A search for identity in the narrative maelstrom: a psychoanalytic approach to Ishmael in Moby-Dick

Thesis (M.A.) University of Alaska Fairbanks, 2002'Call me Ishmael.' This opening line has confronted many a wary student first opening Moby-Dick. This thesis also confronts this line, by way of the enigma that is the narrator of the novel. Critics have long noted the fragmented nature of Moby-Dick, especially its oddly varying points of view. The book opens with a homodiegetic narrator telling a sea adventure tale, but by the end is dominated by a heterodiegetic narrator telling the story of Ahab's tragedy. Using classic Freudian psychology and some Lacanian theory, this thesis makes a case for the complexity and importance of Ishmael in the structure and theme of the novel. Dividing the book into separate narratives representing Ishmael's ego, super-ego, and id, this thesis argues that Ishmael develops from a naive, green sailor into an experienced whaleman with a healthier coherent personality. It is in the telling of the story that he is finally able to manifest this healthier personality.General introduction -- Ishmael and the ego: his hand against every man -- Ishmael and the super-ego: an apology for the encyclopedic narrative -- Ishmael, Ahab and the Id part one: the Id's power over Ishmael -- Ishmael, Ahab and the Id part two: desire, seduction and awareness -- Conclusion

Comparison of two methods for describing the strain profiles in quantum dots

The electronic structure of interfaces between lattice-mismatched semiconductor is sensitive to the strain. We compare two approaches for calculating such inhomogeneous strain -- continuum elasticity (CE, treated as a finite difference problem) and atomistic elasticity (AE). While for small strain the two methods must agree, for the large strains that exist between lattice-mismatched III-V semiconductors (e.g. 7% for InAs/GaAs outside the linearity regime of CE) there are discrepancies. We compare the strain profile obtained by both approaches (including the approximation of the correct C_2 symmetry by the C_4 symmetry in the CE method), when applied to C_2-symmetric InAs pyramidal dots capped by GaAs.Comment: To appear in J. Appl. Physic

Cold shock induction of recombinant Arctic environmental genes

Published Version, also available at http://dx.doi.org/10.1186/s12896-015-0185-1Background: Heterologous expression of psychrophilic enzymes in E. coli is particularly challenging due to their intrinsic instability. The low stability is regarded as a consequence of adaptation that allow them to function at low temperatures. Recombinant production presents a significant barrier to their exploitation for commercial applications in industry. Methods: As part of an enzyme discovery project we have investigated the utility of a cold-shock inducible promoter for low-temperature expression of five diverse genes derived from the metagenomes of marine Arctic sediments. After evaluation of their production, we further optimized for soluble production by building a vector suite from which the environmental genes could be expressed as fusions with solubility tags. Results: We found that the low-temperature optimized system produced high expression levels for all putatively cold-active proteins, as well as reducing host toxicity for several candidates. As a proof of concept, activity assays with one of the candidates, a putative chitinase, showed that functional protein was obtained using the lowtemperature optimized vector suite. Conclusions: We conclude that a cold-shock inducible system is advantageous for the heterologous expression of psychrophilic proteins, and may also be useful for expression of toxic mesophilic and thermophilic proteins where properties of the proteins are deleterious to the host cell growth

Reduced-order modelling of vortex-induced vibration of catenary riser

A new reduced-order model capable of analyzing the vortex-induced vibration of catenary riser in the ocean current has been developed. This semi analytical-numerical approach is versatile and allows for a significant reduction in computational effort for the analysis of fluid-riser interactions. The incoming current flow is assumed to be steady, uniform, unidirectional and perpendicular to the riser plane of initial equilibrium curvatures. The equations of riser 3-D motion are based on a pinned-pinned, tensioned-beam or flexural cable, modelling which accounts for overall effects of riser bending, extensibility, sag, inclination and structural nonlinearities. The unsteady hydrodynamic forces associated with cross-flow and in-line vibrations are modelled as distributed van der Pol wake oscillators. This hydrodynamic model has been modified in order to capture the effect of varying initial curvatures of the inclined flexible cylinder and to describe the space-time fluctuation of lift and drag forces. Depending on the vortex-excited in-plane/out-of-plane modes and system fluid-structure parameters, the parametric studies are carried out to determine the maximum response amplitudes of catenary risers, along with the occurrence of uni-modal lock-in phenomenon. The obtained results highlight the effect of initial curvatures and geometric nonlinearities on the nonlinear dynamics of riser undergoing vortex-induced vibration

