Impoliteness Strategies Used on Online Comments in an Indonesian Football Website

This research investigates linguistic impoliteness used in online football comments through the examination of impoliteness strategies proposed by Jonathan Culpeper. It examines how impoliteness strategies are used on online comments and what strategy mostly used by Indonesian participants in Okezone, an Indonesian football website. The research uses descriptive qualitative method supported by quantitative data. First, the writer found that Indonesian participants mostly used positive impoliteness strategy to express their negative attitude on giving comments. Second, there are four out of five impoliteness strategies used by Indonesian participants. Withhold politeness strategy is excluded

PRODUCTION AND CHARACTERIZATION OF CRUDE INTRACELLULER PHYTASE FROM RECOMBINANT BACTERIA pEAS1AMP

This research was aimed at producing a crude intracellular phytase characterized from recombinant bacteria. The recombinant bacteria (pEAS1AMP) was produced by way of transforming pET-22b(+) +pEAS1 into competent E. coli BL21 and E. coli BL21(DE3) cells. Crude intracellular phytase production was induced using 1,5 mM Isopropyl-β-D-thiogalactopyranosid (IPTG). Recombinant bacteria product and enzyme activity test followed the Sajidan method. E. coli BL21(+)pEAS1 and E. coli BL21 (DE3)(+)pEAS1 recombinant bacteria showed growth after 20 hours and 10 hours of transformation. Phytase activity of E. coli BL21 (DE3)(+)+pEAS1 showed higher than those of E. coli BL21(+)+pEAS1. Crude intracellular phytase of pEAS1AMP recombinant bacteria has an optimum activity at pH 5, 40o C, incubation period of 60 minutes, substrate concentration of 2%, molecular weight (MW) of 47.3 kDa, Km = 15.91 υM and Vm = 2.41 υM/second. Mg2+ acts as a cofactor but Fe3+ (10-4 M) acts as an inhibitor. Keywords: bacteria recombinant pEAS1AMP, competent cells, crude intracellular phytas

In Vitro Stability of Phytase from Recombinant Bacteria E. Coli BL21 (DE3) EAS1-AMP

The objective of the research was to inquire the Km, Vm, activity, intracellular phytase stability exposed to pH variation, temperature variation and protease (pepsin and pancreas) in vitro. The phytase was produced from recombinant bacteria E. coli BL21(DE3) EAS1-AMP using 1.5 mM IPTG as inducer. Intracellular enzyme was extracted via freeze shock and centrifugation. Pure enzyme was acquired through NI-NTA agarose column. The enzyme was then tested for Km, Vm, phytase activity and stability against pH, temperature and protease. Treatment levels for stability against protease were P0: without protease, P1: addition of pepsin, P2: addition of pepsin and pancreas, and the data were statistically analyzed using analysis of variance of one-way Completely Randomized Design. Crude intracellular phytase had Vm 6.39 υM/sec, Km 34.82 υM, and 277 units activity. Intracellular phytas was stable at pH 4–6 and 0–550 C. Protease level influenced the activity of intracellular phytase (P<0.05). Intracellular phytase was stable against pepsin but not pancreas. Keywords: Km, Vm, activity, intracellular phytase, pH, temperature, protease

Penyelesaian Sengketa antara Pengguna Jasa dengan Penyedia Jasa dalam Hal Perbedaan Interpretasi Klausul Kontrak Tentang Perubahan Desain Konstruksi Bangunan (Studi Kontrak antara PT Hutama Karya (Persero) dengan PT Petrokimia Gresik )

