The relationship between sport-specific training and a perceptuo-motor skills assessment as part of talent identification in young table tennis players (8-10 years)

A perceptuo-motor skills assessment was developed to better estimate the potential of youth table tennis players (8-10 years). Sport-specific training experience might influence the outcomes of this assessment and hinder a fair interpretation. Consequently, the aim of this study was to evaluate the relationship between the training experience (i.e. quantity and quality) and the perceptuo-motor skills assessment outcomes in youth table tennis players. For this purpose, data were collected during the national association’s talent days in the Netherlands (2010-2015). In total, 372 young table tennis players (8-10 years) were assessed by a perceptuo-motor skills questionnaire covering current training (hours/week), total training volume (hours) and quality of the trainer (high versus low). Non-parametric partial correlation analyses showed that training quantity outcomes (i.e. current training and training volume) are significantly associated with the test items of speed while dribbling, aiming at target and eye-hand coordination in both sexes with small to medium effect sizes. The multivariate GLM analyses revealed no significant differences between players receiving high versus low quality training regarding the perceptuo-motor skills assessment. The results indicated only a small transfer of skill and a substantial amount of task specificity; as such it seems legitimate to use the perceptuo-motor skills assessment as part of a talent identification programme. However, it seems sensible to take the training volume into account for a fair interpretation of the estimation of potential, especially when large differences exist between players regarding the training experience. Future studies using a longitudinal design could reveal more about the effect of training

Chromosomal targeting of replicating plasmids in the yeast Hansenula polymorpha

Using an optimized transformation protocol we have studied the possible interactions between transforming plasmid DNA and the Hansenula polymorpha genome. Plasmids consisting only of a pBR322 replicon, an antibiotic resistance marker for Escherichia coli and the Saccharomyces cerevisiae LEU2 gene were shown to replicate autonomously in the yeast at an approximate copy number of 6 (copies per genome equivalent). This autonomous behaviour is probably due to an H. polymorpha replicon-like sequence present on the S. cerevisiae LEU2 gene fragment. Plasmids replicated as multimers consisting of monomers connected in a head-to-tail configuration. Two out of nine transformants analysed appeared to contain plasmid multimers in which one of the monomers contained a deletion. Plasmids containing internal or flanking regions of the genomic alcohol oxidase gene were shown to integrate by homologous single or double cross-over recombination. Both single- and multi-copy (two or three) tandem integrations were observed. Targeted integration occurred in 1-22 % of the cases and was only observed with plasmids linearized within the genomic sequences, indicating that homologous linear ends are recombinogenic in H. polymorpha. In the cases in which no targeted integration occurred, double-strand breaks were efficiently repaired in a homology-independent way. Repair of double-strand breaks was precise in 50-68% of the cases. Linearization within homologous as well as nonhomologous plasmid regions stimulated transformation frequencies up to 15-fold

Similarities and differences between sports subserving systematic talent transfer and development : the case of paddle sports

