PENGARUH INTELLECTUAL CAPITAL DAN PENGUNGKAPAN CORPORATE SOCIAL RESPONSIBILITY TERHADAP KINERJA PERUSAHAAN

Penelitian ini bertujuan untuk menguji pengaruh intellectual capital dan pengungkapan corporate social responsibility terhadap kinerja perusahaan. Intellectual capital diukur dengan Value Added Intellectual Capital (VAIC), pengungkapan corporate social responsibility diukur dengan Corporate Social Responsibility Index (CSRI), dan kinerja perusahaan diukur dengan menggunakan Return On Equity (ROE). Populasi dalam penelitian ini adalah perusahaan manufaktur yang terdaftar di Bursa Efek Indonesia (BEI) selama periode 2013-2016 data dipilih berdasarkan metode purposive sampling, sehingga diperoleh sampel sebanyak 50 perusahaan manufaktur. Metode analisis yang digunakan adalah analisis regresi linear berganda dengan aplikasi SPSS (Statistical Product and Service Solution). Hasil penelitian menunjukkan bahwa (1) intellectual capital berpengaruh positif terhadap kinerja perusahaan. (2) pengungkapan corporate social responsibility tidak berpengaruh terhadap kinerja perusahaan. Kata kunci: intellectual capital, pengungkapan corporate social responsibility, kinerja perusahaa

Membangun Kepemimpinan Kuat Dan Tangguh Pada Pengembangan Sumber Daya Aparatur Pemerintah Daerah Melalui Pendidikan Dan Pelatihan

Strong and resilient leadership in the dynamics of advances in Science Technology and Science (IPTEKS) and technology is always expected by the community, especially to support the goal of increasing the quality of national development in the area of Information and Communication (ICT) today. Leadership Resources of local government apparatus in Indonesia need to be developed using education and training methods. This research uses descriptive analytic method and implemented literature study. The implementation of leadership tasks in the regions in the future is faced with several main issues that influence each other, namely science and technology as a concept of developing development forces faced with the dynamics of national, regional, and global security. Theories that are considered relevant to be applied in the development of Regional Leaders Others are Behavioral Theory and Participative Theory. The research results discuss the Regional Heads and the Competencies of Leadership and Educational Personnel of the Institute for Regional Leadership Development. The Ki Hadjar Dewantara Leadership Trilogy, which has been implemented by the TNI until now, can be used as a permanent leadership development method in the regions. This research recommends a leadership development approach through three aspects, namely, the Aspects of Regional Leadership Development, Aspects of Increasing the Competence of Educational Personnel of the Leadership Training Institute, and the Aspects of Facilities. and Infrastructure

MEMBANGUN KEPEMIMPINAN KUAT DAN TANGGUH PADA PENGEMBANGAN SUMBER DAYA APARATUR PEMERINTAH DAERAH MELALUI PENDIDIKAN DAN PELATIHAN

In the case of the economic recession of Covid-19 Pandemic nowadays, strong and resilient leadership in the dynamics of advances in Science Technology and Science (IPTEKS) and technology is always expected by the community, especially to support the goal of increasing the quality of national development in the area of Information and Communication (ICT) today. Leadership Resources of local government apparatus in Indonesia need to be developed using education and training methods. This research uses the descriptive-analytic method and implemented a literature study. The implementation of leadership tasks in the regions in the future is faced with several main issues that influence each other, namely science and technology as a concept of developing development forces faced with the dynamics of national, regional, and global. Theories that are considered relevant to be applied in the development of Regional Leaders Others are Behavioral Theory and Participative Theory. The research results discuss the Regional Heads and the Competencies of Leadership and Educational Personnel of the Institute for Regional Leadership Development. The Ki Hadjar Dewantara Leadership Trilogy, which has been implemented nationally until now, can be used as a permanent leadership development method in the regions. This research recommends a leadership development approach through three aspects, namely, the Aspects of Regional Leadership Development, Aspects of Increasing the Competence of Educational Personnel of the Leadership Training Institute, and the Aspects of Facilities. and Infrastructure

