Teilnahmemotive von marathon- und ultramarathonläufern

Etude sur la motivation des coureurs de longue distance. Différences des motifs de participation selon la spécialité, marathon ou ultramarathon, et l'âge des coureurs : objectif d'amélioration de la santé, recherche d'une estime sociale élevée ..

Epidemic Achromobacter xylosoxidans strain among Belgian cystic fibrosis patients and review of literature

Background: Achromobacter xylosoxidans is increasingly being recognized as an emerging pathogen in cystic fibrosis. Recent severe infections with A. xylosoxidans in some of our cystic fibrosis (CF) patients led to a re-evaluation of the epidemiology of CF-associated A. xylosoxidans infections in two Belgian reference centres (Antwerp and Ghent). Several of these patients also stayed at the Rehabilitation Centre De Haan (RHC). In total, 59 A. xylosoxidans isolates from 31 patients (including 26 CF patients), collected between 2001 and 2014, were studied. We evaluated Matrix Assisted Laser Desorption Ionisation -Time of Flight mass spectrometry (MALDI-TOF) as an alternative for McRAPD typing. Results: Both typing approaches established the presence of a major cluster, comprising isolates, all from 21 CF patients, including from two patients sampled when staying at the RHC a decade ago. This major cluster was the same as the cluster established already a decade ago at the RHC. A minor cluster consisted of 13 isolates from miscellaneous origin. A further seven isolates, including one from a non-CF patient who had stayed recently at the RHC, were singletons. Conclusions: Typing results of both methods were similar, indicating transmission of a single clone of A. xylosoxidans among several CF patients from at least two reference centres. Isolates of the same clone were already observed at the RHC, a decade ago. It is difficult to establish to what extent the RHC is the source of transmission, because the epidemic strain was already present when the first epidemiological study in the RHC was carried out. This study also documents the applicability of MALDI-TOF for typing of strains within the species A. xylosoxidans and the need to use the dynamic cutoff algorithm of the BioNumerics (R) software for correct clustering of the fingerprints

Additional file 1: Figure S1. of Epidemic Achromobacter xylosoxidans strain among Belgian cystic fibrosis patients and review of literature

Detailed presentation of MALDI-TOF main spectra (MSPs) of 59 isolates (with isolate CF GUH 86–1 tested in duplo). A: dendrogram based on UPGMA; B: detail of the MaldiTOF MS spectra (2000–4000 m/z); C: cluster 1 (red) and cluster 2 (green); D: similarity matrix based on the Pearson correlation. (PDF 117 kb

Methylome-wide analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences

International audienceA growing number of children born are conceived through in vitro fertilisation (IVF), which has been linked to an increased risk of adverse perinatal outcomes, as well as altered growth profiles and cardiometabolic differences in the resultant individuals. Some of these outcomes have also been shown to be influenced by the use of different IVF culture media and this effect is hypothesised to be mediated epigenetically, e.g. through the methylome. As such, we profiled the umbilical cord blood methylome of IVF neonates that underwent preimplantation embryo development in two different IVF culture media (G5 or HTF), using the Infinium Human Methylation EPIC BeadChip. We found no significant methylation differences between the two groups in terms of: (i) systematic differences at CpG sites or regions, (ii) imprinted sites/genes or birth weight-associated sites, (iii) stochastic differences presenting as DNA methylation outliers or differentially variable sites, and (iv) epigenetic gestational age acceleration