Decisions on Recycling or Waste : How Packaging Functions Affect the Fate of Used Packaging in Selected Swedish Households

The intention of this paper is to learn more about why consumers choose whether or not to recycle, with special attention given to the functions of the packaging itself, in order to provide suggestions for improvements in packaging design, recycling systems and the environmental assessment of different packaging designs. The study focussed on ten households in Sweden that where motivated to participate in the study in order to gain an understanding of the complex matter of this decision-making process. The intention of implementing an interview-based qualitative study was to gain rich data and to reach beyond the respondents' immediate verbal responses. The respondents were interviewed with open-ended questions, which were supported with pictures of packaging; additionally, their waste bins were examined. This explorative study suggests a set of obstacles that cause consumers to dispose of packaging relating to the functions of packaging. The different obstacles that determine whether or not packaging is recycled were organised according to three different themes: the attitude towards cleanliness, the effort required to clean and sort and uncertainties about the best environmental alternative. The different functions of packaging do in fact influence all of the identified themes and; therefore, influence the decisions consumers make with regards to the recycling of specific packaging. The identified packaging functions were easy toseparate different materials, easy to separate different parts, easy to clean,easy to empty, easy to reseal, easy to compress and communication regarding recycling. Consumer behaviour with regards to specific packaging functions and recycling should be further investigated. It should also be considered for inclusion in design processes, to increase the chance of materials being recycled, and in food-packaging life-cycle assessments, to provide results that align more closely with reality

Mechanistic Rationale to Target PTEN-Deficient Tumor Cells with Inhibitors of the DNA Damage Response Kinase ATM

Abstract Ataxia telangiectasia mutated (ATM) is an important signaling molecule in the DNA damage response (DDR). ATM loss of function can produce a synthetic lethal phenotype in combination with tumor-associated mutations in FA/BRCA pathway components. In this study, we took an siRNA screening strategy to identify other tumor suppressors that, when inhibited, similarly sensitized cells to ATM inhibition. In this manner, we determined that PTEN and ATM were synthetically lethal when jointly inhibited. PTEN-deficient cells exhibited elevated levels of reactive oxygen species, increased endogenous DNA damage, and constitutive ATM activation. ATM inhibition caused catastrophic DNA damage, mitotic cell cycle arrest, and apoptosis specifically in PTEN-deficient cells in comparison with wild-type cells. Antioxidants abrogated the increase in DNA damage and ATM activation in PTEN-deficient cells, suggesting a requirement for oxidative DNA damage in the mechanism of cell death. Lastly, the ATM inhibitor KU-60019 was specifically toxic to PTEN mutant cancer cells in tumor xenografts and reversible by reintroduction of wild-type PTEN. Together, our results offer a mechanistic rationale for clinical evaluation of ATM inhibitors in PTEN-deficient tumors. Cancer Res; 75(11); 2159–65. ©2015 AACR.</jats:p

Interaction of angio-associated migratory cell protein with the TPα and TPβ isoforms of the human thromboxane A2 receptor

In humans, thromboxane (TX) A2 signals through the TPα and TPβ isoforms of its G-protein coupled TXA2 receptor (TP) to mediate a host of (patho)physiologic responses. Herein, angio-associated migratory cell protein (AAMP) was identified as a novel interacting partner of both TPα and TPβ through an interaction dependent on common (residues 312-328) and unique (residues 366-392 of TPβ) sequences within their carboxyl-terminal (C)-tail domains. While the interaction was constitutive in mammalian cells, agonist-stimulation of TPα/TPβ led to a transient dissociation of AAMP from immune complexes which coincided with a transient redistribution of AAMP from its localization in an intracellular fibrous network. Although the GTPase RhoA is a downstream effector of both AAMP and the TPs, AAMP did not influence TP-mediated RhoA or vice versa. Small interfering RNA (siRNA)-mediated disruption of AAMP expression decreased migration of primary human coronary artery smooth muscle cells (1° hCoASMCs). Moreover, siRNA-disruption of AAMP significantly impaired 1° hCoASMC migration in the presence of the TXA2 mimetic U46619 but did not affect VEGF-mediated cell migration. Given their roles within the vasculature, the identification of a specific interaction between TPα/TPβ and AAMP is likely to have substantial functional implications for vascular pathologies in which they are both implicated.Science Foundation IrelandHealth Research Boardke, -SB01/09/201