The Crowdsourced Replication Initiative: Investigating Immigration and Social Policy Preferences. Executive Report.

In an era of mass migration, social scientists, populist parties and social movements raise concerns over the future of immigration-destination societies. What impacts does this have on policy and social solidarity? Comparative cross-national research, relying mostly on secondary data, has findings in different directions. There is a threat of selective model reporting and lack of replicability. The heterogeneity of countries obscures attempts to clearly define data-generating models. P-hacking and HARKing lurk among standard research practices in this area.This project employs crowdsourcing to address these issues. It draws on replication, deliberation, meta-analysis and harnessing the power of many minds at once. The Crowdsourced Replication Initiative carries two main goals, (a) to better investigate the linkage between immigration and social policy preferences across countries, and (b) to develop crowdsourcing as a social science method. The Executive Report provides short reviews of the area of social policy preferences and immigration, and the methods and impetus behind crowdsourcing plus a description of the entire project. Three main areas of findings will appear in three papers, that are registered as PAPs or in process

Regulation of endoreduplication by protein phosphatase 2A in Arabidopsis thaliana leaves

Plants display an adaptive growth where the size of the plant can be influenced by the environment. For normal growth and development in Arabidopsis thaliana leaves the mitotic cell cycle is essential for cell proliferation and endoreduplication for cell expansion. Endoreduplication results in an increased number of sets of chromosomes in the nucleus, called polyploidy. Protein phosphatase 2A (PP2A) is a trimeric protein phosphatase, containing a structural subunit A, a regulatory subunit B and a catalytic C subunit. The PP2A-B’γ regulatory subunit is a negative regulator of plant defense and salicylic acid (SA) dependent transcriptional responses in the model plant A. thaliana. Salicylic acid is a key plant hormone in plant immunity against several of pathogens. A recent study suggested that SA signaling is needed to alter cell-cycle progression, and it is known to influence endoreduplication in Arabidopsis leaves. The regulation of endoreduplication and its physiological importance is still not well defined. In this study, flow cytometry analysis was used to determine the polyploidy levels of wild type, pp2a-b’ and mutants with altered SA levels. Immunoblot analysis was used to reveal if the changes of endoreduplication levels could be associated with differences in the abundance of the SA signaling protein NONEXPRESSER OF PATHOGENESIS RELATED GENES 1 (NPR1) between the mutants. My study revealed that endoreduplication is affected by photoperiod. Furthermore, analysis by flow cytometry revealed that pp2a-b’ mutants display enhanced polyploidy compared to wild type, indicating that PP2A-B’γ and its close homolog, PP2A–B’ζ, regulate endoreduplication. Immunoblot analysis of nuclear extracts revealed no connection between NPR1 protein abundance and endopolyploidy levels. The identification of PP2A as a regulator of endoreduplication reveals a previously unrecognized layer of regulation underlying the growth and productivity of plants

ACONITASE 3 is part of the ANAC017 transcription factor-dependent mitochondrial dysfunction response

Mitochondria are tightly embedded within metabolic and regulatory networks that optimize plant performance in response to environmental challenges. The best-known mitochondrial retrograde signaling pathway involves stress-induced activation of the transcription factor NAC DOMAIN CONTAINING PROTEIN 17 (ANAC017), which initiates protective responses to stress-induced mitochondrial dysfunction in Arabidopsis (Arabidopsis thaliana). Posttranslational control of the elicited responses, however, remains poorly understood. Previous studies linked protein phosphatase 2A subunit PP2A-B'gamma, a key negative regulator of stress responses, with reversible phosphorylation of ACONITASE 3 (ACO3). Here we report on ACO3 and its phosphorylation at Ser91 as key components of stress regulation that are induced by mitochondrial dysfunction. Targeted mass spectrometry-based proteomics revealed that the abundance and phosphorylation of ACO3 increased under stress, which required signaling through ANAC017. Phosphomimetic mutation at ACO3-Ser91 and accumulation of ACO3(S91D)-YFP promoted the expression of genes related to mitochondrial dysfunction. Furthermore, ACO3 contributed to plant tolerance against ultraviolet B (UV-B) or antimycin A-induced mitochondrial dysfunction. These findings demonstrate that ACO3 is both a target and mediator of mitochondrial dysfunction signaling, and critical for achieving stress tolerance in Arabidopsis leaves.Peer reviewe

Observing Many Researchers Using the Same Data and Hypothesis Reveals a Hidden Universe of Uncertainty

Breznau N, Rinke EM, Wuttke A, et al. Observing Many Researchers Using the Same Data and Hypothesis Reveals a Hidden Universe of Uncertainty. 2021.This study explores how researchers’ analytical choices affect the reliability of scientific findings. Most discussions of reliability problems in science focus on systematic biases. We broaden the lens to include conscious and unconscious decisions that researchers make during data analysis and that may lead to diverging results. We coordinated 161 researchers in 73 research teams and observed their research decisions as they used the same data to independently test the same prominent social science hypothesis: that greater immigration reduces support for social policies among the public. In this typical case of research based on secondary data, we find that research teams reported widely diverging numerical findings and substantive conclusions despite identical start conditions. Researchers’ expertise, prior beliefs, and expectations barely predicted the wide variation in research outcomes. More than 90% of the total variance in numerical results remained unexplained even after accounting for research decisions identified via qualitative coding of each team’s workflow. This reveals a universe of uncertainty that is hidden when considering a single study in isolation. The idiosyncratic nature of how researchers’ results and conclusions varied is a new explanation for why many scientific hypotheses remain contested. It calls for greater humility and clarity in reporting scientific findings

The Crowdsourced Replication Initiative: Investigating Immigration and Social Policy Preferences. Executive Report

Breznau N, Rinke EM, Wuttke A, et al. The Crowdsourced Replication Initiative: Investigating Immigration and Social Policy Preferences. Executive Report. 2019

How Many Replicators Does It Take to Achieve Reliability? Investigating Researcher Variability in a Crowdsourced Replication

The paper reports findings from a crowdsourced replication. Eighty-four replicator teams attempted to verify results reported in an original study by running the same models with the same data. The replication involved an experimental condition. A “transparent” group received the original study and code, and an “opaque” group received the same underlying study but with only a methods section and description of the regression coefficients without size or significance, and no code. The transparent group mostly verified the original study (95.5%), while the opaque group had less success (89.4%). Qualitative investigation of the replicators’ workflows reveals many causes of non-verification. Two categories of these causes are hypothesized, routine and non-routine. After correcting non-routine errors in the research process to ensure that the results reflect a level of quality that should be present in ‘real-world’ research, the rate of verification was 96.1 in the transparent group and 92.4 in the opaque group. Two conclusions follow: (1) Although high, the verification rate suggests that it would take a minimum of three replicators per study to achieve replication reliability of at least 95 confidence assuming ecological validity in this controlled setting, and (2) like any type of scientific research, replication is prone to errors that derive from routine and undeliberate actions in the research process. The latter suggests that idiosyncratic researcher variability might provide a key to understanding part of the “reliability crisis” in social and behavioral science and is a reminder of the importance of transparent and well documented workflows