Ocorrência da mosca dos favos Pseudohpocera kerteszi (Diptera: Phoridae) em colméias de abelhas melíferas africanizadas no Rio Grande do Sul.

bitstream/item/31359/1/comunicado-178.pdfMeta: 2008

Tubulin subunit carboxyl termini determine polymerization efficiency

Cleavage of tubulin by subtilisin removes a small (Mr < 2000) fragment from the C-terminal end of both α and β subunits. The resulting protein is much reduced in negative charge. The cleaved, less acidic protein retains its competence to polymerize in a GTP-dependent and cold-, GDP-, and podophyllotoxin-sensitive manner and assembles into sheets or bundles of twisted filaments. The critical concentration for polymerization of the cleaved protein is about 50-fold lower than that for intact tubulin. It is proposed that the C termini of the subunits normally impede polymerization

Tubulin, hybrid dimers, and tubulin S. Stepwise charge reduction and polymerization

Limited proteolysis of rat brain tubulin (αβ) by subtilisin cleaves a 1-2-kDa fragment from the carboxyl-terminal ends of both the α and β subunits with a corresponding loss in negative charge of the proteins. The β subunit is split much more rapidly (and exclusively at 5 ° C), yielding a protein with cleaved β and intact α subunit, called α βs, which is of intermediate charge. Further proteolysis cleaves the carboxyl terminus of the α subunit leading, irreversibly, to the doubly cleaved product, named tubulin S, with a composition α s β s. Both cleavage products are polymerization-competent and their polymers are resistant to 1 mM Ca2+- and 0.24 M NaCl-induced depolymerization. The two polymers differ in that the αβs polymer is stable to cold, GDP, and podophyllotoxin, whereas tubulin S polymer is disassembled by these agents; moreover, αβs forms ring-shaped polymers, whereas αsβs forms filaments associated into bundles and sheets. Tubulin S co-polymerizes with native tubulin yielding a mixed product of intermediate stability. The presence of low mole fractions of tubulin S leads to a marked reduction in the critical concentration for polymerization of the mixture

New Ultraviolet Extinction Curves for Interstellar Dust in M31

New low-resolution UV spectra of a sample of reddened OB stars in M31 were obtained with HST/STIS to study the wavelength dependence of interstellar extinction and the nature of the underlying dust grain populations. Extinction curves were constructed for four reddened sightlines in M31 paired with closely matching stellar atmosphere models. The new curves have a much higher S/N than previous studies. Direct measurements of N(H I) were made using the Ly$\alpha$ absorption lines enabling gas-to-dust ratios to be calculated. The sightlines have a range in galactocentric distance of 5 to 14 kpc and represent dust from regions of different metallicities and gas-to-dust ratios. The metallicities sampled range from Solar to 1.5 Solar. The measured curves show similarity to those seen in the Milky Way and the Large Magellanic Cloud. The Maximum Entropy Method was used to investigate the dust composition and size distribution for the sightlines observed in this program finding that the extinction curves can be produced with the available carbon and silicon abundances if the metallicity is super-Solar.Comment: ApJ, in press, 9 pages, 5 figure

Applied Force and sEMG Muscle Activity Required To Operate Pistol Grip Control in an Electric Utility Aerial Bucket

Electric utility line workers report high levels of fatigue in forearm muscles when operating a conventional pistol grip control in aerial buckets. This study measured the applied force and surface electromyographic (sEMG) signals from four upper extremity muscles required to operate the pistol grip control in two tasks. The first task was movement of the pistol grip in six directions (up/down, forward/rearward, clockwise/counter-clockwise), and the second task was movement of the bucket from its resting position on the truck bed to an overhead conductor on top of a 40 ft tall pole. The force applied to the pistol grip was measured in 14 aerial bucket trucks, and sEMG activity was measured on eight apprentice line workers. The applied force required to move the pistol grip control in the six directions ranged from 12 to 15 lb. The sEMG activity in the extensor digitorum communis (EDC) forearm muscle was approximately twice as great or more than the other three muscles (flexor digitorum superficialis, triceps, and biceps). Line workers exerted 14 to 30% MVCEMG to move the pistol grip in the six directions. Average %MVCEMG of the EDC to move the bucket from the truck platform to an overhead line ranged from 26 to 30% across the four phases of the task. The sEMG findings from this study provide physiologic evidence to support the anecdotal reports of muscle fatigue from line workers after using the pistol grip control for repeated, long durations

Can we predict the duration of an interglacial?

Differences in the duration of interglacials have long been apparent in palaeoclimate records of the Late and Middle Pleistocene. However, a systematic evaluation of such differences has been hampered by the lack of a metric that can be applied consistently through time and by difficulties in separating the local from the global component in various proxies. This, in turn, means that a theoretical framework with predictive power for interglacial duration has remained elusive. Here we propose that the interval between the terminal oscillation of the bipolar seesaw and three thousand years (kyr) before its first major reactivation provides an estimate that approximates the length of the sea-level highstand, a measure of interglacial duration. We apply this concept to interglacials of the last 800 kyr by using a recently-constructed record of interhemispheric variability. The onset of interglacials occurs within 2 kyr of the boreal summer insolation maximum/precession minimum and is consistent with the canonical view of Milankovitch forcing pacing the broad timing of interglacials. Glacial inception always takes place when obliquity is decreasing and never after the obliquity minimum. The phasing of precession and obliquity appears to influence the persistence of interglacial conditions over one or two insolation peaks, leading to shorter (~ 13 kyr) and longer (~ 28 kyr) interglacials. Glacial inception occurs approximately 10 kyr after peak interglacial conditions in temperature and CO2, representing a characteristic timescale of interglacial decline. Second-order differences in duration may be a function of stochasticity in the climate system, or small variations in background climate state and the magnitude of feedbacks and mechanisms contributing to glacial inception, and as such, difficult to predict. On the other hand, the broad duration of an interglacial may be determined by the phasing of astronomical parameters and the history of insolation, rather than the instantaneous forcing strength at inception

Monomer dynamics of a wormlike chain

We derive the stochastic equations of motion for a tracer that is tightly attached to a semiflexible polymer and confined or agitated by an externally controlled potential. The generalised Langevin equation, the power spectrum, and the mean-square displacement for the tracer dynamics are explicitly constructed from the microscopic equations of motion for a weakly bending wormlike chain by a systematic coarse-graining procedure. Our accurate analytical expressions should provide a convenient starting point for further theoretical developments and for the analysis of various single-molecule experiments and of protein shape fluctuations.Comment: 6 pages, 4 figure

The Ever Changing Circumstellar Nebula Around UW Centauri

We present new images of the reflection nebula surrounding the R Coronae Borealis Star, UW Cen. This nebula, first detected in 1990, has changed its appearance significantly. At the estimated distance of UW Cen, this nebula is approximately 0.6 ly in radius so the nebula cannot have physically altered in only 8 years. Instead, the morphology of the nebula appears to change as different parts are illuminated by light from the central star modulated by shifting thick dust clouds near its surface. These dust clouds form and dissipate at irregular intervals causing the well-known declines in the R Coronae Borealis (RCB) stars. In this way, the central star acts like a lighthouse shining through holes in the dust clouds and lighting up different portions of the nebula. The existence of this nebula provides clues to the evolutionary history of RCB stars possibly linking them to the Planetary Nebulae and the final helium shell flash stars.Comment: To be published in ApJ Letters. 5 pages, 3 figures (2 in color