Ant-associated fungi in Bulgaria

The current study gives an overview of the ant-associated fungi in Bulgaria. After the recent first records for the country of Myrmicinosporidium durum Hölldobler, 1933, Rickia wasmannii Cavara, 1899, and Laboulbenia camponoti S.W.T. Batra, 1963, new data on their host range and distribution have been obtained. Additionally the ectoparasitic fungus Aegeritella tuberculata Bałazy & Wiśniewski, 1982, which has not been previously recorded from the Balkan Peninsula was found on the host ant Lasius brunneus (Latreille, 1798) in the Bulgarian part of Strandzha Mountain, in a locality near the border of Turkey.peer-reviewe

Chapter Life Cycle Assessment as a Tool to Implement Sustainable Development in the Bioeconomy and Circular Economy

In this chapter, the life cycle assessment was presented as a tool to implement sustainable development in the bioeconomy and circular economy. Bulky waste includes large items such as furniture, doors, flooring and mattresses. The management of bulky waste is a serious problem for European countries. The URBANREC project proposed a solution to this problem through the use of new technologies for the bulky waste processing. The aim of the URBANREC project is to implement an eco-innovative, integrated system of bulky waste management and demonstrate its effectiveness in various regions of Europe. The project has received funding from the European Union. In this chapter, the LCA environmental analysis was performed for the technology of grinding bulky waste using a water jet by the Ecofrag company. The calculations were carried out using SimaPro 8.5.2.0. The LCA analysis shows that the reuse of foams and mattresses contributes to the avoidance of their targeted production, which is related with the reduction of greenhouse gas emission and consumption of fossil raw materials

Life Cycle Assessment as a Tool to Implement Sustainable Development in the Bioeconomy and Circular Economy

In this chapter, the life cycle assessment was presented as a tool to implement sustainable development in the bioeconomy and circular economy. Bulky waste includes large items such as furniture, doors, flooring and mattresses. The management of bulky waste is a serious problem for European countries. The URBANREC project proposed a solution to this problem through the use of new technologies for the bulky waste processing. The aim of the URBANREC project is to implement an eco-innovative, integrated system of bulky waste management and demonstrate its effectiveness in various regions of Europe. The project has received funding from the European Union. In this chapter, the LCA environmental analysis was performed for the technology of grinding bulky waste using a water jet by the Ecofrag company. The calculations were carried out using SimaPro 8.5.2.0. The LCA analysis shows that the reuse of foams and mattresses contributes to the avoidance of their targeted production, which is related with the reduction of greenhouse gas emission and consumption of fossil raw materials

Bringing the Laboulbeniales to the 21st century: Enhanced techniques for extraction and PCR amplification of DNA from minute ectoparasitic fungi

One of the most peculiar groups of Ascomycota are the Laboulbeniales. These fungi are characterized by an ectoparasitic life style on arthropods, determinate growth, lack of an asexual stage, high species richness, and intractability to culture. The Laboulbeniales, sister to the Pyxidiophorales, have only recently been assigned their own class, the Laboulbeniomycetes, based on very few ribosomal DNA sequences. So far, DNA isolations and PCR amplifications have proven difficult. Here, we provide details of isolation techniques and the application of commercially available kits that enable efficient and reliable genetic analyses of these peculiar fungi. We provide 43 newly generated Laboulbeniales rDNA sequences, among which are the first published sequences for species in the genera Gloeandromyces, Herpomyces, Laboulbenia, Monoicomyces, and Polyandromyces. DNA extractions were possible using from 1 to 30 thalli from hosts preserved in ethanol (80-100%). In two cases, we successfully isolated DNA from thalli on dried insect collections. Laboulbeniales molecular systematics could be substantially enhanced through these improved methods by allowing more complete sampling of both taxa and gene regions

Antistaphylococcal Activity of Selected Thiourea Derivatives

Five of thiourea derivatives were prepared using as a starting compound 3-(trifluoromethyl)aniline, 4-chloro-3-nitroaniline, 1,3-thiazol-2-amine, 2H-1,2,3-triazol-4-amine and commercial isothiocyanates. All compounds were evaluated in vitro for antimicrobial activity. Derivatives 2 and 3 showed the highest inhibition against Gram-positive cocci (standard and hospital strains). The observed MIC values were in the range of 0.5–8 μg/ml. The products effectively inhibited the formation of biofilms of methicillin-resistant and standard strains of Staphylococcus epidermidis. Inhibitory activity of thioureas 2 and 3 against Staphylococcus aureus topoisomerase IV was studied. The examined compounds were nongenotoxic

Utilization of 4-n-nonylphenol by Metarhizium sp. isolates*

Nonylphenol (4-NP) is a xenobiotic classified as an endocrine disrupting compound with an ability to interfere with hormonal systems of numerous organisms including humans. It is widely distributed not only in aquatic but also in terrestrial systems. The aim of this study was to evaluate the ability of cosmopolitan fungus Metarhizium (commonly persistent in soil as a facultative insect pathogen, controlling populations of arthropods in natural environment) to degrade 4-n-nonylphenol. All isolates examined in this work were identified to a species rank based on five, independent genetic markers. Among eight Metarhizium strains; six of them have been identified as M. robertsii, and two others as M. brunneum and M. lepidiotae. All investigated Metarhzium isolates were found to eliminate 4-n-NP with significant efficiency (initial xenobiotic concentration 50 mg L -1 ). The degradation process was very effective and at 24h of incubation 50-90% of 4-n-NP was eliminated by certain strains, while extended incubation resulted in further utilization of this compound. At the end of the experiments 64-99% of 4-n-NP was removed from the culture medium. Additionally, in all tested cultures three major metabolites were detected: 4-hydroxybenzoic acid; 2-(4-hydroxyphenyl)acetic acid and 4-hydroxyphenylpentanoic acid. The obtained results indicate that Metarhizium sp. possesses an ability to degrade NP and can serve as a potential candidate for further biodegradation studies

Evaluation of the occurrence of canine congenital sensorineural deafness in puppies of predisposed dog breeds using the brainstem auditory evoked response

Canine congenital sensorineural deafness (CCSD) affects predisposed breeds of dogs and is primarily caused by an atrophy of the stria vascularis of the organ of Corti. The analysis of the brainstem auditory evoked response (BAER) is a reliable method for the evaluation of hearing in animals as it allows an accurate detection of unilateral or bilateral deafness. The occurrence of unilateral and bilateral deafness using the BAER was determined in a representative group of dogs in Poland, including Bull Terriers (n = 117), Australian Cattle Dogs (n = 62), English Setters (n = 32) and the Dogo Argentino (n = 32). Overall deafness, deafness in each dog breed and an association between deafness and phenotype were studied. Among the 243 dogs tested, 156 (81%) had a normal BAER, 27 (11%) were unilaterally deaf, and 12 (5%) were bilaterally deaf. The amplitudes and latencies of waves I, II, III, V, the V/I wave amplitude ratio, and wave I–V, I–III and III–V inter-peak intervals were recorded for each dog. Unilaterally and bilaterally deaf dogs were present in all the dog breeds studied. There were 17 (14.5%) deaf Bull Terriers, three (4.8%) deaf Australian Cattle Dogs, seven (21.9%) deaf English Setters, and 12 (37.5%) deaf Dogos Argentinos. Preventive BAER screening should be routinely performed in these four breeds to prevent the spread of genes responsible for deafness