MiR-138 ameliorates myocardial ischemia/reperfusion injury by targeting intercellular cell adhesion molecule 1

Purpose: To explore the effect of miR-138 on regulating intercellular cell adhesion molecule 1 (ICAM-1) expression in endothelial cells to alleviate cardiac ischemia/reperfusion (I/R) injury and its related mechanisms. Methods: The left anterior descending artery of the heart was occluded for 30 min and then perfused for 2 h to induce a rat model of cardiac I/R injury. H9C2 cells were cultured in an anoxic medium without serum to establish the model of hypoxia/reoxygenation (H/R). Triphenyl tetrazolium chloride (TTC) staining was applied to measure myocardial infarction sizes in rat hearts. The mRNA expression levels of miR-138 and ICAM-1 were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). Dual luciferase reporter assay was used to identify the target of miR-138. The agomiR-138 and miR-138 mimics were transfected into H9C2 cells; exogenous ICAM-1 was also administered, and ROS accumulation, cell viability, and apoptosis were measured. Furthermore, the underlying mechanism was investigated. Results: MiR-138 was downregulated both in vitro and in vivo. AgomiR-138 reduced myocardial infarction area, decreased ROS production and suppressed cell apoptosis in a rat model of cardiac I/R injury. On the other hand, miR-138 mimics increased cell viability, enhanced ROS production and induced cell apoptosis in H/R-induced H9C2 cells. Further analysis verified ICAM-1 as a target of miR- 138. Besides, exogenous ICAM-1 inhibited the protective effect of miR-138 on H/R-induced apoptosis in vitro. Conclusion: MiR-138 may protect against injury of myocardial I/R by targeting ICAM-1. The results also provide insight into miR-138/ICAM-1 axis as new therapeutic targets for myocardial I/R injury. Keywords: Intercellular cell adhesion molecule 1, MicroRNA-138, Myocardial/ischemia reperfusion injury, Reactive oxygen specie

The Application of the Multiple Points of a Mold Function of UG MoldWizard in the Design of Cutting Compressor

In recent years, mold industry develops rapidly. The design and manufacture of mold have become an important symbol to measure the level of a country#39s manufacturing industry. Difficult point in the design of the cutting compressor for recycling plastic bottles lies in cutting and compressing plastic bottles at the same time. Use UG MoldWizard to design the working process of injection mold. Design and manufacture the U-lower plastic mold with the aid of a computer. Make optimal design plan of the U-lower plastic in the cutting compressor

Comparative metabolomics analysis of milk components between Italian Mediterranean buffaloes and Chinese Holstein cows based on LC-MS/MS technology

Buffalo and cow milk have a very different composition in terms of fat, protein, and total solids. For a better knowledge of such a difference, the milk metabolic profiles and characteristics of metabolites was investigated in Italian Mediterranean buffaloes and Chinese Holstein cows were investigated by liquid chromatography tandem-mass spectrometry (LC-MS/MS) in this study. Totally, 23 differential metabolites were identified to be significantly different in the milk from the two species of which 15 were up-regulated and 8 down-regulated in Italian Mediterranean buffaloes. Metabolic pathway analysis revealed that 4 metabolites (choline, acetylcholine, nicotinamide and uric acid) were significantly enriched in glycerophospholipid metabolism, nicotinate and nicotinamide metabolism, glycine, serine and threonine metabolism, as well as purine metabolism. The results provided further insights for a deep understanding of the potential metabolic mechanisms responsible for the different performance of Italian Mediterranean buffaloes’ and Chinese Holstein cows’ milk. The findings will offer new tools for the improvement and novel directions for the development of dairy industry

Quantitative analysis of water vapor budget of a persistent rainstrom event in Tongren of Guizhou Province

