Senior Recital: Miles Bonaker, trumpet

This recital is presented in partial fulfillment of requirements for the degree Bachelor of Music in Music Education. Mr. Bonaker studies trumpet with Douglas Lindsey.https://digitalcommons.kennesaw.edu/musicprograms/2147/thumbnail.jp

Species differences in the pattern of eicosanoids produced by inflamed and non-inflamed tissue

The synthesis of14C labelled arachidonic acid metabolites was measured in colonic tissues obtained from mice, rats, guinea pigs, rabbits, piglets and in colonic biopsies from humans during colonoscopy. The main eicosanoids formed after stimulation with calcium ionophore A23187 were: in humans, 15-hydroxy-eicosatetraenoic acid (15-HETE); in mice, 12-HETE; in rats, 12-HETE, 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT) and 6-keto-prostaglandine F1α (6kPGF1α); in guinea pigs, PGD2; in rabbits, 6kPGF1α, PGE2 and 15-HETE; and in pigs PGE2 and 12-HETE. In inflamed 15-HETE production was increased in man, HHT and 12-HETE production in rats and overall eicosanoid production in mice

Production of inflammatory mediators by human macrophages obtained from ascites

Ascites is a readily available source of human macrophages (Mø), which can be used to study Mø functions in vitro. We characterized the mediators of inflammation produced by human peritoneal Mø (hp-Mø) obtained from patients with portal hypertension and ascites. The production of the cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) was found to be lipopolysaccharide (LPS) concentration dependent (0–10 μg/ml) with a maximal production at 10 μg/ml and also dependent on the time of exposure to the stimulus (0–36 h). IL-1β, IL-6 and TNF-α production after LPS administration reached a plateau at 24 h. In vitro stimulation for 24 h with LPS does not influence the eicosanoid production from endogenous arachidonate. 13 min of exposure of the cells to the calcium ionophore A23187 gives a significant increase in eicosanoid production from both exogenous and endogenous arachidonate. The main eicosanoids produced are the 5-lipoxgenase products LTB4 and 5-hydroxyeicosatetraenoic acid (HETE). The increase in production of the other eicosanoids is not significant. The eicosanoid production depends on the stimulus concentration. The optimal A23187 concentration is 1 μM. Oxygen radical production was measured in the Mø by a flowcytometric method. The fluorescence intensity of phorbol 12-myristate 13-acetate stimulated and dihydro-rhodamine 123 loaded. hp-Mø increases significantly after 15 min. We conclude that LPS stimulation of hp-Mø from liver disease results in similar production of IL-1β, IL-6 and TNF-α, but that the profile of the eicosanoid production of these Mø stimulated with LPS and A23187 differs from Mø of other origin and species

New Spirometry Indices for Detecting Mild Airflow Obstruction.

The diagnosis of chronic obstructive pulmonary disease (COPD) relies on demonstration of airflow obstruction. Traditional spirometric indices miss a number of subjects with respiratory symptoms or structural lung disease on imaging. We hypothesized that utilizing all data points on the expiratory spirometry curves to assess their shape will improve detection of mild airflow obstruction and structural lung disease. We analyzed spirometry data of 8307 participants enrolled in the COPDGene study, and derived metrics of airflow obstruction based on the shape on the volume-time (Parameter D), and flow-volume curves (Transition Point and Transition Distance). We tested associations of these parameters with CT measures of lung disease, respiratory morbidity, and mortality using regression analyses. There were significant correlations between FEV1/FVC with Parameter D (r = -0.83; p < 0.001), Transition Point (r = 0.69; p < 0.001), and Transition Distance (r = 0.50; p < 0.001). All metrics had significant associations with emphysema, small airway disease, dyspnea, and respiratory-quality of life (p < 0.001). The highest quartile for Parameter D was independently associated with all-cause mortality (adjusted HR 3.22,95% CI 2.42-4.27; p < 0.001) but a substantial number of participants in the highest quartile were categorized as GOLD 0 and 1 by traditional criteria (1.8% and 33.7%). Parameter D identified an additional 9.5% of participants with mild or non-recognized disease as abnormal with greater burden of structural lung disease compared with controls. The data points on the flow-volume and volume-time curves can be used to derive indices of airflow obstruction that identify additional subjects with disease who are deemed to be normal by traditional criteria

Acción gen asociado con la expresión de heterosis en Jiló (Solanum gilo Raddi.)

