A homological characterization of topological amenability

Generalizing Block and Weinberger’s characterization of amenability we introduce the notion of uniformly finite homology for a group action on a compact space and use it to give a homological characterization of topological amenability for actions. By considering the case of the natural action of G on its Stone-Cech compactification we obtain a homological characterisation of exactness of the group

Galactic Plane Hα\alpha Surveys: IPHAS & VPHAS+

The optical Galactic Plane H$\alpha$ surveys IPHAS and VPHAS+ are dramatically improving our understanding of Galactic stellar populations and stellar evolution by providing large samples of stars in short lived, but important, evolutionary phases, and high quality homogeneous photometry and images over the entire Galactic Plane. Here I summarise some of the contributions these surveys have already made to our understanding of a number of key areas of stellar and Galactic astronomy.Comment: 5 pages, 2 figures, refereed proceeding of the "The Universe of Digital Sky Surveys" conference, November 2014, to be published in the Astrophysics and Space Science Proceeding

Multiplex evaluation of influenza neutralizing antibodies with potential applicability to in-field serological studies

The increased number of outbreaks of H5 and H7 LPAI and HPAI viruses in poultry has major public and animal health implications. The continuous rapid evolution of these subtypes and the emergence of new variants influence the ability to undertake effective surveillance. Retroviral pseudotypes bearing influenza haemagglutinin (HA) and neuraminidase (NA) envelope glycoproteins represent a flexible platform for sensitive, readily standardized influenza serological assays. We describe a multiplex assay for the study of neutralizing antibodies that are directed against both influenza H5 and H7 HA. This assay permits the measurement of neutralizing antibody responses against two antigenically distinct HAs in the same serum/plasma sample thus increasing the amount and quality of serological data that can be acquired from valuable sera. Sera obtained from chickens vaccinated with a monovalent H5N2 vaccine, chickens vaccinated with a bivalent H7N1/H5N9 vaccine, or turkeys naturally infected with an H7N3 virus were evaluated in this assay and the results correlated strongly with data obtained by HI assay. We show that pseudotypes are highly stable under basic cold-chain storage conditions and following multiple rounds of freeze-thaw. We propose that this robust assay may have practical utility for in-field sero-surveillance and vaccine studies in resource-limited regions worldwide

Kinematic signatures of cluster formation from cool collapse in the Lagoon Nebula cluster NGC 6530

We examine the mass dependence of the velocity dispersion of stars in the young cluster NGC 6530 to better understand how it formed. Using a large sample of members we find that the proper motion velocity dispersion increases with stellar mass. While this trend is the opposite of that predicted if the cluster were developing energy equipartition, it is in agreement with recent N-body simulations that find such a trend develops because of the Spitzer instability. In these simulations the massive stars sink to the centre of the cluster and form a self-gravitating system with a higher velocity dispersion. If the cluster has formed by the cool collapse of an initially substructured distribution, then this occurs within 1–2 Myr, in agreement with our observations of NGC 6530. We therefore conclude that NGC 6530 formed from much more extended initial conditions and has since collapsed to form the cluster we see now. This cluster formation model is inconsistent with the idea that all stars form in dense, compact clusters and provides the first dynamical evidence that star clusters can form by hierarchical mergers between subclusters

Evolution of foot-and-mouth disease virus intra-sample sequence diversity during serial transmission in bovine hosts

RNA virus populations within samples are highly heterogeneous, containing a large number of minority sequence variants which can potentially be transmitted to other susceptible hosts. Consequently, consensus genome sequences provide an incomplete picture of the within- and between-host viral evolutionary dynamics during transmission. Foot-and-mouth disease virus (FMDV) is an RNA virus that can spread from primary sites of replication, via the systemic circulation, to found distinct sites of local infection at epithelial surfaces. Viral evolution in these different tissues occurs independently, each of them potentially providing a source of virus to seed subsequent transmission events. This study employed the Illumina Genome Analyzer platform to sequence 18 FMDV samples collected from a chain of sequentially infected cattle. These data generated snap-shots of the evolving viral population structures within different animals and tissues. Analyses of the mutation spectra revealed polymorphisms at frequencies >0.5% at between 21 and 146 sites across the genome for these samples, while 13 sites acquired mutations in excess of consensus frequency (50%). Analysis of polymorphism frequency revealed that a number of minority variants were transmitted during host-to-host infection events, while the size of the intra-host founder populations appeared to be smaller. These data indicate that viral population complexity is influenced by small intra-host bottlenecks and relatively large inter-host bottlenecks. The dynamics of minority variants are consistent with the actions of genetic drift rather than strong selection. These results provide novel insights into the evolution of FMDV that can be applied to reconstruct both intra- and inter-host transmission routes

Mass segregation in star clusters is not energy equipartition

Mass segregation in star clusters is often thought to indicate the onset of energy equipartition, where the most massive stars impart kinetic energy to the lower-mass stars and brown dwarfs/free floating planets. The predicted net result of this is that the centrally concentrated massive stars should have significantly lower velocities than fast-moving low-mass objects on the periphery of the cluster. We search for energy equipartition in initially spatially and kinematically substructured N-body simulations of star clusters with N = 1500 stars, evolved for 100 Myr. In clusters that show significant mass segregation we find no differences in the proper motions or radial velocities as a function of mass. The kinetic energies of all stars decrease as the clusters relax, but the kinetic energies of the most massive stars do not decrease faster than those of lower-mass stars. These results suggest that dynamical mass segregation -- which is observed in many star clusters -- is not a signature of energy equipartition from two-body relaxation

Evolution of spatio-kinematic structures in star-forming regions: are Friends of Friends worth knowing?