Perubahan desain konstruksi bangunan yang diajukan oleh PT Petrokimia Gresikselaku penyedia jasa terhadap PT Hutama Karya (Persero) pada saat pelaksanaankontrak konstruksi proyek Engineering Procurement Construction (EPC)pengembangan pelabuhan. Perubahan desain konstruksi tersebut dilakukan karenadesain yang dituangkan dalam pembangunan secara riil tidak sesuai dengankehendak pihak PT Petrokimia Gresik. Perubahan desain konstruksi bangunantersebut mengakibatkan PT Hutama Karya mengajukan klaim penambahan biayadan waktu kepada PT Petrokimia Gresik. Akan tetapi PT Petrokimia Gresikmenolak klaim tersebut karena Perubahan desain konstruksi bangunan tersebutadalah resiko PT Hutama Karya yang tidak seharusnya mendapatkan tambahanbiaya dan waktu. Penelitian ini adalah penelitian hukum empiris denganpendekatan yuridis sosiologis. Lokasi penelitian yang diakukan dalam penelitianini adalah di PT Hutama Karya (Persero) kantor wilayah VII Kota Surabaya danPT Petrokimia Gresik, serta Jurusan teknik sipil Fakultas Teknik UniversitasBrawijaya Malang, dengan teknik purposive sample yang akan digunakan adalahsalah satu anggota penyelesai sengketa PT Hutama Karya (Persero) dan PTPetrokimia Gresik, serta salah satu dosen teknik sipil Universitas Brawijaya. Dataprimer, sekunder, dan tersier yang diperoleh akan di analisis dengan metodedeskriptif kualitatif. Permasalahan yang diangkat adalah faktor-faktor penghambatpenyelesaian sengketa antara PT Hutama Karya (Persero) dengan PT PetrokimiaGresik dalam perbedaan interpretasi klausul kontrak tentang Perubahan desainkonstruksi bangunan sulitnya menentukan suatu Perubahan desain konstruksiadalah resiko PT Hutama Karya (Persero) atau resiko PT Petrokimia Gresikselaku pengguna jasa. Upaya yang dilakukan oleh PT Hutama Karya (Persero)dengan PT Petrokimia Gresik dalam proses penyelesaian sengketa denganmembentuk dan menggunakan tim peneliti ahli konstruksi bangunan dariakademisi Teknik Sipil Institut Teknologi Bandung dan Institut TeknologiSepuluh Nopember Surabaya yang bersifat independen. Industri jasa konstruksi dan pengguna jasa konstruksi hendaklah dalam melakukan penyusunan kontrakdan kelengkapannya secara lebih cermat, teliti, dan tekun serta menyelesaikansengketa dalam perbedaan interpretasi pada klausul kontrak konstruksi sesuaidengan aturan yang berlaku

Pengaruh Gaya Kepemimpinan Transaksional dan Kompensasi terhadap Komitmen PT Amerta Giri Lestari YOGYAKARTA

This study aims to determine the effect of transactional leadership on commitment to PT Amarta Giri Lestari. To know influence of perception of compensation influence to commitment and effect of transactional leadership and perception of compensation simultaneously affect the commitment. The variables of this research are transactional leadership style, compensation and commitment. The population in this research is employees of PT Amarta Giri Lestari Yogyakarta, amounting to 50 people and also as a sample. Since all of the population were used as samples, this study used the census method. Method of taking data using questioner. The analysis technique used is multiple linear regression with 5% significance level. The result of the research is regression equation Y = 0,413X1 + 0,442X2. Transactional leadership has a positive and significant influence on the commitment of PT Amarta Giri Lestari Yogyakarta, this is indicated by the probability value (0.001) is smaller than the significant level of 0.05. The perception of compensation has a positive and significant impact on the commitment of PT Amarta Giri Lestari Yogyakarta, this is indicated by the probability value (0,000) smaller than the significant level of 0.05. Transactional leadership, and perception of compensation affect simultaneously to the commitment of PT Amarta Giri Lestari Yogyakarta, this is indicated by a significant level of 0.000 &lt;0.05 (p &lt;0.05). Commitment influenced Transactional leadership and perception of compensation by 53.1%. While the rest equal to 46.9% influenced by other factors

Agrowisata Development Strategy In Tourist Village Kembangarum, Donokerto Village, Turi, Sleman District

:Agrowisata in tourist village Kembangarum have different tourism resources, hence the qualitative study aims to (1) know factors affecting development agrowisata in tourist village kembangarum, (2) know the role of each stakeholders, (3) strategy implemented in the development of agrowisata in tourist village Kembangarum, (4) priority strategy applied in the development of agrowisata in tourist village Kembangarum. The methods used is a method of descriptive. The sample collection use purposive sample were the ones who related in development. The result of this research is, factor that influences development agrowisata in tourist village Kembangarum consisting of potential natural resources and fresh typical traditional, potential human resources derived from the aboriginal peoples of Donokerto having productive age, tourism potential offer a choice variety of education tourism, potential geographical at the location strategic, potential demographic this city tinged the dynamics of students and college students who coming from many areas, potential psikografis the number of visitors come of the city, outside of cities and foreign. Agrowisata tourist village Kembangarum managed by manager involving the public role Donokerto, the government and partner. Strategy implemented in the development of agrowisata tourist village Kembangarum based on the analysis of interactive as follows: improve the quality of packaging salak, community empowerment aimed at increasing public welfare, reflect typicality and maintain tradition as well as to create potential new tour

Pelaksanaan Bimbingan dan Konseling di Sekolah Menengah Pertama Luar Biasa Negeri Lampung Timur

