the extraction and purification of lily polysaccharide, and the anti-tumor effect of lily polysaccharide was studied by acting it on H22-bearing mice. The results showed that the size of influence of various factors on the extraction results of lily polysaccharide were extraction time, extraction times and extraction temperature in decreasing order. Lily polysaccharide can enhance the immune function of H22 tumor-bearing mice, and inhibit the growth of H22 tumor. The study concluded that the optimal conditions for the extraction and purification of lily polysaccharide should be extraction times of 3 times, an extraction time of 4 h each, and an extraction temperature of 60℃; lily polysaccharide has an anti-tumor effect

Triple bottom-line consideration of sustainable plant disease management: From economic, sociological and ecological perspectives

Plant disease management plays an important role in achieving the sustainable development goals of the United Nations (UN) such as food security, human health, socio-economic improvement, resource conservation and ecological resilience. However, technologies available are often limited due to different interests between producers and society and lacks of proper understanding of economic thresholds and the complex interactions among ecology, productivity and profitability. A comprehensive synergy and conflict evaluation of economic, sociological and ecological effects with technologies, productions and evolutionary principles as main components should be used to guide sustainable disease management that aims to mitigate crop and economic losses in the short term while maintaining functional farm ecosystem in the long term. Consequently, there should be an increased emphasis on technology development, public education and information exchange among governments, researchers, producers and consumers to broaden the options for disease management in the future

Research on the Current Situation and Strategies for Enhancing the Development and Capacity of Food Safety Inspection and Testing Institutions ——A Case Study of Zhejiang Province

Food is vital to the people, and safety comes first in food. Food safety is crucial to people's livelihood, economic development, and social stability. It represents people's aspiration for a better life and grabs great public attention. In recent years, while China has achieved significant progress in food safety, the situation remains severe, with incidents such as excessive residues of agricultural and veterinary drugs, as well as illegal addition of toxic and harmful substances. As an important technical support for ensuring food safety, food safety inspection and testing institutions also exhibit a mixed bag of performance. Problems such as inadequate personnel management, improper use of facilities and equipment, weak legal awareness resulting in issuance of false reports have frequently occurred, seriously disrupting the normal order of the inspection and testing market and affecting the authority and accuracy of food safety testing. Based on the capacity verification of inspection and testing organizations in Zhejiang Province in recent years, this paper studies the typical problems faced by food inspection and testing institutions in terms of management level and testing capabilities, analyzes the root causes in depth, and proposes specific strategies from three aspects: Strengthening the main responsibility of inspection and testing institutions, combining regulatory departments with supervision and guidance, and enhancing industry self-discipline, aiming to continuously improve their inspection and testing capabilities, promote the high-quality development of food inspection and testing institutions, and effectively ensure the safety of every bite of food

A STUDY ON THE EFFECT OF RESVERATROL ON LIPID METABOLISM IN HYPERLIPIDEMIC MICE

Background: The content of resveratrol is relatively high in Polygonum cuspidatum Sieb. et Zucc., and the resveratrol has the effect of blood vessel dilating, microcirculation improving, platelet aggregation inhibiting and anti-cancer. The objective of this paper was to study the effect of resveratrol on lipid metabolism in hyperlipidemia mice. Materials and Methods: Through the establishment of an experimental mouse model of hyperlipidemia, the effect of resveratrol on change in total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-c), and low-density lipoprotein cholesterol (LDL-c) levels in mouse serum were determined. Results: Resveratrol group can apparently reduce TC, TG, LDL-c and AI of hyperlipidemic mice in a dose effect manner. Conclusion: We concluded that resveratrol can effectively reduce blood lipid levels of hyperlipidemic mice

Screening of reference genes in real-time PCR for Radopholus similis

Six candidate reference genes were chosen from the transcriptome database of Radopholus similis using the bioinformatics method, including four conventional reference genes (actin, Eukaryotic translation initiation factor 5A (eIF5A), Tubulin alpha (a-tubulin), ubiquitin (UBI)) and two new candidate reference genes (Ribosomal protein S21 (Rps21) and Serine/threonine protein phosphatase PP1-β catalytic subunit (β-PP1)). In addition, a traditional reference gene 18S ribosomal RNA (18S rRNA) obtained from NCBI databases was also added to the analysis. Real-time PCR was used to detect the expression of seven candidate reference genes in six populations of R. similis and four developmental stages (female, male, larva and egg) of a population. The stability of the expression of candidate genes was evaluated by three software programs, BestKeeper, geNorm and NormFinder. The results showed that eIF5A is the most suitable reference gene for gene functional research of different populations, while both Rps21 and eIF5A are the most suitable reference genes for different developmental stages of a population. Therefore, eIF5A is the best reference gene for studying R. similis. However, one defect of this study is that only seven candidate reference genes were analyzed; ideally, more genes should be tested