This paper focus on revealing the features of the water vapor transport, water vapor budget and the contribution percentage of water vapor source regions of the persistent rainstorm in Tongren from July 13th to 16th, 2014 based on precipitation observation data, ERA5 and NCEP GDAS (National Centers for Environmental Prediction, Global Data Assimilation System) reanalysis data and the HYSPLIT4 (Hybrid Single-Particle Lagrangian Integrated Trajectory Model) mode. The results show that: (1) the eastward South Asian High and coupling mechanism of high and low level jets enhanced the dynamic mechanism of convergence at low level and divergence at high level which is conducive to the convergence of water vapor in the target region to condense and then form precipitation. (2) The water vapor over the ocean was continuously transported to the rainstorm area by the water vapor channel built by the synergistic effect of the subtropical high which table controlled the south of Guizhou province, shortwave trough lay the northwest side of subtropical high, and tropical cyclone in the Indian Peninsula at 500 hPa. (3) The air particles in the rainstorm area mainly came from the Arabian Sea, the Bay of Bengal and the South China Sea at a lower height, while a few particles came from the north of Tongren to Eurasia, the Atlantic Ocean at a higher height by 120 h backward trajectory simulation. (4) The contribution percentage of water vapor source regions of the south of Tongren-South China Sea and its nearby islands and water, the east of the Indian Peninsula-the Bay of Bengal, the Arabian Sea-the west of the Indian Peninsula were 48.29%, 32.17 % and 10.47% respectively. In addition, the water vapor the north of Tongren to Eurasia and the Atlantic Ocean also contributed to the rainstorm in Tongren (9.07%). (5) 850 hPa and 700 hPa were the main water vapor contribution levels which provided nearly 3/4 of water vapor to the rainstorm area, the remaining quarter was transported by 500 hPa

Ghrelin regulates hyperactivity-like behaviors via growth hormone signaling pathway in zebrafish (Danio rerio)

IntroductionGhrelin is originally identified as the endogenous ligand for the growth hormone secretagogue receptor (GHSR) and partially acts by stimulating growth hormone (GH) release. Our previous studies have identified GHRELIN as a novel susceptibility gene for human attention-deficit hyperactivity disorder (ADHD), and ghrelin-depleted zebrafish (Danio rerio) display ADHD-like behaviors. However, the underlying molecular mechanism how ghrelin regulates hyperactivity-like behaviors is not yet known.ResultsHere, we performed RNA-sequencing analysis using adult ghrelinΔ/Δ zebrafish brains to investigate the underlying molecular mechanisms. We found that gh1 mRNA and genes related to the gh signaling pathway were significantly reduced at transcriptional expression levels. Quantitative polymerase chain reaction (qPCR) was performed and confirmed the downregulation of gh signaling pathway-related genes in ghrelinΔ/Δ zebrafish larvae and the brain of adult ghrelinΔ/Δ zebrafish. In addition, ghrelinΔ/Δ zebrafish displayed hyperactive and hyperreactive phenotypes, such as an increase in motor activity in swimming test and a hyperreactive phenotype under light/dark cycle stimulation, mimicking human ADHD symptoms. Intraperitoneal injection of recombinant human growth hormone (rhGH) partially rescued the hyperactivity and hyperreactive-like behaviors in ghrelin mutant zebrafish. ConclusionOur results indicated that ghrelin may regulate hyperactivity-like behaviors by mediating gh signaling pathway in zebrafish. And the protective effect of rhGH on ghrelinΔ/Δ zebrafish hyperactivity behavior provides new therapeutic clues for ADHD patients

Assessment of multi-source observation merged 1 km-grid precipitation product during the disastrous rainstorms in Guangdong

