Jahn-Teller distortion driven ferromagnetism in a perovskite fluoride monolayer

The Jahn-Teller distortion and the resulting orbital order usually cause some fascinating correlated electronic behaviors, and generally lead to antiferromagnetism in perovskite bulks. Here we demonstrate that the Jahn-Teller distortion present in the perovskite fluoride KCrF$_3$ bulk can be retained to the two-dimensional limit, resulting in a staggered orbital order and ferromagnetism in the perovskite monolayer. Octahedral tilt and rotation distortion also appear in the ground-state structure of the perovskite monolayer, which have minor effects on the electronic and magnetic properties with respect to the Jahn-Teller distortion. In addition, in the prototype phase without structural distortion, the partial occupation of the $e_g$ orbitals leads to a ferromagnetic metallic state. This work facilitates the design of two-dimensional ferromagnets and functional properties based on Jahn-Teller distortion and orbital orderComment: 8 pages, 5 figures, 1 tabl

UADB: Unsupervised Anomaly Detection Booster

Unsupervised Anomaly Detection (UAD) is a key data mining problem owing to its wide real-world applications. Due to the complete absence of supervision signals, UAD methods rely on implicit assumptions about anomalous patterns (e.g., scattered/sparsely/densely clustered) to detect anomalies. However, real-world data are complex and vary significantly across different domains. No single assumption can describe such complexity and be valid in all scenarios. This is also confirmed by recent research that shows no UAD method is omnipotent. Based on above observations, instead of searching for a magic universal winner assumption, we seek to design a general UAD Booster (UADB) that empowers any UAD models with adaptability to different data. This is a challenging task given the heterogeneous model structures and assumptions adopted by existing UAD methods. To achieve this, we dive deep into the UAD problem and find that compared to normal data, anomalies (i) lack clear structure/pattern in feature space, thus (ii) harder to learn by model without a suitable assumption, and finally, leads to (iii) high variance between different learners. In light of these findings, we propose to (i) distill the knowledge of the source UAD model to an imitation learner (booster) that holds no data assumption, then (ii) exploit the variance between them to perform automatic correction, and thus (iii) improve the booster over the original UAD model. We use a neural network as the booster for its strong expressive power as a universal approximator and ability to perform flexible post-hoc tuning. Note that UADB is a model-agnostic framework that can enhance heterogeneous UAD models in a unified way. Extensive experiments on over 80 tabular datasets demonstrate the effectiveness of UADB

Fracture Investigation of Welded Cruciform Connections

As one of the main failure modes of steel structures, fracture in welded connections has widely been discussed based on experimental investigations and numerical simulations. However, the mechanical properties of the weld and Heat Affected Zone (HAZ), such as stress-strain relationships and fracture strains under various stress states, have rarely been considered in these analyses. Therefore, in this paper, the fracture process of welded connections is discussed to investigate the effects of the inhomogeneity of mechanical properties in the weld zone. Tensile tests are conducted on welded cruciform specimens fabricated using 8 mm or 12 mm fillet welds and finite element models are developed by considering or ignoring the material inhomogeneity in the weld zone. The simulation results are compared with the experimental and it is concluded that the assumption of homogenous properties within the weld zone using the properties of the base metal will underestimate the strength of the welded cruciform specimens and using the mechanical properties of the three material areas in the weld zone will increase the accuracy of the simulation results. Using the free parameters calibrated by the fracture strains of the three material areas, the fracture process of the welded cruciform specimens is simulated using the fracture model LMVGM, and the comparison shows that the mechanical properties of the weld and HAZ should be included in the investigation of fracture in welded connections to obtain reliable simulation results

