24 research outputs found
Binding of pRNA to the N-terminal 14 amino acids of connector protein of bacteriophage phi29
During assembly, bacterial virus phi29 utilizes a motor to insert genomic DNA into a preformed protein shell called the procapsid. The motor contains one twelve-subunit connector with a 3.6 nm central channel for DNA transportation, six viral-encoded RNA (packaging RNA or pRNA) and a protein, gp16, with unknown stoichiometry. Recent DNA-packaging models proposed that the 5-fold procapsid vertexes and 12-fold connector (or the hexameric pRNA ring) represented a symmetry mismatch enabling production of a force to drive a rotation motor to translocate and compress DNA. There was a discrepancy regarding the location of the foothold for the pRNA. One model [C. Chen and P. Guo (1997) J. Virol., 71, 3864â3871] suggested that the foothold for pRNA was the connector and that the pRNAâconnector complex was part of the rotor. However, one other model suggested that the foothold for pRNA was the 5-fold vertex of the capsid protein and that pRNA was the stator. To elucidate the mechanism of phi29 DNA packaging, it is critical to confirm whether pRNA binds to the 5-fold vertex of the capsid protein or to the 12-fold symmetrical connector. Here, we used both purified connector and purified procapsid for binding studies with in vitro transcribed pRNA. Specific binding of pRNA to the connector in the procapsid was found by photoaffinity crosslinking. Removal of the N-terminal 14 amino acids of the gp10 protein by proteolytic cleavage resulted in undetectable binding of pRNA to either the connector or the procapsid, as investigated by agarose gel electrophoresis, SDSâPAGE, sucrose gradient sedimentation and N-terminal peptide sequencing. It is therefore concluded that pRNA bound to the 12-fold symmetrical connector to form a pRNAâconnector complex and that the foothold for pRNA is the connector but not the capsid protein
Determination of the rate limiting step during zearalenone hydrolysis by ZenA
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The low intestinal and hepatic toxicity of hydrolyzed fumonisin B1 correlates with its inability to alter the metabolism of sphingolipids
Fumonisins are mycotoxins frequently found as natural contaminants in maize, where they are produced by the plant pathogen Fusarium verticillioides. They are toxic to animals and exert their effects through mechanisms involving disruption of sphingolipid metabolism.Fumonisin B1 (FB1) is the predominant fumonisins in this family. FB1 is converted to its hydrolyzed analogs HFB1, by alkaline cooking (nixtamalization) or through enzymatic degradation. The toxicity of HFB1 is poorly documented especially at the intestinal level. The objectives of this study were to compare the toxicity of HFB1 and FB1 and to assess the ability of these toxins to disrupt sphingolipids biosynthesis. HFB1 was obtained by a deesterification of FB1, with a carboxylesterase. Piglets, animals highly sensitive to FB1, were exposed by gavage for 2 weeks to 2.8 ”mol FB1 or HFB1/kg body weight/day. FB1 induced hepatotoxicity as indicated by the lesion score, the level of several biochemical analytes and the expression of inflammatory cytokines. Similarly, FB1 impaired the morphology of the different section of the small intestine, reduced villi height and modified intestinal cytokine expression. By contrast, HFB1 did not trigger hepatotoxicity, did not impair intestinal morphology and slightly modified the intestinal immune response. This low toxicity of HFB1 correlates with a weak alteration of the sphinganine/sphingosine ratio in the liver and in the plasma. Taken together, these data demonstrate that HFB1 does not cause intestinal or hepatic toxicity in the sensitive pig model, and slightly disrupts sphingolipids metabolism. This finding suggests that conversion to HFB1 could be a good strategy to reduce FB1 exposure
Detoxification of the fumonisin mycotoxins in maize : an enzymatic approach
