Eccentrically Induced Skeletal Muscle Damage in Patients With Chronic Fatigue Syndrome (CFS), With Reference to Overtrained Athletes

Chronic fatigue syndrome (CFS) and Overtraining syndrome (OTS) are separate, complex conditions which have so many similar debilitating effects that it has led some researchers to conclude that OTS is a sub-condition of CFS. The purpose of this research was to compare the force and damage-recovery characteristics of skeletal muscle in CFS patients and control normals, after a single damaging bout of eccentric contractions in the non-dominant forearm flexors. The subjects ( n = 25 ), a convenience sample were assigned to three groups; [I] CFS + eccentric damage (n = 8), [2] Control Damage (CD)+ eccentric damage (n = 10), and [3] Control (ND) + no damage (n = 7). The research was carried out over a four week period using the following format. CFS & CD groups received eccentrically induced muscle damage of the forearm flexors by 35 isokinetic eccentric (7 x 5, 2 minutes recovery between sets) contractions at 90° sec-1 with the forearm returning passively at 15°sec-1 Testing was undertaken pre-damage and I, 2, 4, 6, 8, 12, 16, 20, 24, & 28 days post-damage, by measurements of voluntary maximal concentric isokinetic force at 150°sec-1, isometric maximal voluntary contraction at approximately 90° elbow flexion, electrically stimulated 20 : 50Hz isometric force ratio at approximately 90° elbow flexion, muscle pain, and blood CK. Groups were compared on these variables using Students independent t-test and repeated measures two way ANOVA with simple contrasts. Alpha was set at 0.05 level. The results of this study were significant for the eccentric force produced in the damage bout with the CFS group producing less force after the 4th set (p \u3c 0.05). Serum CK concentration, which following eccentric damage was significantly higher in the CFS group than the CD group (p \u3c 0.01), and the ND group (Q \u3c 0.001). The low frequency fatigue (LFF) ratio was significantly lower in the CFS group 2, 4, 6 & 8 days post-damage when compared to the CD group. Maximal isometric voluntary force and isokinetic concentric peak torque (PT) & average peak torque (AT) loss was significantly greater in the CFS group compared to the CD group (isometric 12 \u3c 0.01, PT 12 \u3c 0.01; & AT Q \u3c 0.001) and ND group (isometric TI \u3c 0.01; PT TI \u3c 0.001; & AT 12 \u3c 0.001 ). The intensity of delayed onset muscle soreness (DOMS) was significantly less 6 days post-damage in the CFS group, when compared to the CD group (TI \u3c 0.05). The combination of an increased CK efflux and low frequency fatigue, that is of both greater depth and longer lasting, together with greater isometric and concentric force losses, indicates that the subjects with CFS have a lower threshold for muscular damage, that is more profound and slower to recover than in healthy individuals

Zebrafish Caudal Haematopoietic Embryonic Stromal Tissue (CHEST) Cells Support Haematopoiesis.

Haematopoiesis is an essential process in early vertebrate development that occurs in different distinct spatial locations in the embryo that shift over time. These different sites have distinct functions: in some anatomical locations specific hematopoietic stem and progenitor cells (HSPCs) are generated de novo. In others, HSPCs expand. HSPCs differentiate and renew in other locations, ensuring homeostatic maintenance. These niches primarily control haematopoiesis through a combination of cell-to-cell signalling and cytokine secretion that elicit unique biological effects in progenitors. To understand the molecular signals generated by these niches, we report the generation of caudal hematopoietic embryonic stromal tissue (CHEST) cells from 72-hours post fertilization (hpf) caudal hematopoietic tissue (CHT), the site of embryonic HSPC expansion in fish. CHEST cells are a primary cell line with perivascular endothelial properties that expand hematopoietic cells in vitro. Morphological and transcript analysis of these cultures indicates lymphoid, myeloid, and erythroid differentiation, indicating that CHEST cells are a useful tool for identifying molecular signals critical for HSPC proliferation and differentiation in the zebrafish. These findings permit comparison with other temporally and spatially distinct haematopoietic-supportive zebrafish niches, as well as with mammalian haematopoietic-supportive cells to further the understanding of the evolution of the vertebrate hematopoietic system

