NASA applications project in Miami County, Indiana

The study site selection is intended to serve all of the different research areas within the project, i.e., soil conditions, soil management, etc. There are seven major soil associations or soils formed on similar landscapes in the Miami Co., and over 38 soil series that were mapped. Soil sampling was conducted in some sites because of its variability in soils and cover types, variable topography, and presence of erosion problems. Results from analysis of these soil data is presented

New leontiniid Notoungulata (Mammalia) from Chile and Argentina : comparative anatomy, character analysis, and phylogenetic hypotheses. (American Museum novitates, no. 3737)

64 p. : ill. (1 col.), map ; 26 cm.Herein we describe and name two new species of leontiniid notoungulates, one being the first known from Chile, the other from the Deseadan South American Land Mammal Age (SALMA) of Patagonia, Argentina. The Chilean leontiniid is from the lower horizons of the Cura-Mallín Formation (Tcm1) at Laguna del Laja in the Andean Main Range of central Chile. This new species, Colpodon antucoensis, is distinguishable from Patagonian species of Colpodon by way of its smaller I2; larger I3 and P1; sharper, V-shaped snout; and squarer upper premolars. The holotype came from a horizon that is constrained below and above by 40Ar/39Ar ages of 19.53 ± 0.60 and 19.25 ± 1.22, respectively, suggesting an age of roughly 19.5 Ma, or a little older ( 19.8 Ma) when corrected for a revised age of the Fish Canyon Tuff standard. Either age is slightly younger than ages reported for the Colhuehuapian SALMA fauna at the Gran Barranca. Taxa from the locality of the holotype of C. antucoensis are few, but they (e.g., the mylodontid sloth, Nematherium, and a lagostomine chinchillid) also suggest a post-Colhuehuapian faunal age. The second leontiniid named in this paper has been known in the literature for over 75 years as Leontinia sp. Several specimens referable to this species were discovered at Pico Truncado (Deseadan SALMA) during the Field Museums first Marshall Field Expedition, led by Elmer Riggs in 1924. This “new” taxon, Elmerriggsia fieldia, is a small-bodied leontiniid, possessing grooved premolar protocones that lack intermediate lingual cingulae, but have well-developed labial cingulids on their lower molars. This new taxon is fairly common at Pico Truncado, in Santa Cruz, Argentina, but we have not encountered it at other localities. The character-taxon matrix that we constructed for this analysis differs from those previously developed for notoungulates by the substantially greater number of postcranial characters used (41). Colbertia magellanica was used as the outgroup in all analyses. Our initial phylogenetic analysis was limited to only taxa traditionally assigned to the Toxodontia. These included a dozen taxa traditionally considered to be leontiniids, two toxodontids, four notohippids, a homalodotheriid, and two isotemnids. The taxa traditionally classified as leontiniids formed a monophyletic group, in which V-shaped muzzle, caniniform i3, femur with medial suprapatellar ridge, and large wedge-shaped fibular facet of the calcaneum were unequivocal synapomorphies. Colpodon spp. nested within a clade that includes the “tropical” leontiniids, Taubatherium and Huilatherium. Toxodontids and notohippids formed a monophyletic group sister to the leontiniids, with these two clades forming a more inclusive clade that previously had been called the “advanced Toxodontia.” However, when five species of typotheres from three “families” were added to the analysis, the “notohippid” Eurygenium was identified as the nearest outgroup of leontiniids and an “advanced notohippid” plus toxodontid clade (nodes C + F). Unequivocal synapomorphies uniting these two nodes were robust calcanonavicular articulation (“reverse alternating tarsus” as evidenced by a distinct navicular facet on the calcaneum) and a distal radius with a styloid process. The presence of an entolophid fossettid in the lower molars and the downturned olecranon process of the ulna were equivocal synapomorphies for this clade. Though lacking the character states that diagnose a more exclusive “notohippid-toxodontid-leontiniid” clade, Eurygenium shared several unequivocal synapomorphies that unite it with these taxa. These include a well-formed fossette of upper molars formed by the posterior cingulum, absence of an entepicondylar foramen of the humerus, lack of a neck on the astragalus, a transversely elongated astragalar head, and absence of the “astragalar buttress” of the navicular. Unconventionally, the interatheriids used in the analysis (Federicoanaya and Protypotherium, both interatheriine interatheriids) formed the sister group to the taxa traditionally considered to be the “advanced Toxodontia.” Unequivocal synapomorphies uniting these interatheriids with the “advanced Toxodontia” are exclusively postcranial: tetradactyl manus, quadrate fibular facet of the calcaneum, calcaneonavicular contact (without well-formed facet on the calcaneum), and union of the groove for the tendon of the flexor hallucis longus with the astragalar trochlea. Steeply inclined ectal facets of the astragalus and calcaneum are equivocal synapomorphies (shared with Eurygenium, the notohippids, and toxodontids, but not leontiniids). Inclusion of postcranial characters in the phylogenetic analysis illustrates an otherwise undetectable conflict—that of homoplasy-homology discordance between dental and postcranial characters of interatheriine interatheriids (the postcranial skeleton of “notopithicine” interatheriids [or “basal interatheriids” of Hitz et al., 2006] remain unknown). This conflict does not simply represent an arcane point, but has relevance regarding reconstructing the interrelation-ships of several major groups of notoungulates. Other findings of this work include a northerly extension of the geographical range of Colpodon and a possible temporal extension beyond the Colhuehuapian SALMA. It appears that the fauna at Laguna del Laja is an important source of information regarding the faunal transition that occurred between Colhuehuapian and Santacrucian SALMA faunas