Strengths and Fracture Strains of Weld and HAZ in Welded Connections

This paper investigates the strengths and fracture strains of weld and heat affected zone (HAZ) in welded connections for both the longitudinal and transverse directions and compares them to those of the base metal. A series of miniature coupons, including miniature flat plates, notched round bars and grooved plates, were extracted from the three zones of a butt weld and tested using a custom-built jig. The true stress-strain relationships and fracture strains of the base metal, weld and HAZ materials were obtained for both directions from the miniature coupon tests and corresponding numerical simulations. The fracture strain data were used to calibrate the Lode angle modified void growth model (LMVGM) for predicting the fracture strain of the three material zones at any given stress state. The following major conclusions were drawn: (1) The weld was generally isotropic in terms of both strength and fracture strain. The weld also had the highest values of yield and tensile strengths among the three materials in both directions, but the lowest fracture strain in both directions except for the longitudinal direction with stress triaxiality above 0.21 to 0.30, for which the base metal had the lowest fracture strain. (2) The HAZ had higher yield and tensile strengths but smaller fracture strain in the longitudinal direction than in the transverse direction. The same anisotropic characteristic applied to the base metal. Meanwhile, the HAZ had higher yield and tensile strengths than the base metal as well as similar but slightly larger fracture strains in both directions. (3) The yield and tensile strengths of the weld and HAZ can be approximated using the empirical hardness-strength correlation functions, except that the functions tend to overestimate the strengths of the weld by about 10%. (4) For the weld, HAZ and base metal, the fracture surfaces tilted towards stress states with high stress triaxiality and low Lode angle parameter, indicating that fracture can initiate more easily at these stress states. Note that the above conclusions are limited to the tested AS350 grade steel and the selected welding parameters

The single-cell landscape of cystic echinococcosis in different stages provided insights into endothelial and immune cell heterogeneity

IntroductionHydatid cysts and angiogenesis are the key characteristics of cystic echinococcosis, with immune cells and endothelial cells mediating essential roles in disease progression. Recent single-cell analysis studies demonstrated immune cell infiltration after Echinococcus granulosus infection, highlighting the diagnostic and therapeutic potential of targeting certain cell types in the lesion microenvironment. However, more detailed immune mechanisms during different periods of E. granulosus infection were not elucidated.MethodsHerein, we characterized immune and endothelial cells from the liver samples of mice in different stages by single-cell RNA sequencing.ResultsWe profiled the transcriptomes of 45,199 cells from the liver samples of mice at 1, 3, and 6 months after infection (two replicates) and uninfected wild-type mice. The cells were categorized into 26 clusters with four distinct cell types: natural killer (NK)/T cells, B cells, myeloid cells, and endothelial cells. An SPP1+ macrophage subset with immunosuppressive and pro-angiogenic functions was identified in the late infection stage. Single-cell regulatory network inference and clustering (SCENIC) analysis suggested that Cebpe, Runx3, and Rora were the key regulators of the SPP1+ macrophages. Cell communication analysis revealed that the SPP1+ macrophages interacted with endothelial cells and had pro-angiogenic functions. There was an obvious communicative relationship between SPP1+ macrophages and endothelial cells via Vegfa–Vegfr1/Vegfr2, and SPP1+ macrophages interacted with other immune cells via specific ligand–receptor pairs, which might have contributed to their immunosuppressive function.DiscussionOur comprehensive exploration of the cystic echinococcosis ecosystem and the first discovery of SPP1+ macrophages with infection period specificity provide deeper insights into angiogenesis and the immune evasion mechanisms associated with later stages of infection

Impact of initial water vapor on the upscale growing process of a squall line in South China