A field-deployable digital acoustic measurement system

A field deployable digital acoustic measurement system was developed to support acoustic research programs at the Langley Research Center. The system digitizes the acoustic inputs at the microphone, which can be located up to 1000 feet from the van which houses the acquisition, storage, and analysis equipment. Digitized data from up to 12 microphones is recorded on high density 8mm tape and is analyzed post-test by a microcomputer system. Synchronous and nonsynchronous sampling is available with maximum sample rates of 12,500 and 40,000 samples per second respectively. The high density tape storage system is capable of storing 5 gigabytes of data at transfer rates up to 1 megabyte per second. System overall dynamic range exceeds 83 dB

Revision of Earth-sized Kepler Planet Candidate Properties with High Resolution Imaging by Hubble Space Telescope

We present the results of our Hubble Space Telescope program and describe how our analysis methods were used to re-evaluate the habitability of some of the most interesting Kepler planet candidates. Our program observed 22 Kepler Object of Interest (KOI) host stars, several of which were found to be multiple star systems unresolved by Kepler. We use our high-resolution imaging to spatially resolve the stellar multiplicity of Kepler-296, KOI-2626, and KOI-3049, and develop a conversion to the Kepler photometry (Kp) from the F555W and F775W filters on WFC3/UVIS. The binary system Kepler-296 (5 planets) has a projected separation of 0.217" (80AU); KOI-2626 (1 planet candidate) is a triple star system with a projected separation of 0.201" (70AU) between the primary and secondary components and 0.161" (55AU) between the primary and tertiary; and the binary system KOI-3049 (1 planet candidate) has a projected separation of 0.464" (225AU). We use our measured photometry to fit the separated stellar components to the latest Victoria-Regina Stellar Models with synthetic photometry to conclude that the systems are coeval. The components of the three systems range from mid-K dwarf to mid-M dwarf spectral types. We solved for the planetary properties of each system analytically and via an MCMC algorithm using our independent stellar parameters. The planets range from ~1.6R_Earth to ~4.2R_Earth, mostly Super Earths and mini-Neptunes. As a result of the stellar multiplicity, some planets previously in the Habitable Zone are, in fact, not, and other planets may be habitable depending on their assumed stellar host.Comment: 16 pages, 10 figures, ApJ, 804, 9

Stand-alone digital data storage control system including user control interface

A storage control system includes an apparatus and method for user control of a storage interface to operate a storage medium to store data obtained by a real-time data acquisition system. Digital data received in serial format from the data acquisition system is first converted to a parallel format and then provided to the storage interface. The operation of the storage interface is controlled in accordance with instructions based on user control input from a user. Also, a user status output is displayed in accordance with storage data obtained from the storage interface. By allowing the user to control and monitor the operation of the storage interface, a stand-alone, user-controllable data storage system is provided for storing the digital data obtained by a real-time data acquisition system

The effect of within-crop habitat manipulations on the conservation biological control of aphids in field-grown lettuce

