Chemical vapor etching of GaAs by CH3I

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1994.Includes bibliographical references (leaves 174-179).by Charles Winslow Krueger.Ph.D

Teaching Data Science while Solving Real World Problems

The Data Science Team in EKU’s Noel Studio offers students the opportunity to improve and accomplish specialized research tasks. By being part of the team students have hands-on experience on how to collect, analyze, and report data to solve real-world problems.https://encompass.eku.edu/pedagogicon_postergallery/1002/thumbnail.jp

Differential expression of exosomal microRNAs in prefrontal cortices of schizophrenia and bipolar disorder patients

Exosomes are cellular secretory vesicles containing microRNAs (miRNAs). Once secreted, exosomes are able to attach to recipient cells and release miRNAs potentially modulating the function of the recipient cell. We hypothesized that exosomal miRNA expression in brains of patients diagnosed with schizophrenia (SZ) and bipolar disorder (BD) might differ from controls, reflecting either disease-specific or common aberrations in SZ and BD patients. The sources of the analyzed samples included McLean 66 Cohort Collection (Harvard Brain Tissue Resource Center), BrainNet Europe II (BNE, a consortium of 18 brain banks across Europe) and Boston Medical Center (BMC). Exosomal miRNAs from frozen postmortem prefrontal cortices with well-preserved RNA were isolated and submitted to profiling by Luminex FLEXMAP 3D microfluidic device. Multiple statistical analyses of microarray data suggested that certain exosomal miRNAs were differentially expressed in SZ and BD subjects in comparison to controls. RT-PCR validation confirmed that two miRNAs, miR-497 in SZ samples and miR-29c in BD samples, have significantly increased expression when compared to control samples. These results warrant future studies to evaluate the potential of exosome-derived miRNAs to serve as biomarkers of SZ and BD

Characterization of human immunodeficiency virus type 1 mutantswith decreased sensitivity to proteinase inhibitor Ro 31-8959

AbstractA human immunodeficiency virus type 1 (HIV-1) variant with highly reduced susceptibility to Ro 31-8959, an inhibitor of the viral proteinase, has been selected by repeated passage of wild-type virus in CEM cells in the presence of increasing concentrations of the inhibitor. Peptide sequences of the proteinase of selected virus were obtained from proviral DNA. Sequence comparison to wild-type (wt) proteinase demonstrated two amino acid substitutions in the resistant virus, a Gly to Val exchange at position 48 and a Leu to Met exchange at position 90. Furthermore, sequences of intermediate passage virus suggest contributions from positions 12, 36, 57, and 63 in early steps of resistance development. The selected virus showed a ca. 40-fold increase in 50% inhibitory concentration of Ro 31-8959. Growth kinetics of resistant virus were comparable to wild-type virus and the resistant genotype proved to be stable in the absence of inhibitor. Directed mutagenesis of the HIV-1 HXB2 proteinase at positions 48 and 90 suggested that each mutation alone led to a moderate decrease in sensitivity of the recombinant virus to proteinase inhibitor. However, a recombinant virus carrying both mutations in the proteinase gene showed a significant reduction in its sensitivity to Ro 31-8959 thus proving the importance of these exchanges for the resistance phenotype

Evolution of the Radial Size and Expansion of Coronal Mass Ejections Investigated by Combining Remote and In-Situ Observations

A fundamental property of coronal mass ejections (CMEs) is their radial expansion, which determines the increase in the CME radial size and the decrease in the CME magnetic field strength as the CME propagates. CME radial expansion can be investigated either by using remote observations or by in-situ measurements based on multiple spacecraft in radial conjunction. However, there have been only few case studies combining both remote and in-situ observations. It is therefore unknown if the radial expansion estimated remotely in the corona is consistent with that estimated locally in the heliosphere. To address this question, we first select 22 CME events between the years 2010 and 2013, which were well observed by coronagraphs and by two or three spacecraft in radial conjunction. We use the graduated cylindrical shell model to estimate the radial size, radial expansion speed, and a measure of the dimensionless expansion parameter of CMEs in the corona. The same parameters and two additional measures of the radial-size increase and magnetic-field-strength decrease with heliocentric distance of CMEs based on in-situ measurements are also calculated. For most of the events, the CME radial size estimated by remote observations is inconsistent with the in-situ estimates. We further statistically analyze the correlations of these expansion parameters estimated using remote and in-situ observations, and discuss the potential reasons for the inconsistencies and their implications for the CME space weather forecasting.Comment: Accepted by Ap

The Mineral Resources of Oregon

A mineral map is often of considerable value to those interested in miring work and development. Since there are very few, if any such maps for Oregon, we have endeavored to cover the ground, though only in an incomplete way, since the time we had for working upon it was limited and the data very meagre