Colorimetric assay for the rapid determination of free-base nicotine in e-liquid

Nicotine exists in e-liquids primarily as the monoprotonated form and free-base form, the former is absorbed by the smoker relatively slowly and the latter is considered the bioavailable form of nicotine. Nowadays e-liquid manufacturers tend to increase nicotine in smoke aerosols, up to a content comparable to conventional cigarettes. Organic acids are added to suppress nicotine in free-base from, because the quick absorption of free-base nicotine (FBN) by the upper respiratory tract produces more bitterness and harshness to smokers. Although several methods have been developed to access FBN in conventional cigarettes or electronic cigarettes, spectrometric methods have rarely been reported. A water-solubility indicator Alizarin Red S (ARS) was introduced for the measurement of free-base nicotine. Since ARS exhibits lower acidity than organic acids, it does not compete for the tertiary amine with organic acids, but can only interact with FBN. The ARS turns from pale yellow to pink once it has been deprotonated by nicotine, and the binding constant between ARS and nicotine was determined to be 1.08 × 106 M-1. A linear calibration curve A = 0.0056c + 0.3309 with r2 = 0.9984 as a function of FBN was constructed, and applied for the evaluation of FBN in prepared e-liquid samples, with RMSE 1.12 mg g-1 for the 20 mg g-1 liquids, and 1.37 mg g-1 for the 50 mg g-1 liquids. The evaluation of FBN in commercial e-liquids agreed well with published e-liquid values. It is believed that the convenient method herein developed will be useful for manufacturers to balance the strength and harshness levels of nicotine in e-liquids.</p

The cold-induced basic helix-loop-helix transcription factor gene MdCIbHLH1 encodes an ICE-like protein in apple

<p>Abstract</p> <p>Background</p> <p>Plant growth is greatly affected by low temperatures, and the expression of a number of genes is induced by cold stress. Although many genes in the cold signaling pathway have been identified in <it>Arabidopsis</it>, little is known about the transcription factors involved in the cold stress response in apple.</p> <p>Results</p> <p>Here, we show that the apple bHLH (basic helix-loop-helix) gene <it>MdCIbHLH1 </it>(<it>Cold-Induced bHLH1</it>), which encodes an ICE-like protein, was noticeably induced in response to cold stress. The MdCIbHLH1 protein specifically bound to the MYC recognition sequences in the <it>AtCBF3 </it>promoter, and <it>MdCIbHLH1 </it>overexpression enhanced cold tolerance in transgenic <it>Arabidopsis</it>. In addition, the MdCIbHLH1 protein bound to the promoters of <it>MdCBF2 </it>and favorably contributed to cold tolerance in transgenic apple plants by upregulating the expression of <it>MdCBF2 </it>through the CBF (C-repeat-binding factor) pathway. Our findings indicate that MdCIbHLH1 functions in stress tolerance in different species. For example, ectopic <it>MdCIbHLH1 </it>expression conferred enhanced chilling tolerance in transgenic tobacco. Finally, we observed that cold induces the degradation of the MdCIbHLH1 protein in apple and that this degradation was potentially mediated by ubiquitination and sumoylation.</p> <p>Conclusions</p> <p>Based on these findings, <it>MdCIbHLH1 </it>encodes a transcription factor that is important for the cold tolerance response in apple.</p

Polymer-protein conjugate particles with biocatalytic activity for stabilization of water-in-water emulsions

Water-in-water (w/w) emulsions are attractive micro-compartmentalized platforms due to their outstanding biocom-patibility. To address the main disadvantage of poor stability that hampers their practical application, here we report a novel type of polymer-protein conjugate emulsifier obtained by Schiff base synthesis to stabilize w/w emulsions. In par-ticular, the proposed mild approach benefits the modification of proteins of suitable size and wettability as particulate emulsifiers retaining their bioactivity. As demonstrated in a model system, the methoxy polyethylene glycol (mPEG)-urease conjugate particles anchor at the w/w interfaces, where they serve as an effective emulsifier-combined-catalyst and catalyze the hydrolysis of urea in water to ammonium carbonate. Our study is unique in that it employs bioactive particles to stabilize w/w emulsions. Considering the characteristics of all-aqueous, compartmental and interfacial bio-catalysis of the system, it will open up new possibilities in the life sciences