The Friends of Friends algorithm identifies groups of objects with similar spatial and kinematic properties, and has recently been used extensively to quantify the distributions of gas and stars in young star-forming regions. We apply the Friends of Friends algorithm to N-body simulations of the dynamical evolution of subvirial (collapsing) and supervirial (expanding) star-forming regions. We find that the algorithm picks out a wide range of groups (1–25) for statistically identical initial conditions, and cannot distinguish between subvirial and supervirial regions in that we obtain similar mode and median values for the number of groups it identifies. We find no correlation between the number of groups identified initially and either the initial or subsequent spatial and kinematic tracers of the regions’ evolution, such as the amount of spatial substructure, dynamical mass segregation, or velocity dispersion. We therefore urge caution in using the Friends of Friends algorithm to quantify the initial conditions of star formation

The Spectral and Temporal Properties of Transient Sources in Early-type Galaxies

Copyright 2012 Elsevier B.V., All rights reserved.We report the spectral and temporal variability properties of 18 candidate transient (TC) and potential transient (PTC) sources detected in deep multi-epoch Chandra observation of the nearby elliptical galaxies, NGC3379, NGC4278, and NGC4697. Only one source can be identified with a background counterpart, leaving 17 TCs + PTCs in the galaxies. Of these, 14 are in the galaxy field, supporting the theoretical picture that the majority of field X-ray binaries (XRBs) will exhibit transient accretion for >75% of their lifetime. Three sources are coincident with globular clusters, including two high-luminosity candidate black hole (BH) XRBs, with Lx= 5.4 × 10 erg/s and Lx= 2.8 × 10 erg/s, respectively. The spectra, luminosities, and temporal behavior of these 17 sources suggest that the transient population is heterogeneous, including neutron star (NS) and BH XRBs in both normal and high-rate accretion modes, and super soft sources containing white dwarf binaries. Our TC and PTC detections are noticeably fewer than the number expected from the population synthesis (PS) models of Fragos et al., tailored to our new Chandra pointings of NGC4278. We attribute this discrepancy to the PS assumption that the transient population is composed of NS XRBs, as well as differences between the statistical analysis and error estimates used in the model and our observationsPeer reviewe

Bat and pig IFN-induced transmembrane protein 3 restrict cell entry by influenza virus and lyssaviruses

IFN-induced transmembrane protein 3 (IFITM3) is a restriction factor that blocks cytosolic entry of numerous viruses that utilize acidic endosomal entry pathways. In humans and mice, IFITM3 limits influenza-induced morbidity and mortality. Although many IFITM3-sensitive viruses are zoonotic, whether IFITMs function as antiviral restriction factors in mammalian species other than humans and mice is unknown. Here, IFITM3 orthologues in the microbat (Myotis myotis) and pig (Sus scrofa domesticus) were identified using rapid amplification of cDNA ends. Amino acid residues known to be important for IFITM3 function were conserved in the pig and microbat orthologues. Ectopically expressed pig and microbat IFITM3 co-localized with transferrin (early endosomes) and CD63 (late endosomes/multivesicular bodies). Pig and microbat IFITM3 restricted cell entry mediated by multiple influenza haemagglutinin subtypes and lyssavirus glycoproteins. Expression of pig or microbat IFITM3 in A549 cells reduced influenza virus yields and nucleoprotein expression. Conversely, small interfering RNA knockdown of IFITM3 in pig NPTr cells and primary microbat cells enhanced virus replication, demonstrating that these genes are functional in their species of origin at endogenous levels. In summary, we showed that IFITMs function as potent broad-spectrum antiviral effectors in two mammals – pigs and bats – identified as major reservoirs for emerging viruses

First results of an Hα based search of classical Be stars in the Perseus Arm and beyond

We investigate a region of the Galactic plane, between 120° ≤ l ≤ 140° and-1° ≤ b≤+4°, and uncover a population of moderately reddened (E(B-V) ~ 1) classical Be stars within and beyond the Perseus and Outer Arms. 370 candidate emission-line stars (13≲r≲16) selected from the Isaac Newton Telescope Photometric Ha Survey of the Northern Galactic plane have been followed up spectroscopically. A subset of these, 67 stars with properties consistent with those of classical Be stars, have been observed at sufficient spectral resolution (δλ ≈ 2-4 Å) at blue wavelengths to narrow down their spectral types. We determine these to a precision estimated to be ±1 subtype and then we measure reddenings via spectral energy distribution fitting with reference to appropriate model atmospheres. Corrections for contribution to colour excess from circumstellar discs are made using an established scaling to Ha emission equivalent width. Spectroscopic parallaxes are obtained after luminosity class has been constrained via estimates of distances to neighbouring A/F stars with similar reddenings. Overwhelmingly, the stars in the sample are confirmed as luminous classical Be stars at heliocentric distances ranging from 2 kpc up to ~12 kpc. However, the errors are presently too large to enable the cumulative distribution function with respect to distance to distinguish between models placing the stars exclusively in spiral arms, or in a smooth exponentially declining distribution.Peer reviewe