Academic Panel: Can Self-Managed Systems be trusted?

Trust can be defined as to have confidence or faith in; a form of reliance or certainty based on past experience; to allow without fear; believe; hope: expect and wish; and extend credit to. The issue of trust in computing has always been a hot topic, especially notable with the proliferation of services over the Internet, which has brought the issue of trust and security right into the ordinary home. Autonomic computing brings its own complexity to this. With systems that self-manage, the internal decision making process is less transparent and the ‘intelligence’ possibly evolving and becoming less tractable. Such systems may be used from anything from environment monitoring to looking after Granny in the home and thus the issue of trust is imperative. To this end, we have organised this panel to examine some of the key aspects of trust. The first section discusses the issues of self-management when applied across organizational boundaries. The second section explores predictability in self-managed systems. The third part examines how trust is manifest in electronic service communities. The final discussion demonstrates how trust can be integrated into an autonomic system as the core intelligence with which to base adaptivity choices upon

Evolution of Process Choreographies in DYCHOR

Process-aware information systems have to be frequently adapted due to business process changes. One important challenge not adequately addressed so far concerns the evolution of process choreographies, i.e., the change of interactions between partner processes in a cross-organizational setting. If respective modifications are applied in an uncontrolled manner, inconsistencies or errors might occur in the sequel. In particular, modifications of private processes performed by a single party may affect the implementation of the private processes of partners as well. In this paper we present the DYCHOR (DYnamic CHOReographies) framework which allows process engineers to detect how changes of private processes may affect related public views and - if so - how they can be propagated to the public and private processes of partners. In particular, DYCHOR exploits the semantics of the applied changes in order to automatically determine the adaptations necessary for the partner processes. Altogether our framework provides an important contribution towards the realization of adaptive, cross-organizational processes

Radioactive Phosphorylation of Alcohols to Monitor Biocatalytic Diels-Alder Reactions

Nature has efficiently adopted phosphorylation for numerous biological key processes, spanning from cell signaling to energy storage and transmission. For the bioorganic chemist the number of possible ways to attach a single phosphate for radioactive labeling is surprisingly small. Here we describe a very simple and fast one-pot synthesis to phosphorylate an alcohol with phosphoric acid using trichloroacetonitrile as activating agent. Using this procedure, we efficiently attached the radioactive phosphorus isotope 32P to an anthracene diene, which is a substrate for the Diels-Alderase ribozyme—an RNA sequence that catalyzes the eponymous reaction. We used the 32P-substrate for the measurement of RNA-catalyzed reaction kinetics of several dye-labeled ribozyme variants for which precise optical activity determination (UV/vis, fluorescence) failed due to interference of the attached dyes. The reaction kinetics were analyzed by thin-layer chromatographic separation of the 32P-labeled reaction components and densitometric analysis of the substrate and product radioactivities, thereby allowing iterative optimization of the dye positions for future single-molecule studies. The phosphorylation strategy with trichloroacetonitrile may be applicable for labeling numerous other compounds that contain alcoholic hydroxyl groups

Super-Resolution Dynamic Imaging of Dendritic Spines Using a Low-Affinity Photoconvertible Actin Probe

The actin cytoskeleton of dendritic spines plays a key role in morphological aspects of synaptic plasticity. The detailed analysis of the spine structure and dynamics in live neurons, however, has been hampered by the diffraction-limited resolution of conventional fluorescence microscopy. The advent of nanoscopic imaging techniques thus holds great promise for the study of these processes. We implemented a strategy for the visualization of morphological changes of dendritic spines over tens of minutes at a lateral resolution of 25 to 65 nm. We have generated a low-affinity photoconvertible probe, capable of reversibly binding to actin and thus allowing long-term photoactivated localization microscopy of the spine cytoskeleton. Using this approach, we resolve structural parameters of spines and record their long-term dynamics at a temporal resolution below one minute. Furthermore, we have determined changes in the spine morphology in response to pharmacologically induced synaptic activity and quantified the actin redistribution underlying these changes. By combining PALM imaging with quantum dot tracking, we could also simultaneously visualize the cytoskeleton and the spine membrane, allowing us to record complementary information on the morphological changes of the spines at super-resolution

