Generalized gaugings and the field-antifield formalism

We discuss the algebra of general gauge theories that are described by the embedding tensor formalism. We compare the gauge transformations dependent and independent of an invariant action, and argue that the generic transformations lead to an infinitely reducible algebra. We connect the embedding tensor formalism to the field-antifield (or Batalin-Vilkovisky) formalism, which is the most general formulation known for general gauge theories and their quantization. The structure equations of the embedding tensor formalism are included in the master equation of the field-antifield formalism.Comment: 42 pages; v2: some clarifications and 1 reference added; version to be published in JHE

The human RAD54 recombinational DNA repair protein is a double-stranded DNA-dependent ATPase

DNA double-strand break repair through the RAD52 homologous recombination pathway in the yeast Saccharomyces cerevisiae requires, among others, the RAD51, RAD52, and RAD54 genes. The biological importance of homologous recombination is underscored by the conservation of the RAD52 pathway from fungi to humans. The critical roles of the RAD52 group proteins in the early steps of recombination, the search for DNA homology and strand exchange, are now becoming apparent. Here, we report the purification of the human Rad54 protein. We showed that human Rad54 has ATPase activity that is absolutely dependent on double-stranded DNA. Unexpectedly, the ATPase activity appeared not absolutely required for the DNA repair function of human Rad54 in vivo. Despite the presence of amino acid sequence motifs that are conserved in a large family of DNA helicases, no helicase activity of human Rad54 was observed on a variety of different DNA substrates. Possible functions of human Rad54 in homologous recombination that couple the energy gained from ATP hydrolysis to translocation along DNA, rather than disruption of base pairing, are discussed

Electric/magnetic duality for chiral gauge theories with anomaly cancellation

We show that 4D gauge theories with Green-Schwarz anomaly cancellation and possible generalized Chern-Simons terms admit a formulation that is manifestly covariant with respect to electric/magnetic duality transformations. This generalizes previous work on the symplectically covariant formulation of anomaly-free gauge theories as they typically occur in extended supergravity, and now also includes general theories with (pseudo-)anomalous gauge interactions as they may occur in global or local N=1 supersymmetry. This generalization is achieved by relaxing the linear constraint on the embedding tensor so as to allow for a symmetric 3-tensor related to electric and/or magnetic quantum anomalies in these theories. Apart from electric and magnetic gauge fields, the resulting Lagrangians also feature two-form fields and can accommodate various unusual duality frames as they often appear, e.g., in string compactifications with background fluxes.Comment: 37 pages; v2: typos corrected and 1 reference adde

Efficient cDNA cloning by direct phenotypic correction of a mutant human cell line (HPRT-) using an Epstein-Barr virus derived cDNA expression vector.

Human cells are, in general, poor recipients of foreign DNA, which has severely hampered the cloning of genes by direct phenotypic correction of deficient human cell lines after DNA mediated gene transfer. In this communication a methodology is presented which largely circumvents this problems. The method relies on the use of a recently developed episomal Epstein-Barr-virus-derived cDNA expression vector (Belt et al. (1989) Gene 84, 407-417). The cloning of hypoxanthine phosphoribosyltransferase (HPRT) cDNA, corresponding to a low abundant mRNA in wild type cells is used as a model system. Size fractionated poly (A)+ RNA from wild type cells, which resulted in an approximately 10 fold enrichment in HPRT mRNA, was used to construct a cDNA library of 25,000 independent clones in the pECV25 vector. An HPRT deficient human cell line was transfected and subsequently selected with hygromycin B for DNA uptake. In a small scale experiment only 7000 hygromycin BR transfectants were sufficient to isolate 2 independent HATR clones which were shown to replicate episomes harbouring HPRT cDNA. The first insert had a 5' untranslated region (UTR) and a 3' UTR perfectly in agreement with published data. The second cDNA clone harboured an unusually long 5' UTR and a shorter 3' UTR due to alternative polyadenylation of the HPRT transcript which has not been previously recognized

Symplectic structure of N=1 supergravity with anomalies and Chern-Simons terms

The general actions of matter-coupled N=1 supergravity have Peccei-Quinn terms that may violate gauge and supersymmetry invariance. In addition, N=1 supergravity with vector multiplets may also contain generalized Chern-Simons terms. These have often been neglected in the literature despite their importance for gauge and supersymmetry invariance. We clarify the interplay of Peccei-Quinn terms, generalized Chern-Simons terms and quantum anomalies in the context of N=1 supergravity and exhibit conditions that have to be satisfied for their mutual consistency. This extension of the previously known N=1 matter-coupled supergravity actions follows naturally from the embedding of the gauge group into the group of symplectic duality transformations. Our results regarding this extension provide the supersymmetric framework for studies of string compactifications with axionic shift symmetries, generalized Chern-Simons terms and quantum anomalies.Comment: 27 pages; v2: typos corrected; version to be published in Class.Quantum Gra

