1,441 research outputs found
Spectral Formation in X-Ray Pulsar Accretion Columns
We present the first self-consistent model for the dynamics and the radiative
transfer occurring in bright X-ray pulsar accretion columns, with a special
focus on the role of the shock in energizing the emerging X-rays. The pressure
inside the accretion column of a luminous X-ray pulsar is dominated by the
photons, and consequently the equations describing the coupled
radiative-dynamical structure must be solved simultaneously. Spectral formation
in these sources is therefore a complex, nonlinear phenomenon. We obtain the
analytical solution for the Green's function describing the upscattering of
monochromatic radiation injected into the column from the thermal mound located
near the base of the flow. The Green's function is convolved with a Planck
distribution to model the X-ray spectrum resulting from the reprocessing of
blackbody photons produced in the thermal mound. These photons diffuse through
the infalling gas and eventually escape out the walls of the column, forming
the observed X-ray spectrum. We show that the resulting column-integrated,
phase-averaged spectrum has a power-law shape at high energies and a blackbody
shape at low energies, in agreement with the observational data for many X-ray
pulsars.Comment: Accepted for publication in ApJ Letters. Several typos noticed during
the proof review were correcte
Analytical solution of the Gross-Neveu model at finite density
Recent numerical calculations have shown that the ground state of the
Gross-Neveu model at finite density is a crystal. Guided by these results, we
can now present the analytical solution to this problem in terms of elliptic
functions. The scalar potential is the superpotential of the non-relativistic
Lame Hamiltonian. This model can also serve as analytically solvable toy model
for a relativistic superconductor in the Larkin-Ovchinnikov-Fulde-Ferrell
phase.Comment: 5 pages, no figures, revtex; vs2: appendix with analytical proof of
self-consistency adde
Pseudo-affinity purification and formulation of a cell-culture derived whole influenza virus vaccine using magnetic sulfated cellulose particles
The production of viral vaccines usually employs different unit operations where formulation and filling are the final steps of downstream processing (DSP). However, complex DSP is often hard to realize in research laboratories focusing on novel vaccine candidates. Moreover, there are no real ready-to-use tools for high-throughput DSP of whole virus particles that can speed up development. Because of these needs we developed a new platform for easy and straightforward whole virus particle purification and formulation based on magnetic sulfated cellulose particles (MSCP)1,2.
Proof of concept was carried out with an influenza A/Puerto Rico/8/34 (H1N1) whole virus vaccine for the immunization of mice. The virus particles were produced in suspension MDCK cells, clarified, inactivated, and concentrated using a standard protocol. After diafiltration to low salt buffer, the virus particles were bound to the MSCP and the virus loaded MSCP were washed and resuspended in formulation buffer.
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Purification of cell culture-derived influenza virus via continuous chromatography
In vaccine production downstream processing often constitutes a bottleneck in terms of process productivity and economy. One way to design more efficient purification trains could be the implementation of continuous chromatographic methods.
The aim of this study was the purification of cell culture-derived influenza virus using continuous chromatography. Therefore, two chromatographic modes, flow through with CaptoCore (CC) beads and bind and elute with anion exchange (AEX) monoliths, were characterized for their ability to separate the virus from contaminating host cell protein and DNA. The starting material for the CC was treated with nuclease to decrease the DNA content and fragment size. Further, regeneration conditions for the chromatographic media, a prerequisite for successful continuous implementation, were identified and verified in sequential batch experiments.
Simulated moving bed chromatography (SMB) was performed in an open loop configuration using constant switching times. In case of the CC material, two columns were located in the separation zones and two additional columns were regenerated and equilibrated in detached zones. For the AEX runs, on the other hand, monoliths were used in a three zone configuration with detached high salt zone for regeneration.
Results in batch chromatography (BC) and SMB showed similar product yields in the range 60 to 100%. Contaminant depletion was \u3e98% DNA and \u3e58% protein for the AEX monoliths. Both the CC SMB and the BC resulted in comparable impurity levels (33.2 µg protein and 25.6 ng DNA per estimated 15 µg HA) but for BC a higher product yield (89% vs 72%) was achieved. In addition, the virus dilution during the flow through chromatography could be reduced in the cyclic steady state of the SMB by a factor of 1.8.
