Decay of Potential Vortex and Diffusion of Temperature in a Generalized Oldroyd-B Fluid through a Porous Medium

Based on a modified Darcy law, the decay of potential vortex and diffusion of temperature in a generalized Oldroyd-B fluid with fractional derivatives through a porous medium is studied. Exact solutions of the velocity and temperature fields are obtained in terms of the generalized Mittag-Leffler function by using the Hankel transform and discrete Laplace transform of the sequential fractional derivatives. One of the solutions is the sum of the Newtonian solutions and the non-Newtonian contributions. As limiting cases of the present solutions, the corresponding solutions of the fractional Maxwell fluid and classical Maxwell fluids are given. The influences of the fractional parameters, material parameters, and the porous space on the decay of the vortex are interpreted by graphical results

Free vibration analysis of suspended super long span CFRP cables

The dynamic characteristics of an assumed isolated CFRP main cable are studied. The section area of the CFRP cable is determined for suspension bridge in different spans and sag-span ratios based on the principle of limit design at a given security coefficient. The Irvine parameter for suspension bridge with backstay and without backstay is calculated and a rule that Irvine parameter is linearly changed with span and sag ratio respectively is found. The analysis of frequency change with span shows that the first and third symmetric frequency is lager and less than the corresponding antisymmetric frequency respectively, but the second symmetric and antisymmetric frequencies are close to each other. The analysis on frequency and dimensionless frequency change with sag ratio show that for bridge with and without backstay the first and third order symmetric frequencies are larger and less than the corresponding antisymmetric frequencies respectively, but for a bridge without backstay the crossover phenomenon happens and the second symmetric mode changes from two to four internal nodes mode

Modal analysis of cable-tower system of twin-span suspension bridge

A three-dimensional finite element model is developed in order to analyze the free vibration characteristics of the tower-cable system of a triple-tower twin-span suspension bridge during the construction phase and right after the erection of the main cable. The dynamic characteristics of each component in the tower-cable system, the isolated side span, main span cables and free-standing towers, are first analyzed separately. The natural frequencies and the vibration modes of the isolated side span and the main span cables obtained from the finite element analysis closely matched the analytical solution from the linear free vibration analysis and verified the validity of the finite element model. The local natural frequency and global natural frequency were defined to categorize the characteristics of the free vibration. The calculation results show that not only does the tower-cable system maintains the information on the modal characteristics of each component in the system, but it also contains its own unique modal characteristics and other important information about the dynamics characteristics of the system. At lower natural frequency range, the swinging motion and in-plane motion are uncoupled. The coupled modal information of the towers and the cables are separated into two groups based on the natural frequencies of the vibration of the main component of the tower-cable system. Some additional natural frequencies and vibration modes are obtained from the finite element analysis depicting the dynamic interaction between the towers and the cables. Furthermore, it is observed that the lower order modes of side span cables couple with the higher order modes of the main span cables. Either in phase or out of phase, local or global modes, the tower-cable system exhibits many new coupled mode combinations that reveal useful information

Stronger intra-specific competition aggravates negative effects of drought on the growth of Cunninghamia lanceolata

Plant-plant competition is a dynamic and complicated process that is strongly influenced by abiotic conditions. Drought is a critical threat to forests, particularly to young plantation forests. Temporal changes in competition combined with the effects of drought may dramatically influence the physiological traits of plants. Cunninghamia lanceolata plants exposed to intra-specific competition and no-competition conditions were investigated under two soil water levels (well-watered and drought). Changes in plant-plant competition relationships and nitrogen uptake rates were measured at different harvest times. The effects of drought and plant competition on physiological traits, for example, leaf nitrogen allocation, δ13C, and levels of abscisic acid (ABA), indole acetic acid (IAA) and jasmonic acid (JA), were also explored. Our results indicated that C. lanceolata shifted from intense neighbor competition to facilitation under well-watered conditions, whereas under drought neighbor competition became much stronger at the second harvest compared to the first harvest. Strong competition significantly decreased N uptake under drought. Leaf NH4+, NO3- and N allocation to water-soluble proteins increased under drought at the first harvest, but significantly declined under prolonged drought. Leaf, stem and root starch concentrations were enhanced by drought. However, during prolonged drought, the root starch concentrations, leaf δ13C, leaf ABA and starch content of C. lanceolata were much lower under strong neighbor competition than in no-competition conditions, which demonstrated that the combined effects of drought and strong competition were more harmful to plant growth and survival compared to single effects. Our study demonstrated that drought combined with competition strongly affected the N uptake, N allocation and physiological traits of plants. Intense competition imposed by neighbors is a great threat to the growth and survival of young C. lanceolata plantations under prolonged drought.Peer reviewe