Within-crop habitat manipulations have the potential to increase the biological control of pests in horticultural field crops. Wildflower strips have been shown to increase the abundance of natural enemies, but there is little evidence to date of an impact on pest populations. The aim of this study was to determine whether withincrop wildflower strips can increase the natural regulation of pests in horticultural field crops. Aphid numbers in plots of lettuce grown adjacent to wildflower strips were compared with those in plots grown in the absence of wildflowers. The presence of wildflower strips led to a decrease in aphid numbers on adjacent lettuce plants during June and July, but had less impact in August and September. The decrease in aphid numbers was greatest close to the wildflower strips and, the decrease in aphid numbers declined with increasing distance from the wildflower strips, with little effect at a distance of ten metres. The main natural enemies found in the crop were those that dispersed aerially, which is consistent with data from previous studies on cereal crops. Analysis and interpretation of natural enemy numbers was difficult due to low recovery of natural enemies, and the numbers appeared to follow changes in aphid abundance rather than being directly linked to the presence of wildflower strips. Cutting the wildflower strips, to remove floral resources, had no impact on the reduction in aphid numbers achieved during June and July, but decreased the effect of the wildflower strips during August and September. The results suggest that wildflower strips can lead to increased natural regulation of pest aphids in outdoor lettuce crops, but more research is required to determine how this is mediated by natural enemies and how the impact of wildflower strips on natural pest regulation changes during the growing season

Military service and crime: new evidence

BACKGROUND: Evidence indicates that a substantial proportion of military personnel are involved in high-risk and antisocial behaviors that place them at jeopardy for criminal justice system involvement. However, prior research on military service and crime has disproportionately focused on veterans from the Vietnam War era (1955–1975), and has tended to focus on either current or former military members. METHODS: This study employed data from a population-based study (i.e., National Study on Drug Use and Health [NSDUH] between 2002 and 2014). It systematically examines the prevalence of self-reported antisocial behaviors, criminal justice system involvement, and substance abuse among the US civilian population and military service members, including reservists (n = 2206) and those who reported having been separated or retired from military service (n = 20,551). These factors are further examined across the developmental spectrum of adulthood (ages 18–34, 35–49, and 50–64). RESULTS: Results showed that military members were more prone to lifetime arrests and overall substance misuse. However, additional findings emerged suggesting that, while the military population overall seems to be positively associated with higher criminal activity than that found in the civilian population, these findings were based on a specific subgroup of the veteran population. This subgroup is comprised of individuals who likely did not fit in with the military culture and were discharged from the military early in their careers. CONCLUSION: Additional research on identifying this subgroup of military members is encouraged to better concentrate on prevention and treatment measures

Learning a keying sequence you never executed: Evidence for independent associative and motor chunk learning

A substantial amount of research has addressed how people learn and control movement sequences. Recent results suggested that practice with discrete key pressing sequences results in two types of sequence learning: associative learning and motor chunk development (Verwey & Abrahamse, 2012). In the present study, we addressed whether in keying sequences of limited length associative learning develops also when the use of the chunking mode is prevented by introducing during practice random deviants. In line with the notion of two different learning mechanisms, the present results indicate that associative sequence learning develops when motor chunks cannot be developed during practice. This confirms the notion that motor chunks do not rely on these associations. In addition, experience with a particular execution mode during the practice phase seems to benefit subsequent use of that mode with unfamiliar and random sequences. Also, participants with substantial video-gaming experience were faster in executing discrete keying sequences in the chunking mode. These last two results may point to the development of a general ability to produce movement sequences in the chunking mode

Metronome-Cued Stepping in Place after Hemiparetic Stroke: Comparison of a One- and Two-Tone Beat

Hemiparetic gait is characterised by temporal asymmetry and variability, and these variables are improved by auditory cueing. Stepping in place incorporates aspects of gait and may be a useful tool for locomotor training. The aim of this pilot study was to investigate the use of a single-tone and dual-tone metronome to cue stepping in place after hemiparetic stroke. Eight participants completed an uncued baseline stepping condition and two cued stepping conditions utilising a single-tone and a dualtone metronome. Step times were determined from force plate data, and asymmetry and variability were calculated for the three conditions. Step time asymmetry was significantly reduced in the single-tone condition compared to baseline, and paretic step time variability was significantly reduced in both cued conditions.The single-tone metronome appeared to be preferred to the dual-tone metronome based on participant feedback.The results of this pilot study suggest that metronome cueing produces similar benefits on stepping in place to previously reported findings in walking. Further research on whether stepping in place to a metronome can be used for locomotor training is needed