Chlorocyon phantasma, a late Eocene borhyaenoid (Mammalia, Metatheria, Sparassodonta) from the Los Helados locality, Andean Main Range, central Chile. (American Museum novitates, 0003-0082 ; no. 3918)

22 pages : illustrations (some color), map ; 26 cm.Sparassodont metatherians were the dominant terrestrial mammalian predators during South America's long Cenozoic isolation. This group's early fossil record is very poor, however, particularly for the late Eocene and early Oligocene. Here, we describe a new sparassodont, Chlorocyon phantasma, gen. et sp. nov., based on a specimen from Los Helados, a new locality within the Abanico Formation of the Andean Main Range of central Chile. New ⁴⁰Ar/³⁹Ar dates at Los Helados bracketing the fossil-bearing level constrain the age of this specimen to 37-36 Ma (late Eocene), indicating that this new taxon likely pertains to the Mustersan South American Land Mammal "Age." Chlorocyon is the first Paleogene sparassodont reported from Chile and the first sparassodont described from the Abanico Formation. Distinctive features, including a p3 with an anterior edge that is more curved than the posterior edge and the lack of a hypoconulid on m4, suggest that Chlorocyon is a borhyaenoid closely related to Pharsophorus or Plesiofelis, although much smaller. Chlorocyon represents a welcome addition to the sparse record of late Eocene sparassodonts and indicates that the diversity of non-proborhyaenid borhyaenoids prior to the late Oligocene was greater than previously thought

Chlorocyon phantasma, a late Eocene borhyaenoid (Mammalia, Metatheria, Sparassodonta) from the Los Helados locality, Andean Main Range, central Chile /

no. 391

New leontiniid Notoungulata (Mammalia) from Chile and Argentina : comparative anatomy, character analysis, and phylogenetic hypotheses /

no. 373

Effects of the Absence of Apolipoprotein E on Lipoproteins, Neurocognitive Function, and Retinal Function