Tujuan penelitian ini untuk mengetahui bagaimana perencanaan bimbingan dan konseling di Sekolah Menengah Pertama Luar Biasa,&nbsp; pelaksanaan layanan bimbingan dan konseling di Sekolah Menengah Pertama Luar Biasa, dan evaluasi pelaksanaan layanan bimbingan dan konseling di Sekolah Menengah Pertama Luar Biasa Negeri Lampung Timur Tahun Pelajaran 2019/2020. Jenis penelitian ini adalah deskriptif kualitatif. Subjek data adalah guru bimbingan dan konseling dan guru kelas. Data yang diperoleh peneliti melalui metode wawancara dan observasi. Analisis data menggunakan model miles yaitu pengumpulan data, reduksi data, penyajian data dan menarik kesimpulan. Pengujian keabsahan dapat dilakukan dengan cara triangulasi sumber.Kesimpulan dalam penelitian ini adalah (1) perencanaan guru bimbingan dan konseling melakukan persiapan dan perencanaan seperti melakukan asesment kebutuhan yang dibuat sendiri sesuai dengan aspek-aspek aktivitas kegiatan sehari-hari dan keempat bidang layan terdiri dari pribadi sosial, belajar, karir pada anak berkebutuhan khusus, hasil asesmen dianalisis, penyusunan rencana pelaksanaan layanan, penyusunan program. (2) pelaksanaan layanan guru bimbingan dan konseling melaksanakan layanan informasi secara kelas besar dengan metode ceramah dan layanan konseling individu (3) pelaksanaan evaluasi guru bimbingan dan konseling melakukan pengukuran kinerja dengan menilai dan menindaklanjuti metode dan strategi yang digunakan, penyusunan laporan pelayanan bimbingan dan konseling, dilaporkan kepada kepala sekolah. &nbsp; The purpose of this study was to determine how the planning and the evaluation of the implementation of guidance and counseling services in Special Public Junior High School, East Lampung in Academic Year 2019/2020. The type of this research is descriptive qualitative. The data subjects are guidance and counseling teachers and homeroom teachers. The data obtained by researchers through interviews and observation. The data analysis used the Miles model; collection, data reduction, data presentation, and drawing conclusions. The validity testing was done by triangulating sources. The conclusions in this study are (1) teachers' planning in guidance and counseling and preparation such as conducting self-assessment needs according to aspects of daily activities and the four service areas consisting of social personal, learning, a career in children with special needs, results of the analysis are analyzed, preparation of service implementation plans, preparation of programs. (2) the teachers' implementation of guidance and counseling services such as implementing information services in a large class with lecture methods and individual counseling services (3) the evaluation implementation of guidance and counseling teachers in performance measurements by assessing and following up on the methods and strategies used, preparation of guidance and counseling service reports and reported to the school principal

Penyisihan Logam Pada Lindi Dengan Sistem Sub-surface Constructed Wetland

Salah satu hasil dari proses pengolahan sampah yang dilakukan di TPA adalah air lindi. Sebagian besar sampah yang diolah di TPA Batu Layang berasal dari limbah domestik, walaupun demikian, di dalam air lindi tersebut juga memiliki kandungan logam. Keberadaannya di lingkungan harus dihindari karena apabila logam berat masuk ke dalam rantai makanan dalam kadar tertentu dapat mengganggu kesehatan. Tujuan dari penelitian ini adalah mengetahui seberapa besar efisiensi penyisihan logam Cu, Zn, dan Fe pada lindi dengan menggunakan sistem Sub-surface Constructed Wetland menggunakan tanaman Cyperus papyrus dan Echinodorus palaefolius, membandingkan besar efisiensi antara tanaman Cyperus papyrus dan Echinodorus palaefolius dalam penyisihan logam pada lindi dan menentukan waktu detensi yang efektif untuk menurunkan kadar logam pada lindi. Setelah dilakukan penelitian, efisiensi penyisihan logam Cu, Fe dan Zn pada lindi dalam bak I dengan tanaman echinodorus paleofolius berdasarkan hasil analisis sampel awal lindi masing-masing sebesar 34.5%, 85.5% dan 94.2% dan pada bak II dengan tanaman cyperus papyrus masing-masing sebesar -3.4%, 76.4% dan 91.4%. Apabila berdasarkan hasil analisis effluent hari ke-0, maka efisiensi penyisihan logam pada bak I masing-masing sebesar 87.7%, 99.3% dan 95.5% sedangkan pada bak II masing-masing sebesar 75.9%, 98.8% dan 93.4%. Tanaman echinodorus paleofolius mampu menyerap logam Cu, Fe dan Zn dengan efisiensi penyerapan masing-masing sebesar 82.9%, 92.3% dan 90.5%, lebih banyak dibandingkan dengan tanaman cyperus papyrus yang memiliki efisiensi penyerapan masing-masing 71.5%, 90.8% dan 87.6%. Waktu detensi yang efektif dalam penurunan logam Cu, Fe dan Zn pada bak I dengan tanaman echinodorus paleofolius adalah 6 hari, sedangkan pada bak II dengan tanaman cyperus papyrus, waktu detensi untuk ketiga logam adalah 9 hari