During the spring and summer seasons, in the South China region where abundant water vapor is present, squall lines can rapidly develop into larger scales within a short period of time. In order to explore the influence of water vapor content on the process of squall line scale growth in South China, using the WRF model, a numerical simulation was conducted for a squall line system in South China on 11 May 2020. We investigated the effect of the variation of water vapor at different levels on its intensity and structure, and discussed the growth mechanism of the squall line system. This squall line occurred with the presence of high-level jet and low-level wind shear complementing each other, within an unstable layer of "dry at the high level and wet at the low level". The simulation showed that, in the early stage of the squall line development, a large maximum convective available potential energy (MCAPE) was observed in the southern part of the convection and coastal warm areas, which is beneficial for the accumulation of unstable energy here. Meanwhile, with the high low-level wind shear, the linear structure of the convection was well maintained. Subsequently, the squall line propagated southward and merged with warm region convection, resulting a further scale growth. Water vapor experiments showed that the MCAPE values are primarily influenced by the moisture content in the low-level atmosphere. More low-level moisture content causes stronger thunderstorm high pressure. Additionally, the presence of higher MCAPE values and larger low-level vertical wind shear contribute to the growth of convective cells in the post-convective stage, prolonging their existence. Reducing the mid-level water vapor content results in a decrease in intense surface precipitation, a weakening of convective intensity, and a quick dissipation into individual convective cells. But when the squall line moves into the area with high MCAPE values, it once again develops into a linear structure. Therefore, an increase in low-level moisture or a decrease in mid-level moisture favors the genesis of convection. However, reducing mid-level moisture results in relatively drier air at mid-levels, making it difficult for the linear structure to be sustained. Further investigation into the internal structure of the squall line reveals that vertical motion and rear inflow also influence the scale growth of the squall line. In the convective system analyzed in this study, the strong rearward inflow enhances the upward motion and generates forward outflow, leading to severe surface wind. Strengthening low-level moisture not only increases the size of the stratiform cloud region at the rear of the convection, but also leads to a stronger upward motion sustaining vertically, which promotes prolonged convective activity. On the other hand, reducing mid-level moisture weakens convective intensity and lowers the height of the echo tops. During the development stage, the rearward inflow intensifies, and dry cold air descends rapidly, leading to the strengthening of the surface cold pool, and causing strong winds due to the forward outflow

High expression of ubiquitin-conjugating enzyme 2C (UBE2C) correlates with nasopharyngeal carcinoma progression

BACKGROUND: Overexpression of ubiquitin-conjugating enzyme 2C (UBE2C) has been detected in many types of human cancers, and is correlated with tumor malignancy. However, the role of UBE2C in human nasopharyngeal carcinoma (NPC) is unclear. In this study, we investigated the role of aberrant UBE2C expression in the progression of human NPC. METHODS: Immunohistochemical analysis was performed to detect UBE2C protein in clinical samples of NPC and benign nasopharyngeal tissues, and the association of UBE2C expression with patient clinicopathological characteristics was analyzed. UBEC2 expression profiles were evaluated in cell lines representing varying differentiated stages of NPC and immortalized nasopharyngeal epithelia NP-69 cells using quantitative RT-PCR, western blotting and fluorescent staining. Furthermore, UBE2C was knocked down using RNA interference in these cell lines and proliferation and cell cycle distribution was investigated. RESULTS: Immunohistochemical analysis revealed that UBE2C protein expression levels were higher in NPC tissues than in benign nasopharyngeal tissues (P<0.001). Moreover, high UBE2C protein expression was positively correlated with tumor size (P=0.017), lymph node metastasis (P=0.016) and distant metastasis (P=0.015) in NPC patients. In vitro experiments demonstrated that UBE2C expression levels were inversely correlated with the degree of differentiation of NPC cell lines, whereas UBE2C displayed low level of expression in NP-69 cells. Knockdown of UBE2C led to significant arrest at the S and G2/M phases of the cell cycle, and decreased cell proliferation was observed in poorly-differentiated CNE2Z NPC cells and undifferentiated C666-1 cells, but not in well-differentiated CNE1 and immortalized NP-69 cells. CONCLUSIONS: Our findings suggest that high expression of UBE2C in human NPC is closely related to tumor malignancy, and may be a potential marker for NPC progression

Transcriptome analysis of two pepper genotypes infected with pepper mild mottle virus

Pepper mild mottle virus (PMMoV) poses a significant threat to pepper production because it is highly contagious and extremely persistent in soil. Despite this threat, little is known about the molecular processes that underlie plant responses to pepper mild mottle virus. Here, we performed RNA sequencing of tolerant (“17-p63”) and susceptible (“16-217”) pepper genotypes after pepper mild mottle virus or mock inoculation. Viral accumulation in systemic leaves was lower in the pepper mild mottle virus-resistant 17-p63 genotype than in the pepper mild mottle virus-sensitive 16-217 genotype, and infection symptoms were more apparent in systemic leaves of 16-217 than in those of 17-p63 at the same timepoints during the infection process. We identified 2,959 and 2,159 differentially expressed genes (DEGs) in systemic leaves of infected 16-217 and 17-p63, respectively. Through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of differentially expressed genes from both genotypes revealed significant enrichment of the MAPK signaling pathway, plant–pathogen interaction, and flavonoid biosynthesis. A number of differentially expressed genes showed opposite trends in relation to stress resistance and disease defense in the two genotypes. We also performed weighted gene co-expression network analysis (WGCNA) of all samples and identified modules associated with resistance to pepper mild mottle virus, as well as seven hub genes. These results identify candidate virus resistance genes and provide insight into pepper defense mechanisms against pepper mild mottle virus

