Using individual tracking data to validate the predictions of species distribution models

The authors would like to thank the College of Life Sciences of Aberdeen University and Marine Scotland Science which funded CP's PhD project. Skate tagging experiments were undertaken as part of Scottish Government project SP004. We thank Ian Burrett for help in catching the fish and the other fishermen and anglers who returned tags. We thank José Manuel Gonzalez-Irusta for extracting and making available the environmental layers used as environmental covariates in the environmental suitability modelling procedure. We also thank Jason Matthiopoulos for insightful suggestions on habitat utilization metrics as well as Stephen C.F. Palmer, and three anonymous reviewers for useful suggestions to improve the clarity and quality of the manuscript.Peer reviewedPostprintPostprintPostprintPostprintPostprin

High-Resolution Identification of Multiple Salmonella Serovars in a Single Sample by Using CRISPR-SeroSeq

Salmonella enterica is represented by \u3e2,600 serovars that can differ in routes of transmission, host colonization, and in resistance to antimicrobials. S. enterica is the leading bacterial cause of foodborne illness in the United States, with well-established detection methodology. Current surveillance protocols rely on the characterization of a few colonies to represent an entire sample; thus, minority serovars remain undetected. Salmonella contains two CRISPR loci, CRISPR1 and CRISPR2, and the spacer contents of these can be considered serovar specific. We exploited this property to develop an amplicon-based and multiplexed sequencing approach, CRISPR-SeroSeq (serotyping by sequencing of the CRISPR loci), to identify multiple serovars present in a single sample. Using mixed genomic DNA from two Salmonella serovars, we were able to confidently detect a serovar that constituted 0.01% of the sample. Poultry is a major reservoir of Salmonella spp., including serovars that are frequently associated with human illness, as well as those that are not. Numerous studies have examined the prevalence and diversity of Salmonella spp. in poultry, though these studies were limited to culture-based approaches and therefore only identified abundant serovars. CRISPR-SeroSeq was used to investigate samples from broiler houses and a processing facility. Ninety-one percent of samples harbored multiple serovars, and there was one sample in which four different serovars were detected. In another sample, reads for the minority serovar comprised 0.003% of the total number of Salmonella spacer reads. The most abundant serovars identified were Salmonella enterica serovars Montevideo, Kentucky, Enteritidis, and Typhimurium. CRISPR-SeroSeq also differentiated between multiple strains of some serovars. This high resolution of serovar populations has the potential to be utilized as a powerful tool in the surveillance of Salmonella species

The TRENDS High-Contrast Imaging Survey. V. Discovery of an Old and Cold Benchmark T-dwarf Orbiting the Nearby G-star HD 19467

The nearby Sun-like star HD 19467 shows a subtle radial velocity (RV) acceleration of -1.37+/-0.09 m/s/yr over an 16.9 year time baseline (an RV trend), hinting at the existence of a distant orbiting companion. We have obtained high-contrast adaptive optics images of the star using NIRC2 at Keck Observatory and report the direct detection of the body that causes the acceleration. The companion, HD 19467 B, is dK=12.57+/-0.09 mag fainter than its parent star (contrast ratio of 9.4e-6), has blue colors J-K_s=-0.36+/-0.14 (J-H=-0.29+/-0.15), and is separated by 1.653+/-0.004" (51.1+/-1.0 AU). Follow-up astrometric measurements obtained over an 1.1 year time baseline demonstrate physical association through common parallactic and proper motion. We calculate a firm lower-limit of m>51.9^{+3.6}_{-4.3}Mjup for the companion mass from orbital dynamics using a combination of Doppler observations and imaging. We estimate a model-dependent mass of m=56.7^{+4.6}_{-7.2}Mjup from a gyrochronological age of 4.3^{+1.0}_{-1.2} Gyr. Isochronal analysis suggests a much older age of $9\pm1$ Gyr, which corresponds to a mass of m=67.4^{+0.9}_{-1.5}Mjup. HD 19467 B's measured colors and absolute magnitude are consistent with a late T-dwarf [~T5-T7]. We may infer a low metallicity of [Fe/H]=-0.15+/-0.04 for the companion from its G3V parent star. HD 19467 B is the first directly imaged benchmark T-dwarf found orbiting a Sun-like star with a measured RV acceleration.Comment: Updated to reflect ApJ versio