Overall, the separation performance of the BC has been successfully transferred to the continuous process
Gas-grain simulation experiment module conceptual design and gas-grain simulation facility breadboard development
This report delineates the Option portion of the Phase A Gas-Grain Simulation Facility study. The conceptual design of a Gas-Grain Simulation Experiment Module (GGSEM) for Space Shuttle Middeck is discussed. In addition, a laboratory breadboard was developed during this study to develop a key function for the GGSEM and the GGSF, specifically, a solid particle cloud generating device. The breadboard design and test results are discussed and recommendations for further studies are included. The GGSEM is intended to fly on board a low earth orbit (LEO), manned platform. It will be used to perform a subset of the experiments planned for the GGSF for Space Station Freedom, as it can partially accommodate a number of the science experiments. The outcome of the experiments performed will provide an increased understanding of the operational requirements for the GGSF. The GGSEM will also act as a platform to accomplish technology development and proof-of-principle experiments for GGSF hardware, and to verify concepts and designs of hardware for GGSF. The GGSEM will allow assembled subsystems to be tested to verify facility level operation. The technology development that can be accommodated by the GGSEM includes: GGSF sample generation techniques, GGSF on-line diagnostics techniques, sample collection techniques, performance of various types of sensors for environmental monitoring, and some off-line diagnostics. Advantages and disadvantages of several LEO platforms available for GGSEM applications are identified and discussed. Several of the anticipated GGSF experiments require the deagglomeration and dispensing of dry solid particles into an experiment chamber. During the GGSF Phase A study, various techniques and devices available for the solid particle aerosol generator were reviewed. As a result of this review, solid particle deagglomeration and dispensing were identified as key undeveloped technologies in the GGSF design. A laboratory breadboard version of a solid particle generation system was developed and characterization tests performed. The breadboard hardware emulates the functions of the GGSF solid particle cloud generator in a ground laboratory environment, but with some modifications, can be used on other platforms
Disk Diffusion Propagation Model for the Outburst of XTE J1118+480
We present a linear diffusion model for the evolution of the double-peaked
outburst in the transient source XTEJ1118+480. The model treats the two
outbursts as episodic mass deposition at the outer radius of the disk followed
by evolution of disk structure according to a diffusion process. We demonstrate
that light curves with fast-rise, exponential decay profile are a general
consequence of the diffusion process. Deconvolution of the light curve proves
to be feasible and gives an input function specifying mass deposition at the
outer disk edge as well as the total mass of the disk, both as functions of
time. The derived evolution of total disk mass can be correlated with the
observed evolution of the ~0.1 Hz QPO in the source reported in Wood et al.
(2000).Comment: 26 pages, 6 figures, accepted for publication in Ap
Northern Late Winter Planetary Waves: MRO/MARCI Observations and Mars Climate Model Simulations
As does Earth, Mars presents pronounced global atmospheric circulation patterns. Solar differential heating drives mean meridional overturning (Hadley) circulations which are deep and intense, are hemispherically asymmetric, and where a cross-equatorial single cell dominates. Within middle and high latitudes, thermally indirect eddy-driven (Ferrel) circulation cells have been indicated. Differently, however, large-amplitude orography on planetary and continental scales on Mars can force very non-Earth-like hemispheric circulation patterns. Recent observations from the Mars Reconnaissance Orbiter, "Mars Color Imager" (MARCI) instrument are utilized that emphasize water ice clouds in ultra-violet (UV) wavelengths, and these measurements have been binned into "daily global maps" (DGMs) of water-ice cloud optical depth. The presence of large-scale, extratropical quasi-stationary atmospheric wave disturbances in middle and late winter of the northern hemisphere have been found to be present in such DGMs. In combination with such observations, a full-physics Mars global climate model (NASA ARC marsgcm 2.1) is applied to place the observations into context. During late northern winter, it is found that strong, forced Rossby modes (i.e., planetary waves) exist, and with direct correlation to columnintegrated cloud opacity undulating spatial patterns. At this season, zonal wavenumber s = 2 dominates (in contrast to wavenumber s = 1), consistent with MGS/TES analyses at this particular season (Banfield et al., 2003). Large-scale, planetary waves dictate the "coherence" of the northern polar vortex. Fundamentally, such forced planetary waves influence the polar vortex's impermeability (wave-induced) to tracer transport (e.g., dust and water-ice aerosol) and temporal mean water vapor spatial variations. The large-scale dynamical features of such planetary waves will be highlighted and discussed