Extensive loss of cell-cycle and DNA repair genes in an ancient lineage of bipolar budding yeasts

Cell-cycle checkpoints and DNA repair processes protect organisms from potentially lethal mutational damage. Compared to other budding yeasts in the subphylum Saccharomycotina, we noticed that a lineage in the genus Hanseniaspora exhibited very high evolutionary rates, low Guanine–Cytosine (GC) content, small genome sizes, and lower gene numbers. To better understand Hanseniaspora evolution, we analyzed 25 genomes, including 11 newly sequenced, representing 18/21 known species in the genus. Our phylogenomic analyses identify two Hanseniaspora lineages, a faster-evolving lineage (FEL), which began diversifying approximately 87 million years ago (mya), and a slower-evolving lineage (SEL), which began diversifying approximately 54 mya. Remarkably, both lineages lost genes associated with the cell cycle and genome integrity, but these losses were greater in the FEL. E.g., all species lost the cell-cycle regulator WHIskey 5 (WHI5), and the FEL lost components of the spindle checkpoint pathway (e.g., Mitotic Arrest-Deficient 1 [MAD1], Mitotic Arrest-Deficient 2 [MAD2]) and DNA-damage–checkpoint pathway (e.g., Mitosis Entry Checkpoint 3 [MEC3], RADiation sensitive 9 [RAD9]). Similarly, both lineages lost genes involved in DNA repair pathways, including the DNA glycosylase gene 3-MethylAdenine DNA Glycosylase 1 (MAG1), which is part of the base-excision repair pathway, and the DNA photolyase gene PHotoreactivation Repair deficient 1 (PHR1), which is involved in pyrimidine dimer repair. Strikingly, the FEL lost 33 additional genes, including polymerases (i.e., POLymerase 4 [POL4] and POL32) and telomere-associated genes (e.g., Repressor/ activator site binding protein-Interacting Factor 1 [RIF1], Replication Factor A 3 [RFA3], Cell Division Cycle 13 [CDC13], Pbp1p Binding Protein [PBP2]). Echoing these losses, molecular evolutionary analyses reveal that, compared to the SEL, the FEL stem lineage underwent a burst of accelerated evolution, which resulted in greater mutational loads, homopolymer instabilities, and higher fractions of mutations associated with the common endogenously damaged base, 8-oxoguanine. We conclude that Hanseniaspora is an ancient lineage that has diversified and thrived, despite lacking many otherwise highly conserved cell-cycle and genome integrity genes and pathways, and may represent a novel, to our knowledge, system for studying cellular life without them.Fil: Steenwyk, Jacob L.. Vanderbilt University; Estados UnidosFil: Opulente, Dana A.. University of Wisconsin; Estados UnidosFil: Kominek, Jacek. University of Wisconsin; Estados UnidosFil: Shen, Xing-Xing. Vanderbilt University; Estados UnidosFil: Zhou, Xiaofan. South China Agricultural University; ChinaFil: Labella, Abigail L.. Vanderbilt University; Estados UnidosFil: Bradley, Noah P.. Vanderbilt University; Estados UnidosFil: Eichman, Brandt F.. Vanderbilt University; Estados UnidosFil: Cadez, Neza. University of Ljubljana; EsloveniaFil: Libkind Frati, Diego. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; ArgentinaFil: DeVirgilio, Jeremy. United States Department of Agriculture. Agricultural Research Service; ArgentinaFil: Hulfachor, Amanda Beth. University of Wisconsin; Estados UnidosFil: Kurtzman, Cletus P.. United States Department of Agriculture. Agricultural Research Service; ArgentinaFil: Hittinger, Chris Todd. University of Wisconsin; Estados UnidosFil: Rokas, Antonis. Vanderbilt University; Estados Unido