Nebraska Rainbow Trout

Chapter 1 Lethal and Preferred Temperatures of Lake McConaughy Rainbow Trout Versus Domestic Strain Rainbow Trout By R. Vancil, G. Zuerlein and L. Hesse Chapter 2 A Nitrifying Filter-Cooling Condenser System for Total Water Re-use in Cold-Water Fish Holding or Rearing Applications By L. Hesse, G. Zuerlein and R. Vancil Chapter 3 Biochemical Genetic Analysis of Two Strains of Nebraska Rainbow Trout By J. Seeb and L. Wishard The self-sustaining rainbow trout population living in Lake Mcconaughy and the North Platte River tributaries is unique to the Great Plains region. The population spends most of its adult life in the reservoir, but moves into tributary streams to spawn. Two spawning runs occur. The largest is from September through November followed by a reduced migration. in March and April of the following spring. Rainbow trout fingerlings spend about a year in the stream before smolting and migrating to the reservoir. Fishing for Mcconaughy rainbows in the tributaries and reservoir began in the late 1940\u27s. Concern for the maintenance of this fishery prompted several investigations which were directed at development of a management plan. From these studies it was evident that the McConaughy rainbow possessed a selective advantage over hatchery origin fish stocked in the streams and reservoir. Temperatures, normally too high for successful rainbow survival, were often encountered in the streams and the eutrophying Lake McConaughy. This study was designed to compare the temperature tolerance and preference of Mcconaughy rainbow trout with hatchery origin fish. Chapter 1 describes the results of this study. Chapter 2 describes the laboratory facility developed to hold fish for relatively long periods while conducting the experiments. Chapter 3 contains the results of studies designed to characterize the genetic differences between hatchery fish from Massachusetts and the self-sustaining Mcconaughy rainbow trout. The Pacific Fisheries Research Laboratory in Olympia, Washington, was contracted to perform an electrophoresis characterization of the frequency of biochemical genetic variants among the McConaughy and hatchery rainbow groups

Gender and Coaching: Analysis of National Collegiate Athletic Association Division III Coaches' Discourse

The purpose of this qualitative research study was to gain insight into the language being used by Division III coaches. This study looked at patterns of discourse among Division III coaches, how those discourses were gendered, conscious choices that were being made by Division III coaches, and if Division III coaches perceived whether their coaching style aligned with their actual coaching discourse. Due to an increase in cross-gender coaching in collegiate athletics and a lack of research regarding the influence that gender has on coaching discourses, this research is pertinent to the future of college athletics. Six Division III coaches were interviewed using semi-structured interviews. Each coach then participated in two coaching session analyses in which their language was recorded during an authentic coaching session. Practice sessions, games, and scrimmages were considered authentic coaching sessions. Transcripts were produced from the audio recordings and discourse analysis was conducted. Using Gee’s (2005) seven building tasks supplemented by Connell’s (1985) Hegemonic Masculinity Theory, I identified several themes throughout Division III coaching discourse. I found that the Division III coaches in this study used their language to emphasize health, communication, and education. It was found that coaches embodied several identities, including motivator, disciplinarian, educator, and caregiver. Coaches also used language to initiate activities, to build relationships with their athletes, and to express their opinions. Although Division III coaches’ perceived communication approaches typically align with their actual coaching discourse, my findings show that problematic gendered language and hegemonic masculinity are prevalent in some Division III athletics programs

New Methods of Sample Preparation for Atom Probe Specimens

Magnetite is a common conductive mineral found on Earth and Mars. Disk-shaped precipitates approximately 40 nm in diameter have been shown to have manganese and aluminum concentrations. Atom-probe field-ion microscopy (APFIM) is the only technique that can potentially quantify the composition of these precipitates. APFIM will be used to characterize geological and planetary materials, analyze samples of interest for geomicrobiology; and, for the metrology of nanoscale instrumentation. Prior to APFIM sample preparation was conducted by electropolishing, the method of sharp shards (MSS), or Bosch process (deep reactive ion etching) with focused ion beam (FIB) milling as a final step. However, new methods are required for difficult samples. Many materials are not easily fabricated using electropolishing, MSS, or the Bosch process, FIB milling is slow and expensive, and wet chemistry and the reactive ion etching are typically limited to Si and other semiconductors. APFIM sample preparation using the dicing saw is commonly used to section semiconductor wafers into individual devices following manufacture. The dicing saw is a time-effective method for preparing high aspect ratio posts of poorly conducting materials. Femtosecond laser micromachining is also suitable for preparation of posts. FIB time required is reduced by about a factor of 10 and multi-tip specimens can easily be fabricated using the dicing saw

Ion–Hydrocarbon and/or Ion–Ion Interactions: Direct and Reverse Hofmeister Effects in a Synthetic Host

A combination of ¹H NMR spectroscopy, DLS, and turbidity measurements reveal that polarizable anions engender both the Hofmeister and reverse Hofmeister effects in positand <b>2</b>. Host <b>2</b> possesses two principal and distinctly different binding sites: a “soft” nonpolar pocket and a “hard” crown of ammonium cations. NMR spectroscopy reveals that anion affinity to both sites is comparable, with each site showing characteristic selectivities. NMR spectroscopy also reveals that anions competitively bind to the pocket and induce the Hofmeister effect in host–guest binding at very low concentrations (∼2 mM). Furthermore, the suite of techniques utilized demonstrates that anion binding to both sites leads to charge attenuation, aggregation, and finally precipitation (the reverse Hofmeister effect). Anion-induced precipitation generally correlated with affinity, and comparisons between the free host and its adamantane carboxylate (Ada-CO₂^–) complex reveals that the reverse Hofmeister effect is attenuated by blocking anion binding/charge attenuation at the nonpolar pocket