Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

The mammalian brain exhibits marked symmetry across the sagittal plane. However, detailed description of neural dynamics in symmetric brain regions in adult mammalian animals remains elusive. In this study, we describe an experimental procedure for measuring calcium dynamics through dual optical windows above bilateral primary somatosensory corticies (S1) in Thy1-GCaMP6s transgenic mice using 2-photon (2P) microscopy. This method enables recordings and quantifications of neural activity in bilateral mouse brain regions one at a time in the same experiment for a prolonged period in vivo. Key aspects of this method, which can be completed within an hour, include minimally invasive surgery procedures for creating dual optical windows, and the use of 2P imaging. Although we only demonstrate the technique in the S1 area, the method can be applied to other regions of the living brain facilitating the elucidation of structural and functional complexities of brain neural networks

Thermodynamic and kinetic study of CO2 adsorption/desorptionon amine-functionalized sorbents

The thermodynamic and kinetic characteristics of CO2 adsorption of SBA-16 loaded with pentaethylenehexamine (PEHA) have been investigated using adsorption column system. The Langmuir isotherm model fitts the CO2 adsorption isotherms well, and the average isosteric heat of adsorption is 59.6 kJ/mol, indicating that the CO2 adsorption on PEHA-loaded SBA-16 is chemisorption. The Avrami fractional dynamics model is very suitable for illustrating the adsorption behaviour of CO2 adsorption, and the results of kinetic analysis show that increasing the partial pressure of CO2 or the working temperature is beneficial to the adsorption of CO2. Three desorption methods were evaluatedto achieve the optimal desorption method. The results show that VTSA and steam stripping method are effective methods for industrial CO2 desorption. Steam stripping may be more suitable for plants that already have low-cost steam. The activation energy Ea of CO2 adsorption/desorption is calculated using Arrhenius equation. The activation energy Ea of CO2 adsorption/desorption was calculated using the Arrhenius equation. The results show that the absolute value of Ea (adsorption) decreases with the increase of CO2 partial pressure. In addition, the Ea value of vacuum rotary regeneration method and steam stripping method is smaller than the Ea value of temperature swing regeneration

Thermodynamic and kinetic study of CO2 adsorption/desorptionon amine-functionalized sorbents

473-482The thermodynamic and kinetic characteristics of CO2 adsorption of SBA-16 loaded with pentaethylenehexamine (PEHA) have been investigated using adsorption column system. The Langmuir isotherm model fitts the CO2 adsorption isotherms well, and the average isosteric heat of adsorption is 59.6 kJ/mol, indicating that the CO2 adsorption on PEHA-loaded SBA-16 is chemisorption. The Avrami fractional dynamics model is very suitable for illustrating the adsorption behaviour of CO2 adsorption, and the results of kinetic analysis show that increasing the partial pressure of CO2 or the working temperature is beneficial to the adsorption of CO2. Three desorption methods were evaluatedto achieve the optimal desorption method. The results show that VTSA and steam stripping method are effective methods for industrial CO2 desorption. Steam stripping may be more suitable for plants that already have low-cost steam. The activation energy Ea of CO2 adsorption/desorption is calculated using Arrhenius equation. The activation energy Ea of CO2 adsorption/desorption was calculated using the Arrhenius equation. The results show that the absolute value of Ea (adsorption) decreases with the increase of CO2 partial pressure. In addition, the Ea value of vacuum rotary regeneration method and steam stripping method is smaller than the Ea value of temperature swing regeneration

Preparation and Application of Fluorescent Carbon Dots

Fluorescent carbon dots (CDs) are a novel type of fluorescent nanomaterials, which not only possess the specific quantum confinement effects of nanomaterials due to the small size of nanomaterials, but also have good biocompatibility and high fluorescence. Meanwhile, fluorescence CDs overcome the shortcomings of high toxicity of traditional nanomaterials. Moreover, the preparation procedure of fluorescent CDs is simple and easy. Therefore, fluorescent CDs have great potential applied in photocatalysis, biochemical sensing, bioimaging, drug delivery, and other related areas. In this paper, recent hot researches on fluorescent CDs are reviewed and some problems in the progress of fluorescent CDs are also summarized. At last, a future outlook in this direction is presented

Novel Y-chromosomal microdeletions associated with non-obstructive azoospermia uncovered by high throughput sequencing of sequence-tagged sites (STSs)

Y-chromosomal microdeletion (YCM) serves as an important genetic factor in non-obstructive azoospermia (NOA). Multiplex polymerase chain reaction (PCR) is routinely used to detect YCMs by tracing sequence-tagged sites (STSs) in the Y chromosome. Here we introduce a novel methodology in which we sequence 1,787 (post-filtering) STSs distributed across the entire male-specific Y chromosome (MSY) in parallel to uncover known and novel YCMs. We validated this approach with 766 Chinese men with NOA and 683 ethnically matched healthy individuals and detected 481 and 98 STSs that were deleted in the NOA and control group, representing a substantial portion of novel YCMs which significantly influenced the functions of spermatogenic genes. The NOA patients tended to carry more and rarer deletions that were enriched in nearby intragenic regions. Haplogroup O2* was revealed to be a protective lineage for NOA, in which the enrichment of b1/b3 deletion in haplogroup C was also observed. In summary, our work provides a new high-resolution portrait of deletions in the Y chromosome.National Key Scientific Program of China [2011CB944303]; National Nature Science Foundation of China [31271244, 31471344]; Promotion Program for Shenzhen Key Laboratory [CXB201104220045A]; Shenzhen Project of Science and Technology [JCYJ20130402113131202, JCYJ20140415162543017]SCI(E)[email protected]; [email protected]; [email protected]

Author Correction: The flying spider-monkey tree fern genome provides insights into fern evolution and arborescence (Nature Plants, (2022), 8, 5, (500-512), 10.1038/s41477-022-01146-6)

Correction to: Nature Plantshttps://doi.org/10.1038/s41477-022-01146-6, published online 9 May 2022. In the version of the article initially published, Dipak Khadka, who collected the samples in Nepal, was thanked in the Acknowledgements instead of being listed as an author. His name and affiliation (GoldenGate International College, Tribhuvan University, Battisputali, Kathmandu, Nepal) have been added to the authorship in the HTML and PDF versions of the article