36 research outputs found
Surgical excision promotes tumor growth and metastasis by promoting expression of MMP-9 and VEGF in a breast cancer model
Surgery is still the main curative therapeutic modality for breast cancer. Although surgery often results in the successful removal of the primary tumor, its process could increase the risk of metastases of residual cancer cells. Understanding of the connection between breast cancer metastasis and surgical wound will lead to the establishment of a proper treatment strategy for postoperative cancer patient. Aim: To study the influence of surgical procedure on the metastasis of primary breast cancer. Methods: We established MDA-MB-435 human breast cancer xenograft model. Levels of Pro-matrix metalloproteinase 9 (Pro-MMP-9) and vascular endothelial growth factor (VEGF) in host serum and tumors were tested at different time points with ELISA and zymography and correlated to tumor growth and postoperative metastasis. Results: Our study demonstrated surgical wound had promoting effect on tumor growth and pulmonary metastasis of human breast cells, if tumor cells remain in bodies. This effect might be related to the postoperative interaction of cancer and host cells, which resulted in expression of Pro-MMP-9. Surgical process could also increase the VEGF expression in tumor tissues. Conclusions: Surgical wound-produced host Pro-MMP-9 and tumor cell VEGF might be important mediators leading to metastasis of residual breast cancer after surgery.Π₯ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π»Π΅ΡΠ΅Π½ΠΈΠ΅ Π΄ΠΎ ΡΠΈΡ
ΠΏΠΎΡ ΠΎΡΡΠ°Π΅ΡΡΡ Π³Π»Π°Π²Π½ΡΠΌ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΠΎΠΌ ΠΏΡΠΈ Π»Π΅ΡΠ΅Π½ΠΈΠΈ Π±ΠΎΠ»ΡΠ½ΡΡ
ΡΠ°ΠΊΠΎΠΌ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ
ΠΆΠ΅Π»Π΅Π·Ρ. Π₯ΠΎΡΡ Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΡΠ΄Π°Π»Π΅Π½ΠΈΠ΅ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π² Π±ΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²Π΅ ΡΠ»ΡΡΠ°Π΅Π² Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎ, Π² ΡΠΎ ΠΆΠ΅ Π²ΡΠ΅ΠΌΡ ΠΎΠ½ΠΎ ΠΌΠΎΠΆΠ΅Ρ
ΡΠ²Π΅Π»ΠΈΡΠΈΡΡ ΡΠΈΡΠΊ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΎΡΡΠ°ΡΠΎΡΠ½ΡΡ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ. ΠΠΎΠ½ΠΈΠΌΠ°Π½ΠΈΠ΅ ΡΠ²ΡΠ·ΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΏΡΠΈ ΡΠ°ΠΊΠ΅
ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΠΈ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠ°Π½ΠΎΠΉ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΡ Π²ΡΠ±ΡΠ°ΡΡ Π½Π°ΠΈΠ»ΡΡΡΡΡ ΡΡΡΠ°ΡΠ΅Π³ΠΈΡ ΠΏΠΎΡΡΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ Π»Π΅ΡΠ΅Π½ΠΈΡ. Π¦Π΅Π»Ρ:
ΠΎΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π²ΠΌΠ΅ΡΠ°ΡΠ΅Π»ΡΡΡΠ²Π° Π½Π° ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ. ΠΠ΅ΡΠΎΠ΄Ρ: Π±ΡΠ»Π° ΡΠΎΠ·Π΄Π°Π½Π° ΠΌΠΎΠ΄Π΅Π»Ρ ΡΠ°ΠΊΠ°
ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Π² Π²ΠΈΠ΄Π΅ ΠΊΡΠ΅Π½ΠΎΡΡΠ°Π½ΡΠΏΠ»Π°Π½ΡΠ°ΡΠ° MDA-MB-435. Π£ΡΠΎΠ²Π΅Π½Ρ Pro-ΠΌΠ°ΡΡΠΈΡΠ½ΠΎΠΉ ΠΌΠ΅ΡΠ°Π»Π»ΠΎΠΏΡΠΎΡΠ΅ΠΈΠ½Π°Π·Ρ 9
(Pro-MMP-9) ΠΈ ΡΠ°ΠΊΡΠΎΡΠ° ΡΠΎΡΡΠ° ΡΠ½Π΄ΠΎΡΠ΅Π»ΠΈΡ ΡΠΎΡΡΠ΄ΠΎΠ² (VEGF) Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΡΠ΅ΡΠΈΠΏΠΈΠ΅Π½ΡΠ° ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΡΡ
ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈΡΡ Π² ΡΠ°Π·Π½ΡΠ΅
Π²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠ΅ ΠΏΡΠΎΠΌΠ΅ΠΆΡΡΠΊΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ELISA ΠΈ Π·ΠΈΠΌΠΎΠ³ΡΠ°ΡΠΈΠΈ. ΠΡΠΈ ΡΡΠΎΠΌ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Π½Π°Π»ΠΈΡΠΈΠ΅ ΠΊΠΎΡΠ΅Π»Π»ΡΡΠΈΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΡΡΠΈΠΌΠΈ ΠΏΠΎΠΊΠ°Π·Π°- ΠΈ Π·ΠΈΠΌΠΎΠ³ΡΠ°ΡΠΈΠΈ. ΠΡΠΈ ΡΡΠΎΠΌ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Π½Π°Π»ΠΈΡΠΈΠ΅ ΠΊΠΎΡΠ΅Π»Π»ΡΡΠΈΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΡΡΠΈΠΌΠΈ ΠΏΠΎΠΊΠ°Π·Π° ΠΈ Π·ΠΈΠΌΠΎΠ³ΡΠ°ΡΠΈΠΈ. ΠΡΠΈ ΡΡΠΎΠΌ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Π½Π°Π»ΠΈΡΠΈΠ΅ ΠΊΠΎΡΠ΅Π»Π»ΡΡΠΈΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΡΡΠΈΠΌΠΈ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΡΠΌΠΈ,
ΡΠΎΡΡΠΎΠΌ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΈ ΠΏΠΎΡΡΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΡΠΌΠΈ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·Π°ΠΌΠΈ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: Π±ΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½Π°Ρ ΡΠ°Π½Π° ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ
ΡΠΎΡΡΡ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΠΈ Π΅Π΅ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π² Π»Π΅Π³ΠΊΠΈΠ΅ Π² ΡΠ»ΡΡΠ°Π΅, ΠΊΠΎΠ³Π΄Π° ΠΏΡΠΈ ΡΠ΄Π°Π»Π΅Π½ΠΈΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π² ΡΠ°Π½Π΅
ΠΎΡΡΠ°ΡΡΡΡ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ. ΠΡΠΎ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΡΠ²ΡΠ·Π°Π½ΠΎ Ρ ΠΏΠΎΡΡΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΡΠΌ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΈ ΠΎΠΊΡΡΠΆΠ°ΡΡΠΈΡ
ΠΈΡ
Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ, ΠΊΠΎΡΠΎΡΠΎΠ΅ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Pro-MMP-9. Π£Π΄Π°Π»Π΅Π½ΠΈΠ΅ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΌΠΎΠΆΠ΅Ρ ΡΠ°ΠΊΠΆΠ΅ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΠΎΠ²Π°ΡΡ
ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ VEGF ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ. ΠΡΠ²ΠΎΠ΄Ρ: Pro-MMP-9, ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΡΠ΅ΠΌΡΠΉ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ,
ΠΎΠΊΡΡΠΆΠ°ΡΡΠΈΠΌΠΈ ΠΏΠΎΡΡΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΡΡ ΡΠ°Π½Ρ, Π° ΡΠ°ΠΊΠΆΠ΅ VEGF, ΠΏΡΠΎΠ΄ΡΡΠΈΡΡΠ΅ΠΌΡΠΉ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ, ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ Π²Π°ΠΆΠ½ΡΠΌΠΈ
ΠΌΠ΅Π΄ΠΈΠ°ΡΠΎΡΠ°ΠΌΠΈ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΎΡΡΠ°ΡΠΎΡΠ½ΡΡ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠΎΡΠ»Π΅ Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π²ΠΌΠ΅ΡΠ°ΡΠ΅Π»ΡΡΡΠ²Π°
Stable Autonomous Robotic Wheelchair Navigation in the Environment with Slope Way
In this article, we present a path planning approach that is capable of generating a feasible trajectory for stable robotic wheelchair navigation in the environment with slope way. Firstly, the environment is modeled by a lightweight navigation map, with which the proposed sampling-based path planning scheme with a modified extension function can generate a feasible path. Then, the path is further optimized by the proposed utility function involving the human comfort and the path cost. To improve the searching efficiency of an optimal trajectory, we present an adaptive weighting Gaussian Mixture Model (GMM) based sampling strategy. Particularly, the weights of the components in GMM are adjusted adaptively in the planning process. It is also worth noting that the proposed sampling-based planning paradigm can indicate the unsafe regions in the navigation map, which forms a traversable map and further guarantees the safety of the wheelchair robot navigation. Furthermore, the effectiveness and the efficiency of the proposed path planning method are verified in both simulation and real-world experiments. Β© 1967-2012 IEEE
Comparison of efficacy and toxicity between gemcitabine plus cisplatin and plus carboplatin in first-line treatment of advanced non-small cell lung cancer
High precision interval analysis of the frequency response of structural-acoustic systems with uncertain-but-bounded parameters
Association between the Polymorphism of CCR5 and Alzheimer's Disease: results of a study performed on male and female patients from Northern Italy
CCR5 receptor. Biologic and genetic implications in age-related diseases.
The CC chemokine receptor 5 (CCR5) is a member of CCchemokine receptor family. CCR5 has the characteristic structure of a seven transmembrane G protein\u2013coupled receptor (GPCR), which regulates trafficking and effector functions of memory/effector Th1 cells, macrophages, NK cells, and immature dendritic cells. CCR5 and its ligands are important molecules in viral pathogenesis. CCR5 represents the co-receptor for macrophage (M) and dual (T cell and M)-tropic immunodeficiency viruses. Recent evidence has also demonstrated the role of CCR5 in a variety of human diseases, ranging from infectious and inflammatory diseases to cancer. In this article, we describe the involvement of CCR5 in two age-related diseases, atherosclerosis and Alzheimer\u2019s disease, suggesting a possible role of chemokine system on these diseases\u2019 pathophysiology. Finally,we reviewthe data on the probable association between CCR5\u39432 deletion and cardiovascular diseases and Alzheimer\u2019s disease