Kemampuan Yogurt Sinbiotik Berbasis Probiotik Lokal Dalam Mencegah Diare Dan Meningkatkan Imunitas Tikus Putih (Rattus Norvegicus)

Tujuan penelitian ini adalah mengamati kemampuan yogurt sinbiotik yang mengandung probiotik lokal (Lactobacillus acidophilus 2B4) sebagai antidiare pada tikus yang terinfeksi Enteropathogenic Escherichia coli (EPEC), dan untuk mendeteksi dampak terhadap karakteristik imunomodulator (jumlah sel limfosit, kadar malonaldehid/MDA hati, dan aktivitas antioksidan superoksida dismutase/SOD hati). Infeksi EPEC (10 7 cfu/ml/hari) yang dilakukan secara oral ke tikus selama tujuh hari berturut-turut secara nyata menyebabkan diare ringan tanpa penurunan berat badan. Pemberian secara oral yogurt sinbiotik yang mengandung 10 9 cfu bakteri asam laktat/ml/hari selama 21 hari secara nyata meningkatkan respons imun tikus, yang ditunjukkan dengan meningkatnya sel limfosit di hari ke-14, penurunan MDA hati pada hari ke 14 dan 21, dan meningkatnya aktivitas SOD hati pada hari ke-14

The Tuber Extract and Flour of Dioscorea Alatanormalize the Blood Lipid Profile of Rabbits Treated with High Cholesterol Diets

Background: Dioscorea alata(DA) tuber has potential to prevent the condition of hyperlipidemia due to the bioactive compound, such as anthocyanins, diosgenin, and dietary fiber that beneficial in normalizing blood lipid profiles. In this research, the effect of water extract and flour of DA tuber administration was examined on rabbits treated with high cholesterol diets.Methods:DA tuber extract and flour were administrated to the rabbits for 60 days using completely randomised design. The ration treatment are as follows: 1) Basal ration as negative control (K0), 2) Basal ration + 0.5% cholesterol, as positive control (K1), 3) Basal ration + 0.5% cholesterol + DA extract 1.8 g/100 g (KE1), 4) Basal ration + cholesterol 0.5% + DA extract 3.6 g/100 g (KE2), 5) Basal ration with 15% DA flour + 0.5% cholesterol (KT1) and 6) Basal ration with 30% DA flour + 0.5% cholesterol (KT2). The Total cholesterol, HDL, LDL cholesterol in serum were analysed at baseline, days 28, days 56 and at the end of study.Results:The administration of high cholesterol (1%) ration increased blood lipid levels by 16 fold compared to that of control. The administration of 15% and 30% of DA flour could maintain blood lipid profile to normal condition, in particular at 30% substitution DA flour. However the water extract of DA can not maintain a normal blood lipids of high cholesterol treated rabbitsConclusion: Dioscorea alata flour has suggested to have anti-hyperlipidemia effect. (Health Science Indones 2014;1:23-9