SOCIAL MEDIA: CREATING STUDENT AWARENESS OF ITS USE IN THE HIRING PROCESS

As the use of social media permeates our lives, it is important for business educators to promote the effective use of this technology to students for both their role as job seekers as well as potential hiring managers. This article will present current perceptions among business students on using social media in the job search process, primary research from recruiters in an attempt to understand employers’ policies and practices with respect to the use of social media in the hiring process, key laws which students should be aware of with respect to the use of social media by employers, and practical guidance for students throughout their business school experience. In addition, a proposed teaching method will be presented on how to convey this practical guidance to students in a compelling and impactful manner

Kinetics of phase-separation in the critical spherical model and local scale-invariance

The scaling forms of the space- and time-dependent two-time correlation and response functions are calculated for the kinetic spherical model with a conserved order-parameter and quenched to its critical point from a completely disordered initial state. The stochastic Langevin equation can be split into a noise part and into a deterministic part which has local scale-transformations with a dynamical exponent z=4 as a dynamical symmetry. An exact reduction formula allows to express any physical average in terms of averages calculable from the deterministic part alone. The exact spherical model results are shown to agree with these predictions of local scale-invariance. The results also include kinetic growth with mass conservation as described by the Mullins-Herring equation.Comment: Latex2e with IOP macros, 28 pp, 2 figures, final for

The Dynamical Mass and Three-Dimensional Orbit of HR7672B: A Benchmark Brown Dwarf with High Eccentricity

The companion to the G0V star HR7672 directly imaged by Liu et al. (2002) has moved measurably along its orbit since the discovery epoch, making it possible to determine its dynamical properties. Originally targeted with adaptive optics because it showed a long-term radial velocity acceleration (trend), we have monitored this star with precise Doppler measurements and have now established a 24 year time baseline. The radial velocity variations show significant curvature (change in the acceleration) including an inflection point. We have also obtained a recent image of HR7672B with NIRC2 at Keck. The astrometry also shows curvature. In this paper, we use jointly-fitted Doppler and astrometric models to calculate the three-dimensional orbit and dynamical mass of the companion. The mass of the host star is determined using a direct radius measurement from CHARA interferometry in combination with high resolution spectroscopic modeling. We find that HR7672B has a highly eccentric, $e=0.50^{+0.01}_{-0.01}$, near edge-on, $i=97.3^{+0.4}_{-0.5}$ deg, orbit with semimajor axis, $a=18.3^{+0.4}_{-0.5}$ AU. The mass of the companion is $m=68.7^{+2.4}_{-3.1}M_J$ at the 68.2% confidence level. HR7672B thus resides near the substellar boundary, just below the hydrogen-fusing limit. These measurements of the companion mass are independent of its brightness and spectrum and establish HR7672B as a rare and precious "benchmark" brown dwarf with a well-determined mass, age, and metallicity essential for testing theoretical evolutionary models and synthetic spectral models. It is presently the only directly imaged L,T,Y-dwarf known to produce an RV trend around a solar-type star.Comment: accepted to Ap

Human Impacts Flatten Rainforest-Savanna Gradient and Reduce Adaptive Diversity in a Rainforest Bird

Ecological gradients have long been recognized as important regions for diversification and speciation. However, little attention has been paid to the evolutionary consequences or conservation implications of human activities that fundamentally change the environmental features of such gradients. Here we show that recent deforestation in West Africa has homogenized the rainforest-savanna gradient, causing a loss of adaptive phenotypic diversity in a common rainforest bird, the little greenbul (Andropadus virens). Previously, this species was shown to exhibit morphological and song divergence along this gradient in Central Africa. Using satellite-based estimates of forest cover, recent morphological data, and historical data from museum specimens collected prior to widespread deforestation, we show that the gradient has become shallower in West Africa and that A. virens populations there have lost morphological variation in traits important to fitness. In contrast, we find no loss of morphological variation in Central Africa where there has been less deforestation and gradients have remained more intact. While rainforest deforestation is a leading cause of species extinction, the potential of deforestation to flatten gradients and inhibit rainforest diversification has not been previously recognized. More deforestation will likely lead to further flattening of the gradient and loss of diversity, and may limit the ability of species to persist under future environmental conditions