Objectives: The aim of this study was to investigate similarities and differences for 18 sports toward canoe/kayak in order to identify donorsport and/or multisports, based upon a systematic analysis of the task constraints per sport that are assumed to be either crucial or less important from the coaches' viewpoint. Design: Descriptive survey analysis. Methods: 891 certified coaches from 19 sports valued (0-10; not important at all-very important) 15 characteristics by a questionnaire (Flemish Sports Compass) within their sport. Unique sport-profiles (discriminant analysis - DA) were constructed for 19 sports based on these characteristics. Similarities and differences between canoe/kayak and the other 18 sports were analyzed by means of MANOVAs on anthropometric, physical and motor coordination characteristics. Results: Cross validated DA (rcan = 0.660, Wilks' Lambda = 0.564, p < 0.001) showed that 72.1% of the canoe/kayak coaches were correctly assigned to their sport. For canoe/kayak seven characteristics were valued crucial; dynamic balance (8.51 1.69), core stability (8.45 2.27), pulling power (8.12 +/- 1.68), speed (7.54 +/- 2.07), endurance (7.27 +/- 2.03), stature (6.43 +/- 1.41) and rhythm (6.01 +/- 3.01). Least important characteristics were: flexibility (6.16 +/- 1.75), agility (4.27 +/- 3.10), catching (3.90 +/- 3.22), climbing (2.45 +/- 3.05), jumping (1.81 +/- 2.11), throwing (1.60 +/- 2.24), hitting (.94 +/- 1.77) and kicking (.61 +/- 1.04). Conclusions: This novel approach to determine important characteristics per sport makes identifying similarities and differences between sports possible. Similarities might enlarge talent-pools for possible talent transfers. Differences can help identify sports based on complementary characteristics for the construction of broad motor development programs. From this viewpoint gymnastics can serve as potential donorsport (similarities) for canoe/kayak, while handball and tennis can subserve broad development for young canoe/kayak athletes. (C) 2020 Sports Medicine Australia. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license

Safe and Successful Treatment of Acute Cellular Rejection of an Intestine and Abdominal Wall Transplant With Vedolizumab

Background. Graft survival rates after intestinal transplantation (ITx) are still the lowest in comparison to other solid organ transplants. One of the main reasons is the frequent occurrence of acute cellular rejection (ACR). Vedolizumab is an antibody against α4β7+ integrin involved in gut-homing of T cells which has been approved for inflammatory bowel diseases (IBD). We report its off-label use to treat ACR after ITx. Methods. Following abdominal wall transplantation (AWTx) and ITx, clinical course was followed biochemically. Sequential small intestinal biopsies were taken preceding, during, and after ACR treatment with vedolizumab, following the standard therapy regime for IBD. Rejection was diagnosed histologically, and proinflammatory (α4β7+, interleukin-17+) and regulatory (FoxP3+) T cells were analyzed by immunohistochemistry. Results. ACR in both the ITx and AWTx resolved upon vedolizumab treatment, which was safe, evidenced by clearing an astrovirus and primary cytomegalovirus infection. Only a slight reduction of α4β7+ cells in the mucosa was observed, and α4β7+ and regulatory T cells could still move into the lamina propria upon infection. Conclusions. Vedolizumab is a safe treatment option for ACR after ITx but its mechanism is probably not only based on inhibition of gut-selective T-cell homing

Luminal Preservation Protects the Small Intestine in a Brain-dead Rat Model

Background. Intestinal transplantation depends on donation after brain death (DBD). Luminal preservation (LP) has been beneficial against preservation injury in previous studies in animal models, but none include DBD. This study aims to investigate whether these benefits occur also with DBD. Methods. Wistar rats (male, N = 9) underwent brain death for 2 h. Thereafter, vascular perfusion was done with University of Wisconsin solution (UW). The small intestine was then explanted and randomized into 3 groups: control (empty segment), LP+PEG (with polyethylene glycol 3350 solution), or LP+UW (with UW), treated and tied shut. Ice-cold UW was used for cold storage. Samples were taken at procurement and after 4 (t = 4) and 8 h (t = 8) of preservation. Histopathological scorings were performed for intestinal preservation injury, subepithelial space, absence of epithelial lining, and hemeoxygenase-1 expression. Results. There was low-level mucosal injury (median intestinal preservation injury score 2) at procurement. At t = 4, bowels treated without LP had more damage than LP-treated samples (control score 4, LP+PEG 2 and LP+UW 2, P < 0.001 control versus LP+UW). At t = 8, no benefit of LP was observed (control 2, LP+PEG 3, LP+UW 2). Subepithelial space increased with time and the presence of LP; epithelial lining was better conserved in LP-treated samples. Hemeoxygenase-1 staining showed increased intensity with increased damage, irrespective of treatment. Conclusions. Luminal perfusion of the small intestine with UW or PEG protects the mucosa in brain-dead rats for up to 4 h. Fewer benefits of LP were found than previously described in non-DBD models. To mimic the clinical situation, DBD should be included in future animal studies on intestinal preservation