Kinematics of Multigrid Monte Carlo
We study the kinematics of multigrid Monte Carlo algorithms by means of
acceptance rates for nonlocal Metropolis update proposals. An approximation
formula for acceptance rates is derived. We present a comparison of different
coarse-to-fine interpolation schemes in free field theory, where the formula is
exact. The predictions of the approximation formula for several interacting
models are well confirmed by Monte Carlo simulations. The following rule is
found: For a critical model with fundamental Hamiltonian H(phi), absence of
critical slowing down can only be expected if the expansion of
in terms of the shift psi contains no relevant (mass) term. We also introduce a
multigrid update procedure for nonabelian lattice gauge theory and study the
acceptance rates for gauge group SU(2) in four dimensions.Comment: 28 pages, 8 ps-figures, DESY 92-09
A Quantitative Comparison of SMC, LMC, and Milky Way UV to NIR Extinction Curves
We present an exhaustive, quantitative comparison of all of the known
extinction curves in the Small and Large Magellanic Clouds (SMC and LMC) with
our understanding of the general behavior of Milky Way extinction curves. The
R_V dependent CCM relationship and the sample of extinction curves used to
derive this relationship is used to describe the general behavior of Milky Way
extinction curves. The ultraviolet portion of the SMC and LMC extinction curves
are derived from archival IUE data, except for one new SMC extinction curve
which was measured using HST/STIS observations. The optical extinction curves
are derived from new (for the SMC) and literature UBVRI photometry (for the
LMC). The near-infrared extinction curves are calculated mainly from 2MASS
photometry supplemented with DENIS and new JHK photometry. For each extinction
curve, we give R_V = A(V)/E(B-V) and N(HI) values which probe the same dust
column as the extinction curve. We compare the properties of the SMC and LMC
extinction curves with the CCM relationship three different ways: each curve by
itself, the behavior of extinction at different wavelengths with R_V, and
behavior of the extinction curve FM fit parameters with R_V. As has been found
previously, we find that a small number of LMC extinction curves are consistent
with the CCM relationship, but majority of the LMC and all of the SMC curves do
not follow the CCM relationship. For the first time, we find that the CCM
relationship seems to form a bound on the properties of all of the LMC and SMC
extinction curves. This result strengthens the picture of dust extinction
curves exhibit a continuum of properties between those found in the Milky Way
and the SMC Bar. (abridged)Comment: 18 pages, 10 figures, ApJ in pres
Oocyte maturity, oocyte fertilization and cleavage-stage embryo morphology are better in natural compared with high-dose gonadotrophin stimulated IVF cycles.
RESEARCH QUESTION
Does high-dose gonadotrophin stimulation have an effect on oocyte and early-stage embryo development?
DESIGN
This was a retrospective study including 616 natural cycle IVF (NC-IVF) and 167 conventional IVF (cIVF) cycles. In total, 2110 oocytes were retrieved and analysed in fresh cycles. In NC-IVF, only human chorionic gonadotrophin was applied to trigger ovulation. In cIVF, antagonist protocols with daily 150-300 IU of human menopausal gonadotrophins were performed. The effect of gonadotrophins on oocyte and early-stage embryo development was analysed. Primary outcomes were the occurrence of mature (metaphase II) oocytes, zygotes and embryos with good morphology at the cleavage stage 2 days after oocyte retrieval.
RESULTS
The mature oocyte rate (number of mature oocytes/number of retrieved oocytes) was higher in NC-IVF than cIVF cycles (89% versus 82%, adjusted odds ratio [aOR] 1.79, P = 0.001), as was the zygote rate per oocyte retrieved (70% versus 58%, aOR 1.76, P = 0.001) and the zygote rate per mature oocyte (79% versus 71%, aOR 1.62, P = 0.001). The percentage of zygotes that developed into cleavage-stage embryos was no different. For the transferred embryos, the probability of having a good embryo morphology with four blastomeres and a fragmentation of <10% (score 0) in cleavage-stage embryos was found to be higher in NC-IVF (proportional aOR for four blastomeres 2.00, P < 0.001; aOR 1.87 for a fragmentation score of 0, P = 0.003).
CONCLUSIONS
Oocyte maturity, oocyte fertilization and morphology of the cleavage-stage embryo are affected by high-dose gonadotrophin stimulation in fresh IVF cycles
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