IMPORTANCE: The identification of a patient with a rare form of severe dysbetalipoproteinemia allowed the study of the consequences of total absence of apolipoprotein E (apoE). OBJECTIVES: To discover the molecular basis of this rare disorder and to determine the effects of complete absence of apoE on neurocognitive and visual function and on lipoprotein metabolism. DESIGN, SETTING, AND PARTICIPANTS: Whole-exome sequencing was performed on the patient’s DNA. He underwent detailed neurological and visual function testing and lipoprotein analysis. Lipoprotein analysis was also performed in the Cardiovascular Research Institute, University of California, San Francisco, on blood samples from the proband’s mother, wife, 2 daughters, and normolipidemic control participants. MAIN OUTCOME MEASURES: Whole-exome sequencing, lipoprotein analysis, and neurocognitive function. RESULTS: The patient was homozygous for an ablative APOE frameshift mutation (c.291del, p.E97fs). No other mutations likely to contribute to the phenotype were discovered, with the possible exception of two, in ABCC2 (p.I670T) and LIPC (p.G137R). Despite complete absence of apoE, he had normal vision, exhibited normal cognitive, neurological, and retinal function, had normal findings on brain magnetic resonance imaging, and had normal cerebrospinal fluid levels of β-amyloid and tau proteins. He had no significant symptoms of cardiovascular disease except a suggestion of myocardial ischemia on treadmill testing and mild atherosclerosis noted on carotid ultrasonography. He had exceptionally high cholesterol content (760 mg/dL; to convert to millimoles per liter, multiply by 0.0259) and a high cholesterol to triglycerides ratio (1.52) in very low-density lipoproteins with elevated levels of small-diameter high-density lipoproteins, including high levels of prebeta-1 high-density lipoprotein. Intermediate-density lipoproteins, low-density lipoproteins, and very low-density lipoproteins contained elevated apoA-I and apoA-IV levels. The patient’s apoC-III and apoC-IV levels were decreased in very low-density lipoproteins. Electron microscopy revealed large lamellar particles having electron-opaque cores attached to electron-lucent zones in intermediate-density and low-density lipoproteins. Low-density lipoprotein particle diameters were distributed bimodally. CONCLUSIONS AND RELEVANCE: Despite a profound effect on lipoprotein metabolism, detailed neurocognitive and retinal studies failed to demonstrate any defects. This suggests that functions of apoE in the brain and eye are not essential or that redundant mechanisms exist whereby its role can be fulfilled. Targeted knockdown of apoE in the central nervous system might be a therapeutic modality in neurodegenerative disorders

Effects of the Absence of Apolipoprotein E on Lipoproteins, Neurocognitive Function, and Retinal Function

ImportanceThe identification of a patient with a rare form of severe dysbetalipoproteinemia allowed the study of the consequences of total absence of apolipoprotein E (apoE).ObjectivesTo discover the molecular basis of this rare disorder and to determine the effects of complete absence of apoE on neurocognitive and visual function and on lipoprotein metabolism.Design, setting, and participantsWhole-exome sequencing was performed on the patient's DNA. He underwent detailed neurological and visual function testing and lipoprotein analysis. Lipoprotein analysis was also performed in the Cardiovascular Research Institute, University of California, San Francisco, on blood samples from the proband's mother, wife, 2 daughters, and normolipidemic control participants.Main outcome measuresWhole-exome sequencing, lipoprotein analysis, and neurocognitive function.ResultsThe patient was homozygous for an ablative APOE frameshift mutation (c.291del, p.E97fs). No other mutations likely to contribute to the phenotype were discovered, with the possible exception of two, in ABCC2 (p.I670T) and LIPC (p.G137R). Despite complete absence of apoE, he had normal vision, exhibited normal cognitive, neurological, and retinal function, had normal findings on brain magnetic resonance imaging, and had normal cerebrospinal fluid levels of β-amyloid and tau proteins. He had no significant symptoms of cardiovascular disease except a suggestion of myocardial ischemia on treadmill testing and mild atherosclerosis noted on carotid ultrasonography. He had exceptionally high cholesterol content (760 mg/dL; to convert to millimoles per liter, multiply by 0.0259) and a high cholesterol to triglycerides ratio (1.52) in very low-density lipoproteins with elevated levels of small-diameter high-density lipoproteins, including high levels of prebeta-1 high-density lipoprotein. Intermediate-density lipoproteins, low-density lipoproteins, and very low-density lipoproteins contained elevated apoA-I and apoA-IV levels. The patient's apoC-III and apoC-IV levels were decreased in very low-density lipoproteins. Electron microscopy revealed large lamellar particles having electron-opaque cores attached to electron-lucent zones in intermediate-density and low-density lipoproteins. Low-density lipoprotein particle diameters were distributed bimodally.Conclusions and relevanceDespite a profound effect on lipoprotein metabolism, detailed neurocognitive and retinal studies failed to demonstrate any defects. This suggests that functions of apoE in the brain and eye are not essential or that redundant mechanisms exist whereby its role can be fulfilled. Targeted knockdown of apoE in the central nervous system might be a therapeutic modality in neurodegenerative disorders