10 research outputs found
Data from: Types, levels, and patterns of low-copy DNA sequence divergence, and phylogenetic implications, for Gossypium genome types
To explore types, levels, and patterns of genetic divergence among diploid Gossypium (cotton) genomes, 780 cDNA, genomic DNA, and SSR loci were re-sequenced in Gossypium herbaceum (A1 genome), G. arboreum (A2), G. raimondii (D5), G. trilobum (D8), G. sturtianum (C1) and an outgroup, Gossypioides kirkii. Divergence among these genomes ranged from 7.32 polymorphic base pairs per 100 between G. kirkii and G. herbaceum (A1) to only 1.44 between G. herbaceum (A1) and G. arboreum (A2). SSR loci are least conserved with 12.71 polymorphic base pairs and 3.77 polymorphic sites per 100 base pairs, while ESTs are most conserved with 3.96 polymorphic base pairs and 2.06 sites. SSR loci also exhibit the highest percentage of 'extended polymorphisms' (spanning multiple consecutive nucleotides). The A genome lineage was particularly rapidly evolving, with the D genome also showing accelerated evolution relative to the C genome. Unexpected asymmetry in mutation rates was found, with much more transition than transversion mutation in the D genome after its divergence from a common ancestor shared with the A genome. This large quantity of orthologous DNA sequence strongly supports a phylogeny in which A-C divergence is more recent than A-D divergence, a subject that is of much importance in view of A-D polyploid formation being key to the evolution of the most productive and finest-quality cottons. Loci that are monomorphic within A or D genome types, but polymorphic between genome types, may be of practical importance for identifying locus-specific DNA markers in tetraploid cottons including leading cultivars
Supplemental Tables
This file includes four supplemental tables which are relevant to the paper. The supplemental table 1 lists the locus name, GenBank accession number, primer sequence and annealing temperature for all loci studied in this research. Supplemental table 2 is about the diversities revealed in the pair wise comparison including extended and single deletion, insertion, SNP, polymorphic sites, and polymorphic base pairs. Supplemental table 3 is about SNPs, polymorphic sites, and polymorphic base pairs between A and D ancestral genomes. Supplemental table 4 is about diversities revealed in the three way comparison including extended and single deletion, insertion, SNP, polymorphic sites, and polymorphic base pairs
Supplemental Figures
This file includes three supplemental figures which are related to the paper. The figure legends are given below each figure