Welding 3D Printed Structures for Composite Sacrificial Tooling

3D printed parts offer the ability to generate dimensioned, complex objects with minimal machining touch time and skill, but they are typically very weak and limited in size. Strong and light composite parts require tooling to be created for the fabric to lay on while the resin is curing, but tooling can be quite expensive during the prototyping phase or for low part quantity runs. This study examined the techniques required to weld smaller 3D printed parts together to form large 3D printed tools that could be used as a sacrificial tool for a composite part. In this method, the 3D printed structure would remain inside the part and provide support and dimensional reference during the composite curing process. Friction, hot extrusion, and hot contact welding methods were examined using lap shear joints in both tensile and flexural test methods to determine effective joinery style and overlap lengths to achieve normal 3D printed properties. The results of this study demonstrate how multiple 3D printers could be used to create cost-effective rapid prototyping and low part quantity runs for composite structures by welding smaller 3D printed structures into a larger single 3D printed part

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Structural similarity of chymopapain forms as indicated by circular dichroism.

Four chymopapain forms were isolated by high-resolution liquid chromatography on a cation-exchange column. The three major forms possess nearly identical secondary and tertiary structures, as judged from their c.d. spectra; these components showed similar proteolytic activity and Mr values close to that of papain. The fourth isolated component seems to be a mixture of modified proteins

Legislative Documents

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Combined hourly acceleration values of Murray cod (<i>Maccullochella peelii</i>; n = 18) for each calendar month of the study.

<p>Data are represented as median, 25^th and 75^th percentiles (box) and 5^th and 95^th percentiles (whisker).</p

Summary information on individual Murray cod (<i>Maccullochella peelii</i>) fitted with accelerometer acoustic telemetry tags and monitored from January–June 2016.

<p>Summary information on individual Murray cod (<i>Maccullochella peelii</i>) fitted with accelerometer acoustic telemetry tags and monitored from January–June 2016.</p

Frequency histogram of Murray cod (<i>Maccullochella peelii</i>) acceleration values measured during the study.

<p>Frequency histogram of Murray cod (<i>Maccullochella peelii</i>) acceleration values measured during the study.</p

Location of the acoustic receiver array on the Wakool River and Yallakool Creek, south-eastern Australia, used to monitor locomotor activity of acoustically tagged Murray cod (<i>Maccullochella peelii</i>).

<p>Location of the acoustic receiver array on the Wakool River and Yallakool Creek, south-eastern Australia, used to monitor locomotor activity of acoustically tagged Murray cod (<i>Maccullochella peelii</i>).</p