CITATION: Alberts, J., et al. 2019. Detoxification of the fumonisin mycotoxins in maize : an enzymatic approach. Toxins, 11(9):523, doi:10.3390/toxins11090523.The original publication is available at https://www.mdpi.comEnzymatic detoxification has become a promising approach for control of mycotoxins postharvest in grains through modification of chemical structures determining their toxicity. In the present study fumonisin esterase FumD (EC 3.1.1.87) (FUMzymeÂź; BIOMIN, Tulln, Austria), hydrolysing fumonisin (FB) mycotoxins by de-esterification, was utilised to develop an enzymatic reduction method in a maize kernel enzyme incubation mixture. Efficacy of the FumD FB reduction method in âlowâ and âhighâ FB contaminated home-grown maize was compared by monitoring FB1 hydrolysis to the hydrolysed FB1 (HFB1) product utilising a validated LC-MS/MS analytical method. The method was further evaluated in terms of enzyme activity and treatment duration by assessing enzyme kinetic parameters and the relative distribution of HFB1 between maize kernels and the residual aqueous environment. FumD treatments resulted in significant reduction (â„80%) in âlowâ (â„1000 U/L, p < 0.05) and âhighâ (100 U/L, p < 0.05; â„1000 U/L, p < 0.0001) FB contaminated maize after 1 h respectively, with an approximate 1:1 ”mol conversion ratio of FB1 into the formation of HFB1. Enzyme kinetic parameters indicated that, depending on the activity of FumD utilised, a significantly (p < 0.05) higher FB1 conversion rate was noticed in âhighâ FB contaminated maize. The FumD FB reduction method in maize could find application in commercial maize-based practices as well as in communities utilising home-grown maize as a main dietary staple and known to be exposed above the tolerable daily intake levels.https://www.mdpi.com/2072-6651/11/9/523Publisher's versio
Effects of orally administered fumonisin B1 (FB1), partially hydrolysed FB1, hydrolysed FB1 and N-(1-deoxy-D-fructos-1-yl) FB1 on the sphingolipid metabolism in rats
Fumonisin B1 (FB1) is a Fusarium mycotoxin frequently occurring in maize-based food and feed. Alkaline
processing like nixtamalisation of maize generates partially and fully hydrolysed FB1 (pHFB1 and HFB1)
and thermal treatment in the presence of reducing sugars leads to formation of N-(1-deoxy-D-fructos-
1-yl) fumonisin B1 (NDF). The toxicity of these metabolites, in particular their effect on the sphingolipid
metabolism, is either unknown or discussed controversially.We produced high purity FB1, pHFB1a+b, HFB1
and NDF and fed them to male Sprague Dawley rats for three weeks. Once a week, urine and faeces samples
were collected over 24 h and analysed for fumonisin metabolites as well as for the sphinganine (Sa) to
sphingosine (So) ratio by validated LCâMS/MS based methods. While the latter was significantly increased
in the FB1 positive control group, the Sa/So ratios of the partially and fully hydrolysed fumonisins
were indifferent from the negative control group. Although NDF was partly cleaved during digestion, the
liberated amounts of FB1 did not raise the Sa/So ratio. These results show that the investigated alkaline
and thermal processing products of FB1 were, at the tested concentrations, non-toxic for rats, and suggest
that according food processing can reduce fumonisin toxicity for humans
Controlling bacteriophage phi29 DNA-packaging motor by addition or discharge of a peptide at N-terminus of connector protein that interacts with pRNA
Bacteriophage phi29 utilizes a motor to translocate genomic DNA into a preformed procapsid. The motor contains six pRNAs, an enzyme and one 12-subunit connector with a central channel for DNA transportation. A 20-residue peptide containing a His-tag was fused to the N-terminus of the connector protein gp10. This fusion neither interfered with procapsid assembly nor affected the morphology of the prolate-shaped procapsid. However, the pRNA binding and virion assembly activity were greatly reduced. Such decreased functions can be switched back on by the removal of the tag via protease cleavage, supporting the previous finding that the N-terminus of gp10 is essential for the pRNA binding. The DNA-packaging efficiency with dimeric pRNA was more seriously affected by the extension than with monomeric pRNA. It is speculated that the fusion of the tag generated physical hindrance to pRNA binding, with greater influence for the dimers than the monomers due to their size. These results reveal a potential to turn off and turn on the motor by attaching or removing, respectively, a component to outer part of the motor, and offers an approach for the inhibition of viral replication by using a drug or a small peptide targeted to motor components
A total of 5â20% sucrose gradient sedimentation of procapsidâpRNA complex treated before and after V8 treatment
<p><b>Copyright information:</b></p><p>Taken from "Binding of pRNA to the N-terminal 14 amino acids of connector protein of bacteriophage phi29"</p><p>Nucleic Acids Research 2005;33(8):2640-2649.</p><p>Published online 10 May 2005</p><p>PMCID:PMC1092275.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> The closed rhombus is the procapsidâpRNA complex, while the open triangle is V8 pre-treated procapsid before adding pRNA (). The open square is V8-treated procapsidâpRNA complex ()