Ocular and extra-ocular features of patients with Leber congenital amaurosis and mutations in CEP290

Contains fulltext : 108895.pdf (publisher's version ) (Open Access)PURPOSE: This study investigated the centrosomal protein, 290-KD (CEP290) associated genotype and ocular and extra-ocular phenotype in 18 patients with Leber congenital amaurosis (LCA). METHODS: Eighteen patients with LCA from 14 families with mutations in the CEP290 gene were identified with sequencing or with heteroduplex analysis. Ophthalmic examinations were performed on all patients. Scans of the central nervous system were reassessed in three patients and obtained in two. Renal function was evaluated in all patients. Ultrasonography of the kidneys was performed in six patients. RESULTS: Eight patients (from five families) carried the c.2991+1655A>G mutation homozygously. Nine solitary patients carried this variant combined with a nonsense, frameshift, or splice site mutation on the second allele. One new nonsense mutation was identified: c.1078C>T. Fourteen patients (from 12 families) had been completely blind from birth or had light perception. The best-recorded visual acuity was 20/200. Peripheral fundus changes appeared to be progressive with a relatively preserved posterior pole. Novel ophthalmic features for the CEP290 phenotype were Coats-like exudative vasculopathy in two patients, a small chorioretinal coloboma in one patient, and well defined, small, atrophic spots at the level of the retinal pigment epithelium causing a dot-like appearance in five patients. Some CEP290 patients exhibited systemic abnormalities. We found abnormal proprioception in two patients and mild mental retardation in one. One patient was infertile due to immobile spermatozoa. No renal abnormalities were detected. CONCLUSIONS: CEP290-associated LCA has a severe, progressive, and clinically identifiable phenotype. Distinct extra-ocular findings were noted, which may be attributed to ciliary dysfunction

Nutrient content of eight African leafy vegetables and their potential contribution to dietary reference intakes

Nutrient content and potential contribution of one average portion towards nutritional requirements (recommended dietary allowance; RDA) of eight African leafy vegetables (ALVs) was determined. Compared to dark-green leafy vegetables (DGLVs) as sub-group, calcium and magnesium content were similar or considerably higher, vitamin C content was considerably lower, while pigweed had higher potassium content and spider flower similar folate content. All ALVs, except Chinese cabbage, had higher iron content. Black nightshade, pigweed, cowpea and spider flower leaves had higher b-carotene content than DGLVs. For children, pigweed and cowpea leaves emerged as good sources of vitamin A (>75% RDA), followed by spider flower, black nightshade, tsamma melon, Jew’s mallow and pumpkin leaves (50–75% RDA). For iron, pumpkin leaves provided 50–75% RDA. Black nightshade, tsamma melon, pigweed and cowpea leaves contributed 25–50% RDA, with Jew’s mallow, spider flower and Chinese cabbage providing <25% RDA. The ALVs were not a good source of zinc. Most ALVs were nutritionally similar to DGLVs. For most nutrients Chinese cabbage had considerably lower values than the other ALVs. Most of the ALVs can considerably contribute to requirements of vitamin A and, to a lesser extent, iron, both critical nutrients for developing countries.http://www.elsevier.com/locate/jfcahb201