SPECIFICITY OF ANTIBODY BOVINE ZONNA PELLUCIDAE 3 (ANTI-bZP3) TO RABBIT ZP3 BASED ON bZP3 AS CONTRACEPTIVE ANTIGENS

Zonna pellucidae can be develop as antigen potential candidates based on reversible immunocontraceptive vaccines. Immunogenic sites of bovine zonna pellucidae 3 (bZP3) could stimulated the presence of anti-bZP3 which be located on rabbit ZP and inhibit sperm-egg interaction on fertilization process. Purpose of this research is to detect spesific binding anti-bZP3 to rabbit oocytes using dot blotting and ELISA method. Sub cutan induction of bZP3 with Freund's adjuvant, CFA (Complete Freund's Adjuvant) for initial immunization and following by IFA (Incomplete Freund's Adjuvant) at the 14th day and 39th day. Control female rabbit injected by Tris-Cl buffer diluted in Freund's adjuvant without bZP3 antigen. Rabbit serum injected to rat for producing Rat Anti Rabbit Anti-bZP3. This research concludes spesific binding of anti-bZP3 with increasing purple colour on dot blotting methods. Anti-bZP3 increasing on 24th day and 31th day and still until 48th day. Measurement with ELISA methods showed increased titer on OD405. Highest titer showed on 31th day post immunization. Anti-bZP3 synthetized by bZP3 induced on rabbit detectable by immunohistochemistry methods on late primary oocytes, early secondary oocytes, growing secondary oocytes, and oocytes on de Graaf folicular phase. Keywords: Dot blotting, ELISA, bZP3, anti-bZP

Optimizing Collagenase, Fetal Bovine Serum, and Insulin to Isolate, Proliferate, and Differentiate Rat Preadipocyte in vitro

BackgroundThe aim of this study is to develop a method for optimal in vitro proliferation and differentiation of rat adipocyte. MethodsPreadipocyte were isolated from omentum of Rattus norvegicus Wistar using Collagenase type I dan II (Sigma). Cells were cultured in M199 culture containing either 0%, 8% or 10% Fetal Bovine Serum (FBS). Insulin were added into the media once the cells attached to the culture plate Proliferating and differentiated cells were counted and analysed by using Oil Red O dan Hematoxylen (HE) staining. Results This study demonstrated that adipocyte could be isolated using Collagenase type I but not type II. The addition of 10% FBSsignificantly increased the number of preadipocyte and differentiation of adipocyte more than those of 8% FBS and without FBS. The timing of FBS addition was best performed on day 8 using 10% FBS. Specific adipocyte staining using Oil Red O revealed thatthere were core lipids in mature adipocyte. ConclusionsCollagenase tipe I could be used to isolate preadipocyte cells. Supplementation of culture media with 8-10% FBS could enhance the in vitro proliferation of preadipocyte. Standard media M199 containing 10% FBS and insulin may provide an environment to differentiate preadipocyte specifically into adipocyte in vitro

Study of some biochemical parameters in young men as effected by Ramadan Fasting

The effect of Ramadan fasting on some blood parameters i.e. serum glucose, urea, uric acid, lipids and proteins, were investigated on young men in Ar Rohmah Islamic dormitory. Nineteen normal and healthy students aging between 12-25 years, residing in the Islamic dormitory, voluntarily to participated in the study. Blood samples were obtained from the volunteers on the 1st and 26th day of Ramadan and analyzed for the aforementioned biochemical parameters. A non-significant effect of Ramadan fasting was observed on most of the parameters studied. However, serum urea, triglycerides, total cholesterol and LDL-cholesterol were reduced significantly (p0.05) but remained within the physiological limits. Decrease in blood urea has been attributed to the effect of at least protein and triglycerides intake to increase lipolytic effect. The reduction in serum cholesterol and LDL is a beneficial effect of Ramadan fasting. The results of the study indicated thatRamadan fasting is quite safe for normal healthy adults

Modifying Effect of Anthocyanin from Purple Sweet Potatoes on Visceral Fat Tissue Inflammation and Liver Oxidative Stress in Psychological Stress-Induced Mice