Specific binding of PpRNA I-iâČ to connector demonstrated by UV crosslinking assay
<p><b>Copyright information:</b></p><p>Taken from "Binding of pRNA to the N-terminal 14 amino acids of connector protein of bacteriophage phi29"</p><p>Nucleic Acids Research 2005;33(8):2640-2649.</p><p>Published online 10 May 2005</p><p>PMCID:PMC1092275.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> Lanes AâD, 10% SDSâPAGE autoradiographed pictures; lanes aâd, the same gel stained by Coomassie blue. Lane A, pRNA crosslinked to connector and then treated by RNase A; lane B, procapsid crosslinked to pRNA and then treated by RNase A; lane C, procapsidâpRNA complex without crosslinking, and then treated by RNase A; lane D, pRNA treated by RNase A. Lanes a, b, c and d correspond to A, B, C and D, respectively
A total of 10% SDSâPAGE to show the connector protein gp10 and procapsid treated before and after V8 cleavage
<p><b>Copyright information:</b></p><p>Taken from "Binding of pRNA to the N-terminal 14 amino acids of connector protein of bacteriophage phi29"</p><p>Nucleic Acids Research 2005;33(8):2640-2649.</p><p>Published online 10 May 2005</p><p>PMCID:PMC1092275.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> Lane 1, procapsid alone; lane 2, procapsid cleaved by proteaseV8; lane 3, purified connector protein gp10 alone; lane 4, connector cleaved by V8; lane 5, connectorâpRNA complex cleaved by V8. As noted in the text, in the procapsid, the C-terminus of gp10 is located at the wider end of the connector that is buried within the procapsid, while the N-terminus is located at the narrow end of the connector that is exposed to the solvent. Treatment of connector or procapsid with V8 resulted in different sizes of gp10, since V8 can cleave both the N- and C-terminus of gp10 of the free connector, but only the N-terminus of the gp10 that is buried within the procapsid. Gp8 is the capsid protein, while gp8.5 is the fiber protein of the procapsid
Enhancement of solubility in <it>Escherichia coli </it>and purification of an aminotransferase from <it>Sphingopyxis </it>sp. MTA144 for deamination of hydrolyzed fumonisin B<sub>1</sub>
Abstract Background Fumonisin B1 is a cancerogenic mycotoxin produced by Fusarium verticillioides and other fungi. Sphingopyxis sp. MTA144 can degrade fumonisin B1, and a key enzyme in the catabolic pathway is an aminotransferase which removes the C2-amino group from hydrolyzed fumonisin B1. In order to study this aminotransferase with respect to a possible future application in enzymatic fumonisin detoxification, we attempted expression of the corresponding fumI gene in E. coli and purification of the enzyme. Since the aminotransferase initially accumulated in inclusion bodies, we compared the effects of induction level, host strain, expression temperature, solubility enhancers and a fusion partner on enzyme solubility and activity. Results When expressed from a T7 promoter at 30°C, the aminotransferase accumulated invariably in inclusion bodies in DE3 lysogens of the E. coli strains BL21, HMS174, Rosetta 2, Origami 2, or Rosetta-gami. Omission of the isopropyl-beta-D-thiogalactopyranoside (IPTG) used for induction caused a reduction of expression level, but no enhancement of solubility. Likewise, protein production but not solubility correlated with the IPTG concentration in E. coli Tuner(DE3). Addition of the solubility enhancers betaine and sorbitol or the co-enzyme pyridoxal phosphate showed no effect. Maltose-binding protein, used as an N-terminal fusion partner, promoted solubility at 30°C or less, but not at 37°C. Low enzyme activity and subsequent aggregation in the course of purification and cleavage indicated that the soluble fusion protein contained incorrectly folded aminotransferase. Expression in E. coli ArcticExpress(DE3), which co-expresses two cold-adapted chaperonins, at 11°C finally resulted in production of appreciable amounts of active enzyme. Since His tag-mediated affinity purification from this strain was hindered by co-elution of chaperonin, two steps of chromatography with optimized imidazole concentration in the binding buffer were performed to obtain 1.45 mg of apparently homogeneous aminotransferase per liter of expression culture. Conclusions We found that only reduction of temperature, but not reduction of expression level or fusion to maltose-binding protein helped to produce correctly folded, active aminotransferase FumI in E. coli. Our results may provide a starting point for soluble expression of related aminotransferases or other aggregation-prone proteins in E. coli.</p