A review of source tracking techniques for fine sediment within a catchment

Excessive transport of fine sediment, and its associated pollutants, can cause detrimental impacts in aquatic environments. It is therefore important to perform accurate sediment source apportionment to identify hot spots of soil erosion. Various tracers have been adopted, often in combination, to identify sediment source type and its spatial origin; these include fallout radionuclides, geochemical tracers, mineral magnetic properties and bulk and compound-specific stable isotopes. In this review, the applicability of these techniques to particular settings and their advantages and limitations are reviewed. By synthesizing existing approaches, that make use of multiple tracers in combination with measured changes of channel geomorphological attributes, an integrated analysis of tracer profiles in deposited sediments in lakes and reservoirs can be made. Through a multi-scale approach for fine sediment tracking, temporal changes in soil erosion and sediment load can be reconstructed and the consequences of changing catchment practices evaluated. We recommend that long-term, as well as short-term, monitoring of riverine fine sediment and corresponding surface and subsurface sources at nested sites within a catchment are essential. Such monitoring will inform the development and validation of models for predicting dynamics of fine sediment transport as a function of hydro-climatic and geomorphological controls. We highlight that the need for monitoring is particularly important for hilly catchments with complex and changing land use. We recommend that research should be prioritized for sloping farmland-dominated catchments

A review of source tracking techniques for fine sediment within a catchment

Excessive transport of fine sediment, and its associated pollutants, can cause detrimental impacts in aquatic environments. It is therefore important to perform accurate sediment source apportionment to identify hot spots of soil erosion. Various tracers have been adopted, often in combination, to identify sediment source type and its spatial origin; these include fallout radionuclides, geochemical tracers, mineral magnetic properties and bulk and compound-specific stable isotopes. In this review, the applicability of these techniques to particular settings and their advantages and limitations are reviewed. By synthesizing existing approaches, that make use of multiple tracers in combination with measured changes of channel geomorphological attributes, an integrated analysis of tracer profiles in deposited sediments in lakes and reservoirs can be made. Through a multi-scale approach for fine sediment tracking, temporal changes in soil erosion and sediment load can be reconstructed and the consequences of changing catchment practices evaluated. We recommend that long-term, as well as short-term, monitoring of riverine fine sediment and corresponding surface and subsurface sources at nested sites within a catchment are essential. Such monitoring will inform the development and validation of models for predicting dynamics of fine sediment transport as a function of hydro-climatic and geomorphological controls. We highlight that the need for monitoring is particularly important for hilly catchments with complex and changing land use. We recommend that research should be prioritized for sloping farmland-dominated catchments

The stable isotope geochemistry and cosmochemistry of cadmium

Massenabhängige Variationen der Cd Isotopenzusammensetzung in Gesteinen und Steinmeteoriten wurden mit MC-ICPMS (multiple collector inductively coupled plasma mass spectrometry) präzise und akkurat bestimmt. Aus den Daten ergibt sich, dass anorganische geologische Prozesse, im Gegensatz zu Verdampfungs- und Kondensationsprozessen, nicht zu grossen Cd Isotopenfraktionierungen führen. Messungen an primitiven und differenzierten Steinmeteoriten ermöglichten daher neue Einblicke in Verdampfungs- und Kondensationsprozesse bei der Entstehung des Sonnensystems. Anhand von Cd Verdampfungsexperimenten wird gezeigt, dass präzise Messungen von Massenabhängigkeiten Aussagen über Fraktionierungsmechanismen erlauben. Durch die Auswertung von Nd Isotopendaten zeigte sich, dass die instrumentelle Massendiskriminierung mit dem ?generalized power law? präzise beschrieben werden kann

A Simple Competitive Protein-Binding Assay for Cyclic Adenosine 3´:5´-monophosphate

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