Exosomal cell-to-cell transmission of alpha synuclein oligomers

Background: Aggregation of alpha-synuclein (αsyn) and resulting cytotoxicity is a hallmark of sporadic and familial Parkinson’s disease (PD) as well as dementia with Lewy bodies, with recent evidence implicating oligomeric and pre-fibrillar forms of αsyn as the pathogenic species. Recent in vitro studies support the idea of transcellular spread of extracellular, secreted αsyn across membranes. The aim of this study is to characterize the transcellular spread of αsyn oligomers and determine their extracellular location. Results: Using a novel protein fragment complementation assay where αsyn is fused to non-bioluminescent amino-or carboxy-terminus fragments of humanized Gaussia Luciferase we demonstrate here that αsyn oligomers can be found in at least two extracellular fractions: either associated with exosomes or free. Exosome-associated αsyn oligomers are more likely to be taken up by recipient cells and can induce more toxicity compared to free αsyn oligomers. Specifically, we determine that αsyn oligomers are present on both the outside as well as inside of exosomes. Notably, the pathway of secretion of αsyn oligomers is strongly influenced by autophagic activity. Conclusions: Our data suggest that αsyn may be secreted via different secretory pathways. We hypothesize that exosome-mediated release of αsyn oligomers is a mechanism whereby cells clear toxic αsyn oligomers when autophagic mechanisms fail to be sufficient. Preventing the early events in αsyn exosomal release and uptake by inducing autophagy may be a novel approach to halt disease spreading in PD and other synucleinopathies

Observations of Extreme ICME Ram Pressure Compressing Mercury's Dayside Magnetosphere to the Surface

Mercury's magnetosphere is known to be affected by enhanced ram pressures and magnetic fields inside interplanetary coronal mass ejections (ICMEs). Here we report detailed observations of an ICME compressing Mercury's dayside magnetosphere to the surface. A fast CME launched from the Sun on November 29 2013 impacted first MESSENGER, which was orbiting Mercury, on November 30 and later STEREO-A near 1 AU on December 1. Following the ICME impact, MESSENGER remained in the solar wind as the spacecraft traveled inwards and northwards towards Mercury's surface until it reached and passed its closest approach to the planet (at 371 km altitude) without crossing into the magnetosphere. The magnetospheric crossing finally occurred 1 minute before reaching the planet's nightside at 400 km altitude and 84$^\circ$N latitude, indicating the lack of dayside magnetosphere on this orbit. In addition, the peak magnetic field measured by MESSENGER at this time was 40% above the values measured in the orbits just prior to and after the ICME, a consequence of the magnetospheric compression. Using both a proxy method at Mercury and measurements at STEREO-A, we show that the extremely high ram pressure associated with this ICME was more than high enough to collapse Mercury's weak magnetosphere. As a consequence, the ICME plasma likely interacted with Mercury's surface, evidenced by enhanced sodium ions in the exosphere. The collapse of Mercury's dayside magnetosphere has important implications for the habitability of close-in exoplanets around M dwarf stars, as such events may significantly contribute to planetary atmospheric loss in these systems

Longitudinal conjunction between MESSENGER and STEREO A: Development of ICME complexity through stream interactions

We use data on an interplanetary coronal mass ejection (ICME) seen by MErcury Surface, Space ENvironment, GEochemistry, and Ranging (MESSENGER) and STEREO A starting on 29 December 2011 in a near‐perfect longitudinal conjunction (within 3°) to illustrate changes in its structure via interaction with the solar wind in less than 0.6 AU. From force‐free field modeling we infer that the orientation of the underlying flux rope has undergone a rotation of ∼80° in latitude and ∼65° in longitude. Based on both spacecraft measurements as well as ENLIL model simulations of the steady state solar wind, we find that interaction involving magnetic reconnection with corotating structures in the solar wind dramatically alters the ICME magnetic field. In particular, we observed a highly turbulent region with distinct properties within the flux rope at STEREO A, not observed at MESSENGER, which we attribute to interaction between the ICME and a heliospheric plasma sheet/current sheet during propagation. Our case study is a concrete example of a sequence of events that can increase the complexity of ICMEs with heliocentric distance even in the inner heliosphere. The results highlight the need for large‐scale statistical studies of ICME events observed in conjunction at different heliocentric distances to determine how frequently significant changes in flux rope orientation occur during propagation. These results also have significant implications for space weather forecasting and should serve as a caution on using very distant observations to predict the geoeffectiveness of large interplanetary transients.Key PointsICME complexity increases due to interaction with corotating structures in the solar windMagnetic reconnection between ICME and HPS/HCS alters the magnetic topology of the ICME flux ropeCaution on using distant observations to predict the geoeffectiveness of interplanetary transientsPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/134123/1/jgra52739.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/134123/2/jgra52739_am.pd