5,240 research outputs found
Kondo effect of Co adatoms on Ag monolayers on noble metal surfaces
The Kondo temperature of single Co adatoms on monolayers of Ag on Cu
and Au(111) is determined using Scanning Tunneling Spectroscopy. of Co on
a single monolayer of Ag on either substrate is essentially the same as that of
Co on a homogenous Ag(111) crystal. This gives strong evidence that the
interaction of surface Kondo impurities with the substrate is very local in
nature. By comparing found for Co on Cu, Ag, and Au (111)-surfaces we
show that the energy scale of the many-electron Kondo state is insensitive to
the properties of surface states and to the energetic position of the projected
bulk band edges.Comment: 4 pages, 3 figure
Kondo temperature of magnetic impurities at surfaces
Based on the experimental observation, that only the close vicinity of a
magnetic impurity at metal surfaces determines its Kondo behaviour, we
introduce a simple model which explains the Kondo temperatures observed for
cobalt adatoms at the (111) and (100) surfaces of Cu, Ag, and Au. Excellent
agreement between the model and scanning tunneling spectroscopy (STS)
experiments is demonstrated. The Kondo temperature is shown to depend on the
occupation of the d-level determined by the hybridization between adatom and
substrate with a minimum around single occupancy.Comment: 4 pages, 2 figure
Robust nonparametric detection of objects in noisy images
We propose a novel statistical hypothesis testing method for detection of
objects in noisy images. The method uses results from percolation theory and
random graph theory. We present an algorithm that allows to detect objects of
unknown shapes in the presence of nonparametric noise of unknown level and of
unknown distribution. No boundary shape constraints are imposed on the object,
only a weak bulk condition for the object's interior is required. The algorithm
has linear complexity and exponential accuracy and is appropriate for real-time
systems. In this paper, we develop further the mathematical formalism of our
method and explore important connections to the mathematical theory of
percolation and statistical physics. We prove results on consistency and
algorithmic complexity of our testing procedure. In addition, we address not
only an asymptotic behavior of the method, but also a finite sample performance
of our test.Comment: This paper initially appeared in 2010 as EURANDOM Report 2010-049.
Link to the abstract at EURANDOM repository:
http://www.eurandom.tue.nl/reports/2010/049-abstract.pdf Link to the paper at
EURANDOM repository: http://www.eurandom.tue.nl/reports/2010/049-report.pd
A multimodal imaging workflow for monitoring CAR T cell therapy against solid tumor from whole-body to single-cell level
CAR T cell research in solid tumors often lacks spatiotemporal information and therefore, there is a need for a molecular tomography to facilitate high-throughput preclinical monitoring of CAR T cells. Furthermore, a gap exists between macro- and microlevel imaging data to better assess intratumor infiltration of therapeutic cells. We addressed this challenge by combining 3D µComputer tomography bioluminescence tomography (µCT/BLT), light-sheet fluorescence microscopy (LSFM) and cyclic immunofluorescence (IF) staining. Methods: NSG mice with subcutaneous AsPC1 xenograft tumors were treated with EGFR CAR T cell (± IL-2) or control BDCA-2 CAR T cell (± IL-2) (n = 7 each). Therapeutic T cells were genetically modified to co-express the CAR of interest and the luciferase CBR2opt. IL-2 was administered s.c. under the xenograft tumor on days 1, 3, 5 and 7 post-therapy-initiation at a dose of 25,000 IU/mouse. CAR T cell distribution was measured in 2D BLI and 3D µCT/BLT every 3-4 days. On day 6, 4 tumors were excised for cyclic IF where tumor sections were stained with a panel of 25 antibodies. On day 6 and 13, 8 tumors were excised from rhodamine lectin-preinjected mice, permeabilized, stained for CD3 and imaged by LSFM. Results: 3D µCT/BLT revealed that CAR T cells pharmacokinetics is affected by antigen recognition, where CAR T cell tumor accumulation based on target-dependent infiltration was significantly increased in comparison to target-independent infiltration, and spleen accumulation was delayed. LSFM supported these findings and revealed higher T cell accumulation in target-positive groups at day 6, which also infiltrated the tumor deeper. Interestingly, LSFM showed that most CAR T cells accumulate at the tumor periphery and around vessels. Surprisingly, LSFM and cyclic IF revealed that local IL-2 application resulted in early-phase increased proliferation, but long-term overstimulation of CAR T cells, which halted the early added therapeutic effect. Conclusion: Overall, we demonstrated that 3D µCT/BLT is a valuable non-isotope-based technology for whole-body cell therapy monitoring and investigating CAR T cell pharmacokinetics. We also presented combining LSFM and MICS for ex vivo 3D- and 2D-microscopy tissue analysis to assess intratumoral therapeutic cell distribution and status
Platinum Group Metal-Doped Tungsten Phosphates for Selective C-H Activation of Lower Alkanes
Platinum group metal (PGM)-based catalysts are known to be highly active in the total combustion of lower hydrocarbons. However, through an alternative catalyst design reported in this paper by isolating PGM-based active sites in a tungsten phosphate matrix, we present a class of catalysts for selective oxidation of n-butane, propane, and propylene that do not contain Mo or V as redox-active elements. Two different catalyst concepts have been pursued. Concept A: isolating Ru-based active sites in a tungsten phosphate matrix coming upon as ReO3-type structure. Concept B: dilution of PGM-based active sites through the synthesis of X-ray amorphous Ru tungsten phosphates supported on SiO2. Using a high-throughput screening approach, model catalysts over a wide compositional range were evaluated for C3 and C4 partial oxidation. Bulk crystalline and supported XRD amorphous phases with similar Ru/W/P compositions showed comparable performance. Hence, for these materials, composition is more crucial than the degree of crystallinity. Further studies for optimization on second-generation supported systems revealed even better results. High selectivity for n-butane oxidation to maleic anhydride and propane oxidation to an acrolein/acrylic acid has been achieved
The Apollo ATCA Platform
We have developed a novel and generic open-source platform - Apollo - which
simplifies the design of custom Advanced Telecommunications Computing
Architecture (ATCA) blades by factoring the design into generic infrastructure
and application-specific parts. The Apollo "Service Module" provides the
required ATCA Intelligent Platform Management Controller, power entry and
conditioning, a powerful system-on-module (SoM) computer, and flexible clock
and communications infrastructure. The Apollo "Command Module" is customized
for each application and typically includes two large field-programmable gate
arrays, several hundred optical fiber interfaces operating at speeds up to 28
Gbps, memories, and other supporting infrastructure. The command and service
module boards can be operated together or independently on the bench without
need for an ATCA shelf.Comment: Submitted to the Proceedings for TWEPP 201
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