Bioinvasion in a Brazilian Bay: Filling Gaps in the Knowledge of Southwestern Atlantic Biota

Biological invasions are a major cause of global species change. Nevertheless, knowledge about the distribution and ecology of introduced species is regionally biased, and many gaps in knowledge exist for most developing countries.To study the zoobenthos on the hard substratum of the Ilha Grande Bay, a survey was conducted on both natural and artificial substrata at three depths and seven sites. The species recorded were classified as native, cryptogenic or introduced. Multivariate analyses were conducted to assess the prevalence of introduced species in these communities and to compare the distribution of species on natural and artificial substrata of this bay to identify possible discrepancies in habitat use. Of the 61 species, 25 were cryptogenic, 10 were introduced and 26 were native. Similar numbers of introduced species were found on both natural and artificial substrata, though the community composition was significantly different between them. We also compared the species composition of the Ilha Grande Bay survey to other inventories taken around the world. The highest similarities were found between the Ilha Grande Bay inventory and the Atlantic coastal region (Tampa Bay, USA and the Gulf of Mexico), American Samoa and Pearl Harbor (USA) inventories.This study presents the first published comprehensive list of hard substratum sessile marine invertebrate species in a Brazilian bay. The high percentage of cryptogenic species reveals gaps in both zoological records and information on introduced species for the Brazilian coast. The introduced species successfully colonized different sites in the Ilha Grande Bay, including both natural and artificial substrata. In addition, we find that artificial structures may not be good surrogates for natural rocky shores and may represent an ecological threat. Comparisons with other inventories suggest a history of broad-scale invasion, though more evidence is needed to support this conclusion

CemOrange2 fusions facilitate multifluorophore subcellular imaging in C. elegans

Due to its ease of genetic manipulation and transparency, Caenorhabditis elegans (C. elegans) has become a preferred model system to study gene function by microscopy. The use of Aequorea victoria green fluorescent protein (GFP) fused to proteins or targeting sequences of interest, further expanded upon the utility of C. elegans by labeling subcellular structures, which enables following their disposition during development or in the presence of genetic mutations. Fluorescent proteins with excitation and emission spectra different from that of GFP accelerated the use of multifluorophore imaging in real time. We have expanded the repertoire of fluorescent proteins for use in C. elegans by developing a codon-optimized version of Orange2 (CemOrange2). Proteins or targeting motifs fused to CemOrange2 were distinguishable from the more common fluorophores used in the nematode; such as GFP, YFP, and mKate2. We generated a panel of CemOrange2 fusion constructs, and confirmed they were targeted to their correct subcellular addresses by colocalization with independent markers. To demonstrate the potential usefulness of this new panel of fluorescent protein markers, we showed that CemOrange2 fusion proteins could be used to: 1) monitor biological pathways, 2) multiplex with other fluorescent proteins to determine colocalization and 3) gain phenotypic knowledge of a human ABCA3 orthologue, ABT-4, trafficking variant in the C. elegans model organism

Field Evaluation of Commercially Available Small Unmanned Aircraft Crop Spray Systems

Agricultural research and development on small unmanned aircraft systems (UAS) has been directed toward UAS enabled sensing to detect features of interest. While compelling, there is an immediate need to increase the breadth and depth of UAS-based research, to move beyond sensing, and explore active intervention in agricultural production systems. This paper is focused on the concept of crop protection through ultra-precise, unmanned aerial application systems, and seeks to initiate research discussion in this important area of opportunity. Toward this end, two different, commercially available, small Unmanned Aerial Application Systems (sUAAS - defined as less than 55 lbs. maximum take-off weight) were evaluated for operational techniques and application system efficacy under dynamic field conditions. The performance of the factory supplied spray equipment systems are documented using traditional aerial spray testing methods that have been modified for UAS enabled application systems, referred to as small Unmanned Aerial Application Systems (sUAAS). Results from initial testing protocols indicate that the factory supplied systems are quite different in design and implementation, with spray test results that reflect this difference in design, in both deposition and spray swath. Further, it is apparent that with the advent of unmanned aerial application systems, and the unique characteristics of the integrated aircraft and application systems, there is a very real need for the development of standardized sUAAS testing procedures