Vibration threshold in non-diabetic subjects

Measuring vibration perception threshold (VPT) accurately classifies and quantifies the severity of loss of vibration perception. A biothesiometer (Bio-thesiometer®; Bio Medical Instrument Co, Ohio, USA) appears to be the most suitable tool to determine VPT due to its low inter-rater variability and low occurence of adaption to the sensation. Different VPT values for a biothesiometer have been described, however, specification on age, height and different measurement locations is currently lacking. The objective of our study was to identify determinants of vibration perception in non-diabetic subjects, in order to provide individualized normal values of VPTs for clinical practice. Measurements of the vibration perception were performed on the big toes, insteps, lateral malleoli, and wrists. A total of 205 healthy subjects were included (108 (52.7%) males) with a median [interquartile range] age of 59 [51;64] (range 21-80) years. Mean height was 174.45 ± 9.20 cm and mean weight was 82.94 ± 14.84 kg, resulting in a mean BMI of 27.19 ± 4.00 kg/m2. In stepwise forward linear regression analyses, age (st. β = 0.51, p < 0.001) and height (st. β = 0.43, p < 0.001) were found to be the independent unmodifiable determinants of the VPT at the big toe. Regression coefficients for quantiles of the determinants age and height were incorporated in the corresponding regression equations. This study provides equations to calculate age- and height-specific normal values for VPT that can be used in clinical practice and in large research studies

Hallux valgus angle as main predictor for correction of hallux valgus

Contains fulltext : 70112.pdf ( ) (Open Access)BACKGROUND: It is recognized that different types of hallux valgus exist. Classification occurs with radiographic and clinical parameters. Severity of different parameters is used in algorithms to choose between different surgical procedures. Because there is no consensus about each parameter nor their cut-off point we conducted this study to analyze the influence of these variables on the postoperative hallux valgus angle. METHODS: After informed consent 115 patients (136 feet) were included. Bunionectomy, osteotomy, lateralization of the distal fragment, lateral release and medial capsulorrhaphy were performed in all patients. Data were collected on preoperative and postoperative HVA, IMA and DMAA measurements. Forty cases were included since our findings in a previous article 1, therefore, current data concern an expanded study group with longer follow-up and were not published before. At least two-year follow-up data were evaluated with logistic regression and independent t-tests. RESULTS: Preoperative HVA was significant for prediction of postoperative HVA in logistic regression. IMA and DMAA were not significant for prediction of postoperative HVA in logistic regression, although they were significantly increased in larger deformities. In patients with preoperative HVA of 37 degrees or more, satisfactory correction could be obtained in 65 percent. The other nine of these 26 patients developed subluxation. CONCLUSION: The preoperative HVA was the main radiological predictor for correction of hallux valgus, correction rate declined from preoperative HVA of 37. IMA and DMAA did have a minor role in patients with preoperative HVA lower than 37 degrees, however, likely contributed to preoperative HVA of 37 degrees or more

Correspondence between neurophysiological and clinical measurements of chemotherapy-induced peripheral neuropathy: secondary analysis of data from the CI-PeriNoms study

Chemotherapy-induced peripheral neuropathy (CIPN) lacks standardized clinical measurement. The objective of the current secondary analysis was to examine data from the CIPN Outcomes Standardization (CI-PeriNomS) study for associations between clinical examinations and neurophysiological abnormalities. Logistic regression estimated the strength of associations of vibration, pin, and monofilament examinations with lower limb sensory and motor amplitudes. Examinations were classified as normal (0), moderately abnormal (1), or severely abnormal (2). Among 218 participants, those with class 1 upper extremity (UE) and classes 1 or 2 lower extremity (LE) monofilament abnormality were 2.79 (95% confidence interval [CI]: 1.28-6.07), 3.49 (95%CI: 1.61-7.55), and 4.42 (95%CI: 1.35-14.46) times more likely to have abnormal sural nerve amplitudes, respectively, compared to individuals with normal examinations. Likewise, those with class 2 UE and classes 1 or 2 LE vibration abnormality were 8.65 (95%CI: 1.81-41.42), 2.54 (95%CI: 1.19-5.41), and 7.47 (95%CI: 2.49-22.40) times more likely to have abnormal sural nerve amplitudes, respectively, compared to participants with normal examinations. Abnormalities in vibration and monofilament examinations are associated with abnormal sural nerve amplitudes and are useful in identifying CIPN