The LBV HR Car has a partner: Discovery of a companion with the VLTI

Luminous Blue Variables (LBVs) are massive stars caught in a post-main sequence phase, during which they are losing a significant amount of mass. As, on one hand, it is thought that the majority of massive stars are close binaries that will interact during their lifetime, and on the other, the most dramatic example of an LBV, Eta Car, is a binary, it would be useful to find other binary LBVs. We present here interferometric observations of the LBV HR Car done with the AMBER and PIONIER instruments attached to ESO's Very Large Telescope Interferometer (VLTI). Our observations, spanning two years, clearly reveal that HR Car is a binary star. It is not yet possible to constrain fully the orbit, and the orbital period may lie between a few years and several hundred years. We derive a radius for the primary in the system and possibly resolve as well the companion. The luminosity ratio in the H-band between the two components is changing with time, going from about 6 to 9. We also tentatively detect the presence of some background flux which remained at the 2% level until January 2016, but then increased to 6% in April 2016. Our AMBER results show that the emission line forming region of Br gamma is more extended than the continuum emitting region as seen by PIONIER and may indicate some wind-wind interaction. Most importantly, we constrain the total masses of both components, with the most likely range being 33.6 and 45 solar masses. Our results show that the LBV HR Car is possibly an Eta Car analog binary system with smaller masses, with variable components, and further monitoring of this object is definitively called for.Comment: A&A, in pres

Effective Actions near Singularities

We study the heterotic string compactified on K3 x T^2 near the line T=U, where the effective action becomes singular due to an SU(2) gauge symmetry enhancement. By `integrating in' the light W^\pm vector multiplets we derive a quantum corrected effective action which is manifestly SU(2) invariant and non-singular. This effective action is found to be consistent with a residual SL(2,Z) quantum symmetry on the line T=U. In an appropriate decompactification limit, we recover the known SU(2) invariant action in five dimensions.Comment: 33 pages, LaTeX. v2: cosmetic correction on titlepage. v3: references and note adde

Hypermultiplets and hypercomplex geometry from 6 to 3 dimensions

The formulation of hypermultiplets that has been developed for 5-dimensional matter multiplets is by dimensional reductions translated into the appropriate spinor language for 6 and 4 dimensions. We also treat the theories without actions that have the geometrical structure of hypercomplex geometry. The latter is the generalization of hyper-Kaehler geometry that does not require a Hermitian metric and hence corresponds to field equations without action. The translation tables of this paper allow the direct application of superconformal tensor calculus for the hypermultiplets using the available Weyl multiplets in 6 and 4 dimensions. Furthermore, the hypermultiplets in 3 dimensions that result from reduction of vector multiplets in 4 dimensions are considered, leading to a superconformal formulation of the c-map and an expression for the main geometric quantities of the hyper-Kaehler manifolds in the image of this map.Comment: 18 pages; v2: several clarifications in text and formulae, version to appear in Class.Quantum Gravit

Mouse model for the DNA repair/basal transcription disorder Trichothiodystrophy reveals cancer predisposition.

Patients with the nucleotide excision repair (NER) disorder xeroderma pigmentosum (XP) are highly predisposed to develop sunlight-induced skin cancer, in remarkable contrast to photosensitive NER-deficient trichothiodystrophy (TTD) patients carrying mutations in the same XPD gene. XPD encodes a helicase subunit of the dually functional DNA repair/basal transcription complex TFIIH. The pleiotropic disease phenotype is hypothesized to be, in part, derived from a repair defect causing UV sensitivity and, in part, from a subtle, viable basal transcription deficiency accounting for the cutaneous, developmental, and the typical brittle hair features of TTD. To understand the relationship between deficient NER and tumor susceptibility, we used a mouse model for TTD that mimics an XPD point mutation of a TTD patient in the mouse germline. Like the fibroblasts from the patient, mouse cells exhibit a partial NER defect, evident from the reduced UV-induced DNA repair synthesis (residual repair capacity approximately 25%), limited recovery of RNA synthesis after UV exposure, and a relatively mild hypersensitivity to cell killing by UV or 7,12-dimethylbenz[a]anthracene. In accordance with the cellular studies, TTD mice exhibit a modestly increased sensitivity to UV-induced inflammation and hyperplasia of the skin. In striking contrast to the human syndrome, TTD mice manifest a dear susceptibility to UV- and 7,12-dimethylbenz[a]anthracene-induced skin carcinogenesis, albeit not as pronounced as the totally NER-deficient XPA mice. These findings open up the possibility that TTD is associated with a so far unnoticed cancer predisposition and support the notion that a NER deficiency enhances cancer susceptibility. These findings have important implications for the etiology of the human disorder and for the impact of NER on carcinogenesis

The Matrix Theory S-Matrix

The technology required for eikonal scattering amplitude calculations in Matrix theory is developed. Using the entire supersymmetric completion of the v^4/r^7 Matrix theory potential we compute the graviton-graviton scattering amplitude and find agreement with eleven dimensional supergravity at tree level.Comment: 10 pages, RevTeX, no figure