This paper aims to assess the latest 1 km-grid Analysis Real Time (ART_1 km) precipitation product developed by the National Meteorological Information Center of China Meteorological Administration (CMA), which can provide great support for disaster weather monitoring and warning, intelligent grid forecasting and weather services. Observed precipitation data from the independent stations (including non-uploaded regional meteorological stations and hydrometric stations) that were not integrated into the ART_1 km precipitation product as well as precipitation classification inspection are used to assess the quality of this product during twenty disastrous rainstorm cases from May to August during 2019-2022 in Guangdong. The results show that the ART_1 km precipitation product successfully reproduces the precipitation location, strength, and trends in these cases, with the best performance in the Pearl River Delta, the east of eastern Guangdong, and the north of northern Guangdong. The stronger the precipitation, the greater the correlation as well as the root mean square error (RMSE) and mean error (ME) between the ART_1 km precipitation and the observed precipitation. When the hourly precipitation is not classified, about 60% of these independent stations present a correlation efficient ≥ 0.8, more than 90% of the stations present an RMSE within the range of [1.0, 5.0) mm, and more than 60% of the stations present a ME within ±0.1 mm. When the hourly precipitation is < 5 mm, most of the stations have a correlation efficient < 0.5, an RMSE within the range of [1.0, 5.0) mm, and a ME within [0.0, 0.5] mm. When the hourly precipitation is ≥ 20 mm, 42%~56% of the stations have a correlation efficient ≥ 0.5, and most of the stations have an RMSE ≥ 10 mm and a ME < 0 mm, even when the hourly precipitation is ≥ 50 mm, most of the stations have a ME < -10 mm. Overall, ART_1 km precipitation is usually underestimated at the independent stations, and integrating observations from more sites into producing ART_1 km precipitation is helpful to improve the quality of the products

On the Origin of the Strong Optical Variability of Emission-line Galaxies

Emission-line galaxies (ELGs) are crucial in understanding the formation and evolution of galaxies, while little is known about their variability. Here we report the study on the optical variability of a sample of ELGs selected in the COSMOS field, which has narrow-band observations in two epochs separated by $\gtrsim$ 12 years. This sample was observed with Suprime-Cam (SC) and Hyper Suprime-Cam (HSC) on the $Subaru$ telescope in NB816 and $i'/i$ bands, respectively. After carefully removing the wing effect of a narrow-band filter, we check the optical variability in a sample of 181 spectroscopically confirmed ELGs. We find that 0 (0/68) Ha emitters, 11.9% (5/42) [OIII] emitters, and 0 (0/71) [OII] emitters show significant variability ($|\Delta m_{NB}| \geq 3\,\sigma_{\Delta m_{NB,AGN}} = 0.20\, mag$) in the two-epoch narrow-band observations. We investigate the presence of active galactic nucleus (AGN) in this variable ELG (var-ELG) sample with three methods, including X-ray luminosity, mid-infrared activity, and radio-excess. We find zero bright AGN in this var-ELG sample, but cannot rule out the contribution from faint AGN. We find that SNe could also dominate the variability of the var-ELG sample. The merger morphology shown in the HST/F814W images of all the var-ELG sample is in agreement with the enhancement of star formation, i.e., the SNe activity.Comment: 20 pages, 10 figures, accepted for publication in The Astrophysical Journa

Discovery of Five Green Pea Galaxies with Double-peaked Narrow [OIII] Lines

Although double-peaked narrow emission-line galaxies have been studied extensively in the past years, only a few are reported with the green pea galaxies (GPs). Here we present our discovery of five GPs with double-peaked narrow [OIII] emission lines, referred to as DPGPs, selected from the LAMOST and SDSS spectroscopic surveys. We find that these five DPGPs have blueshifted narrow components more prominent than the redshifted components, with velocity offsets of [OIII]$\lambda$5007 lines ranging from 306 to 518 $\rm km\, s^{-1}$ and full widths at half maximums (FWHMs) of individual components ranging from 263 to 441 $\rm km\, s^{-1}$. By analyzing the spectra and the spectral energy distributions (SEDs), we find that they have larger metallicities and stellar masses compared with other GPs. The H$\alpha$ line width, emission-line diagnostic, mid-infrared color, radio emission, and SED fitting provide evidence of the AGN activities in these DPGPs. They have the same spectral properties of Type 2 quasars. Furthermore, we discuss the possible nature of the double-peaked narrow emission-line profiles of these DPGPs and find that they are more likely to be dual AGN. These DPGP galaxies are ideal laboratories for exploring the growth mode of AGN in the extremely luminous emission-line galaxies, the co-evolution between AGN and host galaxies, and the evolution of high-redshift galaxies in the early Universe.Comment: 13 pages, 8 figures, 3 tables; Accepted for publication in MNRA