Modal analysis of the triple-tower twin-span suspension bridge in deck unit erection stage

Modal analysis of large span suspension bridge in different construction stages has to be carried out for the aerodynamic instability analysis. Based on the finite element (FE) model of a triple-tower twin-span (TTTS) suspension bridge in the completed stage, the FE models of the tower-cable-deck system corresponding to 0 %, 5 %, 10 %, 20 %, 30 %, and 40 % deck units erection stage are established respectively by a backward dismantling method. Then, the dynamic characteristics of each of the tower-cable-deck systems are analyzed to study the modal properties of TTTS suspension bridge in various deck erection stages. The results demonstrate that the natural frequencies of each erection stage are closely distributed in the low-order range. In the beginning of the deck units erection stage, the tower-cable-dominated modes are the primary modes and the deck-dominated modes come to next. Due to the strong interactions between deck unites and the cables, both the in-plane and out-of-plane vibrations of cables would excite the swing, lifting or torsional modes of the deck units. Both the in-plane and out-of-plane modes including in-phase and out-of-phase modes of the two main cables in the same span or symmetry and anti-symmetry modes of the neighboring-span cables as well as their corresponding combinations can be classified into groups. With more deck unites erected, the main girder is built up gradually, and thus the frequencies of deck-dominated mode would arise. The different frequency variations result in the modal crossover phenomena, which reflect the instability of the dynamic characteristics during the long deck erection period. At last, the aerodynamic stability of the suspension bridge is checked at each early erection stage, and a wind ropes application for the aerodynamic stability enhancement is investigated. The study of dynamic characteristics provides a reference for the wind-resistance analysis of the TTTS suspension bridge during the deck units erection stage

Assessment of Night-Time Lighting for Global Terrestrial Protected and Wilderness Areas

Protected areas (PAs) play an important role in biodiversity conservation and ecosystem integrity. However, human development has threatened and affected the function and effectiveness of PAs. The Defense Meteorological Satellite Program/Operational Linescan System (DMSP/OLS) night-time stable light (NTL) data have proven to be an effective indicator of the intensity and change of human-induced urban development over a long time span and at a larger spatial scale. We used the NTL data from 1992 to 2013 to characterize the human-induced urban development and studied the spatial and temporal variation of the NTL of global terrestrial PAs. We selected seven types of PAs defined by the International Union for Conversation of Nature (IUCN), including strict nature reserve (Ia), wilderness area (Ib), national park (II), natural monument or feature (III), habitat/species management area (IV), protected landscape/seascape (V), and protected area with sustainable use of natural resources (VI). We evaluated the NTL digital number (DN) in PAs and their surrounding buffer zones, i.e., 0–1 km, 1–5 km, 5–10 km, 10–25 km, 25–50 km, and 50–100 km. The results revealed the level, growth rate, trend, and distribution pattern of NTL in PAs. Within PAs, areas of types V and Ib had the highest and lowest NTL levels, respectively. In the surrounding 1–100 km buffer zones, type V PAs also had the highest NTL level, but type VI PAs had the lowest NTL level. The NTL level in the areas surrounding PAs was higher than that within PAs. Types Ia and III PAs showed the highest and lowest NTL growth rate from 1992 to 2013, respectively, both inside and outside of PAs. The NTL distributions surrounding the Ib and VI PAs were different from other types. The areas close to Ib and VI boundaries, i.e., in the 0–25 km buffer zones, showed lower NTL levels, for which the highest NTL level was observed within the 25–100 km buffer zone. However, other types of PAs showed the opposite NTL patterns. The NTL level was lower in the distant buffer zones, and the lowest night light was within the 1–25 km buffer zones. Globally, 6.9% of PAs are being affected by NTL. Conditions of wilderness areas, e.g., high latitude regions, Tibetan Plateau, Amazon, and Caribbean, are the least affected by NTL. The PAs in Europe, Asia, and North America are more affected by NTL than South America, Africa, and Oceania

