Note sur la microflore bactérienne intestinale d'un Nématode : Thelazia rhodesi

L'intestin de Thelazia rhodesi récolté au Sénégal, et observé au microscope électronique, contient une population bactérienne importante. Ces bactéries, ensemencées sur milieu gélosé, se révèlent généralement être des cultures pures, monospécifiques (genres Corynebacterium et Aeromonas en particulier). Leur pouvoir pathogène, faible in vitro, pourrait devenir important in vivo où ils vivent soustraits à l'action des substances bactériostatiques de l'oi

The DAMA/LIBRA apparatus

The $\simeq$ 250 kg highly radiopure NaI(Tl) DAMA/LIBRA apparatus, running at the Gran Sasso National Laboratory (LNGS) of the I.N.F.N., is described.Comment: 37 pages, 27 figure

Self-consistent simulation of plasma scenarios for ITER using a combination of 1.5D transport codes and free-boundary equilibrium codes

Self-consistent transport simulation of ITER scenarios is a very important tool for the exploration of the operational space and for scenario optimisation. It also provides an assessment of the compatibility of developed scenarios (which include fast transient events) with machine constraints, in particular with the poloidal field (PF) coil system, heating and current drive (H&CD), fuelling and particle and energy exhaust systems. This paper discusses results of predictive modelling of all reference ITER scenarios and variants using two suite of linked transport and equilibrium codes. The first suite consisting of the 1.5D core/2D SOL code JINTRAC [1] and the free boundary equilibrium evolution code CREATE-NL [2,3], was mainly used to simulate the inductive D-T reference Scenario-2 with fusion gain Q=10 and its variants in H, D and He (including ITER scenarios with reduced current and toroidal field). The second suite of codes was used mainly for the modelling of hybrid and steady state ITER scenarios. It combines the 1.5D core transport code CRONOS [4] and the free boundary equilibrium evolution code DINA-CH [5].Comment: 23 pages, 18 figure

Further results from DAMA/LIBRA-phase2 and perspectives

The data collected by the DAMA/LIBRA-phase2 set-up during two additional annual cycles have been analyzed, further investigating the long-standing model-independent annual modulation effect pointed out by DAMA deep underground at the Gran Sasso National Laboratory of the I.N.F.N. by using various different experimental configurations. Including the new results, the total exposure of DAMA/LIBRA-phase2 over 8 annual cycles is 1.53 t·yr and the evidence for a signal that meets all the requirements of the model-independent Dark Matter annual modulation signature is 11.8 σ C.L. in the energy region (1 - 6) keV. In the (2 - 6) keV energy interval, where data are also available from DAMA/NaI and DAMA/LIBRA-phase1, the achieved C.L. for the full exposure of 2.86 t·yr is 13.7 σ. No systematics or side reaction able to mimic this signature (i.e., to account for the whole measured modulation amplitude and to simultaneously satisfy all the requirements of the signature) has been found or suggested by anyone throughout some decades thus far. A preliminary result on the further lowering of the software energy threshold and perspectives are also mentioned

Interferon regulatory factor 8-deficiency determines massive neutrophil recruitment but T cell defect in fast growing granulomas during tuberculosis

Following Mycobacterium tuberculosis (Mtb) infection, immune cell recruitment in lungs is pivotal in establishing protective immunity through granuloma formation and neogenesis of lymphoid structures (LS). Interferon regulatory factor-8 (IRF-8) plays an important role in host defense against Mtb, although the mechanisms driving anti-mycobacterial immunity remain unclear. In this study, IRF-8 deficient mice (IRF-8−/−) were aerogenously infected with a low-dose Mtb Erdman virulent strain and the course of infection was compared with that induced in wild-type (WT-B6) counterparts. Tuberculosis (TB) progression was examined in both groups using pathological, microbiological and immunological parameters. Following Mtb exposure, the bacterial load in lungs and spleens progressed comparably in the two groups for two weeks, after which IRF-8−/− mice developed a fatal acute TB whereas in WT-B6 the disease reached a chronic stage. In lungs of IRF-8−/−, uncontrolled growth of pulmonary granulomas and impaired development of LS were observed, associated with unbalanced homeostatic chemokines, progressive loss of infiltrating T lymphocytes and massive prevalence of neutrophils at late infection stages. Our data define IRF-8 as an essential factor for the maintenance of proper immune cell recruitment in granulomas and LS required to restrain Mtb infection. Moreover, IRF-8−/− mice, relying on a common human and mouse genetic mutation linked to susceptibility/severity of mycobacterial diseases, represent a valuable model of acute TB for comparative studies with chronically-infected congenic WT-B6 for dissecting protective and pathological immune reactions

Acute lymphoblastic leukemia displays a distinct highly methylated genome

DNA methylation is tightly regulated during development and is stably maintained in healthy cells. In contrast, cancer cells are commonly characterized by a global loss of DNA methylation co-occurring with CpG island hypermethylation. In acute lymphoblastic leukemia (ALL), the commonest childhood cancer, perturbations of CpG methylation have been reported to be associated with genetic disease subtype and outcome, but data from large cohorts at a genome-wide scale are lacking. Here, we performed whole-genome bisulfite sequencing across ALL subtypes, leukemia cell lines and healthy hematopoietic cells, and show that unlike most cancers, ALL samples exhibit CpG island hypermethylation but minimal global loss of methylation. This was most pronounced in T cell ALL and accompanied by an exceptionally broad range of hypermethylation of CpG islands between patients, which is influenced by TET2 and DNMT3B. These findings demonstrate that ALL is characterized by an unusually highly methylated genome and provide further insights into the non-canonical regulation of methylation in cancer