Psychological stress generates inflammation and oxidative stress in various tissues. Visceral fat as the target site of inflammation is then correlated with stress-induced obesity. Redox imbalance following inflammation also has prominent impacts on hepatic tissue. Therefore, the development of anti-inflammatory and antioxidant properties from plant bioactive compounds is necessary to be investigated. Anthocyanin (ANC) from various plants is previously described as a powerful anti-inflammatory and antioxidant even though its effect in psychological stress remains underexplored. Purple sweet potatoes (PSP) has ANC as its natural pigment. Previous studies revealed the potential effect of ANC from PSP on behavior and an antidepressant candidate. Thus, this study was purposed to determine the effect of ANC from PSP on inflammation and oxidative stress in visceral fat and hepatic tissue respectively. A total of 25 adult male BALB/c mice were assigned into groups of control, stress, stress+ANC 10 mg/kgBW, stress+ANC 20 mg/kgBW and stress+ANC 40 mg/kgBW. Restraint stress was applied 2 hours/day for 14 days. Enzyme-linked immunoassay (ELISA) was conducted to measure level of IL-6 and IL-10 in visceral fat as well as SOD and MDA from hepatic tissue. The results demonstrated that the supplementation of ANC reduces the level of IL-6 cytokine (p=0.005), tends to increase IL-10 (p=0.612), reduces hepatic SOD (p=0.03), and does not significantly affect the level of hepatic MDA (p=0.432). Both ratios of IL-6/IL-10 and SOD/MDA were reduced following ANC administration. Total ANC extracts are suggested to have a potential role of resisting inflammation and oxidative stress in the psychologically stressed model. Further studies are necessary to evaluate the benefits of ANC from PSP in other peripheral organs under psychological stress exposure

Enhanced anticancer effect of cetuximab combined with ethanol extract of Volvariella volvacea in colorectal cancer targeted TOP2A and PPARγ

Context: Colorectal cancer (CRC) is a major global health concern as innovative therapeutic strategies to enhance treatment outcomes. This study investigates the therapeutic potential of the ethanol extract derived from Volvariella volvacea in combination with cetuximab for CRC. Aims: To evaluate the inhibition of topoisomerase II alpha (TOP2A) and include the peroxisome proliferator-activated receptor gamma (PPARγ), a key regulator of cell growth and differentiation. Methods: The method used computational modeling and molecular docking to assess the binding affinities and interactions between the bioactive components, cetuximab, TOP2A, and PPARγ. In vitro experiments were conducted using CRC cell lines. Cells were treated with cetuximab alone or in combination with V. volvacea extract. Cell viability and immunofluorescence were conducted to evaluate the effect of combination therapy on TOP2A and PPARγ. Results: The results unveiled compelling binding interactions, suggesting the potential for dual targeting of TOP2A and PPARγ. The results demonstrated a reduction in cell viability, downregulation of TOP2A, and modulation of PPARγ. These findings indicate the potential of V. volvacea extract as an adjuvant therapy to cetuximab in CRC. Targeting both TOP2A and PPARγ enhances the efficacy of CRC treatment. Conclusions: Moreover, it highlights the importance of harnessing natural compounds and molecular insights to develop innovative combinatorial therapies for complex diseases like CRC

Physalis angulata Leaf Ethanol Extract Reduces Oxidative Stress and Improves Endothelial Progenitor Cells in L-NAME-Induced Hypertensive Rats

This study aimed to evaluate the effects of ciplukan (Physalis angulata L.) leaf ethanol extract on L-NG-nitroarginine methyl ester (L-NAME)-induced hypertensive rats. We randomly divided twenty-five Wistar rats into five groups. The sham group was given a PBS injection. The hypertensive group was injected with L-NAME on days 1 to 28. Three groups of hypertensive rats were given the extract on days 4 to 28. Blood pressure was measured using the tail-cuff method on days 0, 4, 10, and 27. The endothelial progenitor cells (EPCs) in the blood were measured by flow cytometry as a percentage of circulating angiogenic cells (CACs, CD34+/CD309+/CD45+) and endothelial colony-forming cells (ECFCs, CD34+/CD309+/CD45-). Serum NO and MDA levels, as well as serum SOD activity, were measured colorimetrically. Serum TNF-α levels were measured by the ELISA method. The ciplukan leaf extract reduced systolic and diastolic blood pressure, reduced the percentage of EPCs in the blood, increased serum NO levels, reduced MDA levels, increased serum SOD activity, and reduced serum TNF-α levels in L-NAME-induced hypertensive rats. It is concluded that ciplukan ethanol leaf extract exerts protective effects on L-NAME-induced hypertensive rats. These study results can strengthen the scientific basis of using ciplukan leaf ethanol extract to treat hypertension