Para-infectious brain injury in COVID-19 persists at follow-up despite attenuated cytokine and autoantibody responses

To understand neurological complications of COVID-19 better both acutely and for recovery, we measured markers of brain injury, inflammatory mediators, and autoantibodies in 203 hospitalised participants; 111 with acute sera (1–11 days post-admission) and 92 convalescent sera (56 with COVID-19-associated neurological diagnoses). Here we show that compared to 60 uninfected controls, tTau, GFAP, NfL, and UCH-L1 are increased with COVID-19 infection at acute timepoints and NfL and GFAP are significantly higher in participants with neurological complications. Inflammatory mediators (IL-6, IL-12p40, HGF, M-CSF, CCL2, and IL-1RA) are associated with both altered consciousness and markers of brain injury. Autoantibodies are more common in COVID-19 than controls and some (including against MYL7, UCH-L1, and GRIN3B) are more frequent with altered consciousness. Additionally, convalescent participants with neurological complications show elevated GFAP and NfL, unrelated to attenuated systemic inflammatory mediators and to autoantibody responses. Overall, neurological complications of COVID-19 are associated with evidence of neuroglial injury in both acute and late disease and these correlate with dysregulated innate and adaptive immune responses acutely

Studies on the growth of Rhizocarpon geographicum in NW Scotland, and some implications for lichenometry

Scotland, a maritime subpolar environment (55–60°N), has seen relatively few applications of lichenometry – even though it offers much potential. Perhaps surprisingly, direct measurements of Rhizocarpon geographicum growth rates in Scotland are so far lacking. This study reports on the growth of this crustose areolate species from two sites in Assynt, NW Scotland, between 2002 and 2009. Repeat photography of 23 non-competing thalli growing under identical environmental conditions on a single vertical surface over 5 years at Inchnadamph showed growth rates to be a function of size – with larger thalli (10–30 mm) growing significantly faster than the smallest thalli (10 mm are 0.67 mm yr−1 (s.d. = 0.16). Studies on a second vertical surface near Lochinver, over 7 years, yielded complex growth data on a more mature population of R. geographicum thalli (10 mm) are slower (0.29 mm yr−1; s.d. = 0.12) than those at Inchnadamph. However, at this site, competition with other species rules out any meaningful comparison of growth rates between the two sites. Other growth processes were monitored over the five to seven-year study period, including hypothallus growth, areolae development, thallus coalescence, and inter-species competition – all have important implications for the use of Rhizocarpon species in lichenometry

Lichenometric dating: a commentary, in the light of some recent statistical studies

This commentary article discusses the relative merits of new mathematical approaches to lichenometry. It highlights their strong reliance on complex statistics; their user un-friendliness; and their occasional mistreatment of existing lichenometric techniques. The article proposes that the success of lichenometric dating over the past 50 years has stemmed from its relative simplicity, transparency, and general field applicability. It concludes that any new techniques which ignore these principles are likely to be unjustified, unsuitable to the user community and inappropriate for the subject matter. Furthermore, the article raises a more general philosophical question: can statistical complexity and high precision in a ‘geobotanical’ dating technique, fraught with high degrees of environmental variability and in-built uncertainty, ever be scientifically valid

Growth rates of Rhizocarpon geographicum: a review with new data from Iceland

This paper reviews evidence from previous growth-rate studies on lichens of the yellow-green species of Subgenus Rhizocarpon - the family most commonly used in lichenometric dating. New data are presented from Rhizocarpon section Rhizocarpon thalli growing on a moraine in southern Iceland over a period of 4.33 yr. Measurements of 38 lichen thalli, between 2001 and 2005, show that diametral growth rate (DGR, mm yr-1) is a function of thallus size. Growth rates increase rapidly in small thalli (50 mm diameter). Mean DGR in southern Iceland, between 2001 and 2005, was 0.64 mm yr-1 (SD = 0.24). The resultant growth-rate curve is parabolic and is best described by a third-order polynomial function. The striking similarity between these findings in Iceland and those of Armstrong ([1983]) in Wales implies that the shape of the growth-rate curve may be characteristic of Rhizocarpon geographicum lichens. The difference between the absolute growth rate in southern Iceland and Wales (ca. 66% faster) is probably a function of climate and micro-environment between the two sites. These findings have implications for previous lichenometric-dating studies, namely, that those studies which assume constant lichen growth rates over many decades are probably unreliable