The objectives of this work were to assess heterosis and its components in F 1 hybrids of a diallel cross among lines of scarlet eggplants (Solanum gilo Raddi, syn. S. aeth opicum L.), and to study the modes of gene action associated with its expression. Six lines (Morro Redondo, Branco Comprido, BGH-1544, Tinguá, Irajá, Comprido Verde Claro) and 15 hybrids resulting from a diallel cross among them (reciprocals excluded) were tested in a randomized complete block design with four replications. Traits under study were total yield, mean fruit mass and fruit length/diameter ratio. There was no evidence of epistatic gene action in the expression of any of the traits under study. Heterosis for yield could be explained by overdominance, but higher yields were only weakly associated with larger frequencies of dominant alleles an indication that yields may be markedly influenced by the action of relatively few loci with overdominant gene action. Heterosis for mean fruit mass was associated with incompletely dominant gene action, and larger fruit mass was associated with larger proportions of dominant alleles.Los objetivos de este trabajo fueron evaluar la heterosis y sus componentes en los híbridos F 1 de una dialélico entre líneas de jiló (Solanum gilo Raddi, syn. S. aethiopicum L.), y el estudio de los modos de acción de los genes asociados con su expresión. Seis líneas (Morro Redondo, Branco Comprido, BGH-1544, Tinguá, Irajá, Comprido Verde Claro) y 15 híbridos resultantes de un cruce dialélico entre ellos (recíprocos excluídos) se pusieron a prueba en un diseño de bloques completamente aleatorio con cuatro repeticiones. Los rasgos estudiados fueron producción total, peso promedio del fruto y la relación longitud/diámetro. No hubo evidencia de la acción de genes epistatic en la expresión de cualquiera de los rasgos en estudio. Para la producción total, la heterosis se explica por la acción de genes overdominance, ya que había una asociación débil, no significativa, entre los alelos dominantes y los valores de producción de frutas altas totales. La heterosis de masa media de la fruta se asoció con la acción del gen dominante incompleto, y la masa da fruta más grande se asoció con una mayor proporción de alelos dominantes. Para la relación longitud/diámetro (LD-1), la heterosis fue debida a la acción del gen dominante incompleto, y alelos dominantes se asociaron principalmente con menores valores LD-1.Fil: Matos Andrade, Thiago. Universidad Federal de Sergipe (Brasil)Fil: Lasmar, André. Universidad Federal de Lavras (Brasil)Fil: Maluf, Wilson Roberto. Universidad Federal de Lavras (Brasil)Fil: Gomes, Luiz Antonio Augusto. Universidad Federal de Lavras (Brasil)Fil: Gonçalvez, Ranoel José Souza de. Universidad Federal de Campina Grande (Brasil)Fil: Fitzgerald Blank, Arie. Universidad Federal de Sergipe (Brasil

Levels of soluble intercellular adhesion molecule 1, eicosanoids and cytokines in ascites of patients with liver cirrhosis, peritoneal cancer and spontaneous bacterial peritonitis

The levels of the eicosanoids leukotriene B4, prostaglandin E2, prostacycline and thromboxane B2, the cytokines interleukin-1β, interleukin-6 and tumour necrosis factor-α and soluble intercellular adhesion molecule 1 were measured in ascites and plasma samples of patients with liver cirrhosis (53), peritoneal cancer (26) and spontaneous bacterial peritonitis (10) to assess their value as a possible diagnostic and prognostic parameter in the course of the disease. Soluble intercellular adhesion molecule 1, of the eicosanoids prostaglandin E2 and leukotriene B4, and the protein concentration in ascites were all significantly elevated in ascites of patients with peritoneal cancer in comparison to ascites of patients with liver cirrhosis. In ascites of patients with spontaneous bacterial infection interleukin-6 concentration was significantly elevated and the protein concentration was significantly lower in comparison to the other two groups. None of these parameters, however, seems to be of practical use as a diagnostic parameter, as there is an overlap between all the levels of these mediators in ascites of liver cirrhosis, peritoneal cancer and spontaneous bacterial peritonitis group. Soluble intercellular adhesion molecule 1 levels were much higher in plasma than in ascites, in contrast to interleukin-6 levels which were much higher in ascites than in plasma. Soluble intercellular adhesion molecule 1 in ascites correlated with soluble intercellular adhesion molecule 1 in plasma (r = 0.6926, P = 0.0001). Soluble intercellular adhesion molecule 1, interleukin-6 and the number of polymorphonuclear cells in peritoneal fluid correlated during episodes of infection in patients with a peritonitis. For this reason soluble intercellular adhesion molecule 1 and interleukin-6 could be of prognostic value for patients with peritonitis