Decision region approximation by polynomials or neural networks

We give degree of approximation results for decision regions which are defined by polynomial and neural network parametrizations. The volume of the misclassified region is used to measure the approximation error, and results for the degree of L1 approximation of functions are used. For polynomial parametrizations, we show that the degree of approximation is at least 1, whereas for neural network parametrizations we prove the slightly weaker result that the degree of approximation is at least r, where r can be any number in the open interval (0, 1)

An Interval-Censored Proportional Hazards Model

We fit a Cox proportional hazards (PH) model to interval-censored survival data by first subdividing each individual\u27s failure interval into non-overlapping sub-intervals. Using the set of all interval endpoints in the data set, those that fall into the individual\u27s interval are then used as the cut points for the sub-intervals. Each sub-interval has an accompanying weight calculated from a parametric Weibull model based on the current parameter estimates. A weighted PH model is then fit with multiple lines of observations corresponding to the sub-intervals for each individual, where the lower end of each sub-interval is used as the observed failure time with the accompanying weights incorporated. Right-censored observations are handled in the usual manner. We iterate between estimating the baseline Weibull distribution and fitting the weighted PH model until the regression parameters of interest converge. The regression parameter estimates are fixed as an offset when we update the estimates of the Weibull distribution and recalculate the weights. Our approach is similar to Satten et al.\u27s (1998) method for interval-censored survival analysis that used imputed failure times generated from a parametric model in a PH model. Simulation results demonstrate apparently unbiased parameter estimation for the correctly specified Weibull model and little to no bias for a mis-specified log-logistic model. Breast cosmetic deterioration data and ICU hyperlactemia data are analyzed

Engineering low-temperature expression systems for heterologous production of cold-adapted enzymes

Source at https://doi.org/10.1080/21655979.2015.1128589.Production of psychrophilic enzymes in the commonly used mesophilic expression systems is hampered by low intrinsic stability of the recombinant enzymes at the optimal host growth temperatures. Unless strategies for low-temperature expression are advanced, research on psychrophilic enzymes may end up being biased toward those that can be stably produced in commonly used mesophilic host systems. Two main strategies are currently being explored for the development of low-temperature expression in bacterial hosts: (i) low-temperature adaption of existing mesophilic expression systems, and (ii) development of new psychrophilic hosts. These developments include genetic engineering of the expression cassettes to optimize the promoter/operator systems that regulate heterologous expression. In this addendum we present our efforts in the development of such low-temperature expression systems, and speculate about future advancements in the field and potential applications

Structure/function studies of the NAD⁺-dependent DNA ligase from the poly-extremophile Deinococcus radiodurans reveal importance of the BRCT domain for DNA binding

Bacterial NAD+-dependent DNA ligases (LigAs) are enzymes involved in replication, recombination, and DNA-repair processes by catalyzing the formation of phosphodiester bonds in the backbone of DNA. These multidomain proteins exhibit four modular domains, that are highly conserved across species, with the BRCT (breast cancer type 1 C-terminus) domain on the C-terminus of the enzyme. In this study, we expressed and purified both recombinant full-length and a C-terminally truncated LigA from Deinococcus radiodurans (DrLigA and DrLigA∆BRCT) and characterized them using biochemical and X-ray crystallography techniques. Using seeds of DrLigA spherulites, we obtained ≤ 100 µm plate crystals of DrLigA∆BRCT. The crystal structure of the truncated protein was obtained at 3.4 Å resolution, revealing DrLigA∆BRCT in a non-adenylated state. Using molecular beacon-based activity assays, we demonstrated that DNA ligation via nick sealing remains unaffected in the truncated DrLigA∆BRCT. However, DNA-binding assays revealed a reduction in the affinity of DrLigA∆BRCT for dsDNA. Thus, we conclude that the flexible BRCT domain, while not critical for DNA nick-joining, plays a role in the DNA binding process, which may be a conserved function of the BRCT domain in LigA-type DNA ligases