Cortical Neural Stem Cell Lineage Progression Is Regulated by Extrinsic Signaling Molecule Sonic Hedgehog.

Neural stem cells (NSCs) in the prenatal neocortex progressively generate different subtypes of glutamatergic projection neurons. Following that, NSCs have a major switch in their progenitor properties and produce γ-aminobutyric acid (GABAergic) interneurons for the olfactory bulb (OB), cortical oligodendrocytes, and astrocytes. Herein, we provide evidence for the molecular mechanism that underlies this switch in the state of neocortical NSCs. We show that, at around E16.5, mouse neocortical NSCs start to generate GSX2-expressing (GSX2+) intermediate progenitor cells (IPCs). In vivo lineage-tracing study revealed that GSX2+ IPC population gives rise not only to OB interneurons but also to cortical oligodendrocytes and astrocytes, suggesting that they are a tri-potential population. We demonstrated that Sonic hedgehog signaling is both necessary and sufficient for the generation of GSX2+ IPCs by reducing GLI3R protein levels. Using single-cell RNA sequencing, we identify the transcriptional profile of GSX2+ IPCs and the process of the lineage switch of cortical NSCs

Characterization of Zur-dependent genes and direct Zur targets in Yersinia pestis

<p>Abstract</p> <p>Background</p> <p>The zinc uptake regulator Zur is a Zn²⁺-sensing metalloregulatory protein involved in the maintenance of bacterial zinc homeostasis. Up to now, regulation of zinc homeostasis by Zur is poorly understood in <it>Y. pestis</it>.</p> <p>Results</p> <p>We constructed a <it>zur </it>null mutant of <it>Y. pestis </it>biovar <it>microtus </it>strain 201. Microarray expression analysis disclosed a set of 154 Zur-dependent genes of <it>Y. pestis </it>upon exposure to zinc rich condition. Real-time reverse transcription (RT)-PCR was subsequently used to validate the microarray data. Based on the 154 Zur-dependent genes, predicted regulatory Zur motifs were used to screen for potential direct Zur targets including three putative operons <it>znuA, znuCB </it>and <it>ykgM</it>-<it>RpmJ2</it>. The LacZ reporter fusion analysis verified that Zur greatly repressed the promoter activity of the above three operons. The subsequent electrophoretic mobility shift assay (EMSA) demonstrated that a purified Zur protein was able to bind to the promoter regions of the above three operons. The DNase I footprinting was used to identify the Zur binding sites for the above three operons, verifying the Zur box sequence as predicted previously in γ-Proteobacteria. The primer extension assay was further used to determine the transcription start sites for the above three operons and to localize the -10 and -35 elements. Zur binding sites overlapped the -10 sequence of its target promoters, which was consistent with the previous observation that Zur binding would block the entry of the RNA polymerase to repress the transcription of its target genes.</p> <p>Conclusion</p> <p>Zur as a repressor directly controls the transcription of <it>znuA, znuCB </it>and <it>ykgM</it>-<it>RpmJ2 </it>in <it>Y. pestis </it>by employing a conserved mechanism of Zur-promoter DNA association as observed in γ-Proteobacteria. Zur contributes to zinc homeostasis in <it>Y. pestis </it>likely through transcriptional repression of the high-affinity zinc uptake system ZnuACB and two alternative ribosomal proteins YkgM and RpmJ2.</p